Abstract:
:Escherichia coli RNA polymerase has been shown to bind to a limited number of Hind and Hae III restriction enzyme fragments. On S13 replicative form DNA there are three major binding sites, and the locations correlate with promoter sites at the beginning of genes a and B and a site overlapping gene D and the beginning of gene E. Two less definite binding sites have been localized, one in gene F and one at the gene G-H junction. In phiX174 replicative form DNA, five sites, each with apparently similar binding properties, have been located, four of which correspond exactly to binding sites in S13. One site, at the beginning of the B gene, could not be assigned to exactly the same location found in S13. This was due in part to differences in the restriction cleavage maps in the area of the DNA and possibly to the higher background of nonspecific binding in the phiX174 experiments. The location of two of the phiX174 sites at the beginning of genes A and D-E corresponds very well with transcription data, but the site at the start of the B gene indicates the promoter site is closer to the initiation sequence of the B protein than was previously suggested on the basis of transcription data.
journal_name
J Viroljournal_title
Journal of virologyauthors
Rassart E,Spencer JHdoi
10.1128/JVI.27.3.677-687.1978subject
Has Abstractpub_date
1978-09-01 00:00:00pages
677-87issue
3eissn
0022-538Xissn
1098-5514journal_volume
27pub_type
杂志文章abstract::Epstein-Barr virus (EBV), a member of human gammaherpesvirus, infects mainly B cells. EBV has two alternative life cycles, latent and lytic, and is reactivated occasionally from the latent stage to the lytic cycle. To combat EBV-associated disorders, understanding the molecular mechanisms of the EBV lytic replication ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00975-17
更新日期:2017-11-14 00:00:00
abstract::Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus related to rhabdoviruses and paramyxoviruses. Unlike animal viruses of these two families, BDV transcribes RNAs in the nuclei of infected cells and produces high levels of transcripts containing multiple open reading frames. Previous Northern blot ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5007-5012.1994
更新日期:1994-08-01 00:00:00
abstract::MicroRNAs (miRNAs) are key regulators of gene expression in higher eukaryotes. Recently, miRNAs have been identified from viruses with double-stranded DNA genomes. To attempt to identify miRNAs encoded by herpes simplex virus 1 (HSV-1), we applied a computational method to screen the complete genome of HSV-1 for seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00200-06
更新日期:2006-06-01 00:00:00
abstract::Historically, the adenoviral E3 region was found to be nonessential for viral replication in vitro. In addition, adenoviruses whose genome was more than approximately 105% the size of the native genome were inefficiently packaged. These profound observations were used experimentally to insert transgenes into the adeno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5455-5465.2005
更新日期:2005-05-01 00:00:00
abstract::A soluble polymerase-template complex prepared from poliovirus-infected cells was found to incorporate radioactive UTP into trichloroacetic acid-insoluble RNA linearly for 8 h in the presence of ATP and Mg2+. Radioactive CTP or GTP was not incorporated under identical conditions. Nearest-neighbor analysis of the in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.61-68.1977
更新日期:1977-01-01 00:00:00
abstract::The regulatory proteins Nef, Rev, and Tat of human immunodeficiency virus type 1 (HIV-1) are attractive targets for vaccine development, since induction of effective immune responses targeting these early proteins may best control virus replication. Here we investigated whether vaccination with biologically active Tat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3800-3809.2002
更新日期:2002-04-01 00:00:00
abstract:UNLABELLED:Enteroviruses (EVs) are a genetically and antigenically diverse group of viruses infecting humans. A mostly distinct set of EV variants have additionally been documented to infect wild apes and several, primarily captive, Old World monkey (OWM) species. To investigate the prevalence and genetic characteristi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00088-14
更新日期:2014-06-01 00:00:00
abstract::A polymorphism in the gene encoding CCR2 is associated with a delay in progression to AIDS in human immunodeficiency virus (HIV)-infected individuals. The polymorphism, CCR2-64I, changes valine 64 of CCR2 to isoleucine. However, it is not clear whether the effect on AIDS progression results from the amino acid change ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2450-2459.1999
更新日期:1999-03-01 00:00:00
abstract::Kilham rat virus (KRV) contains linear, single-stranded DNA in the virion. The fate of radioactive viral DNA was followed after infection of monolayer cells. Within 60 min after infection of cells, 28 to 42% of the parental viral DNA is converted to a new form. This new DNA form is believed to be double stranded and l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.2.299-305.1973
更新日期:1973-02-01 00:00:00
abstract::Pigs are permissive to both human and avian influenza viruses and have been proposed to be an intermediate host for the genesis of pandemic influenza viruses through reassortment or adaptation of avian viruses. Prospective virological surveillance carried out between March 1998 and June 2000 in Hong Kong, Special Admi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9679-9686.2001
更新日期:2001-10-01 00:00:00
abstract::The structure of a complex between human rhinovirus 2 (HRV2) and the Fab fragment of neutralizing monoclonal antibody (MAb) 3B10 has been determined to 25-A resolution by cryoelectron microscopy and three-dimensional reconstruction techniques. The footprint of 3B10 on HRV2 is very similar to that of neutralizing MAb 8...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4396-4402.1998
更新日期:1998-05-01 00:00:00
abstract::The chimpanzee monoclonal antibody (MAb) 5H2 is specific for dengue virus type 4 (DENV-4) and neutralizes the virus at a high titer in vitro. The epitope detected by the antibody was mapped by sequencing neutralization escape variants of the virus. One variant contained a Lys174-Glu substitution and another contained ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01420-07
更新日期:2007-12-01 00:00:00
abstract::Prestimulation of the TLR4 pathway with lipopolysaccharide (LPS) protects mice from lethal infection with H5N1 influenza virus. Here, we reveal that the TLR4-TRIF pathway is required for this protective effect by using mice whose TLR4-related molecules were knocked out. Microarray analysis of primary mouse lung cultur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06168-11
更新日期:2012-01-01 00:00:00
abstract::The herpesvirus saimiri (HVS) immediate-early gene product encoded by open reading frame (ORF) 57 shares limited amino acid homology with HSV-1 ICP27 and Epstein-Barr virus BMLF1, both regulatory proteins. The ORF 57 gene has been proposed to be spliced based on the genome sequence, and here we confirm the intron-exon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.857-861.1998
更新日期:1998-01-01 00:00:00
abstract::The sodium dodecyl sulfate (SDS) complex of the major glycoprotein of avian myeloblastosis virus exhibited an anomalously low free electrophoretic mobility compared with those of non-glycosylated protein standards. The apparent molecular weight of the glycoprotein calculated from the relation between log molecular wei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.825-827.1978
更新日期:1978-06-01 00:00:00
abstract::Productive viral infection often depends on the manipulation of the cytoskeleton. Herpesviruses, including rhesus monkey rhadinovirus (RRV) and its close homolog, the oncogenic human gammaherpesvirus Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 (KSHV/HHV8), exploit microtubule (MT)-based retrograde tran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00304-17
更新日期:2017-08-10 00:00:00
abstract::We reported previously that the movement protein (MP) of tomato mosaic tobamovirus is phosphorylated, and we proposed that MP phosphorylation is important for viral pathogenesis. Experimental data indicated that phosphorylation enhances the stability of MP in vivo and enables the protein to assume the correct intracel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1452-1461.2003
更新日期:2003-01-01 00:00:00
abstract::In HIV-1 vaccine research, native-like, soluble envelope glycoprotein SOSIP trimers are widely used for immunizing animals. The epitopes of autologous neutralizing antibodies (NAbs) induced by the BG505 and B41 SOSIP trimers in rabbits and macaques have been mapped to a few holes in the glycan shields that cover most ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01656-18
更新日期:2019-02-05 00:00:00
abstract::Capsids of polyomaviruses--small, nonenveloped DNA viruses--consist of the major structural protein VP1 and the minor structural proteins VP2 and VP3. The contributions of the individual capsid proteins to functions of the viral particle, such as DNA encapsidation, cell receptor attachment, entry, and uncoating, are s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7517-7526.1996
更新日期:1996-11-01 00:00:00
abstract::The nuclear localization of the herpes simplex virus transcriptional activator protein ICP4 was studied by indirect immunofluorescence. At early times after viral infection, ICP4 quickly localized to a diffuse intranuclear distribution. ICP4 later concentrated in globular compartments within the nucleus. The redistrib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.276-284.1987
更新日期:1987-02-01 00:00:00
abstract::Co-infection of neonatal BALB/c mice with Friend virus (FV) complex (containing defective spleen focus-forming virus [SFFV] and endogenous N-tropic leukemia-inducing helper virus [LLV-F]) and B-tropic Tennant leukemia virus (TenLV) resulted in the inhibition of LLV-F by the Fv-1(b) gene and recovery of a TenLV pseudot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.523-533.1973
更新日期:1973-09-01 00:00:00
abstract::Nondefective reticuloendotheliosis virus induces chicken bursal lymphoma in a manner similar to that of avian leukosis virus. The provirus integrates in the c-myc locus and uses a promoter insertion mechanism to activate c-myc expression. We cloned a provirus involved in c-myc activation from a B lymphoma. Detailed st...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2084-2090.1987
更新日期:1987-07-01 00:00:00
abstract:UNLABELLED:Paramyxoviruses and other negative-strand RNA viruses encode matrix proteins that coordinate the virus assembly process. The matrix proteins link the viral glycoproteins and the viral ribonucleoproteins at virus assembly sites and often recruit host machinery that facilitates the budding process. Using a co-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02103-14
更新日期:2014-11-01 00:00:00
abstract::We have used deletion and recombinant long terminal repeat (LTR) mutants to examine enhancer activity differences between LTRs of the nonpathogenic Akv and the thymus lymphomagenic MCF13 murine retroviruses. Deletion mutant analysis revealed that major control regions for MCF13 and Akv LTR enhancer activity were simil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3353-3361.1989
更新日期:1989-08-01 00:00:00
abstract::Rio Bravo virus (RBV) is a member of the family Flaviviridae, genus Flavivirus. It belongs to a group of viruses in the genus with no known vector. In this report, we analyze the complete genome of the prototype RBV, strain M64. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00331-12
更新日期:2012-04-01 00:00:00
abstract::Herpes simplex virus 1 replication initiates angiogenesis and inflammation in the cornea. This can result in herpetic stromal keratitis (HSK), which is a leading cause of infection-induced corneal blindness. Host cellular factors mediate the progression of HSK, but little is known about these cellular factors and thei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-12
更新日期:2012-08-01 00:00:00
abstract::We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05809-11
更新日期:2012-04-01 00:00:00
abstract::Infection of receptor-bearing cells by coronaviruses is mediated by their spike (S) proteins. The coronavirus (SARS-CoV) that causes severe acute respiratory syndrome (SARS) infects cells expressing the receptor angiotensin-converting enzyme 2 (ACE2). Here we show that codon optimization of the SARS-CoV S-protein gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10628-10635.2004
更新日期:2004-10-01 00:00:00
abstract::We report a Sendai virus (SeV) vector system for expression of major histocompatibility complex (MHC) class I/peptide complexes. We cloned the extracellular domain of a human MHC class I heavy chain, HLA-A*2402, and human beta-2 microglobulin (beta2m) fused with HLA-A*2402-restricted human immunodeficiency virus type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.11982-11988.2002
更新日期:2002-12-01 00:00:00
abstract::The adjuvant effects of cytokines in humoral and cell-mediated immunity to herpes simplex virus type 1 (HSV-1) have been examined in mice using HSV-1 recombinant viruses expressing murine interleukin-2 (IL-2), IL-4, or gamma interferon (IFN-gamma) gene. Groups of naive BALB/c mice were immunized intraperitoneally with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5774-5783.2003
更新日期:2003-05-01 00:00:00