Abstract:
:We have identified a novel brain-derived single-stranded-DNA-binding protein that interacts with a region of the human neurotropic JC virus enhancer designated the lytic control element (LCE). This nuclear factor, LCP-1 (for lytic control element-binding protein 1), specifically recognizes the LCE, as determined by gel retardation assays. Alkylation interference showed that specific nucleotides within the LCE were contacted by LCP-1. Subsequent experiments revealed that point mutations within the LCE differentially affected LCP-1 binding. UV cross-linking and competition analysis suggested that the LCP-1 DNA-protein complexes were 50 to 52 and 100 to 120 kDa in size. Promoter mutations that affected LCP-1 binding reduced early mRNA transcription during the early phase of the lytic cycle. However, upon DNA replication in the presence of JC virus T antigen, when early mRNA initiation shifts to new locations indicative of the late phase, the LCP-1 mutations had no effect. We suggest that the JC virus early transcription unit is differentially regulated by LCP-1 prior to but not after DNA replication, suggesting a novel mechanism by which DNA structure regulates eukaryotic gene expression.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tada H,Khalili Kdoi
10.1128/JVI.66.12.6885-6892.1992subject
Has Abstractpub_date
1992-12-01 00:00:00pages
6885-92issue
12eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::The RNA genome of human immunodeficiency virus type 1 (HIV-1) is converted into DNA after infection in order to integrate into the host cell DNA. However, a large number of these reverse-transcribed genomes remain unintegrated in the nucleus of infected cells. Currently, there are no data available about the intranucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7683-7691.2001
更新日期:2001-08-01 00:00:00
abstract::The Epstein-Barr virus (EBV) is an oncogenic human herpesvirus. EBV latent membrane protein 1 (LMP-1) is a viral oncogene that manifests its oncogenic phenotype through activation of cellular signaling pathways involved in cell growth, survival, differentiation, and transformation. Lytic LMP-1 (lyLMP-1) is a related E...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00180-06
更新日期:2006-08-01 00:00:00
abstract::A substantial number of viruses have been demonstrated to subvert autophagy to promote their own replication. Recent publications have reported the proviral effect of autophagy induction on hepatitis B virus (HBV) replication. Hepatitis delta virus (HDV) is a defective virus and an occasional obligate satellite of HBV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01936-19
更新日期:2020-01-31 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02821-06
更新日期:2007-04-01 00:00:00
abstract::Experiments with St. Louis encephalitis (SLE) virus have shown that neutral red enhances the plaque size in duck embryo cell cultures. This may represent a new method for genetic studies of SLE virus population. In a mosquito pool specimen NR(+) and NR(-) particles were demonstrated. By intracerebral passage in mice, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.3.178-181.1968
更新日期:1968-03-01 00:00:00
abstract::A Sepharose 4B chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.3.923-927.1978
更新日期:1978-03-01 00:00:00
abstract::A restriction endonuclease obtained from Haemophilus gallinarum (hgaI) cleaves polyoma DNA at four specific sites. Using the EcoRI, HindIII, and HpaII endonuclease restriction sites as reference, the four HgaI cleavage sites were mapped at 0.02, 0.14, 0.27, and 0.48 fractional lengths, clockwise, from the single EcoR...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.793-798.1976
更新日期:1976-05-01 00:00:00
abstract::We have expressed the human immunodeficiency virus type 1 (HIV-1) protease (PR) in bacteria as a Gag-PR polyprotein (J. Luban and S.P. Goff, J. Virol. 65:3203-3212, 1991). The protein displays enzymatic activity, cleaving the Gag polyprotein precursor Pr55gag to the expected products. The PR enzyme is only active as a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3630-3634.1993
更新日期:1993-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.796-801.1977
更新日期:1977-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6725-6731.1998
更新日期:1998-08-01 00:00:00
abstract::Mucosal surfaces are the primary portals for human immunodeficiency virus (HIV) transmission. Because systemic immunization, in general, does not induce effective mucosal immune responses, a mucosal HIV vaccine is urgently needed. For this study, we developed papillomavirus pseudoviruses that express HIV-1 Gag. The ps...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10249-10257.2004
更新日期:2004-10-01 00:00:00
abstract::HA20, a mutant of Bacillus subtilis phage SPO1, is deficient in both DNA replication and late transcription. HA20 contains mutations in two different genes, which suggested that the two effects might be caused independently. However, single-mutation derivatives, affected only in gene 27, were deficient for both activi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.300-301.1984
更新日期:1984-01-01 00:00:00
abstract::Small interfering RNAs (siRNAs) processed from double-stranded RNA (dsRNA) of virus origins mediate potent antiviral defense through a process referred to as RNA interference (RNAi) or RNA silencing in diverse organisms. In the simple invertebrate Caenorhabditis elegans, the RNAi process is initiated by a single Dicer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01305-13
更新日期:2013-10-01 00:00:00
abstract::The DNA synthesized in the nonpermissive host by the noncomplementing mutants am36 and am42 of bacteriophage phi W-14 contains about half the wild-type level of alpha-putrescinylthymine (putThy) and a correspondingly greater level of thymine. The mechanisms whereby thymine nucleotides are excluded from replicating DNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.3.399-405.1983
更新日期:1983-09-01 00:00:00
abstract::The ORF3 protein of hepatitis E virus (HEV) is a multifunctional protein important for virus replication. The ORF3 proteins from human, swine, and avian strains of HEV contain a conserved PXXP amino acid motif, resembling either Src homology 3 (SH3) cell signaling interaction motifs or "late domains" involved in host ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06711-11
更新日期:2012-05-01 00:00:00
abstract::A short sequence, located between the A14L and A15L open reading frames (ORFs) of vaccinia virus, was predicted to encode a hydrophobic protein of 53 amino acids that is conserved in orthopoxviruses, leporipoxviruses, yatapoxiruses, and molluscipoxviruses. We constructed a recombinant vaccinia virus with a 10-codon ep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4085-4092.2000
更新日期:2000-05-01 00:00:00
abstract::The E5 oncoprotein of bovine papillomavirus type 1 is a 44-amino-acid, hydrophobic polypeptide which localizes predominantly in Golgi membranes and appears to transform cells through the activation of tyrosine kinase growth factor receptors. In fibroblasts, E5 interacts with both the 16-kilodalton vacuolar ATPase subu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2420-2430.1996
更新日期:1996-04-01 00:00:00
abstract::The deoxyribonucleic acid (DNA) of Escherichia coli B is converted by colicin E2 to products soluble in cold trichloroacetic acid; we show that this DNA degradation (hereafter termed solubilization) is subject to inhibition by infection with bacteriophage T4. At least two modes of inhibition may be differentiated on t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.3.303-310.1971
更新日期:1971-09-01 00:00:00
abstract::A vaccinia-directed deoxyribonucleic acid (DNA) polymerase has been partially purified from the cytoplasmic fractions of virus-infected HeLa cells. The utilization of natural and synthetic templates by this enzyme resembles that of the host cell DNA-dependent DNA polymerases. The vaccinia DNA polymerase cannot copy ri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.4.721-729.1972
更新日期:1972-10-01 00:00:00
abstract::Ebola virus is the etiologic agent of a lethal hemorrhagic fever in humans and nonhuman primates with mortality rates of up to 90%. Previous studies with Zaire Ebola virus (ZEBOV), mouse-adapted virus (MA-ZEBOV), and mutant viruses (ZEBOV-NP(ma), ZEBOV-VP24(ma), and ZEBOV-NP/VP24(ma)) allowed us to identify the mutati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00659-11
更新日期:2011-09-01 00:00:00
abstract::The endogenous transcriptase present in purified vesicular stomatitis (VS) virions was solubilized with a Triton X-100 high-salt solution. The polymerase activity was purified on glycerol gradients and by phosphocellulose column chromatography; the viral proteins present in the active enzyme fractions were identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1325-1335.1973
更新日期:1973-12-01 00:00:00
abstract::Infectious cDNA clones of mild (CHV1-Euro7) and severe (CHV1-EP713) hypovirus strains responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus Cryphonectria parasitica were used to construct viable chimeric viruses. Differences in virus-mediated alterations of fungal colony morphology, growt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.16.7562-7567.2000
更新日期:2000-08-01 00:00:00
abstract::The biological activity encoded in the putative protease gene (pro) of human T-cell leukemia virus type I was investigated by using a vaccinia virus expression vector. The 53-kilodalton gag precursor polyprotein was processed into the mature p19, p24, and p15 gag proteins when the gag and protease-coding sequence was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3718-3728.1988
更新日期:1988-10-01 00:00:00
abstract::Adenovirus (Ad) entry into cells is initiated by the binding of the fiber knob to a cell surface receptor. The coxsackie- and adenovirus receptor (CAR) functions as the attachment receptor for many, but not all, Ad serotypes. Ad type 37 (Ad37), a subgroup D virus that causes keratoconjunctivitis in humans, does not in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5375-5380.2001
更新日期:2001-06-01 00:00:00
abstract::Lipid rafts are proposed to function as platforms for both receptor signaling and trafficking. Following interaction with antigenic peptides, the T-cell receptor (TCR) rapidly translocates to lipid rafts, where it transmits signals and subsequently undergoes endocytosis. The Tip protein of herpesvirus saimiri (HVS), w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.16.9041-9051.2003
更新日期:2003-08-01 00:00:00
abstract::Mutations in several subgenomic regions of hepatitis C virus (HCV) have been implicated in influencing the response to interferon (IFN) therapy. Sequences within HCV NS5A (PKR binding domain [PKRBD], IFN sensitivity-determining region [ISDR], and variable region 3 [V3]) were analyzed for the pretreatment serum samples...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02231-07
更新日期:2008-07-01 00:00:00
abstract::The genomic RNA of human T-cell leukemia virus type 1 encodes three polyproteins, Gag, Gag-Pro, and Gag-Pro-Pol, which are translated as a result of no, one, and two frameshifts, respectively. In this report we demonstrate that the 77 residues encoded at the C terminus of the Gag-Pro precursor can be collectively dete...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.13101-13105.2002
更新日期:2002-12-01 00:00:00
abstract::Previous findings of low levels of reverse transcriptase (RT) activity in chick cell-derived measles and mumps vaccines showed this activity to be associated with virus particles containing RNA of both subgroup E endogenous avian leukosis viruses (ALV-E) and endogenous avian viruses (EAV). These particles originate fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3605-3612.2001
更新日期:2001-04-01 00:00:00
abstract::Upon cell entry, the genomes of herpes simplex virus type 1 (HSV-1) and adenovirus (Ad) associate with distinct nuclear structures termed ND10 or promyelocytic leukemia (PML) nuclear bodies (NBs). PML NB morphology is altered or disrupted by specific viral proteins as replication proceeds. We examined whether adeno-as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.1.389-398.2004
更新日期:2004-01-01 00:00:00
abstract::Evidence is presented that, in frog virus 3-infected BHK cells, inhibition of ribonucleic acid (RNA) synthesis is paralleled by a decreased activity of host cell RNA polymerase II, while the template ability of host cell nuclei and chromatin is unaffected. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.698-700.1972
更新日期:1972-04-01 00:00:00