Abstract:
:We previously reported that the adenovirus type 5 E3 14.5-kilodalton protein (14.5K) forms a complex with E3 10.4K and that both proteins are required to down-regulate the epidermal growth factor receptor in adenovirus-infected human cells. Both proteins are also required to prevent cytolysis by tumor necrosis factor of most mouse cell lines infected by adenovirus mutants that lack E3 14.7K. The E3 14.5K amino acid sequence suggests that 14.5K is an integral membrane protein with an N-terminal signal sequence for membrane insertion. Here we show that 14.5K was found exclusively in cytoplasmic membrane fractions. Radiochemical sequencing of 14.5K indicated that the N-terminal signal sequence is cleaved predominantly between Cys-18 and Ser-19. With a mutant that does not express 10.4K, cleavage occurs predominantly between Phe-17 and Cys-18, indicating that the presence or absence of 10.4K affects the signal cleavage site. 14.5K was extracted into the detergent phase with Triton X-114, it remained associated with membranes after extraction with Na2CO3 at pH 11.5, and it was partially protected by membranes from proteinase K digestion; these observations indicate that 14.5K is an integral membrane protein. Proteinase K digestion followed by immunoprecipitation with antipeptide antisera directed against the N or C terminus of mature 14.5K indicated that 14.5K is oriented in the membrane with its N terminus in the lumen and its C terminus in the cytoplasm. Thus, 14.5K is a type I bitopic membrane protein. Previous studies indicated that 10.4K is also an integral membrane protein oriented with its C terminus in the cytoplasm. Altogether, these findings suggest that cytoplasmic membranes are the site of action when 10.4K and 14.5K down-regulate the epidermal growth factor receptor and prevent tumor necrosis factor cytolysis.
journal_name
J Viroljournal_title
Journal of virologyauthors
Krajcsi P,Tollefson AE,Anderson CW,Wold WSdoi
10.1128/JVI.66.3.1665-1673.1992subject
Has Abstractpub_date
1992-03-01 00:00:00pages
1665-73issue
3eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::The envelope protein E of flaviviruses mediates both receptor-binding and membrane fusion. At the virion surface, 180 copies of E are tightly packed and organized in a herringbone-like icosahedral structure, whereas in noninfectious subviral particles, 60 copies are arranged in a T=1 icosahedral symmetry. In both case...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00660-09
更新日期:2009-09-01 00:00:00
abstract::The avian adenovirus CELO is a promising vector for gene transfer applications. In order to study this potentiality, we developed an improved method for construction of adenovirus vectors in cosmids that was used to engineer the CELO genome. For all the recombinant viruses constructed by this method, the ability to pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5288-5301.2001
更新日期:2001-06-01 00:00:00
abstract::The retroviral Gag precursor plays an important role in the assembly of virion particles. The capsid (CA) protein of the Gag molecule makes a major contribution to this process. In the crystal structure of the free CA protein of the human immunodeficiency virus type 1 (HIV-1), 11 residues of the C terminus were found ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9675-9688.2004
更新日期:2004-09-01 00:00:00
abstract::YY1 is a zinc finger transcription factor which acts as either a repressor or an activator dependent on the promoter context. YY1 is a potent activator of the genuine human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) in HeLa cells, which are known for high-level expression of the HPV-18 early gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.1-12.1995
更新日期:1995-01-01 00:00:00
abstract::The N-terminal domain of the bovine papillomavirus type 1 E2 protein is important for viral DNA replication, for transcriptional transactivation, and for interaction with the E1 protein. To determine which residues of this 200-amino-acid domain are important for these activities, single conservative amino acid substit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.23-29.1996
更新日期:1996-01-01 00:00:00
abstract::The human genome contains more than half a million human endogenous retrovirus (HERV) long terminal repeats (LTRs) that can be regarded as mobile regulatory modules. Many of these HERV LTRs have been recruited during evolution as transcriptional control elements for cellular gene expression. We have cloned LTR sequenc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00858-09
更新日期:2009-12-01 00:00:00
abstract::The promoter of the murine thymidine kinase gene contains a binding site for transcription factor E2F. Using cell lines (3T3-LT) conditionally expressing polyomavirus large T antigen from a hormone-responsive promoter and reporter gene constructs carrying the thymidine kinase promoter with intact or mutated E2F sites,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.1765-1771.1993
更新日期:1993-04-01 00:00:00
abstract::Functions of viral proteins can be regulated through phosphorylation by serine/threonine kinases in plants, but little is known about the involvement of tyrosine kinases in plant virus infection. In this study, TGBp3, one of the three movement proteins encoded by a triple gene block (TGB) of Potato mop-top virus (PMTV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03388-12
更新日期:2013-04-01 00:00:00
abstract::Major histocompatibility complex E (MHC-E) is a highly conserved nonclassical MHC-Ib molecule that tightly binds peptides derived from leader sequences of classical MHC-Ia molecules for presentation to natural killer cells. However, MHC-E also binds diverse foreign and neoplastic self-peptide antigens for presentation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00592-19
更新日期:2019-09-12 00:00:00
abstract::A method to study the function of individual African swine fever virus (ASFV) gene products utilizing the Escherichia coli lac repressor-operator system has been developed. Recombinant viruses containing both the lacI gene encoding the lac repressor and a strong virus late promoter modified by the insertion of one or ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3185-3195.1998
更新日期:1998-04-01 00:00:00
abstract::In an attempt to generate broadly cross-reactive, neutralizing monoclonal antibodies (MAbs) to simian immunodeficiency virus (SIV), we compared two immunization protocols using different preparations of oligomeric SIV envelope (Env) glycoproteins. In the first protocol, mice were immunized with soluble gp140 (sgp140) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7922-7935.2000
更新日期:2000-09-01 00:00:00
abstract::Interferon (IFN)-stimulated gene factor 2 (ISGF2) plays a role in transcription of the beta IFN (IFN-beta) gene and IFN-stimulated genes (ISGs) and may function as a central mediator of cytokine responses. Constitutive ISGF2 transgene expression resulted in substantial resistance to three RNA virus families. This phen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4470-4478.1992
更新日期:1992-07-01 00:00:00
abstract::A retrovirus vector has been developed that selects for instances in which the provirus integrates in close proximity to cellular promoters. Coding sequences for a selectable marker (histidinol dehydrogenase) were inserted into the 3' long terminal repeat (LTR) of an enhancerless Molony murine leukemia virus. Although...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3227-3233.1989
更新日期:1989-08-01 00:00:00
abstract::Infection of cells with herpes simplex virus type 1 (HSV-1) results in a rapid alteration of phosphorylation on the large subunit of cellular RNA polymerase II (RNAP II), most likely on its C-terminal domain (S. A. Rice, M. C. Long, V. Lam, C. A. Spencer, J. Virol. 68:988-1001, 1994). This phosphorylation modification...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5550-5559.1995
更新日期:1995-09-01 00:00:00
abstract::Duck embryo fibroblast (DEF) and chicken embryo fibroblast (CEF) cultures infected with Marek's disease virus were studied by combined fluorescent antibody and electron microscopy techniques. In both DEF and CEF cultures, cells containing immunofluorescent (IF) antigen also contained herpesvirus particles; conversely,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.1.79-90.1970
更新日期:1970-01-01 00:00:00
abstract:UNLABELLED:Marburg virus (MARV), which belongs to the virus family Filoviridae, causes hemorrhagic fever in humans and nonhuman primates that is often fatal. MARV is a lipid-enveloped virus that during the replication process extracts its lipid coat from the plasma membrane of the host cell it infects. MARV carries sev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02607-15
更新日期:2015-12-30 00:00:00
abstract::Studies on deoxyribonucleic acid purified from the granulosis virus of Trichoplusia ni have revealed the presence of a closed, double-stranded superhelix which sediments at 95S relative to relaxed circles (74S) and linear (60S) forms. Molecular weight estimates show that this insect virus deoxyribonucleic acid has a s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.710-713.1972
更新日期:1972-04-01 00:00:00
abstract::Two murine leukemia viruses were isolated from JLS-V9 cells which had been infected with Rauscher plasma virus. One virus was XC positive and failed to grow on mink or cat cells and thus was an ecotropic virus. The other virus formed cytopathic foci on mink cells, was XC negative, and fell into the mink cell focus-for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.3.995-1003.1983
更新日期:1983-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a lymphotrophic herpesvirus infecting most of the world's population. It is associated with a number of human lymphoid and epithelial tumors and lymphoproliferative diseases in immunocompromised patients. Recent studies have shown an in vitro and in vivo interaction between the EBV nuclear ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.17.8702-8709.2002
更新日期:2002-09-01 00:00:00
abstract::Resistance to human immunodeficiency virus type 1 protease (HIV PR) inhibitors results primarily from the selection of multiple mutations in the protease region. Because many of these mutations are selected for the ability to decrease inhibitor binding in the active site, they also affect substrate binding and potenti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1359-1368.2002
更新日期:2002-02-01 00:00:00
abstract::The initiation of RNA transcription by the virion-bound RNA transcriptase of vesicular stomatitis virus has been examined. Multiple initiation sequences have been observed, two of which have been characterized (pppApCpGp... and pppGpCp...) suggestive of a transcription process which can start at different sites along ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.4.487-501.1973
更新日期:1973-04-01 00:00:00
abstract:UNLABELLED:HIV-1-specific CD8 T cells can influence HIV-1 disease progression during untreated HIV-1 infection, but the functional and phenotypic properties of HIV-1-specific CD8 T cells in individuals treated with suppressive antiretroviral therapy remain less well understood. Here we show that a subgroup of HIV-1-spe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00789-15
更新日期:2015-08-01 00:00:00
abstract::Epstein-Barr virus (EBV) is associated with about 6 to 16% of gastric carcinoma cases worldwide. Expression of the EBV microRNAs (miRNAs) was observed in B cells and nasopharyngeal carcinoma cells infected with EBV. However, it is not clear if the EBV miRNAs are expressed in EBV-associated gastric carcinomas (EBVaGCs)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02271-06
更新日期:2007-01-01 00:00:00
abstract::We report on the use of spectral map analysis of the inter- and intraclade neutralization data of 14 sera of human immunodeficiency virus type 1 (HIV-1)-infected individuals and 16 primary isolates, representing genetic clades A to H in group M and group O. This multivariate analysis has been used previously to study ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6235-6243.1996
更新日期:1996-09-01 00:00:00
abstract::All positive-strand RNA viruses reorganize host intracellular membranes to assemble their replication complexes. Similarly, brome mosaic virus (BMV) induces two alternate forms of membrane-bound RNA replication complexes: vesicular spherules and stacks of appressed double-membrane layers. The mechanisms by which these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06701-11
更新日期:2012-05-01 00:00:00
abstract::The Moloney sarcoma virus-specific onc gene, referred to as v-mos, was used as probe to hybridize to restricted DNAs from various mouse-Chinese hamster hybrid cell lines. These hybrid cells contain, in addition to all of the Chinese hamster chromosomes, various numbers (less than a full complement) of mouse chromosome...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.752-754.1982
更新日期:1982-11-01 00:00:00
abstract::Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective (Rep-) Q beta phage pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2435-2442.1992
更新日期:1992-04-01 00:00:00
abstract::Recognition of viruses by germ line-encoded pattern recognition receptors of the innate immune system is essential for rapid production of type I interferon (IFN) and early antiviral defense. We investigated the mechanisms of viral recognition governing production of type I IFN during herpes simplex virus (HSV) infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01167-07
更新日期:2007-12-01 00:00:00
abstract::A majority of species B adenoviruses (Ads) use CD46 as their primary receptor; however, the precise mechanisms involved in the binding of different Ad types to CD46 have not been resolved. Although previous studies indicate close similarities between two members of species B2 Ads in their usage of CD46, our current in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00754-08
更新日期:2008-08-01 00:00:00
abstract::Adenovirus class B (Ad3 and Ad7) and class C (Ad1, Ad5, and Ad6) late r-strand mRNA's were found to have segmented 5' leaders. These leaders were very similar among serotype within a class but differed in sequence from the leaders on late mRNA's of a different class. However, the leader components of class B viruses m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.3.899-912.1979
更新日期:1979-06-01 00:00:00