Abstract:
:Recognition of viruses by germ line-encoded pattern recognition receptors of the innate immune system is essential for rapid production of type I interferon (IFN) and early antiviral defense. We investigated the mechanisms of viral recognition governing production of type I IFN during herpes simplex virus (HSV) infection. We show that early production of IFN in vivo is mediated through Toll-like receptor 9 (TLR9) and plasmacytoid dendritic cells, whereas the subsequent alpha/beta IFN (IFN-alpha/beta) response is derived from several cell types and induced independently of TLR9. In conventional DCs, the IFN response occurred independently of viral replication but was dependent on viral entry. Moreover, using a HSV-1 UL15 mutant, which fails to package viral DNA into the virion, we found that entry-dependent IFN induction also required the presence of viral genomic DNA. In macrophages and fibroblasts, where the virus was able to replicate, HSV-induced IFN-alpha/beta production was dependent on both viral entry and replication, and ablated in cells unable to signal through the mitochondrial antiviral signaling protein pathway. Thus, during an HSV infection in vivo, multiple mechanisms of pathogen recognition are active, which operate in cell-type- and time-dependent manners to trigger expression of type I IFN and coordinate the antiviral response.
journal_name
J Viroljournal_title
Journal of virologyauthors
Rasmussen SB,Sørensen LN,Malmgaard L,Ank N,Baines JD,Chen ZJ,Paludan SRdoi
10.1128/JVI.01167-07subject
Has Abstractpub_date
2007-12-01 00:00:00pages
13315-24issue
24eissn
0022-538Xissn
1098-5514pii
JVI.01167-07journal_volume
81pub_type
杂志文章abstract::The adenovirus type 5 mutant dl1520 was engineered previously to be completely defective for E1B-55K functions. Recently, this mutant (also known as ONYX-015) has been suggested to replicate preferentially in p53(-) and some p53(+) tumor cell lines but to be attenuated in primary cultured cells (C. Heise, A. Sampson-J...
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pub_type: 杂志文章
doi:10.1128/JVI.73.7.5333-5344.1999
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doi:10.1128/JVI.02532-08
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更新日期:2016-06-10 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4407-4414.2005
更新日期:2005-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00407-07
更新日期:2007-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4297-4307.1998
更新日期:1998-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.601-610.1985
更新日期:1985-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.1.104-112.1982
更新日期:1982-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.8.6.922-924.1971
更新日期:1971-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.457-467.1978
更新日期:1978-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4539-4545.2003
更新日期:2003-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.951-957.1979
更新日期:1979-12-01 00:00:00
abstract::The high rate of HIV-1 evolution contributes to immune escape, enables the virus to escape drug therapy, and may underlie the difficulty of producing an effective vaccine. Identifying constraints on HIV evolution is therefore of prime importance. To investigate this problem, we examined the relationships between seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00702-10
更新日期:2010-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.10.4376-4385.1989
更新日期:1989-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00241-14
更新日期:2014-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1782-1789.1994
更新日期:1994-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3129-3140.2001
更新日期:2001-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,评审
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更新日期:2015-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.13943-13952.2005
更新日期:2005-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.516-529.1993
更新日期:1993-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.1924-1928.1989
更新日期:1989-05-01 00:00:00
abstract::Nonenveloped viruses often invade membranes by exposing hydrophobic or amphipathic peptides generated by a proteolytic maturation step that leaves a lytic peptide noncovalently associated with the viral capsid. Since multiple copies of the same protein form many nonenveloped virus capsids, it is unclear if lytic pepti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01089-12
更新日期:2012-09-01 00:00:00
abstract::To determine the extent and structure of genetic variation in dengue viruses (DENV) on a restricted spatial and temporal scale, we sequenced the E (envelope) genes of DENV-1, -2, and -3 isolates collected in 2001 from children enrolled in a prospective school-based study in Kamphaeng Phet, Thailand, and diagnosed with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02728-07
更新日期:2008-06-01 00:00:00
abstract::Measles virus (MV) can infect the central nervous system and, in rare cases, causes subacute sclerosing panencephalitis, characterized by a progressive degeneration of neurons. The route of MV transmission in neurons was investigated in cultured rat hippocampal slices by using MV expressing green fluorescent protein. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5720-5728.2002
更新日期:2002-06-01 00:00:00
abstract::Active proteinase 3C of hepatitis A virus (HAV) was expressed in bacteria either as a mature enzyme or as a protein fused to the entire polymerase 3D or to a part of it, and their identities were shown by immunoblot analysis. Intermolecular cleavage activity was demonstrated by incubating in vitro-translated and radio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6794-6796.1992
更新日期:1992-11-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) mutants with codon insertions and deletions in IE-0, the gene encoding ICP0, were constructed. The HSV-1 deletion mutant dl1403 (N. D. Stow and E. C. Stow, J. Gen. Virol. 67:2571-2585, 1986) and an IE-0:lacZ transplacement vector isolated in this study were used to facilitate the co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.2916-2927.1992
更新日期:1992-05-01 00:00:00
abstract::The development of cancer vaccines requires approaches to induce expansion and functional differentiation of tumor antigen-specific cytotoxic T lymphocyte (CTL) effectors which posses cytolytic capability and produce cytokines. Efficient induction of such cells is hindered by the poor immunogenicity of tumor antigens ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7411-7424.2003
更新日期:2003-07-01 00:00:00
abstract::Integrase of human immunodeficiency virus type 1 (HIVIN) consists of 288 amino acids, and its minimum DNA-binding domain (MDBD) (amino acids [aa] 220 to 270) is required for the integration reaction. We produced and characterized four murine monoclonal antibodies (MAbs) to the MDBD of HIVIN (strain LAI). Immunoblot an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4475-4480.1999
更新日期:1999-05-01 00:00:00
abstract::SE21Q1b, a Rous sarcoma virus mutant which packages cellular rather than viral RNA, is competent for infection of quail cells and can transmit defective transforming retrovirus genes. Stably transformed recipient clones have been obtained by using this mutant. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.380-382.1981
更新日期:1981-04-01 00:00:00