Abstract:
:Integrase of human immunodeficiency virus type 1 (HIVIN) consists of 288 amino acids, and its minimum DNA-binding domain (MDBD) (amino acids [aa] 220 to 270) is required for the integration reaction. We produced and characterized four murine monoclonal antibodies (MAbs) to the MDBD of HIVIN (strain LAI). Immunoblot and enzyme-linked immunosorbent assays with truncated HIVINs showed that those MAbs recognized sequential epitopes within the MDBD (aa 228 to 236, 237 to 252, 253 to 261, and 262 to 270). Their binding to HIVIN inhibited terminal cleavage and strand transfer activities but not disintegration activity in vitro. This collection of MAbs is useful for studying the structure and function of the MDBD by complementing mutational analyses and other biochemical studies.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ishikawa T,Okui N,Kobayashi N,Sakuma R,Kitamura T,Kitamura Ydoi
10.1128/JVI.73.5.4475-4480.1999subject
Has Abstractpub_date
1999-05-01 00:00:00pages
4475-80issue
5eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::Cloned avian leukosis viral DNAs were mutagenized in the long terminal repeat, in the leader sequence for env mRNA, and at the poly-env junction. The effect of these mutations in the viral DNA upon its ability to direct virus production or env mRNA synthesis was analyzed by microinjecting the mutant DNAs into chicken ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.2.503-510.1982
更新日期:1982-08-01 00:00:00
abstract::Rodent fibroblasts infected with the ts371 Kirsten murine sarcoma virus (KiMuSV) are temperature sensitive for the maintenance of transformation because of the production of an abnormal p21 protein. We cloned the ts371 KiMuSV provirus from the genome of a conditionally transformed nonproducer cell line, ts371 KiMuSV N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.782-786.1986
更新日期:1986-11-01 00:00:00
abstract::The Arabidopsis flowering locus T (FT) gene encodes the mobile florigen essential for floral induction. While movement of the FT protein has been shown to occur within plants, systemic spread of FT mRNA remains to be unequivocally demonstrated. Utilizing novel RNA mobility assay vectors based on two distinct movement-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02346-08
更新日期:2009-04-01 00:00:00
abstract::The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters em...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01259-13
更新日期:2013-09-01 00:00:00
abstract::Previous analyses using in situ hybridization alone or together with PCR have yielded conflicting results regarding the cell type in which latent varicella-zoster virus (VZV) resides. We separated human trigeminal ganglia (TG) into neuronal and nonneuronal fractions, followed by primary and nested PCR to quantitate VZ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8571-8577.1999
更新日期:1999-10-01 00:00:00
abstract::Human trophoblast cells were permissively infected by human cytomegalovirus. The kinetics of viral immediate-early, early, and late gene expression was clearly delayed compared to that in fibroblasts. Productive infection was unequivocally proven by the detection of virion particles, infectious virus in trophoblast cu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7598-7602.1998
更新日期:1998-09-01 00:00:00
abstract::The Sindbis virus minimal subgenomic mRNA promoter (spanning positions -19 to +5 relative to the subgenomic mRNA start site) is approximately three- to sixfold less active than the fully active -98 to +14 promoter region. We identified two elements flanking the -19 to +5 region which increase its transcription to leve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3509-3519.2001
更新日期:2001-04-01 00:00:00
abstract::Identifying protease cleavage sites contributes to our understanding of their specificity and biochemical properties and can help in designing specific inhibitors. One route to this end is the generation and screening of random libraries of cleavage sites. Both synthetic and phage-displayed libraries have been extensi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10563-10570.2000
更新日期:2000-11-01 00:00:00
abstract::Vaccinia virus replication is inhibited by etoposide and mitoxantrone even though poxviruses do not encode the type II topoisomerases that are the specific targets of these drugs. Furthermore, one can isolate drug-resistant virus carrying mutations in the viral DNA ligase and yet the ligase is not known to exhibit sen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02723-07
更新日期:2008-06-01 00:00:00
abstract::Influenza A virus infection can arrest autophagy, as evidenced by autophagosome accumulation in infected cells. Here, we report that this autophagosome accumulation can be inhibited by amantadine, an antiviral proton channel inhibitor, in amantadine-sensitive virus infected cells or cells expressing influenza A virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00576-15
更新日期:2015-10-14 00:00:00
abstract::The human polyomavirus JC virus (JCV) establishes persistent infections in most individuals and is the etiologic agent of progressive multifocal leukoencephalopathy. In this report, we describe the establishment of a soluble cell-free system that is capable of replicating exogenous plasmid DNA containing the JCV origi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7421-7428.1997
更新日期:1997-10-01 00:00:00
abstract::Nucleocytoplasmic shuttling and interaction with the cellular mRNA export factor UAP56 are prerequisites for the mRNA export activity of human cytomegalovirus (HCMV) pUL69. Although the murine cytomegalovirus homolog pM69 shuttles, it fails to export mRNAs due to its inability to recruit UAP56. However, chimeric prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00730-12
更新日期:2012-07-01 00:00:00
abstract::In coliphage 186, 22 essential genes were defined by complementation studies with amber mutants. Eighteen genes were associated with phage morphogenesis: 11 with phage tail formation, and 7 with phage head formation. The remaining four genes are discussed in the accompanying paper (S. M. Hocking and J. B. Egan, J. Vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.1056-1067.1982
更新日期:1982-12-01 00:00:00
abstract::Human herpesvirus 8 (HHV-8) is likely to be involved in the pathogenesis of Kaposi's sarcoma (KS) and body cavity-based lymphomas (BCBLs). The HHV-8 genome is primarily in a latent state in BCBL-derived cell lines like BCBL-1, but lytic replication can be induced by phorbol esters (R. Renne, W. Zhang, B. Herndier, M. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6725-6731.1998
更新日期:1998-08-01 00:00:00
abstract::Live attenuated human immunodeficiency virus type 1 (HIV-1) vaccines are considered unsafe because faster-replicating pathogenic virus variants may evolve after vaccination. We previously presented a conditional-live HIV-1 variant of which replication can be switched off as an alternative vaccination strategy. To impr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3855-3858.2005
更新日期:2005-03-01 00:00:00
abstract::Flock house virus is a small insect virus with a bipartite RNA genome consisting of RNA1 and RNA2. RNA3 is a subgenomic element encoded by RNA1, the genomic segment required for viral RNA synthesis (T. M. Gallagher, P. D. Friesen, and R. R. Rueckert, J. Virol. 46:481-489, 1983). Synthesis of RNA3 is strongly inhibited...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2716-2722.1993
更新日期:1993-05-01 00:00:00
abstract::TMC125 is a potent new investigational nonnucleoside reverse transcriptase inhibitor (NNRTI) that is active against human immunodeficiency virus type 1 (HIV-1) with resistance to currently licensed NNRTIs. Sequential passage experiments with both wild-type virus and NNRTI-resistant virus were performed to identify mut...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12773-12782.2005
更新日期:2005-10-01 00:00:00
abstract::Chimeric bovine respiratory syncytial viruses (BRSV) expressing glycoproteins of bovine parainfluenza virus type 3 (BPIV-3) instead of BRSV glycoproteins were generated from cDNA. In the BRSV antigenome cDNA, the open reading frames of the major BRSV glycoproteins, attachment protein G and fusion protein F, were repla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9367-9377.2001
更新日期:2001-10-01 00:00:00
abstract::We have identified nucleotide sequences that regulate transcription in both a cell-type-specific and general manner in the long terminal repeat of the MCF13 murine leukemia virus. Besides the enhancer element, we have observed that the region between the enhancer and promoter (DEN) has a profound effect on transcripti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7080-7088.1992
更新日期:1992-12-01 00:00:00
abstract::Infection of the chestnut blight fungus Cryphonectria parasitica with Cryphonectria hypovirus 1 (CHV1) causes disruption of virulence, pigmentation, and sporulation. Transcriptional downregulation of key developmentally regulated fungal genes occurs during infection, but vegetative growth is unaffected. Previous studi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05756-11
更新日期:2012-06-01 00:00:00
abstract::To clarify the role of core protein phosphorylation in pregenomic-RNA encapsidation of human and duck hepatitis B viruses (HBV and DHBV, respectively), we have examined the phosphorylation states of different forms of intracellular HBV core protein and the phenotypic effects of mutations in the phosphorylation sites o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4721-4728.2000
更新日期:2000-05-01 00:00:00
abstract::Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.11890-11903.2004
更新日期:2004-11-01 00:00:00
abstract::Type 1 human immunodeficiency viruses encoding mutated nef reading frames are 10- to 30-fold less infectious than are isogenic viruses in which the nef gene is intact. This defect in infectivity causes nef-negative viruses to grow at an attenuated rate in vitro. To investigate the mechanism of Nef-mediated enhancement...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4283-4290.1996
更新日期:1996-07-01 00:00:00
abstract::The mechanism of lysis by in vivo-induced cytotoxic T lymphocytes (CTL) was examined with virus-specific CTL from mice infected with lymphocytic choriomeningitis virus (LCMV). LCMV-induced T cells were shown to have greater than 10 times the serine esterase activity of T cells from normal mice, and high levels of seri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3726-3733.1990
更新日期:1990-08-01 00:00:00
abstract::The putative envelope 2 (E2) gene of hepatitis C virus (HCV) contains a highly variable region referred to as hypervariable region 1 (HVR1). We hypothesized that this genetic variability is driven by immune selection pressure, rather than representing the accumulation of random mutations in a region with relatively li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.3058-3066.2000
更新日期:2000-04-01 00:00:00
abstract::Transient lymphopenia is a hallmark of measles virus (MV)-induced immunosuppression. To address to what extent replenishment of the peripheral lymphocyte compartment from bone marrow (BM) progenitor/stem cells might be affected, we analyzed the interaction of wild-type MV with hematopoietic stem and progenitor cells (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00532-11
更新日期:2011-08-01 00:00:00
abstract::Transcriptionally active replication complexes bound to smooth membrane vesicles were isolated from poliovirus-infected cells. In electron microscopic, negatively stained preparations, the replication complex appeared as an irregularly shaped, oblong structure attached to several virus-induced vesicles of a rosettelik...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1156-1163.1990
更新日期:1990-03-01 00:00:00
abstract:UNLABELLED:In Kaposi's sarcoma-associated herpesvirus (KSHV), poly(ADP-ribose) polymerase 1 (PARP-1) acts as an inhibitor of lytic replication. Here, we demonstrate that KSHV downregulated PARP-1 upon reactivation. The viral processivity factor of KSHV (PF-8) interacted with PARP-1 and was sufficient to degrade PARP-1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00559-15
更新日期:2015-09-01 00:00:00
abstract::We previously observed that when human immunodeficiency virus (HIV)-infected T lymphocytes are added to epithelial cells, they adhere, polarize, and secrete virions unidirectionally onto the epithelium. Epithelial cells subsequently take up virus and become productively infected. We report here that colchicine treatme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.2898-2905.1994
更新日期:1994-05-01 00:00:00
abstract::A gene encoding a polypeptide with homology to mammalian vascular endothelial growth factors (VEGFs) has been discovered in the genome of orf virus (OV), a parapoxvirus that affects sheep and goats and, occasionally, humans. The gene is transcribed abundantly early in infection and is found immediately outside the inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.84-92.1994
更新日期:1994-01-01 00:00:00