Base composition and hybridization studies of the three double-stranded RNA segments of bacteriophage phi 6.


:The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of the large dsRNA segment. The two smaller RNA segments reanneal rapidly even at low temperatures; in contrast, the large dsRNA reannealed only at higher temperatures. Evidence is also presented which suggests that the dsRNAs may contain a short single-stranded RNA tail.


J Virol


Journal of virology


Van Etten JL,Vidaver AK,Koski RK,Burnett JP




Has Abstract


1974-06-01 00:00:00












  • Induction of an organ-specific autoimmune disease, lymphocytic hypophysitis, in hamsters by recombinant rubella virus glycoprotein and prevention of disease by neonatal thymectomy.

    abstract::Glycosylated, membrane-associated E1 (58-kDa) and E2 (47- to 49-kDa) rubella virus proteins and unglycosylated nucleoprotein C (33 kDa), from separately expressed vaccinia virus recombinants, were injected into golden Syrian hamsters. Rubella virus E1 and E2 glycoproteins consistently induced an organ-specific autoimm...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yoon JW,Choi DS,Liang HC,Baek HS,Ko IY,Jun HS,Gillam S

    更新日期:1992-02-01 00:00:00

  • Role of immunoproteasome catalytic subunits in the immune response to hepatitis B virus.

    abstract::Inhibition of hepatitis B virus (HBV) replication and viral clearance from an infected host requires both the innate and adaptive immune responses. Expression of interferon (IFN)-inducible proteasome catalytic and regulatory subunits correlates with the IFN-alpha/beta- and IFN-gamma-mediated noncytopathic inhibition o...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Robek MD,Garcia ML,Boyd BS,Chisari FV

    更新日期:2007-01-01 00:00:00

  • Development of an Alternative Modified Live Influenza B Virus Vaccine.

    abstract::Influenza B virus (IBV) is considered a major human pathogen, responsible for seasonal epidemics of acute respiratory illness. Two antigenically distinct IBV hemagglutinin (HA) lineages cocirculate worldwide with little cross-reactivity. Live attenuated influenza virus (LAIV) vaccines have been shown to provide better...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Santos JJS,Finch C,Sutton T,Obadan A,Aguirre I,Wan Z,Lopez D,Geiger G,Gonzalez-Reiche AS,Ferreri L,Perez DR

    更新日期:2017-05-26 00:00:00

  • Optimal replication activity of vesicular stomatitis virus RNA polymerase requires phosphorylation of a residue(s) at carboxy-terminal domain II of its accessory subunit, phosphoprotein P.

    abstract::The phosphoprotein, P, of vesicular stomatitis virus (VSV) is a key subunit of the viral RNA-dependent RNA polymerase complex. The protein is phosphorylated at multiple sites in two different domains. We recently showed that specific serine and threonine residues within the amino-terminal acidic domain I of P protein ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hwang LN,Englund N,Das T,Banerjee AK,Pattnaik AK

    更新日期:1999-07-01 00:00:00

  • Mechanism of reduced T-cell effector functions and class-switched antibody responses to herpes simplex virus type 2 in the absence of B7 costimulation.

    abstract::T-cell costimulation molecules B7-1 and B7-2 play an important role in activation of T cells to cytolytic effector function and production of cytokines. Interaction with B7 also causes T cells to upregulate surface molecules, such as CD40L, that effectively stimulate antibody responses in conjunction with cytokines. W...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Thebeau LG,Morrison LA

    更新日期:2003-02-01 00:00:00

  • Two point mutations produce infectious retrovirus bearing a green fluorescent protein-SU fusion protein.

    abstract::Two second-site mutations in Moloney murine leukemia virus envelope surface protein (SU) were previously shown to rescue infection of two different SU mutants, a fusion-defective point mutant and a fusion-defective modified SU that exhibits weak subunit association. We report here that they also rescue infection of a ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kizhatil K,Gromley A,Albritton LM

    更新日期:2001-12-01 00:00:00

  • Dengue Virus Targets Nrf2 for NS2B3-Mediated Degradation Leading to Enhanced Oxidative Stress and Viral Replication.

    abstract::Dengue virus (DENV) is a mosquito-borne virus that infects upward of 300 million people annually and has the potential to cause fatal hemorrhagic fever and shock. While the parameters contributing to dengue immunopathogenesis remain unclear, the collapse of redox homeostasis and the damage induced by oxidative stress ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ferrari M,Zevini A,Palermo E,Muscolini M,Alexandridi M,Etna MP,Coccia EM,Fernandez-Sesma A,Coyne C,Zhang DD,Marques ETA,Olagnier D,Hiscott J

    更新日期:2020-11-23 00:00:00

  • Ternary complex factors and cofactors are essential for human T-cell leukemia virus type 1 tax transactivation of the serum response element.

    abstract::The human T-cell leukemia virus type 1 Tax protein activates the expression of cellular immediate early genes controlled by the serum response element (SRE), which contains both the serum response factor (SRF) binding element (CArG box) and the ternary complex factor (TCF) binding element (Ets box). We show that TCF b...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Shuh M,Derse D

    更新日期:2000-12-01 00:00:00

  • Rapamycin-resistant mTORC1 kinase activity is required for herpesvirus replication.

    abstract::Human cytomegalovirus (HCMV) infection has been shown to activate the mTORC1 signaling pathway. However, the phosphorylation of mTORC1 targets is differentially sensitive to the mTORC1 inhibitor rapamycin, and the drug inhibits HCMV replication to a modest extent. Using Torin1, a newly developed inhibitor that targets...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Moorman NJ,Shenk T

    更新日期:2010-05-01 00:00:00

  • Protective effect of biological response modifiers on murine cytomegalovirus infection.

    abstract::Pretreatment with two biological response modifiers (BRM), OK-432 and PS-K, protected mice from lethal infection by murine cytomegalovirus (MCMV). This was evidenced by an increase in 50% lethal doses and a decrease in titers of infectious viruses replicated in the liver and spleen. Spleen cells from the BRM-treated m...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ebihara K,Minamishima Y

    更新日期:1984-07-01 00:00:00

  • Two specific topoisomerase II inhibitors prevent replication of human cytomegalovirus DNA: an implied role in replication of the viral genome.

    abstract::In this study, we show that human cytomegalovirus DNA synthesis is inhibited in infected confluent human embryonic lung cells treated with the DNA-intercalative topoisomerase II inhibitor 4-9'-(acridinylamino)methanesulfon-m-anisidide (m-AMSA). Similar inhibitory effects were observed with VM-26, a nonintercalative to...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Benson JD,Huang ES

    更新日期:1988-12-01 00:00:00

  • A switch region determines the cell type-specific positive or negative action of YY1 on the activity of the human papillomavirus type 18 promoter.

    abstract::YY1 is a zinc finger transcription factor which acts as either a repressor or an activator dependent on the promoter context. YY1 is a potent activator of the genuine human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) in HeLa cells, which are known for high-level expression of the HPV-18 early gene...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bauknecht T,Jundt F,Herr I,Oehler T,Delius H,Shi Y,Angel P,Zur Hausen H

    更新日期:1995-01-01 00:00:00

  • Activation of ATPase activity of simian virus 40 large T antigen by the covalent affinity analog of ATP, fluorosulfonylbenzoyl 5'-adenosine.

    abstract::Fluorosulfonylbenzoyl 5'-adenosine (FSBA) bound to one site in simian virus 40 large T antigen (T) and covalently modified greater than 95% of the molecules in a complete reaction. This analog for ATP specifically cross-links to the Mg-phosphate pocket in ATP-binding sites. Cyanogen bromide cleavage and tryptic digest...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bradley MK

    更新日期:1990-10-01 00:00:00

  • Antibody-mediated enhancement of human immunodeficiency virus type 1 infectivity is determined by the structure of gp120 and depends on modulation of the gp120-CCR5 interaction.

    abstract::In this study, we characterized the viral determinants of coreceptor usage in relation to susceptibility to antibody-mediated neutralization or enhancement of infectivity by using chimeras of three highly related human immunodeficiency virus type 1 (HIV-1) isolates of different phenotypes. We found that the V3 region ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Guillon C,Schutten M,Boers PH,Gruters RA,Osterhaus AD

    更新日期:2002-03-01 00:00:00

  • Identification, characterization, and sequence analysis of a cDNA encoding a phosphoprotein of human herpesvirus 6.

    abstract::Human herpesvirus 6 (HHV-6)-specific monoclonal antibody (Mab) 9A5D12 reacted with the nucleus of HHV-6 strain GS-infected cells and immunoprecipitated a phosphorylated polypeptide with an approximate size of 41 kDa, designated HHV-6 P41. A 110-kDa polypeptide was also immunoprecipitated by the MAb. These polypeptides...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Chang CK,Balachandran N

    更新日期:1991-06-01 00:00:00

  • An overlapping bacterial artificial chromosome system that generates vectorless progeny for channel catfish herpesvirus.

    abstract::Herpesviruses are important pathogens of humans and other animals. Herpesvirus infectious clones that can reconstitute phenotypically wild-type (wt) virus are extremely valuable tools for elucidating the roles of specific genes in virus pathophysiology as well as for making vaccines. Ictalurid herpesvirus 1 (channel c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kunec D,Hanson LA,van Haren S,Nieuwenhuizen IF,Burgess SC

    更新日期:2008-04-01 00:00:00

  • bcl-2 alters influenza virus yield, spread, and hemagglutinin glycosylation.

    abstract::We previously demonstrated that expression of bcl-2 in Madin-Darby canine kidney (MDCK) cells blocks influenza virus-induced apoptosis and DNA fragmentation. We show here that expression of bcl-2 also reduces the level of infectious virus production and the spread of virus in MDCK cell cultures infected at a low multi...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Olsen CW,Kehren JC,Dybdahl-Sissoko NR,Hinshaw VS

    更新日期:1996-01-01 00:00:00

  • Activity of rat Mx proteins against a rhabdovirus.

    abstract::Upon stimulation with alpha/beta interferon, rat cells synthesize three Mx proteins. Sequence analysis of corresponding cDNAs reveals that these three proteins are derived from three distinct genes. One of the rat cDNAs is termed Mx1 because it is most closely related to the mouse Mx1 cDNA and because it codes for a n...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Meier E,Kunz G,Haller O,Arnheiter H

    更新日期:1990-12-01 00:00:00

  • Switch to unusual amino acids at codon 215 of the human immunodeficiency virus type 1 reverse transcriptase gene in seroconvertors infected with zidovudine-resistant variants.

    abstract::Sequences of the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) domain were determined by direct sequencing of HIV-1 RNA in successive plasma samples from eight seroconverting patients infected with virus bearing the T215Y/F amino acid substitution associated with zidovudine (ZDV) resistance. A...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yerly S,Rakik A,De Loes SK,Hirschel B,Descamps D,Brun-Vézinet F,Perrin L

    更新日期:1998-05-01 00:00:00

  • The avian influenza virus nucleoprotein gene and a specific constellation of avian and human virus polymerase genes each specify attenuation of avian-human influenza A/Pintail/79 reassortant viruses for monkeys.

    abstract::Reassortant viruses which possessed the hemagglutinin and neuraminidase genes of wild-type human influenza A viruses and the remaining six RNA segments (internal genes) of the avian A/Pintail/Alberta/119/79 (H4N6) virus were previously found to be attenuated in humans. To study the genetic basis of this attenuation, w...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Snyder MH,Buckler-White AJ,London WT,Tierney EL,Murphy BR

    更新日期:1987-09-01 00:00:00

  • The human papillomavirus type 16 E7 oncoprotein activates the Fanconi anemia (FA) pathway and causes accelerated chromosomal instability in FA cells.

    abstract::Fanconi anemia (FA) patients have an increased risk for squamous cell carcinomas (SCCs) at sites of predilection for infection with high-risk human papillomavirus (HPV) types, including the oral cavity and the anogenital tract. We show here that activation of the FA pathway is a frequent event in cervical SCCs. We fou...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Spardy N,Duensing A,Charles D,Haines N,Nakahara T,Lambert PF,Duensing S

    更新日期:2007-12-01 00:00:00

  • Epstein-Barr Virus BKRF4 Gene Product Is Required for Efficient Progeny Production.

    abstract::Epstein-Barr virus (EBV), a member of human gammaherpesvirus, infects mainly B cells. EBV has two alternative life cycles, latent and lytic, and is reactivated occasionally from the latent stage to the lytic cycle. To combat EBV-associated disorders, understanding the molecular mechanisms of the EBV lytic replication ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Masud HMAA,Watanabe T,Yoshida M,Sato Y,Goshima F,Kimura H,Murata T

    更新日期:2017-11-14 00:00:00

  • Herpes simplex virus tegument protein VP22 contains an internal VP16 interaction domain and a C-terminal domain that are both required for VP22 assembly into the virus particle.

    abstract::Many steps along the herpesvirus assembly and maturation pathway remain unclear. In particular, the acquisition of the virus tegument is a poorly understood process, and the molecular interactions involved in tegument assembly have not yet been defined. Previously we have shown that the two major herpes simplex virus ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hafezi W,Bernard E,Cook R,Elliott G

    更新日期:2005-10-01 00:00:00

  • Characterization of vesicular stomatitis virus mutants by partial proteolysis.

    abstract::Structural proteins of temperature-sensitive (ts) mutants of vesicular stomatitis virus, Indiana serotype, were compared with those of wild-type and revertant virions by electrophoresis on polyacrylamide gels of partial digests with Staphylococcus aureus V8 protease. Mutants of complementation groups III (tsG31 and ts...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Metzel PS,Reichmann ME

    更新日期:1981-01-01 00:00:00

  • Location of the ss--mutation of bacteriophage T7 in genes 10, the structural gene for the major capsid protein.

    abstract::T7+ phage are unable to plate on a strain of Shigella sonnei D2 371-48. Spontaneous phage mutants arise (ss--mutants) that are able to plate on this strain of Shigella. We have shown by complementation studies and genetic crosses that the ss--mutation maps in gene 10, the structural gene for the major protein of the c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Vetter D,Roberts L,Jackowski JB,Sadowski PD

    更新日期:1977-06-01 00:00:00

  • Human immunodeficiency virus type 1 Tat increases the expression of cleavage and polyadenylation specificity factor 73-kilodalton subunit modulating cellular and viral expression.

    abstract::The human immunodeficiency virus type 1 (HIV-1) Tat protein, which is essential for HIV gene expression and viral replication, is known to mediate pleiotropic effects on various cell functions. For instance, Tat protein is able to regulate the rate of transcription of host cellular genes and to interact with the signa...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Calzado MA,Sancho R,Muñoz E

    更新日期:2004-07-01 00:00:00

  • Respiratory syncytial virus (RSV) G glycoprotein is not necessary for vaccine-enhanced disease induced by immunization with formalin-inactivated RSV.

    abstract::Following respiratory syncytial virus (RSV) challenge, mice immunized with RSV G or with formalin-inactivated RSV (FI-RSV) exhibit severe disease associated with type 2 cytokine production and pulmonary eosinophilia. This has led to the proposal that the presence of RSV G is the factor in FI-RSV that induces disease-e...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Johnson TR,Teng MN,Collins PL,Graham BS

    更新日期:2004-06-01 00:00:00

  • The PU.1/Spi-1 proto-oncogene is a transcriptional regulator of a lentivirus promoter.

    abstract::The enhancer unit present in the retrovirus equine infectious anemia virus (EIAV) was previously shown to contain binding sites for proteins belonging to MDBP, PEA2, AP-1, and ets families. The EIAV ets motif matches the consensus sequence for both PEA3- and PU.1-binding sites. Here, we show by gel shift analysis that...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Carvalho M,Derse D

    更新日期:1993-07-01 00:00:00

  • Inferring Transmission Bottleneck Size from Viral Sequence Data Using a Novel Haplotype Reconstruction Method.

    abstract::The transmission bottleneck is defined as the number of viral particles that transmit from one host to establish an infection in another. Genome sequence data have been used to evaluate the size of the transmission bottleneck between humans infected with the influenza virus; however, the methods used to make these est...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ghafari M,Lumby CK,Weissman DB,Illingworth CJR

    更新日期:2020-06-16 00:00:00

  • Induction of protective immunity against Japanese encephalitis in mice by immunization with a plasmid encoding Japanese encephalitis virus premembrane and envelope genes.

    abstract::A DNA vaccine plasmid containing the Japanese encephalitis (JE) virus premembrane (prM) and envelope (E) genes (designated pcDNA3JEME) was evaluated for immunogenicity and protective efficacy in mice. Two immunizations of 4-week-old female ICR mice with pcDNA3JEME by intramuscular or intradermal injections at a dose o...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Konishi E,Yamaoka M,Khin-Sane-Win,Kurane I,Mason PW

    更新日期:1998-06-01 00:00:00