Abstract:
:ICP27 is a multifunctional protein that is required for herpes simplex virus 1 mRNA export. ICP27 interacts with the mRNA export receptor TAP/NXF1 and binds RNA through an RGG box motif. Unlike other RGG box proteins, ICP27 does not bind G-quartet structures but instead binds GC-rich sequences that are flexible in structure. To determine the contribution of arginines within the RGG box, we performed in vitro binding assays with N-terminal proteins encoding amino acids 1 to 160 of wild-type ICP27 or arginine-to-lysine substitution mutants. The R138,148,150K triple mutant bound weakly to sequences that were bound by the wild-type protein and single and double mutants. Furthermore, during infection with the R138,148,150K mutant, poly(A)(+) RNA and newly transcribed RNA accumulated in the nucleus, indicating that viral RNA export was impaired. To determine if structural changes had occurred, nuclear magnetic resonance (NMR) analysis was performed on N-terminal proteins consisting of amino acids 1 to 160 from wild-type ICP27 and the R138,148,150K mutant. This region of ICP27 was found to be highly flexible, and there were no apparent differences in the spectra seen with wild-type ICP27 and the R138,148,150K mutant. Furthermore, NMR analysis with the wild-type protein bound to GC-rich sequences did not show any discernible folding. We conclude that arginines at positions 138, 148, and 150 within the RGG box of ICP27 are required for binding to GC-rich sequences and that the N-terminal portion of ICP27 is highly flexible in structure, which may account for its preference for binding flexible sequences.
journal_name
J Viroljournal_title
Journal of virologyauthors
Corbin-Lickfett KA,Souki SK,Cocco MJ,Sandri-Goldin RMdoi
10.1128/JVI.00509-10subject
Has Abstractpub_date
2010-07-01 00:00:00pages
6367-76issue
13eissn
0022-538Xissn
1098-5514pii
JVI.00509-10journal_volume
84pub_type
杂志文章abstract::Live attenuated RNA viruses make highly efficient vaccines. Among them, measles virus (MV) vaccine has been given to a very large number of children and has been shown to be highly efficacious and safe. Therefore, this vaccine might be a very promising vector to immunize children against both measles and other infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11546-11554.2003
更新日期:2003-11-01 00:00:00
abstract::Ascoviruses (family Ascoviridae) are double-stranded DNA viruses with circular genomes that attack lepidopterans, where they produce large, enveloped virions, 150 by 400 nm, and cause a chronic, fatal disease with a cytopathology resembling that of apoptosis. After infection, host cell DNA is degraded, the nucleus fra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01639-06
更新日期:2006-12-01 00:00:00
abstract:UNLABELLED:Increasing data suggest that NK cells can mediate antiviral activity in HIV-1-infected humans, and as such, novel approaches harnessing the anti-HIV-1 function of both T cells and NK cells represent attractive options to improve future HIV-1 immunotherapies. Chronic progressive HIV-1 infection has been assoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00924-14
更新日期:2014-08-01 00:00:00
abstract::The large T antigen encoded by simian virus 40 (SV40) plays essential roles in the infection of permissive cells, leading to production of progeny virions, and in the infection of nonpermissive cells, leading to malignant transformation. Primary mouse embryo fibroblasts (MEFs) are nonpermissive for SV40, and infection...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6872-6880.1991
更新日期:1991-12-01 00:00:00
abstract::The family Anelloviridae includes human and animal torque teno viruses (TTVs) with extensive genetic diversity. The antigenic diversity among anelloviruses has never been assessed. Using torque teno sus virus (TTSuV) as a model, we describe here the first investigation of the antigenic relationships among different an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00176-12
更新日期:2012-10-01 00:00:00
abstract::The major phosphoprotein common to woolly monkey sarcoma virus, gibbon ape lymphosarcoma virus, and type C viruses of the lower mammalian species (mouse, rat, cat), with the exception of the endogenous cat virus (RD-114), is the polypeptide of about 12,000 molecular weight. The protein-phosphate bond in this polypepti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.123-131.1975
更新日期:1975-07-01 00:00:00
abstract::The development of neoplasia frequently involves inactivation of the p53 and retinoblastoma (Rb) tumor suppressor pathways and disruption of cell cycle checkpoints that monitor the integrity of replication and cell division. The human papillomavirus type 16 (HPV-16) oncoproteins, E6 and E7, have been shown to bind p53...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.975-985.1998
更新日期:1998-02-01 00:00:00
abstract::Viruses cause about 15% of the cancers that are still the leading causes of human mortality. The discovery of viral oncogenes has enhanced our understanding of viral oncogenesis. However, the underlying molecular mechanisms of virus-induced cancers are complex and require further investigation. The present study has a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01392-10
更新日期:2011-01-01 00:00:00
abstract:UNLABELLED:The live attenuated measles virus vaccine is highly immunostimulatory. Identification and characterization of its components that activate the innate immune response might provide new strategies and agents for the rational design and development of chemically defined adjuvants. In this study, we report on th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02161-15
更新日期:2015-11-25 00:00:00
abstract::To study the role of the biochemical and physiological activities of simian virus 40 (SV40) large T antigen in the lytic and transformation processes, we have analyzed DNA replication-defective, transformation-competent T-antigen mutants. Here we describe two such mutants, C8/SV40 and T22/SV40, and also summarize the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.1.120-127.1985
更新日期:1985-01-01 00:00:00
abstract::Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
abstract::Profiles of bacteriophage T4 early proteins resolved by a two-dimensional nonequilibrium pH gradient electrophoresis system (P. Z. O'Farrell, H. M. Goodman, and P. H. O'Farrell, Cell 12:1133--1142, 1977) are presented. Over 65 phage-induced proteins were resolved. Amber or deletion mutants were used to identify 17 pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.3.767-772.1982
更新日期:1982-06-01 00:00:00
abstract::In resting CD4(+) T lymphocytes harboring human immunodeficiency virus type 1 (HIV-1), replication-competent virus persists in patients responding to highly active antiretroviral therapy (HAART). This small latent reservoir represents between 10(3) and 10(7) cells per patient. However, the efficiency of HIV-1 DNA-posi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10536-10542.2004
更新日期:2004-10-01 00:00:00
abstract::Chinese hamster X mouse somatic cell hybrids were analyzed by Southern blot hybridization with a probe specific for the cellular c-fms proto-oncogene. Results demonstrate that Fms, the genetic locus containing this sequence, maps to mouse chromosome 18. Mouse Fms is thus not linked to the same set of genes involved in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.1055-1056.1988
更新日期:1988-03-01 00:00:00
abstract::The Ebola virus envelope glycoprotein (GP) derived from the pathogenic Zaire subtype mediates cell rounding and detachment from the extracellular matrix in 293T cells. In this study we provide evidence that GPs from the other pathogenic subtypes, Sudan and Côte d'Ivoire, as well as from Reston, a strain thought to be ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.5.2518-2528.2002
更新日期:2002-03-01 00:00:00
abstract::The inhibition of protein synthesis by cycloheximide markedly stimulated adenovirus 2 early transcription (assayed in vivo and in vitro in nuclei) when the drug was added 3 h after infection of HeLa cells. The stimulation was not uniform but ranged from three- to eightfold for region 1A to 10- to 30-fold for region 2 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.683-692.1983
更新日期:1983-02-01 00:00:00
abstract::Previous analyses using in situ hybridization alone or together with PCR have yielded conflicting results regarding the cell type in which latent varicella-zoster virus (VZV) resides. We separated human trigeminal ganglia (TG) into neuronal and nonneuronal fractions, followed by primary and nested PCR to quantitate VZ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8571-8577.1999
更新日期:1999-10-01 00:00:00
abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 57 (ORF57)-encoded protein (Mta) is a multifunctional regulator of viral gene expression. ORF57 is essential for viral replication, so elucidation of its molecular mechanisms is important for understanding KSHV infection. ORF57 has been implicated i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03459-12
更新日期:2013-04-01 00:00:00
abstract::Infectious cDNA clones of mild (CHV1-Euro7) and severe (CHV1-EP713) hypovirus strains responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus Cryphonectria parasitica were used to construct viable chimeric viruses. Differences in virus-mediated alterations of fungal colony morphology, growt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.16.7562-7567.2000
更新日期:2000-08-01 00:00:00
abstract::Three amber mutations were introduced proximal to the syn3 locus of the herpes simplex virus type 1 glycoprotein B (gB) gene specifying gB derivatives lacking the carboxy-terminal 28, 49, or 64 amino acids. A complementation system that utilized gBs expressed in COS cells to complement gB-null virus K delta T was esta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2396-2401.1993
更新日期:1993-04-01 00:00:00
abstract::A receptor on YAC-1 cells, a mouse T-lymphoma cell line, bound all six serotypes of the group B coxsackieviruses (CVB). In addition, the cells produced infectious virus. Each of the CVB competed for the same receptor on YAC-1 cells. CVB3 bound relatively slowly to YAC-1 cells (k = 4 x 10(-11) min-1 cell-1), and there ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3105-3108.1989
更新日期:1989-07-01 00:00:00
abstract:UNLABELLED:Several innate sensing pathways contribute to the control of early cytomegalovirus (CMV) infection, leading to a multiphasic type I interferon (IFN-I) response that limits viral replication and promotes host defenses. Toll-like receptor (TLR)-dependent pathways induce IFN-I production in CMV-infected plasmac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01040-16
更新日期:2016-08-12 00:00:00
abstract::The existence of novel endogenous retrovirus elements in the chicken genome, designated EAV-HP, with close sequence identity to the env gene of avian leukosis virus (ALV) subgroup J has been reported (L. M. Smith, A. A. Toye, K. Howes, N. Bumstead, L. N. Payne, and K. Venugopal, J. Gen. Virol. 80:261-268, 1999). To re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.3.1296-1306.2000
更新日期:2000-02-01 00:00:00
abstract::The Nef proteins of human immunodeficiency virus and simian immunodeficiency virus (SIV) bind the AP-1 and AP-2 clathrin adaptors to downmodulate the expression of CD4 and CD28 by recruiting them to sites of AP-2 clathrin-dependent endocytosis. Additionally, SIV Nef directly binds the CD3-zeta subunit of the CD3 compl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.14.8116-8126.2003
更新日期:2003-07-01 00:00:00
abstract::Immune responses to vector-corrected cells have limited the application of gene therapy for treatment of chronic disorders such as inherited deficiency states. We have found that recombinant adeno-associated virus (AAV) efficiently transduces muscle fibers in vivo without activation of cellular and humoral immunity to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4212-4223.1998
更新日期:1998-05-01 00:00:00
abstract::Progressive multifocal leukoencephalopathy (PML) is an often-fatal demyelinating disease of the central nervous system. PML results when oligodendrocytes within immunocompromised individuals are infected with the human JC virus (JCV). We have identified an oligodendrocyte precursor cell line, termed G144, that support...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00735-17
更新日期:2017-09-27 00:00:00
abstract:UNLABELLED:The RNA genome of respiratory syncytial virus (RSV) is constitutively encapsidated by the viral nucleoprotein N, thus forming a helical nucleocapsid. Polymerization of N along the genomic and antigenomic RNAs is concomitant to replication and requires the preservation of an unassembled monomeric nucleoprotei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03666-14
更新日期:2015-04-01 00:00:00
abstract::The cellular tropism of the feline immunodeficiency virus (FIV) is affected by changes in variable region 3 (V3) of the surface (SU) envelope glycoprotein (Verschoor, E. J., et al., J. Virol. 69:4752-4757, 1995). By using high-dose DNA transfection, an FIV molecular clone with a non-CRFK-tropic V3 acquired the ability...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.7132-7135.1997
更新日期:1997-09-01 00:00:00
abstract:UNLABELLED:Respiratory syncytial virus (RSV) is the most important cause of lower respiratory tract infection in young children and the leading cause of infant hospitalization worldwide. Uncontrolled response to RSV is mediated by a toll-like receptor (TLR)-mediated immune response. Resveratrol possesses anti-RSV activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03637-13
更新日期:2014-04-01 00:00:00
abstract::Herpes simplex virus type 2 (HSV-2) glycoprotein B (gB-2) gene segments were expressed as recombinant proteins in Escherichia coli. gB-2 recombinant proteins were reacted with human serum immunoglobulin G (IgG) antibodies in Western immunoblot assays. Initially, samples were tested for the presence of HSV-1-specific a...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.70.5.2950-2956.1996
更新日期:1996-05-01 00:00:00