Abstract:
:Functions of viral proteins can be regulated through phosphorylation by serine/threonine kinases in plants, but little is known about the involvement of tyrosine kinases in plant virus infection. In this study, TGBp3, one of the three movement proteins encoded by a triple gene block (TGB) of Potato mop-top virus (PMTV), was detected for the first time in PMTV-infected plants and found to be tyrosine phosphorylated. Phosphorylation sites (Tyr(87-89) and Tyr(120)) were located in two amino acid motifs conserved in the TGB-containing, rod-shaped plant viruses. Substitution of these tyrosine residues in both motifs was needed to abolish tyrosine phosphorylation of TGBp3. Substitution of Tyr(87-89) with alanine residues enhanced the interaction between TGBp3 and TGBp2 and inhibited cell-to-cell movement of PMTV. On the other hand, substitution of Tyr(120) with alanine resulted in no alteration in the interaction of TGBp3 with TGBp2, but the mutant virus was not infectious. The results suggest that tyrosine phosphorylation is a mechanism regulating the functions of plant virus movement proteins.
journal_name
J Viroljournal_title
Journal of virologyauthors
Samuilova O,Santala J,Valkonen JPdoi
10.1128/JVI.03388-12subject
Has Abstractpub_date
2013-04-01 00:00:00pages
4313-21issue
8eissn
0022-538Xissn
1098-5514pii
JVI.03388-12journal_volume
87pub_type
杂志文章abstract::Bicistronic RNAs containing the 373-nucleotide-long 5' nontranslated region (NTR) of the classical swine fever virus (CSFV) genome as intercistronic spacer were used to show the presence of an internal ribosome entry site (IRES) in the 5' end of the CSFV genome. By coexpression of the poliovirus 2A protease it was dem...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.451-457.1997
更新日期:1997-01-01 00:00:00
abstract::Among Moloney murine leukemia viruses (Mo-MuLVs) having stop codons other than UAG at the gag-pol junction, Mo-MuLV with UAA, but not with UGA, had a replication disadvantage. Mo-MuLV with a glutamine codon (CAG) at the junction did not replicate. A revertant of this virus consisted of the original virus and a virus w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6376-6379.1991
更新日期:1991-11-01 00:00:00
abstract::Immunization of mice with DNA encoding the influenza virus hemagglutinin (HA) affords complete protection against lethal influenza virus infection and the means to investigate the mechanisms of B-cell responsiveness to virus challenge. Using a single-cell enzyme-linked immunospot assay, we sought to determine the loca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7712-7717.1995
更新日期:1995-12-01 00:00:00
abstract::The immediate-early gene ie2 of murine cytomegalovirus was characterized. The 1.75-kb ie2 transcript is spliced from three exons, of 78, 124, and 1,283 nucleotides, which are separated by introns of 1,245 and 364 nucleotides. An ATG codon located in the third exon leads into an open reading frame of 391 codons. Immedi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1638-1643.1991
更新日期:1991-03-01 00:00:00
abstract::Accumulated evidence demonstrates that Japanese encephalitis virus (JEV) infection triggers endoplasmic reticulum (ER) stress and neuron apoptosis. ER stress sensor protein kinase R-like endoplasmic reticulum kinase (PERK) has been reported to induce apoptosis under acute or prolonged ER stress. However, the precise r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00887-19
更新日期:2019-08-13 00:00:00
abstract::The ability of the gamma(1)34.5 protein to suppress the PKR response plays a crucial role in herpes simplex virus pathogenesis. In this process, the gamma(1)34.5 protein associates with protein phosphatase 1 to form a large complex that dephosphorylates eIF-2alpha and thereby prevents translation shutoff mediated by P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7653-7666.2004
更新日期:2004-07-01 00:00:00
abstract::Interferon regulatory factor 7 (IRF-7) is implicated in the regulation of Epstein-Barr virus (EBV) latency. EBV transforms primary B cells, and the major EBV oncoprotein, latent membrane protein 1 (LMP-1), is required for the process. LMP-1 both induces the expression of IRF-7 and activates the IRF-7 protein by phosph...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.12987-12995.2004
更新日期:2004-12-01 00:00:00
abstract::A total of 51 restriction sites were recognized within the BK virus genome by the combination of 10 different restriction endonucleases. These sites were mapped and oriented relative to one another as well as to the five fragments generated by the digestion of BK virus DNA with HindIII and EcoRI. The result was a comp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.915-925.1979
更新日期:1979-03-01 00:00:00
abstract::Analysis of the pronase-derived glycopeptides of isolated mumps virus glycoproteins revealed the presence of both complex and high-mannose-type oligosaccharides on the HN and F1 glycoproteins, whereas only high-mannose-type glycopeptides were detected on F2. Endoglycosidase F, a newly described glycosidase that cleave...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.2.354-362.1983
更新日期:1983-08-01 00:00:00
abstract::Among several proposed cellular receptors for bovine viral diarrhea virus (BVDV), the low-density lipoprotein (LDL) receptor is of special interest because it is also considered a receptor for the related hepatitis C virus. It has been reported that an anti-LDL receptor monoclonal antibody blocked the infection of bov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01589-06
更新日期:2006-11-01 00:00:00
abstract::The yield of G418-resistant Huh7 cell clones bearing subgenomic dicistronic GB virus B (GBV-B) is significantly affected by the insertion of a portion of the viral core gene between the GBV-B 5' untranslated region and the exogenous neomycin phosphotransferase selector gene (A. De Tomassi, M. Pizzuti, R. Graziani, A. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7502-7509.2003
更新日期:2003-07-01 00:00:00
abstract::The endogenous transcriptase present in purified vesicular stomatitis (VS) virions was solubilized with a Triton X-100 high-salt solution. The polymerase activity was purified on glycerol gradients and by phosphocellulose column chromatography; the viral proteins present in the active enzyme fractions were identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1325-1335.1973
更新日期:1973-12-01 00:00:00
abstract::Highly conserved amino acids in the second helix structure of the human immunodeficiency virus type 1 (HIV-1) MA protein were identified to be critical for the incorporation of viral Env proteins into HIV-1 virions from transfected COS-7 cells. The effects of these MA mutations on viral replication in the HIV-1 natura...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1443-1452.1997
更新日期:1997-02-01 00:00:00
abstract::ICP0 is a transcriptional activating protein required for the efficient replication and reactivation of latent herpes simplex virus 1 (HSV-1). Multiple regions of ICP0 contribute its activity, the most prominent of which appears to be the RING finger, which confers E3 ubiquitin ligase activity. A region in the C termi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02265-10
更新日期:2011-05-01 00:00:00
abstract::The fundamental question of whether a primed immune system is capable of preventing latent gammaherpesvirus infection remains unanswered. Recent studies showing that vaccination can reduce acute replication and short-term latency but cannot alter long-term latency further call into question the possibility of achievin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2522-2529.2003
更新日期:2003-02-01 00:00:00
abstract::Small aggregates of viral antigen were encountered in the nuclear matrix. The capsids did not tag with antibodies specific for the virus or for the host cell. This observation remains unexplained. Nuclear and cytoplasmic membranes, as well as the envelope of the virus, reacted with both types of antibodies and appear,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.10.1172-1184.1968
更新日期:1968-10-01 00:00:00
abstract::Acute measles in children can be prevented by immunization with the live attenuated measles vaccine virus. Although immunization is able to induce CD4 and CD8 T cells as well as neutralizing antibodies, only the latter have been correlated with protective immunity. CD8 T cells, however, have been documented to be impo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00160-07
更新日期:2007-08-01 00:00:00
abstract::CD8+ T cells were previously shown to be important in preventing lymphoproliferation and immunodeficiency following infection of murine AIDS (MAIDS)-resistant mice with the LP-BM5 mixture of murine leukemia viruses. To further evaluate the mechanisms contributing to MAIDS resistance, we studied mice lacking CD8+ T cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1808-1813.1997
更新日期:1997-03-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) infects alveolar macrophages (AMϕ), causing dysregulated alpha interferon (IFN-α) and tumor necrosis factor alpha (TNF-α) production through a mechanism(s) yet to be resolved. Here, we show that AMϕ infected with PRRSV secreted a reduced quantity of IFN-α fol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01251-17
更新日期:2018-01-02 00:00:00
abstract::The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls viral gene transcription in a cell-type-specific manner. The HPV-18 URR is active in HeLa cells but inactive in HepG2 cells. The activating activity of YY1 in HeLa cells is dependent on its functional interactions with the switch regio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7695-7705.1996
更新日期:1996-11-01 00:00:00
abstract::T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.1155-1159.1976
更新日期:1976-06-01 00:00:00
abstract::Antigens detected by the complement-fixation (CF) test were prepared from BHK-21 cells infected with Pichinde virus. The preparations contained two antigens demonstrable by immunodiffusion. The antigen present in abundance was heat stable, Pronase resistant, and had a molecular weight of 20,000 to 30,000 as estimated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.1.175-186.1977
更新日期:1977-04-01 00:00:00
abstract::Residual hepatitis B virus (HBV) DNA can be detected in serum and liver after apparent recovery from transient infection. However, it is not known if this residual HBV DNA represents ongoing viral replication and antigen expression. In the current study, ducks inoculated with duck hepatitis B virus (DHBV) were monitor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.19.12242-12252.2005
更新日期:2005-10-01 00:00:00
abstract::Knowledge of the mechanisms of virus dissemination in acute measles is cursory, but cells of the monocyte/macrophage (MM) lineage appear to be early targets. We characterized the dissemination of the Edmonston B vaccine strain of measles virus (MV-Ed) in peripheral blood mononuclear cells (PBMC) of two mouse strains e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3343-3351.2001
更新日期:2001-04-01 00:00:00
abstract::The tsB5 mutant of herpes simplex virus type 1 (HSV-1) strain HFEM was shown previously to be temperature sensitive for accumulation of the mature form of glycoprotein gB, for production or activity of a factor required in virus-induced cell fusion, and for production of virions with normal levels of infectivity. In a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.114-128.1980
更新日期:1980-07-01 00:00:00
abstract:UNLABELLED:Human T-cell lymphotropic virus type 1 (HTLV-1) and HTLV-2 encode auxiliary proteins that play important roles in viral replication, viral latency, and immune escape. The presence of auxiliary protein-encoding open reading frames (ORFs) in HTLV-3, the latest HTLV to be discovered, is unknown. Simian T-cell l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02150-14
更新日期:2015-01-15 00:00:00
abstract::Like almost all of the positive-strand RNA viruses, hepatitis C virus (HCV) induces host intracellular membrane modification to form the membrane-bound viral replication complex (RC), within which viral replicases amplify the viral RNA genome. Despite accumulated information about how HCV co-opts host factors for vira...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01140-16
更新日期:2016-10-14 00:00:00
abstract::Multispecific CD8(+) T-cell responses are thought to be important for the control of acute hepatitis C virus (HCV) infection, but to date little information is actually available on the breadth of responses at early time points. Additionally, the influence of early therapy on these responses and their relationships to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12979-12988.2005
更新日期:2005-10-01 00:00:00
abstract:UNLABELLED:The structure of adenovirus outer capsid was revealed recently at 3- to 4-Å resolution (V. Reddy, S. Natchiar, P. Stewart, and G. Nemerow, Science 329:1071-1075, 2010, http://dx.doi.org/10.1126/science.1187292); however, precise details on the function and biochemical and structural features for the inner co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00935-14
更新日期:2014-08-01 00:00:00
abstract::The US3 protein kinase of herpes simplex virus 1 blocks apoptosis induced by replication-incompetent virus mutants, proapoptotic members of the Bcl-2 family of proteins, and by a variety of other agents that act at the premitochondrial level in the proapoptotic cascade. To define the role of US3 in blocking apoptosis ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00820-07
更新日期:2007-10-01 00:00:00