Abstract:
:Hepatitis B virus (HBV) DNA integration has been shown to occur frequently in human hepatocellular carcinomas. We have investigated whether common cellular DNA domains might be rearranged, possibly by HBV integration, in human primary liver tumors. Unique cellular DNA sequences adjacent to an HBV integration site were isolated from a patient with hepatitis B surface antigen-positive hepatocellular carcinoma. These probes detected rearrangement of this cellular region of chromosomal DNA in 3 of 50 additional primary liver tumors studied. Of these three tumor samples, two contained HBV DNA, without an apparent link between the viral DNA and the rearranged allele; HBV DNA sequences were not detected in the third tumor sample. By use of a panel of somatic cell hybrids, these unique cellular DNA sequences were shown to be located on chromosome 4. Therefore, this region of chromosomal DNA might be implicated in the formation of different tumors at one step of liver cell transformation, possibly related to HBV integration.
journal_name
J Viroljournal_title
Journal of virologyauthors
Pasquinelli C,Garreau F,Bougueleret L,Cariani E,Grzeschik KH,Thiers V,Croissant O,Hadchouel M,Tiollais P,Bréchot Cdoi
10.1128/JVI.62.2.629-632.1988subject
Has Abstractpub_date
1988-02-01 00:00:00pages
629-32issue
2eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and O. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyces cerevisiae plasmids ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.5157-5160.1992
更新日期:1992-08-01 00:00:00
abstract::Novel molecular approaches to inhibit human immunodeficiency virus type 1 (HIV-1) infection have received increasing attention because of the lack of effective antiviral drug therapies in vivo. We now demonstrate that cells can be intracellularly immunized by cytoplasmic expression of single-chain variable antibody fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3392-3400.1996
更新日期:1996-06-01 00:00:00
abstract::The mechanism of lysis by in vivo-induced cytotoxic T lymphocytes (CTL) was examined with virus-specific CTL from mice infected with lymphocytic choriomeningitis virus (LCMV). LCMV-induced T cells were shown to have greater than 10 times the serine esterase activity of T cells from normal mice, and high levels of seri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3726-3733.1990
更新日期:1990-08-01 00:00:00
abstract::The adaptive immune system generates CD8 cytotoxic T lymphocytes (CTLs) as a major component of the protective response against viruses. Knowledge regarding the nature of the peptide sequences presented by HLA class I molecules and recognized by CTLs is thus important for understanding host-pathogen interactions. In t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.24.13399-13408.2004
更新日期:2004-12-01 00:00:00
abstract::We investigated the use of infectious cDNA for the production of poliovirus type 1-type 3 recombinants. One such recombinant virus was produced, but a second construct involving the transfer of part of the capsid protein region was not infectious. Our results suggest that the approach may prove valuable but that not a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.1187-1190.1986
更新日期:1986-03-01 00:00:00
abstract::The expression of reporter genes driven by the same human elongation factor 1alpha (EF1alpha) promoter in murine leukemia virus (MLV)- and human immunodeficiency virus type 1 (HIV-1)-based vectors was studied in either transfected or virally transduced cells. The HIV-1 vectors consistently expressed 3 to 10 times high...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.14.7209-7219.2002
更新日期:2002-07-01 00:00:00
abstract::Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.608-618.1977
更新日期:1977-06-01 00:00:00
abstract::The structural gene for diphtherial toxin is present in corynebacteriophage beta. Previous studies located several point mutations within the tox locus and determined the orientation of transcription of the toxin structural gene. The production of maximal quantities of toxin by Corynebacterium diphtheriae C7(beta) occ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.946-954.1981
更新日期:1981-03-01 00:00:00
abstract::Strains of Bacillus that harbor defective phage PBSX were found to be insensitive to SP-10(C), although the phage adsorbed to these insensitive strains. Strains that did not carry the phage were sensitive to SP-10(C). B. subtilis 168 ind(-), which can be tranduced by SP-10(C) but is nonpermissive for the phage, was re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:
更新日期:1968-08-01 00:00:00
abstract::The cancer-related mucin-type carbohydrate neoantigen Tn was found on gp160 and gp120 of human immunodeficiency virus (HIV). Immunoglobulin G (IgG) and IgM monoclonal antibodies (MAbs) against Tn neutralized infection with cell-free virus and blocked fusion between HIV-infected and uninfected cells. This inhibition wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6461-6467.1991
更新日期:1991-12-01 00:00:00
abstract::Herpesviruses are members of a diverse family of viruses that colonize all vertebrates from fish to mammals. Although more than one hundred herpesviruses exist, all are nearly identical architecturally, with a genome consisting of a linear double-stranded DNA molecule (100 to 225 kbp) protected by an icosahedral capsi...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01169-10
更新日期:2010-12-01 00:00:00
abstract::Rotavirus infection is one of the most common causes of diarrheal illness in humans. In neonatal mice, rhesus rotavirus (RRV) can induce biliary atresia (BA), a disease resulting in inflammatory obstruction of the extrahepatic biliary tract and intrahepatic bile ducts. We previously showed that the amino acid arginine...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00510-17
更新日期:2017-07-12 00:00:00
abstract::The development of resistance to host defense mechanisms such as tumor necrosis factor (TNF)- and Fas-mediated apoptosis of transformed or virus-infected cells may be a critical component in the development of disease. To find genes that protect cells from apoptosis, we used an expression cloning strategy and identifi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7509-7517.1997
更新日期:1997-10-01 00:00:00
abstract::Transcription of herpesviral late genes is stimulated after the onset of viral DNA replication but otherwise restricted. Late gene expression in gammaherpesviruses requires the coordination of six early viral proteins, termed viral transactivation factors (vTFs). Here, we mapped the organization of this protein comple...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02157-15
更新日期:2015-10-14 00:00:00
abstract::The nuclear localization of the herpes simplex virus transcriptional activator protein ICP4 was studied by indirect immunofluorescence. At early times after viral infection, ICP4 quickly localized to a diffuse intranuclear distribution. ICP4 later concentrated in globular compartments within the nucleus. The redistrib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.276-284.1987
更新日期:1987-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02723-07
更新日期:2008-06-01 00:00:00
abstract::Restriction factors are intrinsic cellular defense proteins that have evolved to block microbial infections. Retroviruses such as HIV-1 are restricted by TRIM5 proteins, which recognize the viral capsid shell that surrounds, organizes, and protects the viral genome. TRIM5α uses a SPRY domain to bind capsids with low i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01563-17
更新日期:2018-01-30 00:00:00
abstract::Extrachromosomal DNA purified from mink cells acutely infected with the Snyder-Theilen strain of feline sarcoma virus (FeSV) was digested with restriction endonucleases, and the DNA fragments were electrophoretically separated, transferred to a solid substrate, and hybridized with radiolabeled DNA transcripts compleme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.860-875.1979
更新日期:1979-12-01 00:00:00
abstract::Paramyxoviruses initiate entry through the concerted action of the tetrameric attachment glycoprotein (HN, H, or G) and the trimeric fusion glycoprotein (F). The ectodomains of HN/H/G contain a stalk region important for oligomeric stability and for the F triggering resulting in membrane fusion. Paramyxovirus HN, H, a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00076-12
更新日期:2012-06-01 00:00:00
abstract::Regulatory elements interacting with DNA-binding proteins have been investigated in the promoter sequence of the early PE38 gene in the Autographa californica nuclear polyhedrosis virus (AcNPV). A GATA motif located 50 nucleotides upstream of the PE38 transcriptional start site is recognized differentially in the cour...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3494-3503.1992
更新日期:1992-06-01 00:00:00
abstract::Human keratinocytes immortalized by full-length or early-region simian virus 40 (SV40) DNA grow in agarose and form tumors in nude mice, in contrast to keratinocytes immortalized by the E6/E7 genes of human papillomaviruses. To determine the molecular basis for this biological difference in growth, we have used the in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.10685-10691.2002
更新日期:2002-11-01 00:00:00
abstract::ICP0 is a nuclear phosphoprotein involved in the activation of herpes simplex virus type 1 (HSV-1) gene expression during lytic infection and reactivation from viral latency. Although available evidence suggests that ICP0 acts at the level of transcription, definitive studies specifically addressing this issue have no...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6850-6862.1997
更新日期:1997-09-01 00:00:00
abstract::Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
abstract::Varicella-zoster virus (VZV) is a highly species-specific herpesvirus that targets sensory ganglionic neurons. This species specificity has limited the study of many aspects of VZV pathogenesis, including neuronal infection. We report development of a highly efficient neuroblastoma cell model to study productive VZV i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00515-11
更新日期:2011-08-01 00:00:00
abstract::Reovirus attachment protein σ1 engages glycan receptors and junctional adhesion molecule-A (JAM-A) and is thought to undergo a conformational change during the proteolytic disassembly of virions to infectious subvirion particles (ISVPs) that accompanies cell entry. The σ1 protein is also the primary target of neutrali...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01621-16
更新日期:2017-01-31 00:00:00
abstract::Whole-body bioimaging was used to study dissemination of vaccinia virus (VACV) in normal and in immune deficient (nu(-)/nu(-)) mice protected from lethality by postchallenge administration of ST-246. Total fluxes were recorded in the liver, spleen, lungs, and nasal cavities of live mice after intranasal infection with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03426-12
更新日期:2013-05-01 00:00:00
abstract::We recently found that a human T-cell leukemia virus type 1-infected cell line, MT-2, could support the replication of hepatitis C virus (HCV) (N. Kato, T. Nakazawa, T. Mizutani, and K. Shimotohno, Biochem. Biophys. Res. Commun. 206:863-869, 1995). In order to develop a culture system in which HCV replicates more effi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.7219-7223.1996
更新日期:1996-10-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) is a large (150-kb) double-stranded DNA virus that forms latent infections in neuronal cells of the human peripheral nervous system. Previous work determined that the HSV-1 genome is found in an ordered nucleosomal structure during latent infection. However, during lytic infection, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.10178-10186.2004
更新日期:2004-09-01 00:00:00
abstract::Immunosuppressed patients are at risk for developing Epstein-Barr Virus (EBV)-positive lymphomas that express the major EBV oncoprotein, LMP1. Although increasing evidence suggests that a small number of lytically infected cells may promote EBV-positive lymphomas, the impact of enhanced lytic gene expression on the ab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00770-12
更新日期:2012-08-01 00:00:00
abstract::Poliovirus (PV) is easily transferred to humans orally; however, no rodent model for oral infections has been developed because of the alimentary tract's low sensitivity to the virus. Here we showed that PV is inactivated by the low pH of the gastric contents in mice. The addition of 3% NaHCO3 to the viral inoculum in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02675-06
更新日期:2007-08-01 00:00:00