Abstract:
:The African swine fever virus protein A238L inhibits activation of NFAT transcription factor by binding calcineurin and inhibiting its phosphatase activity. NFAT controls the expression of many immunomodulatory proteins. Here we describe a 14-amino-acid region of A238L that is needed and sufficient for binding to calcineurin. By introducing mutations within this region, we have identified a motif (PxIxITxC/S) required for A238L binding to calcineurin; a similar motif is found in NFAT proteins. Peptides corresponding to this domain of A238L bind calcineurin but do not inhibit its phosphatase activity. Binding of A238L to calcineurin stabilizes the A238L protein in cells. Although A238L-mediated suppression of NF-kappaB-dependent gene expression occurs by a different mechanism, the A238L-calcineurin interaction may be required to stabilize A238L.
journal_name
J Viroljournal_title
Journal of virologyauthors
Miskin JE,Abrams CC,Dixon LKdoi
10.1128/jvi.74.20.9412-9420.2000subject
Has Abstractpub_date
2000-10-01 00:00:00pages
9412-20issue
20eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
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