Abstract:
:The four major Epstein-Barr virion envelope components were separated by column chromatography and reconstituted into artificial liposomes. These liposomes were tested for their ability to bind selectively to Epstein-Barr virus receptor-positive cells. Only when the two high-molecular-weight glycoproteins, VE1 and VE2, were present together was a stable binding complex formed. The addition of the other virion envelope components did not increase the levels of binding. This binding was inhibited by unlabeled viable virions and by neutralizing antisera, which recognized the two components. Adsorption of viable virus was also eliminated by the antisera. The enzyme susceptibility pattern of the cell-liposome interaction is similar to that of the virus-cell interaction, thus confirming the specificity of the binding site. A model for Epstein-Barr virus binding in which VE1 and VE2 coordinately recognize the same binding site is presented.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wells A,Koide N,Klein Gdoi
10.1128/JVI.41.1.286-297.1982subject
Has Abstractpub_date
1982-01-01 00:00:00pages
286-97issue
1eissn
0022-538Xissn
1098-5514journal_volume
41pub_type
杂志文章abstract:UNLABELLED:We searched The Cancer Genome Atlas (TCGA) database for viruses by comparing non-human reads present in transcriptome sequencing (RNA-Seq) and whole-exome sequencing (WXS) data to viral sequence databases. Human papillomavirus 18 (HPV18) is an etiologic agent of cervical cancer, and as expected, we found rob...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03365-14
更新日期:2015-04-01 00:00:00
abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) K1 gene encodes a polypeptide bearing an immunoreceptor tyrosine-based activation motif (ITAM) that is constitutively active for ITAM-based signal transduction. Although ectopic overexpression of K1 in cultured fibroblasts can lead to growth transformation, in vivo th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5891-5898.2001
更新日期:2001-07-01 00:00:00
abstract::We have previously reported on the ubiquitylation and degradation of hepatitis C virus core protein. Here we demonstrate that proteasomal degradation of the core protein is mediated by two distinct mechanisms. One leads to polyubiquitylation, in which lysine residues in the N-terminal region are preferential ubiquityl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01690-08
更新日期:2009-03-01 00:00:00
abstract::The ability of certain vesicular stomatitis virus (VSV; Indiana serotype) temperature-sensitive (ts) mutants to synthesize intracellular viral complementary RNA (vcRNA) at permissive or nonpermissive temperatures for productive infections has been investigated. Mutants belonging to complementation groups II, III, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.157-169.1976
更新日期:1976-10-01 00:00:00
abstract::Endogenous retroviruses, records of past retroviral infections, are ubiquitous in vertebrate genomes. On occasion, vertebrate hosts have co-opted retroviral genes for their own biological functions. Here, we perform a phylogenomic survey of retroviral gag gene homologs within vertebrate genomes and identify two ancien...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00542-19
更新日期:2019-06-28 00:00:00
abstract:UNLABELLED:In a previous screen of putative interferon-stimulated genes, SUN2 was shown to inhibit HIV-1 infection in an uncharacterized manner. SUN2 is an inner nuclear membrane protein belonging to the linker of nucleoskeleton and cytoskeleton complex. We have analyzed here the role of SUN2 in HIV infection. We repor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03202-15
更新日期:2016-03-28 00:00:00
abstract::CD8+ T cells are the key cellular effectors mediating the clearance of hepatitis B virus (HBV) infections. However, early immunological events surrounding the priming of HBV-specific CD8+ T cell responses remain poorly understood. This study examined the importance of priming location and the relative contribution of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00920-18
更新日期:2018-10-12 00:00:00
abstract:UNLABELLED:The distribution of vesicular stomatitis virus (VSV) nucleocapsids in the cytoplasm of infected cells was analyzed by scanning confocal fluorescence microscopy using a newly developed quantitative approach called the border-to-border distribution method. Nucleocapsids were located near the cell nucleus at ea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00488-16
更新日期:2016-06-10 00:00:00
abstract::Reverse transcription in avian sarcoma virus (ASV) initiates from the 3' end of a tRNA(Trp) primer, which anneals near the 5' end of the RNA genome. The region around the primer-binding site (PBS) forms an elaborate stem structure composed of the U5-inverted repeat (U5-IR) stem, the U5-leader stem, and the association...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7571-7577.2002
更新日期:2002-08-01 00:00:00
abstract::The generation of cytopathic effects by murine leukemia viruses (MLVs) in different cell types correlates with the ability of the virus to induce thymic lymphoma. We showed that the induction of apoptosis in mink epithelial cells by mink cell focus-forming (MCF) MLV infection results in the accumulation of high levels...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02291-07
更新日期:2008-03-01 00:00:00
abstract::The copy choice model for the generation of defective interfering (DI) particles of vesicular stomatitis virus suggests that during replication the polymerase prematurely terminates, moves with the nascent daughter strand to another site on the same or a different template molecule, and resumes elongation of the nasce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.2.515-521.1984
更新日期:1984-08-01 00:00:00
abstract::The wild-type strain of vesicular stomatitis virus (VSV) contains in its complete virion (VSV-1, B particles) a minus strand RNA. The principle defective particle of the wild-type strain (VSV-111, T particles) contains a shorter minus strand, homologous to part of the VSV-1 genome. Neither virion contains any detectab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.6.915-925.1973
更新日期:1973-06-01 00:00:00
abstract::The sole immediate-early (IE) gene of equine herpesvirus 1 encodes a 1,487-amino-acid (aa) regulatory phosphoprotein that independently activates expression of early viral genes. Coimmunoprecipitation assays demonstrated that the IE protein physically interacts with the general transcription factor TFIIB. Using a vari...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10219-10230.2001
更新日期:2001-11-01 00:00:00
abstract::Despite intensive study, the mechanism by which many retroviruses complete reverse transcription has remained unclear. Most retroviruses and all lentiviruses fail to synthesize a full-length second strand of the viral cDNA (plus strand) efficiently in infected cells. For human immunodeficiency virus type 1, we find in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3938-3944.1995
更新日期:1995-06-01 00:00:00
abstract::Receptors (FcgammaRs) for the constant region of immunoglobulin G (IgG) are an important link between humoral immunity and cellular immunity. To help define the role of FcgammaRs in determining the fate of human immunodeficiency virus type 1 (HIV-1) immune complexes, cDNAs for the four major human Fcgamma receptors (F...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00656-09
更新日期:2009-08-01 00:00:00
abstract::The v-src coding region was deleted from cloned Rous sarcoma virus DNA, and the deleted clones were tested for infectivity by transfection. All of the coding region, as well as most of the sequences lying between v-src and the unique 3' region (U3), could be deleted without affecting viability. However, at least 9 and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.2.482-488.1982
更新日期:1982-08-01 00:00:00
abstract::The Epstein-Barr virus (EBV) lytic activator genes bzlf1 and brlf1 are conventionally referred to as immediate-early (IE) genes. However, previous studies showed that the earliest expression of these genes was blocked by cycloheximide when the EBV lytic cycle was induced by histone deacetylase (HDAC) inhibitors and pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01415-10
更新日期:2010-12-01 00:00:00
abstract::The host cell receptor for Moloney murine leukemia virus was solubilized from murine L-cell membranes and characterized. In initial studies designed to identify a receptor-rich cell line, different mouse cells were screened for binding to Moloney gp70, the viral envelope glycoprotein which determines host cell-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.900-908.1986
更新日期:1986-06-01 00:00:00
abstract::ICP27 is a multifunctional protein that is required for herpes simplex virus 1 mRNA export. ICP27 interacts with the mRNA export receptor TAP/NXF1 and binds RNA through an RGG box motif. Unlike other RGG box proteins, ICP27 does not bind G-quartet structures but instead binds GC-rich sequences that are flexible in str...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00509-10
更新日期:2010-07-01 00:00:00
abstract::Viruses actively interact with host metabolism because viral replication relies on host cells to provide nutrients and energy. Vaccinia virus (VACV; the prototype poxvirus) prefers glutamine to glucose for efficient replication to the extent that VACV replication is hindered in glutamine-free medium. Remarkably, our d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01834-18
更新日期:2019-06-14 00:00:00
abstract::pp60v-src is a nonreceptor protein tyrosine kinase that can transform both chicken and rodent fibroblasts. The src homology 2 (SH2) domain of this protein serves a critical role in the regulation of protein tyrosine kinase activity. The host range proteins pp60v-src-L, which contains a deletion of a highly conserved r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7267-7274.1994
更新日期:1994-11-01 00:00:00
abstract::Rhesus lymphocryptovirus (rLCV) and Epstein-Barr virus (EBV) are closely related gammaherpesviruses that infect and cause disease in rhesus monkeys and humans, respectively. Thus, rLCV is an important model system for EBV pathogenesis. Both rLCV and EBV express microRNAs (miRNAs), several conserved in sequence and gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00110-10
更新日期:2010-05-01 00:00:00
abstract::Poliovirus proteins 3A and 3AB are small, membrane-binding proteins that play multiple roles in viral RNA replication complex formation and function. In the infected cell, these proteins associate with other viral and cellular proteins as part of a supramolecular complex whose structure and composition are unknown. We...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02398-10
更新日期:2011-05-01 00:00:00
abstract::A fresh inoculum of human adenovirus type 12 (Ad12) was obtained from the American Type Culture Collection and passaged once on human embryonic kidney cells, and Ad12 DNA was prepared from the first-passage yield to avoid higher passages which might have generated host-virus DNA recombinants. The 18 PstI fragments of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.379-389.1994
更新日期:1994-01-01 00:00:00
abstract::Animal influenza viruses pose a clear threat to public health. Transmissibility among humans is a prerequisite for a novel influenza virus to cause a human pandemic. A novel reassortant swine influenza virus acquired sustained human-to-human transmissibility and caused the 2009 influenza pandemic. However, the molecul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00958-12
更新日期:2012-09-01 00:00:00
abstract::The three primary capsid proteins (A, B, and C) of adeno-associated viruses have been shown previously to contain overlapping amino acid sequences (R. McPherson and J. Rose, J. Virol. 46:523-529, 1983). In the present study we demonstrate definitively that these proteins are encoded in the right half of the adeno-asso...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.591-597.1984
更新日期:1984-11-01 00:00:00
abstract::Expression of the two bovine papillomavirus type 1 (BPV-1) late genes, L1 and L2, coding for the two capsid proteins, is limited to terminally differentiated keratinocytes in bovine fibropapillomas. This pattern of expression is determined both by the activity of the late promoter and by the inhibition of late region ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.5806-5812.1991
更新日期:1991-11-01 00:00:00
abstract::The DNA synthesized in the nonpermissive host by the noncomplementing mutants am36 and am42 of bacteriophage phi W-14 contains about half the wild-type level of alpha-putrescinylthymine (putThy) and a correspondingly greater level of thymine. The mechanisms whereby thymine nucleotides are excluded from replicating DNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.3.399-405.1983
更新日期:1983-09-01 00:00:00
abstract::Suppression of interferon signaling is of paramount importance to a virus. Interferon signaling significantly reduces or halts the ability of a virus to replicate; therefore, viruses have evolved sophisticated mechanisms that suppress activation of the interferon pathway or responsiveness of the infected cell to inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-16
更新日期:2017-03-29 00:00:00
abstract::Senecavirus A (SVA) is a picornavirus that causes acute vesicular disease (VD), that is clinically indistinguishable from foot-and-mouth disease (FMD), in pigs. Notably, SVA RNA has been detected in lymphoid tissues of infected animals several weeks following resolution of the clinical disease, suggesting that the vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00819-19
更新日期:2019-10-15 00:00:00