Abstract:
:MicroRNAs (miRNAs) are key regulators of gene expression in higher eukaryotes. Recently, miRNAs have been identified from viruses with double-stranded DNA genomes. To attempt to identify miRNAs encoded by herpes simplex virus 1 (HSV-1), we applied a computational method to screen the complete genome of HSV-1 for sequences that adopt an extended stem-loop structure and display a pattern of nucleotide divergence characteristic of known miRNAs. Using this method, we identified 11 HSV-1 genomic loci predicted to encode 13 miRNA precursors and 24 miRNA candidates. Eight of the HSV-1 miRNA candidates were predicted to be conserved in HSV-2. The precursor and the mature form of one HSV-1 miRNA candidate, which is encoded approximately 450 bp upstream of the transcription start site of the latency-associated transcript (LAT), were detected during infection of Vero cells by Northern blot hybridization. These RNAs, which behave as late gene products, are not predicted to be conserved in HSV-2. Additionally, small RNAs, including some that are roughly the expected size of precursor miRNAs, were detected using probes for miRNA candidates derived from sequences encoding the 8.3-kilobase LAT, from sequences complementary to U(L)15 mRNA, and from the region between ICP4 and U(S)1. However, no species the size of typical mature miRNAs were detected using these probes. Three of these latter miRNA candidates were predicted to be conserved in HSV-2. Thus, HSV-1 encodes at least one miRNA. We hypothesize that HSV-1 miRNAs regulate viral and host gene expression.
journal_name
J Viroljournal_title
Journal of virologyauthors
Cui C,Griffiths A,Li G,Silva LM,Kramer MF,Gaasterland T,Wang XJ,Coen DMdoi
10.1128/JVI.00200-06subject
Has Abstractpub_date
2006-06-01 00:00:00pages
5499-508issue
11eissn
0022-538Xissn
1098-5514pii
80/11/5499journal_volume
80pub_type
杂志文章abstract:UNLABELLED:Demonstrating direct interactions between host and virus proteins during infection is a major goal and challenge for the field of virology. Most protein interactions are not binary or easily amenable to structural determination. Using infectious preparations of a polerovirus (Potato leafroll virus [PLRV]) an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01706-15
更新日期:2015-12-09 00:00:00
abstract:UNLABELLED:In a previous screen of putative interferon-stimulated genes, SUN2 was shown to inhibit HIV-1 infection in an uncharacterized manner. SUN2 is an inner nuclear membrane protein belonging to the linker of nucleoskeleton and cytoskeleton complex. We have analyzed here the role of SUN2 in HIV infection. We repor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03202-15
更新日期:2016-03-28 00:00:00
abstract::Escherichia coli K-12 wild type and a uvrA mutant derivative were used to construct isogenic strains bearing one, two, three, or more phage lambda cI genomes and containing increasing concentration of lambda repressor as measured by in vitro operator DNA-binding assays. The survival and phage induction in response to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.595-602.1978
更新日期:1978-06-01 00:00:00
abstract::Influenza A virus NS2 protein, also called nuclear export protein (NEP), is crucial for the nuclear export of viral ribonucleoproteins. However, the molecular mechanisms of NEP mediation in this process remain incompletely understood. A leucine-rich nuclear export signal (NES2) in NEP, located at the predicted N2 heli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06519-11
更新日期:2013-01-01 00:00:00
abstract::The influenza A virus (IAV) M2 protein is a multifunctional protein with critical roles in virion entry, assembly, and budding. M2 is targeted to the apical plasma membrane of polarized epithelial cells, and the interaction of the viral proteins M2, M1, HA, and NA near glycolipid rafts in the apical plasma membrane is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01425-18
更新日期:2018-10-29 00:00:00
abstract::The human antibody response to flavivirus infection is dominantly directed against a cross-reactive epitope on the fusion loop of domain II (DII-FL) of the envelope (E) protein. Although antibodies against this epitope fail to recognize fully mature West Nile virus (WNV) virions and accordingly neutralize infection po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05859-11
更新日期:2011-11-01 00:00:00
abstract::The natural life cycle of alphaviruses, a group of plus-strand RNA viruses, involves transmission to vertebrate hosts via mosquitoes. Chronic infections are established in mosquitoes (and usually in mosquito cell cultures), but infection of susceptible vertebrate cells typically results in rapid shutoff of host mRNA t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.3854-3865.1999
更新日期:1999-05-01 00:00:00
abstract::Ebola virus is the etiologic agent of a lethal hemorrhagic fever in humans and nonhuman primates with mortality rates of up to 90%. Previous studies with Zaire Ebola virus (ZEBOV), mouse-adapted virus (MA-ZEBOV), and mutant viruses (ZEBOV-NP(ma), ZEBOV-VP24(ma), and ZEBOV-NP/VP24(ma)) allowed us to identify the mutati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00659-11
更新日期:2011-09-01 00:00:00
abstract::Growth of bacteriophage T7 is inhibited by the antibiotic coumermycin A1, an inhibitor of the Escherichia coli DNA gyrase. Since growth of the phage is insensitive to the antibiotic in strains containing a coumermycin-resistant DNA gyrase, this enzyme appears to be required for phage growth. We have investigated the e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.529-535.1979
更新日期:1979-02-01 00:00:00
abstract::Latent Epstein-Barr virus (EBV) infection is associated with several lymphoproliferative disorders, including posttransplant lymphoma, Hodgkin's disease, and Burkitt's lymphoma, as well as nasopharyngeal carcinoma (NPC). Twenty-nine microRNAs (miRNAs) have been identified that are transcribed during latent infection f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00785-08
更新日期:2008-09-01 00:00:00
abstract::As potential targets for human immunodeficiency virus type 1 and simian immunodeficiency virus (HIV-1 and SIV), dendritic cells (DCs) likely play a significant role in the onset and spread of infection as well as in the induction of antiviral immunity. Using the SIV-macaque system to study the very early events in DC-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.6.2936-2951.2002
更新日期:2002-03-01 00:00:00
abstract::Herpesviruses are important pathogens of humans and other animals. Herpesvirus infectious clones that can reconstitute phenotypically wild-type (wt) virus are extremely valuable tools for elucidating the roles of specific genes in virus pathophysiology as well as for making vaccines. Ictalurid herpesvirus 1 (channel c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02152-07
更新日期:2008-04-01 00:00:00
abstract::Hepatitis E virus (HEV) is a common cause of acute viral hepatitis worldwide. Most HEV infections are asymptomatic, but immunocompromised patients infected with HEV genotype 3 (HEV3), HEV4, or HEV7 may develop chronic infections. The HEV particles in stools are naked (nHEV), while those in the serum and culture supern...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01207-18
更新日期:2019-02-05 00:00:00
abstract::p60src, the transforming protein of Rous sarcoma virus, was found to contain 0.5 to 0.9 mol of total phosphate per mol of polypeptide. The protein is known to be phosphorylated at two sites, a serine in the amino-terminal domain and a tyrosine in the carboxy-terminal domain. Because our indirect analysis suggests that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.3.813-820.1982
更新日期:1982-03-01 00:00:00
abstract::In the spherical capsid of hepatitis B virus (HBV), intermolecular disulfide bonds cross-link the approximately 180 p21.5 capsid protein subunits into a stable lattice. In this study, we used mutant capsid proteins to investigate the role that disulfide bonds and the four p21.5 Cys residues (positions 48, 61, 107, and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5393-5398.1992
更新日期:1992-09-01 00:00:00
abstract::Purified retrovirus Gag proteins can assemble in vitro into virus-like particles (VLPs) in the presence of RNA. It was shown previously that a Rous sarcoma virus Gag protein missing only the protease domain forms spherical particles resembling immature virions lacking a membrane but that a similar protein missing the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.21.10260-10268.2000
更新日期:2000-11-01 00:00:00
abstract::Epstein-Barr virus (EBV), a member of human gammaherpesvirus, infects mainly B cells. EBV has two alternative life cycles, latent and lytic, and is reactivated occasionally from the latent stage to the lytic cycle. To combat EBV-associated disorders, understanding the molecular mechanisms of the EBV lytic replication ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00975-17
更新日期:2017-11-14 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent for KS tumors, multicentric Castleman's disease, and primary effusion lymphomas. Like other herpesvirus capsids, the KSHV capsid is an icosahedral structure composed of six proteins. The capsid shell is made up of the major capsid protein, two tripl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00423-08
更新日期:2008-07-01 00:00:00
abstract::HA20, a mutant of Bacillus subtilis phage SPO1, is deficient in both DNA replication and late transcription. HA20 contains mutations in two different genes, which suggested that the two effects might be caused independently. However, single-mutation derivatives, affected only in gene 27, were deficient for both activi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.300-301.1984
更新日期:1984-01-01 00:00:00
abstract::How chemokines shape the immune response to viral infection of the central nervous system (CNS) has largely been considered within the context of recruitment and activation of antigen-specific lymphocytes. However, chemokines are expressed early following viral infection, suggesting an important role in coordinating i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.585-594.2004
更新日期:2004-01-01 00:00:00
abstract::Cell-to-cell movement of beet yellows closterovirus requires four structural proteins and a 6-kDa protein (p6) that is a conventional, nonstructural movement protein. Here we demonstrate that either virus infection or p6 overexpression results in association of p6 with the rough endoplasmic reticulum. The p6 protein p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3704-3709.2004
更新日期:2004-04-01 00:00:00
abstract::Merkel cell polyomavirus (MCPyV) is the major cause for Merkel cell carcinoma (MCC), a rare but highly aggressive skin cancer predominantly found in elderly and immunosuppressed patients. The early viral gene products large T-antigen (LT) and small T-antigen (sT) are important for efficient viral DNA replication, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01638-19
更新日期:2020-02-14 00:00:00
abstract:UNLABELLED:The HIV-1 envelope glycoprotein (Env) is a trimer of gp120-gp41 heterodimers and is essential for viral entry. The gp41 subunit in native, prefusion trimeric Env exists in a metastable conformation and attains a stable six-helix bundle (6-HB) conformation comprised of a trimer of N-heptad repeat (NHR) and C-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00494-14
更新日期:2014-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) vpr inhibits the replication of tumor cell lines and peripheral blood mononuclear cells. Here it is demonstrated that expression of vpr, either in the context of a provirus or from an independent genetic element, induces a discrete cell cycle arrest, with cells containing 4N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6859-6864.1995
更新日期:1995-11-01 00:00:00
abstract::The cellular immune response contributes to viral clearance as well as to liver injury in acute and chronic hepatitis C virus (HCV) infection. An immunodominant determinant frequently recognized by liver-infiltrating and circulating CD8(+) T cells of HCV-infected patients is the HCV(NS3-1073) peptide CVNGVCWTV. Using ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11392-11400.2001
更新日期:2001-12-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential protein. Previous studies have shown that the UL25 gene product is a virion component (M. A. Ali et al., Virology 216:278-283, 1996) involved in virus penetration and capsid assembly (C. Addison e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1060-1070.1998
更新日期:1998-02-01 00:00:00
abstract::The rate of transcription in isolated nuclei of primary mouse kidney cells increases by a factor of about 1.5 between 5 and 8 h after infection with polyoma virus or simian virus 40. This process requires intact virus and is inhibited by cycloheximide and actinomycin D. It appears to involve the activity of all three ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.8-19.1980
更新日期:1980-07-01 00:00:00
abstract::After herpes simplex virus infection of hamster kidney cells there is an induction of nucleoside phosphotransferase activity which can utilize AMP as phosphate donor. The activity is immunologically specific for the infected cell and is induced concomitantly with the virus-coded pyrimidine deoxynucleoside kinase activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.1056-1059.1976
更新日期:1976-03-01 00:00:00
abstract::The infection of a bacterium by a phage starts with attachment to a receptor molecule on the host cell surface by the phage. Since receptor-phage interactions are crucial to successful infections, they are major determinants of phage host range and, by extension, of the broader effects that phages have on bacterial co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-20
更新日期:2020-11-09 00:00:00
abstract::Three monkeypox virus (MPV) antibody-secreting murine monoclones were characterized as being of the immunoglobulin G1 isotype, gave a 4+ reaction in the indirect fluorescent-antibody test, gave a positive reaction in the enzyme immunoassay, and did not neutralize MPV. These monoclonal antibodies were determined by the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.1.290-292.1984
更新日期:1984-10-01 00:00:00