Abstract:
:The ascomycete Botrytis porri causes clove rot and leaf blight of garlic worldwide. We report here the biological and molecular features of a novel bipartite double-stranded RNA (dsRNA) mycovirus named Botrytis porri RNA virus 1 (BpRV1) from the hypovirulent strain GarlicBc-72 of B. porri. The BpRV1 genome comprises two dsRNAs, dsRNA-1 (6,215 bp) and dsRNA-2 (5,879 bp), which share sequence identities of 62 and 95% at the 3'- and 5'-terminal regions, respectively. Two open reading frames (ORFs), ORF I (dsRNA-1) and ORF II (dsRNA-2), were detected. The protein encoded by the 3'-proximal coding region of ORF I shows sequence identities of 19 to 23% with RNA-dependent RNA polymerases encoded by viruses in the families Totiviridae, Chrysoviridae, and Megabirnaviridae. However, the proteins encoded by the 5'-proximal coding region of ORF I and by the entire ORF II lack sequence similarities to any reported virus proteins. Phylogenetic analysis showed that BpRV1 belongs to a separate clade distinct from those of other known RNA mycoviruses. Purified virions of ~35 nm in diameter encompass dsRNA-1 and dsRNA-2, and three structural proteins (SPs) of 70, 80, and 85 kDa, respectively. Peptide mass fingerprinting analysis revealed that the 80- and 85-kDa SPs are encoded by ORF I, while the 70-kDa SP is encoded by ORF II. Introducing BpRV1 purified virions into the virulent strain GarlicBc-38 of B. porri caused derivative 38T reduced mycelial growth and hypovirulence. These combined results suggest that BpRV1 is a novel bipartite dsRNA virus that possibly belongs to a new virus family.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wu M,Jin F,Zhang J,Yang L,Jiang D,Li Gdoi
10.1128/JVI.00292-12subject
Has Abstractpub_date
2012-06-01 00:00:00pages
6605-19issue
12eissn
0022-538Xissn
1098-5514pii
JVI.00292-12journal_volume
86pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1978-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.21.1.358-365.1977
更新日期:1977-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02339-07
更新日期:2008-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.686-690.1990
更新日期:1990-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.3.936-941.1979
更新日期:1979-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2508-2514.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.472-485.2005
更新日期:2005-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.5157-5160.1992
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.6.1208-1219.1972
更新日期:1972-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3332-3340.2006
更新日期:2006-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.677-687.1978
更新日期:1978-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2825-2831.1999
更新日期:1999-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1991-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01772-15
更新日期:2015-11-11 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9352-9365.2004
更新日期:2004-09-01 00:00:00
abstract::Ebola virus (EBOV) causes severe hemorrhagic fever, for which therapeutic options are not available. Preventing the entry of EBOV into host cells is an attractive antiviral strategy, which has been validated for HIV by the FDA approval of the anti-HIV drug enfuvirtide. To identify inhibitors of EBOV entry, the EBOV en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01456-10
更新日期:2011-04-01 00:00:00