Abstract:
:The mechanism of lysis by in vivo-induced cytotoxic T lymphocytes (CTL) was examined with virus-specific CTL from mice infected with lymphocytic choriomeningitis virus (LCMV). LCMV-induced T cells were shown to have greater than 10 times the serine esterase activity of T cells from normal mice, and high levels of serine esterase were located in the LCMV-induced CD8+ cell population. Serine esterase was also induced in purified T-cell preparations isolated from mice infected with other viruses (mouse hepatitis, Pichinde, and vaccinia). In contrast, the interferon inducer poly(I.C) only marginally enhanced serine esterase in T cells. Serine esterase activity was released from the LCMV-induced T cells upon incubation with syngeneic but not allogeneic LCMV-infected target cells. Both cytotoxicity and the release of serine esterase were calcium dependent. Serine esterase released from disrupted LCMV-induced T cells was in the form of the fast-sedimenting particles, suggesting its inclusion in granules. Competitive substrates for serine esterase blocked killing by LCMV-specific CTL, but serine esterase-containing granules isolated from LCMV-induced CTL, in contrast to granules isolated from a rat natural killer cell tumor line, did not display detectable hemolytic activity. Fragmentation of target cell DNA was observed during the lytic process mediated by LCMV-specific CTL, and the release of the DNA label [125I]iododeoxyuridine from target cells and the accompanying fragmentation of DNA also were calcium dependent. These data support the hypothesis that the mechanism of killing by in vivo-induced T cells involves a calcium-dependent secretion of serine esterase-containing granules and a target cell death by a process involving nuclear degradation and DNA fragmentation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Welsh RM,Nishioka WK,Antia R,Dundon PLdoi
10.1128/JVI.64.8.3726-3733.1990subject
Has Abstractpub_date
1990-08-01 00:00:00pages
3726-33issue
8eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::Mumps virus, like other paramyxoviruses in the Rubulavirus genus, encodes a V protein that can assemble a ubiquitin ligase complex from cellular components, leading to the destruction of cellular signal transducer and activator of transcription (STAT) proteins. While many V proteins target the interferon-activated STA...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.6052-6056.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01638-19
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1828-1834.1999
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.99-107.1977
更新日期:1977-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1468-1474.1994
更新日期:1994-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.02097-15
更新日期:2015-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.69.11.6859-6864.1995
更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.3.569-574.1975
更新日期:1975-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.545-551.1988
更新日期:1988-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.7106-7112.2003
更新日期:2003-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.2.220-227.1972
更新日期:1972-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6757-6764.1997
更新日期:1997-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00340-07
更新日期:2007-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4436-4441.1993
更新日期:1993-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.971-978.2001
更新日期:2001-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2614-2620.1987
更新日期:1987-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.6.1254-1262.1974
更新日期:1974-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2020-03-17 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3185-3195.1998
更新日期:1998-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.705-709.1979
更新日期:1979-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.1.125-133.1987
更新日期:1987-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00820-07
更新日期:2007-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.753-758.1993
更新日期:1993-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.6620-6630.2005
更新日期:2005-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12773-12782.2005
更新日期:2005-10-01 00:00:00