Use of single-cycle infectious lymphocytic choriomeningitis virus to study hemorrhagic fever arenaviruses.


:Several arenaviruses, chiefly Lassa virus (LASV) and Junin virus in West Africa and Argentina, respectively, cause hemorrhagic fever (HF) disease in humans that is associated with high morbidity and significant mortality. The investigation of antiviral strategies to combat HF arenaviruses is hampered by the requirement of biosafety level 4 (BSL-4) facilities to work with these viruses. These biosafety hurdles could be overcome by the use of recombinant single-cycle infectious arenaviruses. To explore this concept, we have developed a recombinant lymphocytic choriomeningitis virus (LCMV) (rLCMVΔGP/GFP) where we replaced the viral glycoprotein (GP) with the green fluorescent protein (GFP). We generated high titers of GP-pseudotyped rLCMVΔGP/GFP via genetic trans complementation using stable cell lines that constitutively express LCMV or LASV GPs. Replication of these GP-pseudotyped rLCMVΔGP/GFP viruses was restricted to GP-expressing cell lines. This system allowed us to rapidly and reliably characterize and quantify the neutralization activities of serum antibodies against LCMV and LASV within a BSL-2 facility. The sensitivity of the GFP-based microneutralization assay we developed was similar to that obtained with a conventionally used focus reduction neutralization (FRNT) assay. Using GP-pseudotyped rLCMVΔGP/GFP, we have also obtained evidence supporting the feasibility of this approach to identify and evaluate candidate antiviral drugs against HF arenaviruses without the need of BSL-4 laboratories.


J Virol


Journal of virology


Rodrigo WW,de la Torre JC,Martínez-Sobrido L




Has Abstract


2011-02-01 00:00:00














  • Analysis of murine hepatitis virus strain A59 temperature-sensitive mutant TS-LA6 suggests that nsp10 plays a critical role in polyprotein processing.

    abstract::Coronaviruses are the largest RNA viruses, and their genomes encode replication machinery capable of efficient replication of both positive- and negative-strand viral RNAs as well as enzymes capable of processing large viral polyproteins into putative replication intermediates and mature proteins. A model described re...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Donaldson EF,Graham RL,Sims AC,Denison MR,Baric RS

    更新日期:2007-07-01 00:00:00

  • A novel cyanophage with a cyanobacterial nonbleaching protein A gene in the genome.

    abstract::A cyanophage, PaV-LD, has been isolated from harmful filamentous cyanobacterium Planktothrix agardhii in Lake Donghu, a shallow freshwater lake in China. Here, we present the cyanophage's genomic organization and major structural proteins. The genome is a 95,299-bp-long, linear double-stranded DNA and contains 142 pot...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gao EB,Gui JF,Zhang QY

    更新日期:2012-01-01 00:00:00

  • Base composition and hybridization studies of the three double-stranded RNA segments of bacteriophage phi 6.

    abstract::The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Van Etten JL,Vidaver AK,Koski RK,Burnett JP

    更新日期:1974-06-01 00:00:00

  • Expression of the Marek's disease virus (MDV) homolog of glycoprotein B of herpes simplex virus by a recombinant baculovirus and its identification as the B antigen (gp100, gp60, gp49) of MDV.

    abstract::A gene encoding a homolog of glycoprotein B of herpes simplex virus (gB homolog) has been identified on the Marek's disease virus (MDV) genome (L. J. N. Ross, M. Sanderson, S. D. Scott, M. M. Binns, T. Doel, and B. Milne, J. Gen. Virol. 70:1789-1804, 1989); however, the molecular and immunological characteristics of t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Niikura M,Matsuura Y,Endoh D,Onuma M,Mikami T

    更新日期:1992-05-01 00:00:00

  • Effect of water-based microencapsulation on protection against EDIM rotavirus challenge in mice.

    abstract::We determined the capacity of microcapsules formed by the combination of sodium alginate, an aqueous anionic polymer, and spermine hydrochloride, an aqueous cationic amine, to enhance protection against rotavirus challenge in mice. Adult BALB/c mice were orally inoculated with either free or microencapsulated rotaviru...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Moser CA,Speaker TJ,Offit PA

    更新日期:1998-05-01 00:00:00

  • Identification of a sequence within U5 required for human immunodeficiency virus type 1 to stably maintain a primer binding site complementary to tRNA(Met).

    abstract::Initiation of reverse transcription of human immunodeficiency virus type 1 (HIV-1) occurs by extension from the 3' end of a cellular tRNA complexed to the primer binding site (PBS) located near the 5' end of the viral RNA genome. Although the PBSs for all naturally occurring HIV-1 viruses are complementary to the 3'-t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kang SM,Zhang Z,Morrow CD

    更新日期:1997-01-01 00:00:00

  • Constitutive phosphorylation of the vesicular stomatitis virus P protein modulates polymerase complex formation but is not essential for transcription or replication.

    abstract::As a subunit of both the P-L polymerase complex and the P-N assembly complex, the vesicular stomatitis virus (VSV) P protein plays a pivotal role in transcription and replication of the viral genome. Constitutive phosphorylation of this protein is currently thought to be essential for formation of the P-L complex. We ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Spadafora D,Canter DM,Jackson RL,Perrault J

    更新日期:1996-07-01 00:00:00

  • MyD88-dependent immune activation mediated by human immunodeficiency virus type 1-encoded Toll-like receptor ligands.

    abstract::Immune activation is a major characteristic of human immunodeficiency virus type 1 (HIV-1) infection and a strong prognostic factor for HIV-1 disease progression. The underlying mechanisms leading to immune activation in viremic HIV-1 infection, however, are not fully understood. Here we show that, following the initi...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Meier A,Alter G,Frahm N,Sidhu H,Li B,Bagchi A,Teigen N,Streeck H,Stellbrink HJ,Hellman J,van Lunzen J,Altfeld M

    更新日期:2007-08-01 00:00:00

  • Purification and chemistry of bacteriophage chi.

    abstract::From a stock of varkappa phage grown on Salmonella, a host-range mutant which attacks Escherichia coli was isolated. As in the case of Salmonella, only motile strains of E. coli are sensitive to varkappa. The phage shows an eclipse period of 35 min and a minimal latent period of 52 min. The adsorption rate constant is...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Schade S,Adler J

    更新日期:1967-06-01 00:00:00

  • E5 oncoprotein transmembrane mutants dissociate fibroblast transforming activity from 16-kilodalton protein binding and platelet-derived growth factor receptor binding and phosphorylation.

    abstract::The E5 oncoprotein of bovine papillomavirus type 1 is a 44-amino-acid, hydrophobic polypeptide which localizes predominantly in Golgi membranes and appears to transform cells through the activation of tyrosine kinase growth factor receptors. In fibroblasts, E5 interacts with both the 16-kilodalton vacuolar ATPase subu...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Sparkowski J,Mense M,Anders J,Schlegel R

    更新日期:1996-04-01 00:00:00

  • Human immunodeficiency virus type 1 is trapped by acidic but not by neutralized human cervicovaginal mucus.

    abstract::To reliably infect a primate model for human immunodeficiency virus (HIV), approximately 10,000-fold more virus must be delivered vaginally than intravenously. However, the vaginal mechanisms that help protect against HIV are poorly understood. Here, we report that human cervicovaginal mucus (CVM), obtained from donor...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lai SK,Hida K,Shukair S,Wang YY,Figueiredo A,Cone R,Hope TJ,Hanes J

    更新日期:2009-11-01 00:00:00

  • Blocking antibody access to neutralizing domains on glycoproteins involved in entry as a novel mechanism of immune evasion by herpes simplex virus type 1 glycoproteins C and E.

    abstract::Herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) blocks complement activation, and glycoprotein E (gE) interferes with IgG Fc-mediated activities. While evaluating gC- and gE-mediated immune evasion in human immunodeficiency virus (HIV)-HSV-1-coinfected subjects, we noted that antibody alone was more effective ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hook LM,Huang J,Jiang M,Hodinka R,Friedman HM

    更新日期:2008-07-01 00:00:00

  • A 348-base-pair region in the latency-associated transcript facilitates herpes simplex virus type 1 reactivation.

    abstract::Latency-associated transcript (LAT) promoter deletion mutants of herpes simplex virus type 1 have a reduced capacity to reactivate following adrenergic induction in the rabbit eye model. We have mapped a reactivation phenotype within LAT and describe the construction of recombinants in which poly(A) addition sites hav...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bloom DC,Hill JM,Devi-Rao G,Wagner EK,Feldman LT,Stevens JG

    更新日期:1996-04-01 00:00:00

  • Transcriptional mapping of rabies virus in vivo.

    abstract::Synthesis of the proteins of rabies virus was studied in hamster cells infected with UV-irradiated virus. The UV target size of genes L, N, M1, and M2 was measured during primary transcription. Except for N, the target size of the remaining genes was considerably larger than that of their physical sizes. The data fit ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Flamand A,Delagneau JF

    更新日期:1978-11-01 00:00:00

  • Characterization of two novel YY1 binding sites in the polyomavirus late promoter.

    abstract::NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, delta, NF-E1, UCRBP, or CF1. Moreover, we show t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Martelli F,Iacobini C,Caruso M,Felsani A

    更新日期:1996-03-01 00:00:00

  • Severe acute respiratory syndrome coronavirus protein 6 accelerates murine hepatitis virus infections by more than one mechanism.

    abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) encodes numerous accessory proteins whose importance in the natural infection process is currently unclear. One of these accessory proteins is set apart by its function in the context of a related murine hepatitis virus (MHV) infection. SARS-CoV protein 6 in...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hussain S,Perlman S,Gallagher TM

    更新日期:2008-07-01 00:00:00

  • The ocr+ gene function of bacteriophages T3 and T7 counteracts the Salmonella typhimurium DNA restriction systems SA and SB.

    abstract::In host cells containing the Salmonella typhimurium DNA restriction-modification systems SA(+) and SB(+), replication of the ocr(+) bacteriophages T3 and T7 is not impaired. However, ocr (gene 0.3) mutants of these phages are susceptible to DNA restriction and modification by the SA(+) and SB(+) systems. ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Krüger DH,Hansen S,Reuter M

    更新日期:1983-03-01 00:00:00

  • A human cellular protein activity (OF-1), which binds herpes simplex virus type 1 origin, contains the Ku70/Ku80 heterodimer.

    abstract::In an effort to identify host proteins involved in herpes simplex virus type 1 replication, monkey and human cellular protein activities (called OF-1) that bind the viral replication origin, oriS, have been described. We show by mass spectrometry that the DNA-binding component of human OF-1 contains Ku70 and Ku80 prot...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Murata LB,Dodson MS,Hall JD

    更新日期:2004-07-01 00:00:00

  • The murine CAR homolog is a receptor for coxsackie B viruses and adenoviruses.

    abstract::Complementary DNA clones encoding the murine homolog (mCAR) of the human coxsackievirus and adenovirus receptor (CAR) were isolated. Nonpermissive CHO cells transfected with mCAR cDNA became susceptible to infection by coxsackieviruses B3 and B4 and showed increased susceptibility to adenovirus-mediated gene transfer....

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bergelson JM,Krithivas A,Celi L,Droguett G,Horwitz MS,Wickham T,Crowell RL,Finberg RW

    更新日期:1998-01-01 00:00:00

  • Enhancement of antiviral activity of human alpha-defensin 5 against herpes simplex virus 2 by arginine mutagenesis at adaptive evolution sites.

    abstract::Herpes simplex virus 2 (HSV-2) infection is still one of the common causes of sexually transmitted diseases worldwide. The prevalence of HSV strains resistant to traditional nucleoside antiviral agents has led to the development of novel antiviral drugs. Human alpha-defensin 5 (HD5), a kind of endogenous antimicrobial...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wang A,Chen F,Wang Y,Shen M,Xu Y,Hu J,Wang S,Geng F,Wang C,Ran X,Su Y,Cheng T,Wang J

    更新日期:2013-03-01 00:00:00

  • The Antagonism of HIV-1 Nef to SERINC5 Particle Infectivity Restriction Involves the Counteraction of Virion-Associated Pools of the Restriction Factor.

    abstract::SERINC3 (serine incorporator 3) and SERINC5 are recently identified host cell inhibitors of HIV-1 particle infectivity that are counteracted by the viral pathogenesis factor Nef. Here we confirm that HIV-1 Nef, but not HIV-1 Vpu, antagonizes the particle infectivity restriction of SERINC5. SERINC5 antagonism occurred ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Trautz B,Pierini V,Wombacher R,Stolp B,Chase AJ,Pizzato M,Fackler OT

    更新日期:2016-11-14 00:00:00

  • Intranasal delivery of recombinant parvovirus-like particles elicits cytotoxic T-cell and neutralizing antibody responses.

    abstract::We previously demonstrated that chimeric porcine parvovirus-like particles (PPV:VLP) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong CD4(+) and CD8(+) T-cell responses specific for the foreign epitopes. In the present study, we investigated the immunogenicity...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Sedlik C,Dridi A,Deriaud E,Saron MF,Rueda P,Sarraseca J,Casal JI,Leclerc C

    更新日期:1999-04-01 00:00:00

  • Construction of recombinant murine retroviruses that express the human T-cell leukemia virus type II and human T-cell lymphotropic virus type III trans activator genes.

    abstract::Recombinant retroviruses containing the trans activator genes of human T-cell leukemia virus (HTLV) type II and human T-cell lymphotropic virus type III were constructed. The trans activator genes tat II and tat III were inserted into the murine retroviral vector pZIPNEOSV(X)1. Recombinant plasmids were transfected in...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rosen CA,Sodroski JG,Campbell K,Haseltine WA

    更新日期:1986-01-01 00:00:00

  • Oligomerization of hepatitis C virus core protein is crucial for interaction with the cytoplasmic domain of E1 envelope protein.

    abstract::Hepatitis C virus (HCV) contains two membrane-associated envelope glycoproteins, E1 and E2, which assemble as a heterodimer in the endoplasmic reticulum (ER). In this study, predictive algorithms and genetic analyses of deletion mutants and glycosylation site variants of the E1 glycoprotein were used to suggest that t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Nakai K,Okamoto T,Kimura-Someya T,Ishii K,Lim CK,Tani H,Matsuo E,Abe T,Mori Y,Suzuki T,Miyamura T,Nunberg JH,Moriishi K,Matsuura Y

    更新日期:2006-11-01 00:00:00

  • Implication of a central cysteine residue and the HHCC domain of Moloney murine leukemia virus integrase protein in functional multimerization.

    abstract::Moloney murine leukemia virus (M-MuLV) IN-IN protein interactions important for catalysis of strand transfer and unimolecular and bimolecular disintegration reactions were investigated by using a panel of chemically modified M-MuLV IN proteins. Functional complementation of an HHCC-deleted protein (Ndelta105) by an in...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Donzella GA,Leon O,Roth MJ

    更新日期:1998-02-01 00:00:00

  • Acidic pH Mediates Changes in Antigenic and Oligomeric Conformation of Herpes Simplex Virus gB and Is a Determinant of Cell-Specific Entry.

    abstract::Herpes simplex virus (HSV) is an important human pathogen with a high worldwide seroprevalence. HSV enters epithelial cells, the primary site of infection, by a low-pH pathway. HSV glycoprotein B (gB) undergoes low pH-induced conformational changes, which are thought to drive membrane fusion. When neutralized back to ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Weed DJ,Dollery SJ,Komala Sari T,Nicola AV

    更新日期:2018-08-16 00:00:00

  • Transcriptional activation and repression by cellular DNA-binding protein C/EBP.

    abstract::A putative transcription factor, C/EBP, isolated from rat liver nuclei, has been shown to bind to at least two different sequence motifs: the CCAAT promoter domain and a core sequence [GTGG(T/A)(T/A)(T/A)G] common to many viral enhancers, including simian virus 40 and human hepatitis B virus. It has been proposed that...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pei DQ,Shih CH

    更新日期:1990-04-01 00:00:00

  • CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry.

    abstract::Hepatitis C virus (HCV) is an enveloped positive-stranded RNA hepatotropic virus. HCV pseudoparticles infect liver-derived cells, supporting a model in which liver-specific molecules define HCV internalization. Three host cell molecules have been reported to be important entry factors or receptors for HCV internalizat...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Harris HJ,Farquhar MJ,Mee CJ,Davis C,Reynolds GM,Jennings A,Hu K,Yuan F,Deng H,Hubscher SG,Han JH,Balfe P,McKeating JA

    更新日期:2008-05-01 00:00:00

  • Electron microscopy of vesicular stomatitis virus replicative ribonucleoproteins.

    abstract::The objective of this investigation was to examine by electron microscopy the replicative ribonucleoprotein (RNP) structures synthesized in vesicular stomatitis virus-infected HeLa cells. Pulse-labeled in vivo products of vesicular stomatitis virus replication and transcription can be separated by centrifugation in Re...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Naeve CW,Summers DF

    更新日期:1980-06-01 00:00:00

  • A survey of the interactome of Kaposi's sarcoma-associated herpesvirus ORF45 revealed its binding to viral ORF33 and cellular USP7, resulting in stabilization of ORF33 that is required for production of progeny viruses.

    abstract:UNLABELLED:The ORF45 protein of Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus-specific immediate-early tegument protein. Our previous studies have revealed its crucial roles in both early and late stages of KSHV infection. In this study, we surveyed the interactome of ORF45 using a panel of monoc...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gillen J,Li W,Liang Q,Avey D,Wu J,Wu F,Myoung J,Zhu F

    更新日期:2015-05-01 00:00:00