Initiation and direction of RNA transcription by vesicular stomatitis virus virion transcriptase.

abstract：:Geminiviruses package circular single-stranded DNA and replicate in the nucleus via a double-stranded intermediate. This intermediate also serves as a template for bidirectional transcription by polymerase II. Here, we map promoters and transcripts and characterize regulatory proteins of Mungbean yellow mosaic virus-V...

journal_title：Journal of virology

authors： Shivaprasad PV,Akbergenov R,Trinks D,Rajeswaran R,Veluthambi K,Hohn T,Pooggin MM

更新日期：2005-07-01 00:00:00

abstract：:Growth of bacteriophage T7 is inhibited by the antibiotic coumermycin A1, an inhibitor of the Escherichia coli DNA gyrase. Since growth of the phage is insensitive to the antibiotic in strains containing a coumermycin-resistant DNA gyrase, this enzyme appears to be required for phage growth. We have investigated the e...

journal_title：Journal of virology

authors： De Wyngaert M,Hinkle DC

更新日期：1979-02-01 00:00:00

abstract：:We have recently shown that live attenuated virus vaccines produced on chicken-derived cells contain low levels of particle-associated reverse transcriptase (RT). In both virus and corresponding control harvests produced on chicken embryo fibroblasts, these activities were present at significantly higher concentration...

journal_title：Journal of virology

authors： Weissmahr RN,Schüpbach J,Böni J

更新日期：1997-04-01 00:00:00

abstract：:Endogenous retroviruses, records of past retroviral infections, are ubiquitous in vertebrate genomes. On occasion, vertebrate hosts have co-opted retroviral genes for their own biological functions. Here, we perform a phylogenomic survey of retroviral gag gene homologs within vertebrate genomes and identify two ancien...

journal_title：Journal of virology

authors： Wang J,Gong Z,Han GZ

更新日期：2019-06-28 00:00:00

abstract：:Infection of cells with enveloped viruses is accomplished through membrane fusion. The binding and fusion processes are mediated by the spike proteins in the envelope of the virus particle and usually involve a series of conformational changes in these proteins. We have studied the low-pH-mediated fusion process of th...

journal_title：Journal of virology

authors： Wahlberg JM,Bron R,Wilschut J,Garoff H

更新日期：1992-12-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) infection requires cell surface expression of CD4. Costimulation of CD8(+)/CD4(-) T lymphocytes by anti-CD3 and anti-CD28 antibodies or by allogeneic dendritic cells induced expression of CD4 and rendered these CD8 cells susceptible to HIV-1 infection. Naive CD45RA+ cells re...

journal_title：Journal of virology

authors： Kitchen SG,Korin YD,Roth MD,Landay A,Zack JA

更新日期：1998-11-01 00:00:00

abstract：:The nucleotide and deduced amino acid sequences of the P1 region of the genomes of 10 independent equine rhinitis A virus (ERAV) isolates were determined and found to be very closely related. A panel of seven monoclonal antibodies to the prototype virus ERAV.393/76 that bound to nonneutralization epitopes conserved am...

journal_title：Journal of virology

authors： Varrasso A,Drummer HE,Huang JA,Stevenson RA,Ficorilli N,Studdert MJ,Hartley CA

更新日期：2001-11-01 00:00:00

abstract：:The current therapy for hepatitis B and C is based on systemic administration of recombinant human alpha interferon (r-hIFN-alpha). However, systemic delivery of r-hIFN-alpha is associated with severe side effects, but more importantly, it is effective in only a small percentage of patients. In an effort to maximize I...

journal_title：Journal of virology

authors： Aurisicchio L,Delmastro P,Salucci V,Paz OG,Rovere P,Ciliberto G,La Monica N,Palombo F

更新日期：2000-05-01 00:00:00

abstract：:Epstein-Barr virus (EBV) undergoes latent and lytic replication cycles, and its reactivation from latency to lytic replication is initiated by expression of the two viral immediate-early transactivators, Zta and Rta. In vitro, reactivation of EBV can be induced by anti-immunoglobulin, tetradecanoyl phorbol acetate, an...

journal_title：Journal of virology

authors： Tsai PF,Lin SJ,Weng PL,Tsai SC,Lin JH,Chou YC,Tsai CH

更新日期：2011-03-01 00:00:00

abstract：:The cloned murine cytolytic T-lymphocyte line IE1-IL and several sublines detect a murine cytomegalovirus immediate-early (IE) membrane determinant in conjunction with Ld class I major histocompatibility glycoprotein. The lines retained cytolytic activity, strict antigen specificity, and self-restriction even when ada...

journal_title：Journal of virology

authors： Reddehase MJ,Fibi MR,Keil GM,Koszinowski UH

更新日期：1986-12-01 00:00:00

abstract：:Treatment with sodium dodecyl sulfate (SDS) converted the vaccinia virus strain IHD-J into particles of two types: (i) ghosts which possessed a thin-membrane vesicle derived from basement part of the virus membrane with attached lateral bodies and a membranous structure derived from the core wall and (ii) aggregates o...

journal_title：Journal of virology

authors： Ichihashi Y,Oie M,Tsuruhara T

更新日期：1984-06-01 00:00:00

abstract：:Novel molecular approaches to inhibit human immunodeficiency virus type 1 (HIV-1) infection have received increasing attention because of the lack of effective antiviral drug therapies in vivo. We now demonstrate that cells can be intracellularly immunized by cytoplasmic expression of single-chain variable antibody fr...

journal_title：Journal of virology

authors： Shaheen F,Duan L,Zhu M,Bagasra O,Pomerantz RJ

更新日期：1996-06-01 00:00:00

abstract：:Due to increasing concerns about human infection by various H7 influenza viruses, including recent H7N9 viruses, we evaluated the genetic relationships and cross-protective efficacies of three different Eurasian H7 avian influenza viruses. Phylogenic and molecular analyses revealed that recent Eurasian H7 viruses can ...

journal_title：Journal of virology

authors： Kwon HI,Kim YI,Park SJ,Song MS,Kim EH,Kim SM,Si YJ,Lee IW,Song BM,Lee YJ,Yun SJ,Kim WJ,Choi YK

更新日期：2017-05-12 00:00:00

abstract：:The high rate of HIV-1 evolution contributes to immune escape, enables the virus to escape drug therapy, and may underlie the difficulty of producing an effective vaccine. Identifying constraints on HIV evolution is therefore of prime importance. To investigate this problem, we examined the relationships between seque...

journal_title：Journal of virology

authors： Woo J,Robertson DL,Lovell SC

更新日期：2010-12-01 00:00:00

abstract：:Individuals infected with the human immunodeficiency virus (HIV) experience a marked loss of CD4+ T lymphocytes, leading to fatal immunodeficiency. The mechanisms causing the depletion of these cells are not yet understood. In this study, we observed that CD4+ T lymphocytes from HIV type 1 (HIV-1)-infected and uninfec...

journal_title：Journal of virology

authors： Heinkelein M,Sopper S,Jassoy C

更新日期：1995-11-01 00:00:00

abstract：:Expression of the cellular heat shock protein 70 gene (hsp70) is transiently induced by human cytomegalovirus (HCMV) infection of permissive human diploid fibroblasts. Induction of the cellular heat shock response during critical times of infection had previously been reported to alter the growth of HCMV in vitro. Thu...

journal_title：Journal of virology

authors： Santomenna LD,Colberg-Poley AM

更新日期：1990-05-01 00:00:00

abstract：:Immunity to human immunodeficiency virus virion-like structures or a polyprotein has been examined after DNA immunization with Rev-independent expression vectors. A Gag-Pol fusion protein stimulated cytotoxic T lymphocyte and antibody responses to Gag and Pol, while a Gag-Pol pseudoparticle did not elicit substantial ...

journal_title：Journal of virology

authors： Huang Y,Kong WP,Nabel GJ

更新日期：2001-05-01 00:00:00

abstract：:Axl, a plasma membrane-associated Tyro3/Axl/Mer (TAM) family member, is necessary for optimal Zaire ebolavirus (ZEBOV) glycoprotein (GP)-dependent entry into some permissive cells but not others. To date, the role of Axl in virion entry is unknown. The focus of this study was to characterize entry pathways that are us...

journal_title：Journal of virology

authors： Hunt CL,Kolokoltsov AA,Davey RA,Maury W

更新日期：2011-01-01 00:00:00

abstract：:Measles virus (MV) isolated in B95a cells, a marmoset B-cell line, retains full pathogenicity for cynomolgus monkeys, while its derivative obtained by adaptation to the growth in Vero cells, a monkey kidney cell line, loses the pathogenic potential (F. Kobune, H. Sakata, and A. Sugiura, J. Virol. 64:700-705, 1990). He...

journal_title：Journal of virology

authors： Takeda M,Kato A,Kobune F,Sakata H,Li Y,Shioda T,Sakai Y,Asakawa M,Nagai Y

更新日期：1998-11-01 00:00:00

abstract：:We have identified a novel brain-derived single-stranded-DNA-binding protein that interacts with a region of the human neurotropic JC virus enhancer designated the lytic control element (LCE). This nuclear factor, LCP-1 (for lytic control element-binding protein 1), specifically recognizes the LCE, as determined by ge...

journal_title：Journal of virology

authors： Tada H,Khalili K

更新日期：1992-12-01 00:00:00

abstract：:The hepadnavirus reverse transcriptase is a multifunction enzyme. In addition to its role in DNA synthesis, the polymerase is required for RNA packaging and also functions as the primer for minus-strand DNA synthesis. Previously, we demonstrated that the protein-priming activity of the polymerase requires a viral RNA ...

journal_title：Journal of virology

authors： Wang GH,Zoulim F,Leber EH,Kitson J,Seeger C

更新日期：1994-12-01 00:00:00

abstract：:The pregenomic RNA (pgRNA) of hepatitis B virus (HBV) serves as the messenger for both core and P proteins, with the downstream P gene translated by ribosomal leaky scanning. HBV replication begins with packaging of the pgRNA and P protein into core protein particles, followed by conversion of RNA into DNA. Genotype G...

journal_title：Journal of virology

authors： Gutelius D,Li J,Wands J,Tong S

更新日期：2011-12-01 00:00:00

abstract：:Murine cytomegalovirus (MCMV) proteins m142 and m143 are essential for viral replication. They bind double-stranded RNA and prevent protein kinase R-induced protein synthesis shutoff. Whether the two viral proteins have additional functions such as their homologs in human cytomegalovirus do remained unknown. We show t...

journal_title：Journal of virology

authors： Ostermann E,Warnecke G,Waibler Z,Brune W

更新日期：2015-10-28 00:00:00

abstract：:Envelope glycoprotein (Env) reactivity (ER) describes the propensity of human immunodeficiency virus type 1 (HIV-1) Env to change conformation from the metastable unliganded state in response to the binding of ligands (antibodies and soluble CD4 [sCD4]) or incubation in the cold. To investigate Env properties that fav...

journal_title：Journal of virology

authors： McGee K,Haim H,Korioth-Schmitz B,Espy N,Javanbakht H,Letvin N,Sodroski J

更新日期：2014-01-01 00:00:00

abstract：:We have previously described several human immunodeficiency virus type 1 (HIV-1) mutants that are characterized by an excessive-RNA-splicing phenotype and reduced virus particle production. In one of these mutants (NLD2up), the sequence of 5' splice site D2 was changed to a consensus splice donor site. This splice sit...

journal_title：Journal of virology

authors： Mandal D,Feng Z,Stoltzfus CM

更新日期：2008-02-01 00:00:00

abstract：:Herpesviruses encode a set of core proteins essential for lytic replication of their genomes. Three of these proteins form a tripartite helix-primase complex that, in the case of Epstein-Barr virus (EBV), consists of the helicase BBLF4, the primase BSLF1, and the linker protein BBLF2/3. BBLF2/3 and its homologs in the...

journal_title：Journal of virology

authors： Liao G,Huang J,Fixman ED,Hayward SD

更新日期：2005-01-01 00:00:00

abstract：:Monoclonal antibodies (MAbs) directed against the E2 glycoprotein of mouse hepatitis virus (MHV) have been classified according to their ability to bind to either of the two purified 90,000-molecular-weight subunits (90K subunits) of the 180K peplomeric glycoprotein E2. Correlation with previously reported information...

journal_title：Journal of virology

authors： Weismiller DG,Sturman LS,Buchmeier MJ,Fleming JO,Holmes KV

更新日期：1990-06-01 00:00:00

abstract：:The subgroup A to E avian sarcoma and leukosis viruses (ASLVs) are highly related and are thought to have evolved from a common ancestor. These viruses use distinct cell surface proteins as receptors to gain entry into avian cells. Chickens have evolved resistance to infection by the ASLVs. We have identified the muta...

journal_title：Journal of virology

authors： Elleder D,Melder DC,Trejbalova K,Svoboda J,Federspiel MJ

更新日期：2004-12-01 00:00:00

abstract：:F proteins from baculovirus nucleopolyhedrovirus (NPV) group II members are the major budded virus (BV) viral envelope fusion proteins. They undergo furin-like proteolysis processing in order to be functional. F proteins from different baculovirus species have a long cytoplasmic tail domain (CTD), ranging from 48 (Spo...

journal_title：Journal of virology

authors： Long G,Pan X,Westenberg M,Vlak JM

更新日期：2006-11-01 00:00:00

abstract：:In resting CD4(+) T lymphocytes harboring human immunodeficiency virus type 1 (HIV-1), replication-competent virus persists in patients responding to highly active antiretroviral therapy (HAART). This small latent reservoir represents between 10(3) and 10(7) cells per patient. However, the efficiency of HIV-1 DNA-posi...

journal_title：Journal of virology

authors： Fondere JM,Petitjean G,Huguet MF,Salhi SL,Baillat V,Macura-Biegun A,Becquart P,Reynes J,Vendrell JP

更新日期：2004-10-01 00:00:00