Abstract:
:Cellular inhibitor of apoptosis protein 2 (cIAP2) is a potent suppressor of apoptotic cell death. We have shown previously that cIAP2 is involved in the tumor necrosis factor alpha (TNF-α)-induced anti-hepatitis B virus (HBV) response; however, the mechanism for this antiviral effect remains unclear. In the present study, we demonstrate that cIAP2 can significantly reduce the levels of HBV DNA replication intermediates but not the total viral RNA or core protein levels. Domain-mapping analysis revealed that the carboxy-terminal domains of cIAP2 were indispensable for this anti-HBV ability and that an E3 ligase-deficient mutant of cIAP2 (termed cIAP2*) completely lost its antiviral activity. We further identified HBV polymerase as the target of cIAP2. Overexpression of cIAP2 but not cIAP2* reduced polymerase protein levels, while cIAP2 knockdown increased polymerase expression. In addition, we observed that cIAP2 promoted the degradation of the viral polymerase through a proteasome-dependent pathway. Further experiments demonstrated that cIAP2 can bind to polymerase and promote its polyubiquitylation. Finally, we found that cIAP2 downregulated the encapsidation of HBV pregenomic RNA. Taken together, these data reveal a novel mechanism for the inhibition of HBV replication by cIAP2 via acceleration of the ubiquitin-proteasome-mediated decay of polymerase and reduction of the encapsidation of HBV pregenomic RNA, making this mechanism a novel strategy for HBV therapy.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wang Z,Ni J,Li J,Shi B,Xu Y,Yuan Zdoi
10.1128/JVI.00879-11subject
Has Abstractpub_date
2011-11-01 00:00:00pages
11457-67issue
21eissn
0022-538Xissn
1098-5514pii
JVI.00879-11journal_volume
85pub_type
杂志文章abstract::The architecture of a retroviral genome presents some unusual features for transcriptional regulation because of duplication of the transcriptional control sequences in the 5' and 3' long terminal repeats (LTRs). We have studied the transcriptional activity of the 5' and 3' LTRs of human immunodeficiency virus type 1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3830-3840.1994
更新日期:1994-06-01 00:00:00
abstract::Artificial target sequences for tissue-specific miRNAs have recently been introduced as a new means for altering the tissue tropism of viral replication. This approach can be used to improve the safety of oncolytic viruses for cancer virotherapy by restricting their replication in unwanted tissues, such as the liver. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01940-12
更新日期:2013-01-01 00:00:00
abstract::Ocular herpes simplex keratitis (HSK) is a consequence of viral reactivations from trigeminal ganglia (TG) and occurs almost exclusively in the same eye in humans. In our murine oro-ocular (OO) model, herpes simplex virus 1 (HSV-1) inoculation in one side of the lip propagates virus to infect the ipsilateral TG. Repli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01586-19
更新日期:2019-11-26 00:00:00
abstract::Hepatitis C virus (HCV) enters cells via a pH- and clathrin-dependent endocytic pathway. Scavenger receptor BI (SR-BI) and CD81 are important entry factors for HCV internalization into target cells. The SR-BI gene gives rise to at least two mRNA splice variants, SR-BI and SR-BII, which differ in their C termini. SR-BI...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02356-06
更新日期:2007-04-01 00:00:00
abstract::Oncostatin M (OSM) is released together with type I interferon (IFN) by activated dendritic cells, suggesting a concerted action of these cytokines in the biological response against infection. We found that OSM increases the antiviral effect of IFN-alpha in Huh7 hepatoma cells infected with hepatitis A or hepatitis C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02167-08
更新日期:2009-04-01 00:00:00
abstract::By marker rescue with cloned herpes simplex virus 2 DNA fragments, we have mapped the temperature-sensitive mutations of a series of herpes simplex virus 2 mutants to a region of the herpes simplex virus 2 genome that lies within or near the coding sequences for the major DNA-binding protein, ICP8. In cells infected w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.332-342.1983
更新日期:1983-01-01 00:00:00
abstract::A virus (M-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus RD-114. Immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between M-7 and RD-114. Anti-RD-114 DNA polymerase IgG inhibits M-7 polymerase by 57% compared to 97% for RD-114. M-7 vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.133-138.1974
更新日期:1974-07-01 00:00:00
abstract::Chinese hamster ovary (CHO) cells have recently been used for identification of receptors for several alphaherpesviruses, including pseudorabies virus (PrV) (R. J. Geraghty, C. Krummenacher, G. H. Cohen, R. J. Eisenberg, and P. G. Spear, Science 280:1618-1620, 1998). The experiments were based on the fact that CHO cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8019-8026.1999
更新日期:1999-10-01 00:00:00
abstract::The stimulation of host macromolecular synthesis and induction into the cell cycle of serum-deprived G0-G1-arrested mouse embryo fibroblasts were examined after infection of resting cells with wild-type simian virus 40 or with viral mutants affecting T antigen (tsA58) or small t antigen (dl884). At various times after...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.2.802-812.1981
更新日期:1981-02-01 00:00:00
abstract::Three late transcripts have been mapped to the vaccinia virus 7.1-kilobase (kb) EcoRI F fragment, which is located approximately 85 kb from the left end of the viral genome. Hybrid selection and translation of RNA have demonstrated that these mRNAs encode three polypeptides with apparent molecular weights of 32,000 (3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.510-520.1984
更新日期:1984-02-01 00:00:00
abstract:UNLABELLED:Respiratory syncytial virus (RSV) is the most important cause of lower respiratory tract infection in young children and the leading cause of infant hospitalization worldwide. Uncontrolled response to RSV is mediated by a toll-like receptor (TLR)-mediated immune response. Resveratrol possesses anti-RSV activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03637-13
更新日期:2014-04-01 00:00:00
abstract::The distantly related lentiviruses human immunodeficiency virus type 1 (HIV-1) and visna virus each encode a posttranscriptional regulatory protein, termed Rev, that is critical for expression of the viral structural proteins. We genetically mapped the cis-acting target sequence for visna virus Rev, the visna virus Re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3609-3615.1992
更新日期:1992-06-01 00:00:00
abstract::The morphogenesis of vesicular stomatitis virus was examined using freeze-fracture techniques, and the results obtained were compared with those from previously published experiments carried out with influenza viruses and togaviruses. The process of conversion of the host cell plasma membrane into the vesicular stomat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.601-609.1977
更新日期:1977-02-01 00:00:00
abstract::The Epstein-Barr virus nuclear antigen 2 (EBNA-2) acidic domain is essential for B-lymphocyte growth transformation and can activate transcription when brought to a promoter by a sequence-specific DNA-binding domain. We now show that the EBNA-2 acidic domain has slightly less activity than the proteotypic acidic trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.585-588.1995
更新日期:1995-01-01 00:00:00
abstract::An unusually long noncoding sequence is located between the N gene of Borna disease virus (BDV) and the genes for regulatory factor X and polymerase cofactor P. Most of these nucleotides are transcribed and seem to control translation of the bicistronic X/P mRNA. We report here that Vero cells persistently infected wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00639-08
更新日期:2008-10-01 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) replication in dendritic cells (DCs) is restricted by SAMHD1. This factor is counteracted by the viral protein Vpx; Vpx is found in HIV-2 and simian immunodeficiency virus (SIV) from sooty mangabeys (SIVsm) or from macaques (SIVmac) but is absent from HIV-1. We pre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03057-13
更新日期:2014-05-01 00:00:00
abstract::Mitogenic activities of simian virus 40 large T and small t antigens were studied in serum-deprived human diploid fibroblasts. Wild-type large T and small t cooperated in stimulating DNA synthesis and in inducing hyperphosphorylation of the Rb gene product (pRb). In contrast, a T antigen mutant defective for pRb bindi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3724-3732.1994
更新日期:1994-06-01 00:00:00
abstract::The herpes simplex virus (HSV) capsid is released into the cytoplasm after fusion of viral and host membranes, whereupon dynein-dependent trafficking along microtubules targets it to the nuclear envelope. Binding of the capsid to the nuclear pore complex (NPC) is mediated by the capsid protein pUL25 and the capsid-tet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00641-17
更新日期:2017-07-12 00:00:00
abstract::Pigs are permissive to both human and avian influenza viruses and have been proposed to be an intermediate host for the genesis of pandemic influenza viruses through reassortment or adaptation of avian viruses. Prospective virological surveillance carried out between March 1998 and June 2000 in Hong Kong, Special Admi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9679-9686.2001
更新日期:2001-10-01 00:00:00
abstract::Dengue virus (DENV) and hepatitis C virus (HCV), members of the family Flaviviridae, are global human health concerns. As positive-strand RNA viruses, they each replicate in the cytoplasm of infected cells and induce distinct membranous replication compartments where most, if not all, steps of the viral life cycle occ...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.03404-13
更新日期:2014-06-01 00:00:00
abstract::RNA silencing is a conserved eukaryotic gene regulatory system in which sequence specificity is determined by small RNAs. Plant RNA silencing also acts as an antiviral mechanism; therefore, viral infection requires expression of a silencing suppressor. The mechanism and the evolution of silencing suppression are still...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.7217-7226.2005
更新日期:2005-06-01 00:00:00
abstract::Polyomavirus large T antigen is phosphorylated on both serine and threonine residues at a ratio of approximately 6 to 1. This phosphorylation could be resolved into a series of nine Staphylococcus aureus V8 phosphopeptides. All of these were found in an N-terminal chymotryptic fragment with a molecular weight of 57,00...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.1155-1163.1987
更新日期:1987-04-01 00:00:00
abstract::In 2002, severe acute respiratory syndrome (SARS)-coronavirus (CoV) appeared as a novel human virus with high similarity to bat coronaviruses. However, while SARS-CoV uses the human angiotensin-converting enzyme 2 (ACE2) receptor for cellular entry, no coronavirus isolated from bats appears to use ACE2. Here we show t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00311-12
更新日期:2012-06-01 00:00:00
abstract::Several arenaviruses, chiefly Lassa virus (LASV) and Junin virus in West Africa and Argentina, respectively, cause hemorrhagic fever (HF) disease in humans that is associated with high morbidity and significant mortality. The investigation of antiviral strategies to combat HF arenaviruses is hampered by the requiremen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02229-10
更新日期:2011-02-01 00:00:00
abstract::To date, no vaccine that is safe and effective against herpes simplex virus 2 (HSV-2) disease has been licensed. In this study, we evaluated a DNA prime-formalin-inactivated-HSV-2 (FI-HSV2) boost vaccine approach in the guinea pig model of acute and recurrent HSV-2 genital disease. Five groups of guinea pigs were immu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02521-10
更新日期:2011-04-01 00:00:00
abstract:UNLABELLED:The immunoglobulin superfamily protein receptors for poliovirus, human rhinovirus, and coxsackievirus B (CVB) serve to bind the viruses to target cells and to facilitate the release of the virus genome by catalyzing the transition from the mature infectious virus to the A-particle uncoating intermediate. Rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01790-14
更新日期:2014-10-01 00:00:00
abstract::RmI, a circular chimera made of the polyomavirus (Py) genome with an insertion of mouse DNA (Ins), effectively undergoes intramolecular recombination in normal mouse cells, as indicated by the conversion of cloned RmI (RmIc) into unit-length Py DNA in transfected cultures. To follow the fate of the cellular component ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.3.845-850.1987
更新日期:1987-03-01 00:00:00
abstract::Influenza A viruses of the H9N2 subtype are endemic in poultry in many Eurasian countries and have occasionally caused clinical respiratory diseases in humans. While some avian H9N2 viruses have glutamine (Q) at amino acid position 226 of the hemagglutinin (HA) receptor-binding site, an increasing number of isolates h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02827-06
更新日期:2007-05-01 00:00:00
abstract::To determine which sequences or structures in the poliovirus 5' noncoding region (5'NCR) are involved in binding proteins used for internal ribosome binding and protein synthesis initiation, translation competition assays were performed in rabbit reticulocyte lysates in the presence and absence of HeLa cell extract. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.3101-3109.1992
更新日期:1992-05-01 00:00:00
abstract:UNLABELLED:In vitro, infection of polarized human intestinal epithelial cells by coxsackievirus B3 (CVB3) depends on virus interaction with decay-accelerating factor (DAF), a receptor expressed on the apical cell surface. Although mice are highly susceptible to CVB3 infection when virus is delivered by intraperitoneal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03468-14
更新日期:2015-04-01 00:00:00