Abstract:
:Rotavirus infection is one of the most common causes of diarrheal illness in humans. In neonatal mice, rhesus rotavirus (RRV) can induce biliary atresia (BA), a disease resulting in inflammatory obstruction of the extrahepatic biliary tract and intrahepatic bile ducts. We previously showed that the amino acid arginine (R) within the sequence SRL (amino acids 445 to 447) in the RRV VP4 protein is required for viral binding and entry into biliary epithelial cells. To determine if this single amino acid (R) influences the pathogenicity of the virus, we generated a recombinant virus with a single amino acid mutation at this site through a reverse genetics system. We demonstrated that the RRV mutant (RRVVP4-R446G) produced less symptomatology and replicated to lower titers both in vivo and in vitro than those seen with wild-type RRV, with reduced binding in cholangiocytes. Our results demonstrate that a single amino acid change in the RRV VP4 gene influences cholangiocyte tropism and reduces pathogenicity in mice.IMPORTANCE Rotavirus is the leading cause of diarrhea in humans. Rhesus rotavirus (RRV) can also lead to biliary atresia (a neonatal human disease) in mice. We developed a reverse genetics system to create a mutant of RRV (RRVVP4-R446G) with a single amino acid change in the VP4 protein compared to that of wild-type RRV. In vitro, the mutant virus had reduced binding and infectivity in cholangiocytes. In vivo, it produced fewer symptoms and lower mortality in neonatal mice, resulting in an attenuated form of biliary atresia.
journal_name
J Viroljournal_title
Journal of virologyauthors
Mohanty SK,Donnelly B,Dupree P,Lobeck I,Mowery S,Meller J,McNeal M,Tiao Gdoi
10.1128/JVI.00510-17subject
Has Abstractpub_date
2017-07-12 00:00:00issue
15eissn
0022-538Xissn
1098-5514pii
JVI.00510-17journal_volume
91pub_type
杂志文章abstract::Cell-to-cell spread of tobacco mosaic virus is facilitated by the virus-encoded 30-kDa movement protein (MP). This process involves interaction of viral proteins with host components, including the cytoskeleton and the endoplasmic reticulum (ER). During virus infection, high-molecular-weight forms of MP were detected ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.3330-3337.2000
更新日期:2000-04-01 00:00:00
abstract::Scavenger receptor class B type I (SR-BI) is an essential receptor for hepatitis C virus (HCV) and a cell surface high-density-lipoprotein (HDL) receptor. The mechanism of SR-BI-mediated HCV entry, however, is not clearly understood, and the specific protein determinants required for the recognition of the virus envel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02199-08
更新日期:2010-01-01 00:00:00
abstract::We have found that the replicative helicase E1 of bovine papillomavirus type 1 (BPV-1) interacts with a key cell cycle regulator of S phase, the cyclin E-Cdk2 kinase. The E1 helicase, which interacts with cyclin E and not with Cdk2, presents the highest affinity for catalytically active kinase complexes. In addition, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7255-7262.1998
更新日期:1998-09-01 00:00:00
abstract::Recombinant viruses were constructed to have an Escherichia coli replicon containing a mutagenesis marker, the supF gene, integrated within the thymidine kinase locus (tk) of herpes simplex virus type 1. These viruses expressed either wild-type or mutant DNA polymerase (Pol) and were tested in a mutagenesis assay for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3605-3614.2002
更新日期:2002-04-01 00:00:00
abstract::Virus-like particles were purified from stools of patients in an epidemic of hepatitis A in Germany. When reference MS-1 chimpanzee pre-inoculation and convalescent sera were used, the close serological relationship of the purified particles to well-known isolates of hepatitis A could be established. On the other hand...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.1.40-47.1978
更新日期:1978-04-01 00:00:00
abstract::The DNAs of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses contain overlapping segments of the early region of wild-type SV40 DNA. The complementary DNA strands of these five viruses have been separated with synthetic polyribonucleotides in isopycnic cesium chloride gradients. The relat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.677-689.1974
更新日期:1974-03-01 00:00:00
abstract::In response to viral infection, host cells elicit a number of responses, including the expression of alpha/beta interferon (IFN-alpha/beta). In these cells, IFN regulatory factor-3 (IRF-3) undergoes a sequence of posttranslational modifications that allow it to act as a potent transcriptional coactivator of specific I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8411-8420.2004
更新日期:2004-08-01 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV-68) glycoprotein B (gB) was identified in purified virions by immunoblotting, immunoprecipitation, and immunoelectron microscopy. It was synthesized as a 120-kDa precursor in infected cells and cleaved into 65-kDa and 55-kDa disulfide-linked subunits close to the time of virion release....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13370-13375.2004
更新日期:2004-12-01 00:00:00
abstract::Initiation of reverse transcription of human immunodeficiency virus type 1 (HIV-1) occurs by extension from the 3' end of a cellular tRNA complexed to the primer binding site (PBS) located near the 5' end of the viral RNA genome. Although the PBSs for all naturally occurring HIV-1 viruses are complementary to the 3'-t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.207-217.1997
更新日期:1997-01-01 00:00:00
abstract::Resolution of virus infections depends on the priming of virus-specific CD8+ T cells by dendritic cells (DC). While this process requires major histocompatibility complex (MHC) class I-restricted antigen presentation by DC, the relative contribution to CD8+ T cell priming by infected DC is less clear. We have addresse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01508-17
更新日期:2018-01-17 00:00:00
abstract::Infectious cDNA clones of mild (CHV1-Euro7) and severe (CHV1-EP713) hypovirus strains responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus Cryphonectria parasitica were used to construct viable chimeric viruses. Differences in virus-mediated alterations of fungal colony morphology, growt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.16.7562-7567.2000
更新日期:2000-08-01 00:00:00
abstract::The Epstein-Barr virus (EBV) nuclear antigen 3C (EBNA-3C) protein is a transcriptional regulator of viral and cellular genes that is essential for EBV-mediated immortalization of B lymphocytes in vitro. EBNA-3C can inhibit transcription through an association with the cellular DNA-binding protein Jkappa, a function sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5151-5160.2000
更新日期:2000-06-01 00:00:00
abstract::Defining a macrophage-tropic phenotype for HIV-1 to assess a role in pathogenesis is complicated by the fact that HIV-1 isolates vary continuously in their ability to enter monocyte-derived macrophages (MDMs) in vitro, and MDMs vary in their ability to support HIV-1 entry. To overcome these limitations, we identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02477-13
更新日期:2014-02-01 00:00:00
abstract::Besides its role in viral assembly, the vesicular stomatitis virus (VSV) matrix (M) protein causes cytopathic effects such as cell rounding (D. Blondel, G. G. Harmison, and M. Schubert, J. Virol. 64:1716-1725, 1990). DNA cotransfection assays demonstrated that VSV M protein was able to inhibit the transcription of a r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3529-3537.1995
更新日期:1995-06-01 00:00:00
abstract:UNLABELLED:Aminoquinolines and piperazines, linked or not, have been used successfully to treat malaria, and some molecules of this family also exhibit antiviral properties. Here we tested several derivatives of 4-aminoquinolines and piperazines for their activity against hepatitis C virus (HCV). We screened 11 molecul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00404-16
更新日期:2016-09-12 00:00:00
abstract::A panel of monoclonal antibodies that recognize the two major glycoproteins of bovine viral diarrhea virus (BDV) was used to evaluate the antigenic relationship between cytopathic (CP) and noncytopathic (NCP) viruses isolated from cattle dead or dying from fatal BDV infections. Various unrelated BDV isolates were init...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2823-2827.1988
更新日期:1988-08-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) is a DNA virus that acquires an envelope by budding into the inner nuclear membrane of an infected cell. Recombinant HSV-1 lacking the U(L)34 gene cannot undergo this event. U(L)34 and U(L)31, another viral protein, colocalize in an infected cell and are necessary and sufficient to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7601-7610.2003
更新日期:2003-07-01 00:00:00
abstract::Chinese hamster X mouse somatic cell hybrids were analyzed by Southern blot hybridization with a probe specific for the cellular c-fms proto-oncogene. Results demonstrate that Fms, the genetic locus containing this sequence, maps to mouse chromosome 18. Mouse Fms is thus not linked to the same set of genes involved in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.1055-1056.1988
更新日期:1988-03-01 00:00:00
abstract::Rotavirus replication and virulence are strongly influenced by virus strain and host species. The rotavirus proteins VP3, VP4, VP7, NSP1, and NSP4 have all been implicated in strain and species restriction of replication; however, the mechanisms have not been fully determined. Simian (RRV) and bovine (UK) rotaviruses ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02408-10
更新日期:2011-03-01 00:00:00
abstract::The VP6 protein of bluetongue virus possesses a number of activities, including nucleoside triphosphatase, RNA binding, and helicase activity (N. Stauber, J. Martinez-Costas, G. Sutton, K. Monastyrskaya, and P. Roy, J. Virol. 71:7220-7226, 1997). Although the enzymatic functions of the protein have been documented, a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11347-11356.2003
更新日期:2003-11-01 00:00:00
abstract::A region in addition to and outside the long terminal repeats (LTRs) in the gag gene of the Prague A strain of Rous sarcoma virus was found to be essential in cis for efficient cell transformation by cloned viral DNA. Transformation in chicken embryo fibroblasts, which requires infectious virus production and reinfect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.11.3401-3409.1987
更新日期:1987-11-01 00:00:00
abstract::Production of Sindbis virus in the presence of transcription and translation inhibitors was examined in three Aedes albopictus cell lines. Addition of cycloheximide to heat-resistant Sindbis virus (SVHR)-infected mosquito cells arrested viral RNA synthesis completely, in contrast to the effects of this drug on virus-i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2629-2635.1988
更新日期:1988-08-01 00:00:00
abstract::The bacteriophage T4 unf gene, known to be involved in the arrest of transcription from cytosine-containing DNA, is unessential except in Escherichia coli strains containing plasmid pR386. Comparative genetic and biochemical analyses of parameters of unf+ and unf- phage growth in host cells isogenic except for the pre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.430-439.1985
更新日期:1985-02-01 00:00:00
abstract::We used the mammalian reoviruses to determine the molecular basis of the clearance of a virus from the bloodstream by specific organs. Reovirus serotypes 1 (T1) and 3 (T3) were radiolabeled with [35S]methionine or 125I, and the viruses were injected intravenously into weanling rats. The distribution of radioactivity w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.545-551.1988
更新日期:1988-02-01 00:00:00
abstract::The major adenovirus (Ad) capsid proteins hexon, penton, and fiber influence the efficiency and tropism of gene transduction by Ad vectors. Fiber is the high-affinity receptor binding protein that serves to mediate cell attachment in vitro when using coxsackie-adenovirus receptor (CAR)-containing cell lines. This cont...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11627-11637.2005
更新日期:2005-09-01 00:00:00
abstract::Seasonal influenza viruses are a major cause of human disease worldwide. Most neutralizing antibodies (Abs) elicited by influenza viruses target the head domain of the hemagglutinin (HA) protein. Anti-HA head Abs can be highly potent, but they have limited breadth since the HA head is variable. There is great interest...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1128/JVI.02134-18
更新日期:2019-04-03 00:00:00
abstract::The efficiency and specificity of gene transfer with human adenovirus (hAd)-derived gene transfer vectors would be improved if the native viral tropism could be modified. Here, we demonstrate that the minor capsid protein IX (pIX), which is present in 240 copies in the Ad capsid, can be exploited as an anchor for hete...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3470-3479.2004
更新日期:2004-04-01 00:00:00
abstract::The E7 proteins of human papillomaviruses (HPVs) promote S-phase reentry in differentiated keratinocytes of the squamous epithelia to support viral DNA amplification. In this study, we showed that nuclear p130 was present in the differentiated strata of several native squamous epithelia susceptible to HPV infection. I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01202-07
更新日期:2008-05-01 00:00:00
abstract::Influenza causes significant morbidity in tropical regions; however, unlike in temperate zones, influenza in the tropics is not strongly associated with a given season. We have recently shown that influenza virus transmission in the guinea pig model is most efficient under cold, dry conditions, which are rare in the t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00325-08
更新日期:2008-06-01 00:00:00
abstract::The genome of a human papillomavirus (HPV) found in a patient with Bowen's disease of the skin was molecularly cloned. Blot hybridization experiments, performed under stringent conditions, revealed no cross-hybridization between this HPV DNA and the other known HPV DNAs, showing that this HPV represents a new type, te...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.2.688-692.1986
更新日期:1986-02-01 00:00:00