Abstract:
:Virus-like particles were purified from stools of patients in an epidemic of hepatitis A in Germany. When reference MS-1 chimpanzee pre-inoculation and convalescent sera were used, the close serological relationship of the purified particles to well-known isolates of hepatitis A could be established. On the other hand, the physicochemical characteristics of the particles were determined in parallel to the characteristics of a marker parvovirus (LuIII) and a marker picornavirus (poliovirus type 2). It could be shown that the majority of the hepatitis A-associated particles band at 1.34 g/ml in CsCl and, like poliovirus, sediment at about 160S. In addition, a distinct hepatitis A antigen was observed, which banded at 1.305 g/ml and sedimented between 50 and 90S. A further component accumulated in the density range of between 1.38 and 1.44 g/ml. However, it seemed to be rather labile. Upon reisolation from CsCl and sedimentation in sucrose, it resolved into a 160S, a 90 to 100S, and a 50S form. The size of the 160S particles (27 to 29 nm) could be readily distinguished from that of the parvovirus (22 to 24 nm). It is concluded, therefore, that hepatitis A-associated virus particles are more likely to be classified with the picornaviruses than with the parvoviruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Siegl G,Frösner GGdoi
10.1128/JVI.26.1.40-47.1978subject
Has Abstractpub_date
1978-04-01 00:00:00pages
40-7issue
1eissn
0022-538Xissn
1098-5514journal_volume
26pub_type
杂志文章abstract::A recombinant capripoxvirus vaccine containing a cDNA of the peste-des-petits-ruminants virus (PPRV) fusion protein gene was constructed. A quick and efficient method was used to select a highly purified recombinant virus clone. A trial showed that a dose of this recombinant as low as 0.1 PFU protected goats against c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1571-1577.2003
更新日期:2003-01-01 00:00:00
abstract::The V1V2 region of HIV-1 gp120 harbors a major vulnerable site targeted by a group of broadly neutralizing monoclonal antibodies (MAbs) such as PG9 through strand-strand recognition. However, this epitope region is structurally polymorphic as it can also form a helical conformation recognized by RV144 vaccine-induced ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01409-16
更新日期:2016-11-28 00:00:00
abstract::Glycoprotein H (gH) is conserved among all herpesviruses and is essential for virus entry and cell fusion along with gL, gB, and, in most alphaherpesviruses, gD. Within the gH/gL heterodimer, it is thought that gH accounts for the fusion function and gL acts as a chaperone for the folding and transport of gH. Here, we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00097-07
更新日期:2007-05-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a cluster of 12 microRNAs (miRNAs) that are processed from a transcript that is embedded within the major latency control region. We have generated a deletion mutation that eliminates 10 of the 12 viral miRNAs from the KSHV bacmid by using recombineering methods. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-09
更新日期:2010-03-01 00:00:00
abstract::During the late phase of simian virus 40 infections of CV1 cells, the relative ratios of the spliced to the unspliced RNA molecules of the 19S family were measured. In the cytoplasm, unspliced 19S RNA represented between 1 and 2% of spliced 19S RNA. This ratio could be altered by the use of different methods of RNA ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.595-600.1980
更新日期:1980-11-01 00:00:00
abstract::Using 3'-end-labeled genome probes, cells infected with vesicular stomatitis virus Chandipura, Cocal, and Piry serotypes were shown to contain (+) leader RNAs of approximately 50 nucleotides in length. The nucleotide sequence of the leader RNA regions of these genomes was determined and compared with the previously re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.125-130.1983
更新日期:1983-04-01 00:00:00
abstract::Bacteriophages of the C3 morphotype, characterized by very long heads that exceed their width several times, are extremely rare among the Podoviridae family members and constitute only 0.5% of over 5,500 phages that have been examined by the electron microscope (H. W. Ackermann, Arch. Virol. 152:227-243, 2007; H. W. A...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02727-12
更新日期:2012-12-01 00:00:00
abstract::Cellular inhibitor of apoptosis protein 2 (cIAP2) is a potent suppressor of apoptotic cell death. We have shown previously that cIAP2 is involved in the tumor necrosis factor alpha (TNF-α)-induced anti-hepatitis B virus (HBV) response; however, the mechanism for this antiviral effect remains unclear. In the present st...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00879-11
更新日期:2011-11-01 00:00:00
abstract::Normal levels of the hypermodified pyrimidine, alpha-putrescinylthymine, which is formed from hydhydroxymethyluracil at the polynucleotide level (Maltman et al., J. Virol. 34:354-359, 1984), are not required in bacteriophage luminal diameterW-14 DNA for the DNA to serve as a replicative template in luminal diameterW-1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1036-1038.1984
更新日期:1984-12-01 00:00:00
abstract::Human cytomegalovirus (HCMV) virions are composed of a DNA-containing nucleocapsid surrounded by a tegument layer and host-derived lipid envelope studded with virally encoded glycoproteins. These complex virions are estimated to be composed of more than 50 viral proteins. Assembly of HCMV virions is poorly understood,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01551-10
更新日期:2011-01-01 00:00:00
abstract::Poliovirus-infected cells undergo an increase in cytoplasmic calcium concentrations from the 4th h postinfection. The protein responsible for this effect was identified by the expression of different poliovirus nonstructural proteins in HeLa cells by using a recombinant vaccinia virus system. Synthesis of protein 2BC ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.6214-6217.1997
更新日期:1997-08-01 00:00:00
abstract::Hepatitis C virus (HCV)-specific CD8 cell exhaustion may represent a mechanism of HCV persistence. The inhibitory receptor PD-1 has been reported to be up-regulated in exhausted CD8 cells. Therefore, we studied PD-1 expression longitudinally during acute HCV infection. Most HCV-specific CD8 cells expressed PD-1 at the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01177-06
更新日期:2006-11-01 00:00:00
abstract::Experimental reverse genetic replacement of Epstein-Barr virus nuclear antigen 3A (EBNA3A) with a conditional mutant EBNA3A revealed that EBNA3A is critical for continued lymphoblastoid cell (LCL) growth. Wild-type (wt) EBNA3A expressed in the LCLs specifically sustained growth under nonpermissive conditions, whereas ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.16.10171-10179.2005
更新日期:2005-08-01 00:00:00
abstract::We used 18 monoclonal antibodies against B19 parvovirus to identify neutralizing epitopes on the viral capsid. Of the 18 antibodies, 9 had in vitro neutralizing activity in a bone marrow colony culture assay. The overlapping polypeptide fragments spanning the B19 structural proteins were produced in a pMAL-c Escherich...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.7056-7060.1991
更新日期:1991-12-01 00:00:00
abstract::The membrane-associated alphavirus RNA replication complex contains four virus-encoded subunits, the nonstructural proteins nsP1 to nsP4. Semliki Forest virus (SFV) nsP1 is hydrophobically modified by palmitoylation of cysteines 418 to 420. Here we show that Sindbis virus nsP1 is also palmitoylated on the same site (c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.6725-6733.2000
更新日期:2000-08-01 00:00:00
abstract::Newcastle disease virus (NDV) California strain reportedly grows poorly in L cells but replicates very well in chicken embryo cells. NDV-infected L cell cultures show a characteristic virus growth curve with respect to uridine incorporation, but plaque assays of the virus produced 24 h postinfection (PI) show no infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.162-169.1974
更新日期:1974-07-01 00:00:00
abstract::An effective vaccine for AIDS may require development of novel vectors capable of eliciting long-lasting immune responses. Here we report the development and use of replication-competent and replication-defective strains of recombinant herpes simplex virus (HSV) that express envelope and Nef antigens of simian immunod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7745-7754.2000
更新日期:2000-09-01 00:00:00
abstract::Previous studies in our laboratory showed that the RNA debranching enzyme (DBR1) is not required for early steps in HIV cDNA formation but is necessary for synthesis of intermediate and late cDNA products. To further characterize this effect, we evaluated the topology of the 5' end of the HIV-1 RNA genome during early...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01377-17
更新日期:2017-11-14 00:00:00
abstract::The protease (PR) from human immunodeficiency virus (HIV) is essential for viral replication: this aspartyl protease, active only as a dimer, is responsible for cleavage of the viral polyprotein precursors (Gag and Gag-Pol), to release the functional mature proteins. In this work, we have studied the structure-functio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8216-8226.2003
更新日期:2003-08-01 00:00:00
abstract::Spumaviruses, or foamy viruses, express Gag proteins that are incompletely processed by the viral protease in cell cultures. To delineate the proteolytic cleavage sites between potential Gag subdomains, recombinant human spumaretrovirus (HSRV) Gag proteins of different lengths were expressed, purified by affinity chro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7907-7911.1999
更新日期:1999-09-01 00:00:00
abstract::Alpha/beta interferons (IFN-alpha/beta) are key mediators of innate immunity and important modulators of adaptive immunity. The mechanisms by which IFN-alpha/beta are induced are becoming increasingly well understood. Recent studies showed that Toll-like receptors 7 and 8 expressed by plasmacytoid dendritic cells (pDC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.16.10376-10385.2005
更新日期:2005-08-01 00:00:00
abstract::Viral particles purified from species of the protozoan parasite Leishmania braziliensis subsp. guyanensis by centrifugation in CsCl gradients were examined for the presence of viral polymerase. We demonstrated that RNA-dependent RNA polymerase is associated with viral particles. Viral transcription was studied in vitr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3712-3715.1990
更新日期:1990-08-01 00:00:00
abstract:UNLABELLED:Influenza B virus causes annual epidemics and, along with influenza A virus, accounts for substantial disease and economic burden throughout the world. Influenza B virus infects only humans and some marine mammals and is not responsible for pandemics, possibly due to a very low frequency of reassortment and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01576-15
更新日期:2015-12-01 00:00:00
abstract::Induction of virus-specific T-cell responses in mucosal as well as systemic compartments of the immune system is likely to be a critical feature of an effective AIDS vaccine. We investigated whether virus-specific CD8(+) lymphocytes induced in rhesus macaques by immunization with attenuated simian immunodeficiency vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8762-8766.2000
更新日期:2000-09-01 00:00:00
abstract::The cell surface receptor T cell immunoglobulin mucin domain 1 (TIM-1) dramatically enhances filovirus infection of epithelial cells. Here, we showed that key phosphatidylserine (PtdSer) binding residues of the TIM-1 IgV domain are critical for Ebola virus (EBOV) entry through direct interaction with PtdSer on the vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01025-13
更新日期:2013-08-01 00:00:00
abstract::After herpes simplex virus infection of hamster kidney cells there is an induction of nucleoside phosphotransferase activity which can utilize AMP as phosphate donor. The activity is immunologically specific for the infected cell and is induced concomitantly with the virus-coded pyrimidine deoxynucleoside kinase activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.1056-1059.1976
更新日期:1976-03-01 00:00:00
abstract::Partial deletion of the second hypervariable region from the envelope of the primary-like SF162 virus increases the exposure of certain neutralization epitopes and renders the virus, SF162DeltaV2, highly susceptible to neutralization by clade B and non-clade B human immunodeficiency virus (HIV-positive) sera (L. Stama...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5526-5540.2001
更新日期:2001-06-01 00:00:00
abstract::The p21 proteins of ras oncogenes are synthesized as precursors in the cytosol. After processing, which involves acylation, the products are associated with the plasma membrane in eucaryotic cells. The p21 overproduced in Escherichia coli, however, is not processed by acylation. A synthetic tetrapeptide of the p21 C t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.607-612.1985
更新日期:1985-11-01 00:00:00
abstract::The human cytomegalovirus UL47 open reading frame encodes a 110-kDa protein that is a component of the virion tegument. We have constructed a cytomegalovirus mutant, ADsubUL47, in which the central portion of the UL47 open reading frame has been replaced by two marker genes. The mutant replicated to titers 100-fold lo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1043-1050.2002
更新日期:2002-02-01 00:00:00
abstract::Candidate foot-and-mouth disease (FMD) DNA vaccines designed to produce viral capsids lacking infectious viral nucleic acid were evaluated. Plasmid DNAs containing a portion of the FMDV genome coding for the capsid precursor protein (P1-2A) and wild-type or mutant viral proteinase 3C (plasmids P12X3C or P12X3C-mut, re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4454-4457.1998
更新日期:1998-05-01 00:00:00