Abstract:
:Despite intensive study, the mechanism by which many retroviruses complete reverse transcription has remained unclear. Most retroviruses and all lentiviruses fail to synthesize a full-length second strand of the viral cDNA (plus strand) efficiently in infected cells. For human immunodeficiency virus type 1, we find in synchronous infection experiments that full-length plus strands are rare (< 1% of products) at times when integration is likely taking place. Subviral nucleoprotein complexes containing such discontinuous cDNA can be extracted from infected cells and used to generate integration products in vitro. Analysis of such integration products using two-dimensional gel electrophoresis revealed that the discontinuous viral DNA was efficiently integrated into an added target DNA. These data support a model in which the discontinuities in the plus strand need not be sealed until after integration, potentially by the enzymes that are already thought to repair DNA gaps at the junctions between host and viral DNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Miller MD,Wang B,Bushman FDdoi
10.1128/JVI.69.6.3938-3944.1995subject
Has Abstractpub_date
1995-06-01 00:00:00pages
3938-44issue
6eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Cucumber mosaic virus (CMV) is a tripartite RNA virus that can support the replication of satellite RNAs, small molecular parasites of the virus. Satellite RNAs can have a dramatic effect on the helper virus and the host plant in a manner specific to the helper, satellite, and host. Previously, we showed that the Sny-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.608-612.1997
更新日期:1997-01-01 00:00:00
abstract::Recognition of viruses by germ line-encoded pattern recognition receptors of the innate immune system is essential for rapid production of type I interferon (IFN) and early antiviral defense. We investigated the mechanisms of viral recognition governing production of type I IFN during herpes simplex virus (HSV) infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01167-07
更新日期:2007-12-01 00:00:00
abstract::Antigens derived from host cells are detectable in the envelope of human immunodeficiency virus type 1 (HIV-1) and result in a distinctive viral phenotype reflecting that of the host cell. An immunomagnetic capture assay targeting discriminatory host proteins was developed to differentiate between HIV-1 derived from m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Purified Semliki Forest virus (SFV) contains three structural proteins while its core (nucleocapsid) contains two of these proteins. To identify all of the proteins synthesized under virus direction, cells were infected with SFV in the presence of actinomycin D and guanidine. Cell protein synthesis was markedly and ir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.10.1076-1080.1968
更新日期:1968-10-01 00:00:00
abstract::Polymorphic basic residues near the C terminus of the prion protein (PrP) in humans and sheep appear to protect against prion disease. In heterozygotes, inhibition of prion formation appears to be dominant negative and has been simulated in cultured cells persistently infected with scrapie prions. The results of nucle...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4351-4360.2000
更新日期:2000-05-01 00:00:00
abstract::Functions of viral proteins can be regulated through phosphorylation by serine/threonine kinases in plants, but little is known about the involvement of tyrosine kinases in plant virus infection. In this study, TGBp3, one of the three movement proteins encoded by a triple gene block (TGB) of Potato mop-top virus (PMTV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03388-12
更新日期:2013-04-01 00:00:00
abstract::The involvement of the mouse mammary tumor virus (MTV) in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains was studied by comparing the amounts of MTV RNA and MTV DNA sequences in mammary tumors and other tissues of mice with an without hormonal treatments. The following results were o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.551-559.1978
更新日期:1978-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) impacts multiple lineages of hematopoietic cells, including lymphocytes and macrophages, either by direct infection or indirectly by perturbations of cell networks, leading to generalized immune deficiency. We designed a study to discover, in primary human macrophages, senti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.11477-11486.2004
更新日期:2004-11-01 00:00:00
abstract::Influenza viruses unable to express NS1 protein (delNS1) replicate poorly and induce large amounts of interferon (IFN). They are therefore considered candidate viruses for live-attenuated influenza vaccines. Their attenuated replication is generally assumed to result from the inability to counter the antiviral host re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01725-12
更新日期:2012-11-01 00:00:00
abstract::The genetic evolution of human immunodeficiency virus type 1 (HIV-1) in the brain is distinct from that in lymphoid tissues, indicating tissue-specific compartmentalization of the virus. Few primary HIV-1 envelope glycoproteins (Envs) from uncultured brain tissues have been biologically well characterized. In this stu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12336-12345.2003
更新日期:2003-11-01 00:00:00
abstract::The 2009 pandemic influenza virus (pdm/09) has been frequently introduced to pigs and has reassorted with other swine viruses. Recently, H3N2 reassortants with pdm/09-like internal genes were isolated in Guangxi and Hong Kong, China. Genetic and epidemiological analyses suggest that these viruses have circulated in sw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06824-11
更新日期:2012-02-01 00:00:00
abstract::Proviral clones of human immunodeficiency virus type 1 which contained single amino acid changes in the envelope V3 region were constructed. PCR amplification of Sup-T1 T cells transfected with one such mutant, G312T, revealed low levels of virus that resulted in the generation of a revertant virus, in which an alanin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.8380-8385.1994
更新日期:1994-12-01 00:00:00
abstract::Two major DNA variants have been isolated from serially propagated stocks of the WR strain of vaccinia virus. Restriction enzyme mapping of the two variants with HindIII, AvaI, XhoI, SstII, and SmaI revealed a 6.3-megadalton deletion in the smaller DNA variant. The deletion was mapped at ca. 6.8 megadaltons in from th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.1000-1010.1981
更新日期:1981-03-01 00:00:00
abstract::Natural killer (NK) cells derived from the human female reproductive tract (FRT) are phenotypically and functionally distinct from those obtained from peripheral blood. Because the FRT is a primary site of human immunodeficiency virus type 1 (HIV-1) infection in women, we determined whether soluble factors secreted by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00562-09
更新日期:2009-11-01 00:00:00
abstract::We previously described a monoclonal antibody (Y8-10C2) that binds influenza virus hemagglutinin (HA) monomers but not native trimers. In this study, we demonstrated that Y8-10C2 binds to the globular domain of HA and found evidence that its epitope is located at the interface of adjacent subunits. We further showed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.933-942.1993
更新日期:1993-02-01 00:00:00
abstract::The DNA synthesized in the nonpermissive host by the noncomplementing mutants am36 and am42 of bacteriophage phi W-14 contains about half the wild-type level of alpha-putrescinylthymine (putThy) and a correspondingly greater level of thymine. The mechanisms whereby thymine nucleotides are excluded from replicating DNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.3.399-405.1983
更新日期:1983-09-01 00:00:00
abstract::Muscle is an attractive target for gene delivery because of its mass and because vectors can be delivered in a noninvasive fashion. Adeno-associated virus (AAV) has been shown to be effective for muscle-targeted gene transfer. Recent progress in characterization of AAV serotype 1 (AAV1) and AAV6 demonstrated that thes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2768-2774.2003
更新日期:2003-02-01 00:00:00
abstract::The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2494-2501.2004
更新日期:2004-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) SM protein is an essential nuclear shuttling protein expressed by EBV early during the lytic phase of replication. SM acts to increase EBV lytic gene expression by binding EBV mRNAs and enhancing accumulation of the majority of EBV lytic cycle mRNAs. SM increases target mRNA stability and nucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01483-09
更新日期:2009-11-01 00:00:00
abstract::Transgenic (Tg) mice expressing full-length bovine prion protein (BoPrP) serially propagate bovine spongiform encephalopathy (BSE) prions without posing a transmission barrier. These mice also posed no transmission barrier for Suffolk sheep scrapie prions, suggesting that cattle may be highly susceptible to some sheep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5259-5271.2005
更新日期:2005-05-01 00:00:00
abstract::The genome of influenza A virus consists of eight single-strand negative-sense RNA segments, each comprised of a coding region and a noncoding region. The noncoding region of the NS segment is thought to provide the signal for packaging; however, we recently showed that the coding regions located at both ends of the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3766-3774.2005
更新日期:2005-03-01 00:00:00
abstract::There is controversy as to whether the cell entry mechanism of Sindbis virus (SIN) involves direct fusion of the viral envelope with the plasma membrane at neutral pH or uptake by receptor-mediated endocytosis and subsequent low-pH-induced fusion from within acidic endosomes. Here, we studied the membrane fusion activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8476-8484.1999
更新日期:1999-10-01 00:00:00
abstract:UNLABELLED:The HIV-1 virion infectivity factor (Vif) targets the cellular cytidine deaminases APOBEC3G (A3G) and APOBEC3F (A3F) for degradation via the host ubiquitin-proteasome pathway. Vif recruits a cellular E3 ubiquitin ligase to polyubiquitinate A3G/F. The activity of Vif critically depends on the cellular core bi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03824-13
更新日期:2014-03-01 00:00:00
abstract::It has been shown that not all but most of the avian influenza viruses replicate in the upper respiratory tract of pigs (H. Kida et al., J. Gen. Virol. 75:2183-2188, 1994). It was shown that A/chicken/Yamaguchi/7/2004 (H5N1) [Ck/Yamaguchi/04 (H5N1)] did not replicate in pigs (N. Isoda et al., Arch. Virol. 151:1267-127...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01879-08
更新日期:2009-02-01 00:00:00
abstract::The vaccinia virus (VV) A10L gene codes for a major core protein, P4a. This polypeptide is synthesized at late times during viral infection and is proteolytically cleaved during virion assembly. To investigate the role of P4a in the virus life cycle and morphogenesis, we have generated an inducer-dependent conditional...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5778-5795.2001
更新日期:2001-07-01 00:00:00
abstract::The mechanisms involved in the emergence of virulent mammalian viruses were investigated in the adult immunodeficient SCID mouse infected by the attenuated prototype strain of the parvovirus Minute Virus of Mice (MVMp). Cloned MVMp intravenously inoculated in mice consistently evolved during weeks of subclinical infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11280-11290.2005
更新日期:2005-09-01 00:00:00
abstract::Mutants (N(-)nin) of bacteriophage lambda in which the N gene product is not required for growth on wild-type Escherichia coli do not plate on recA bacterial mutants. Secondary mutants, selected for growth on recA, lie within the immunity region to the right of gene cI and appear identical to the cro mutants of Eisen ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.6.938-945.1972
更新日期:1972-06-01 00:00:00
abstract::Hepatitis C virus (HCV), a member of the family Flaviviridae, is a leading cause of chronic liver disease and cancer. Recent advances in HCV therapeutics have resulted in improved cure rates, but an HCV vaccine is not available and is urgently needed to control the global pandemic. Vaccine development has been hampere...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00874-14
更新日期:2014-09-01 00:00:00
abstract::In this study, we show that replication-competent subgenomic hepatitis C virus (HCV) RNA can be transferred to permissive Huh7 cells, leading to the establishment of viral RNA replication. Further, we show that these events are mediated by exosomes rather than infectious virus particles. If similar events occur in viv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02721-14
更新日期:2015-03-01 00:00:00
abstract::Viruses cause about 15% of the cancers that are still the leading causes of human mortality. The discovery of viral oncogenes has enhanced our understanding of viral oncogenesis. However, the underlying molecular mechanisms of virus-induced cancers are complex and require further investigation. The present study has a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01392-10
更新日期:2011-01-01 00:00:00