Abstract:
:Three monkeypox virus (MPV) antibody-secreting murine monoclones were characterized as being of the immunoglobulin G1 isotype, gave a 4+ reaction in the indirect fluorescent-antibody test, gave a positive reaction in the enzyme immunoassay, and did not neutralize MPV. These monoclonal antibodies were determined by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis transblot method to react to a 15,500-molecular-weight MPV polypeptide. This reactivity could not be removed by adsorption to a vaccinia virus-infected cell suspension. The three monoclonal antibodies were specific for MPV when tested against epidemiologically unrelated isolates of cowpox virus, variola virus, vaccinia virus, and MPV.
journal_name
J Viroljournal_title
Journal of virologyauthors
Roumillat LF,Patton JL,Davis MLdoi
10.1128/JVI.52.1.290-292.1984subject
Has Abstractpub_date
1984-10-01 00:00:00pages
290-2issue
1eissn
0022-538Xissn
1098-5514journal_volume
52pub_type
杂志文章abstract::Coronaviruses are the largest RNA viruses, and their genomes encode replication machinery capable of efficient replication of both positive- and negative-strand viral RNAs as well as enzymes capable of processing large viral polyproteins into putative replication intermediates and mature proteins. A model described re...
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pub_type: 杂志文章
doi:10.1128/JVI.62.2.629-632.1988
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