Abstract:
:Previous analyses using in situ hybridization alone or together with PCR have yielded conflicting results regarding the cell type in which latent varicella-zoster virus (VZV) resides. We separated human trigeminal ganglia (TG) into neuronal and nonneuronal fractions, followed by primary and nested PCR to quantitate VZV DNA at the single cell level. Both TG from each of eight cadavers were dissociated and separated into neuronal and nonneuronal cell suspensions by differential filtration. Analysis of the neuron fraction (5,000 neurons per sample) revealed VZV DNA in 9 of 16 samples, with copy numbers ranging from 1 to 12, whereas only 2 of 16 nonneuronal cell samples were positive for VZV DNA, with 1 copy each. Further analysis of 10 samples of 100 neurons and the corresponding nonneuronal cell fractions from each TG of a single subject revealed VZV DNA in 3 of 10 samples of the left TG (range, 2 to 5 copies) and in 1 of 10 samples of the right TG (2 copies) but in none of the 20 nonneuronal cell fractions. These data indicate that latent VZV DNA is present primarily, if not exclusively, in neurons, at a frequency of two to five copies per latently infected neuron.
journal_name
J Viroljournal_title
Journal of virologyauthors
LaGuardia JJ,Cohrs RJ,Gilden DHdoi
10.1128/JVI.73.10.8571-8577.1999subject
Has Abstractpub_date
1999-10-01 00:00:00pages
8571-7issue
10eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::During secondary immune responses to influenza virus, virus-specific T memory cells are a major source of gamma interferon (IFN-gamma). We assessed the contribution of IFN-gamma to heterologous protection against the A/WSN/33 (H1N1) virus of wild-type and IFN-gamma-/- mice previously immunized with the A/HK/68 (H3N2) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6637-6645.1998
更新日期:1998-08-01 00:00:00
abstract::The Kresse strain of porcine parvovirus (PPV) was cloned into pUC19, and independent infectious clones were sequenced. The PPV Kresse and NADL-2 strains, which have different pathogenicities, shared an identical genomic organization and a high degree of sequence identity. Partial genomes (1.5 or 1.6 kb) of 15 field is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2508-2515.1996
更新日期:1996-04-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection is a major cause of liver disease. The HCV polyprotein contains a hypervariable region (HVR1) located at the N terminus of the second envelope glycoprotein E2. The strong variability of this 27-amino-acid region is due to its apparent tolerance of amino acid substitutions toge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5703-5710.2001
更新日期:2001-06-01 00:00:00
abstract::Endocytosis and endosomal trafficking regulate the proteins targeted to the plasma membrane and play essential roles in diverse cellular processes, including responses to pathogen attack. Here, we report the identification of Glycine max (soybean) endocytosis dynamin-like protein 5A (GmSDL5A) associated with purified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01320-18
更新日期:2018-11-12 00:00:00
abstract::The three primary capsid proteins (A, B, and C) of adeno-associated viruses have been shown previously to contain overlapping amino acid sequences (R. McPherson and J. Rose, J. Virol. 46:523-529, 1983). In the present study we demonstrate definitively that these proteins are encoded in the right half of the adeno-asso...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.591-597.1984
更新日期:1984-11-01 00:00:00
abstract::Murine gammaherpesvirus 68 (gammaHV68) provides an important experimental model for understanding mechanisms of immune control of the latent human gammaherpesviruses. Antiviral CD8 T cells play a key role throughout three separate phases of the infection: clearance of lytic virus, control of the latency amplification ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02229-09
更新日期:2010-03-01 00:00:00
abstract::The retroviral primary transcription product is a multifunctional RNA that is utilized as pre-mRNA, mRNA, and genomic RNA. The relationship between human immunodeficiency virus type 1 (HIV-1) unspliced transcripts used as mRNA for viral protein synthesis and as virion precursor RNA (vpRNA) for encapsidation remains an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11531-11537.2000
更新日期:2000-12-01 00:00:00
abstract::Rift Valley fever virus strain MP-12 was generated by serial plaque passages of parental strain ZH548 12 times in MRC-5 cells in the presence of a chemical mutagen, 5-fluorouracil. As a result, MP-12 encoded 4, 9, and 10 mutations in the S, M, and L segments, respectively. Among them, mutations in the M and L segments...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02081-12
更新日期:2012-12-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is a prevalent pathogen that establishes lifelong infection in the host. Virus persistence is aided by extensive manipulation of the host immune system, particularly cytokine and chemokine signaling pathways. The HCMV UL111A gene encodes cmvIL-10, an ortholog of human interleukin-10 that h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01981-17
更新日期:2018-02-12 00:00:00
abstract::The complete genome sequences of the human coronavirus OC43 (HCoV-OC43) laboratory strain from the American Type Culture Collection (ATCC), and a HCoV-OC43 clinical isolate, designated Paris, were obtained. Both genomes are 30,713 nucleotides long, excluding the poly(A) tail, and only differ by 6 nucleotides. These si...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8824-8834.2004
更新日期:2004-08-01 00:00:00
abstract::Egress of wrapped virus (WV) to the cell periphery following vaccinia virus (VACV) replication is dependent on interactions with the microtubule motor complex kinesin-1 and is mediated by the viral envelope protein A36. Here we report that ectromelia virus (ECTV), a related orthopoxvirus and the causative agent of mou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06636-11
更新日期:2012-07-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection is associated with impaired proliferative, cytokine, and cytotoxic effector functions of HCV-specific CD8(+) T cells that probably contribute significantly to viral persistence. Here, we investigated the potential role of T cells with a CD4(+)CD25(+) regulatory phenotype in su...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7860-7867.2005
更新日期:2005-06-01 00:00:00
abstract::Human norovirus (HuNoV) is a major causative agent of food-borne gastroenteritis worldwide. Currently, there are no vaccines or effective therapeutic interventions for this virus. Development of an attenuated vaccine for HuNoV has been hampered by the inability to grow the virus in cell culture. Thus, a vector-based v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02332-10
更新日期:2011-03-01 00:00:00
abstract::A method to study the function of individual African swine fever virus (ASFV) gene products utilizing the Escherichia coli lac repressor-operator system has been developed. Recombinant viruses containing both the lacI gene encoding the lac repressor and a strong virus late promoter modified by the insertion of one or ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3185-3195.1998
更新日期:1998-04-01 00:00:00
abstract::Injection of conventional or axenic weanling mice with potent sheep or goat antibody to mouse interferon alpha/beta resulted in a decrease in the basal level of 2-5A synthetase in resting peritoneal macrophages and rendered these cells permissive for vesicular stomatitis virus. There was a good inverse correlation bet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.1.221-227.1985
更新日期:1985-01-01 00:00:00
abstract::A human cell line (293) transformed by adenovirus type 5 encodes mRNA's and proteins from the early region 1 (E1) of the viral genome. These products correspond to those synthesized early after adenovirus infection of normal cells. This pattern of expression is different from that observed at later times in the lytic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.3.860-871.1980
更新日期:1980-12-01 00:00:00
abstract::We have developed a high-throughput sequencing (HTS) workflow for investigating paramyxovirus transcription and replication. We show that sequencing of oligo(dT)-selected polyadenylated mRNAs, without considering the orientation of the RNAs from which they had been generated, cannot accurately be used to analyze the a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00571-19
更新日期:2019-08-13 00:00:00
abstract::The folded genome of Escherichia coli is converted to a slower-sedimenting form within 5 min after infection with bacteriophage T4 or T4nd28(den A)-amN82(44). Chloramphenicol sensitivity and response to UV-irradiation of the phage suggest participation of viral-induced functions. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.548-550.1974
更新日期:1974-02-01 00:00:00
abstract::Human T-cell lymphotropic virus type 1 (HTLV-1) is transmitted through a viral synapse and enters target cells via interaction with the glucose transporter GLUT1. Here, we show that Neuropilin-1 (NRP1), the receptor for semaphorin-3A and VEGF-A165 and a member of the immune synapse, is also a physical and functional p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02719-05
更新日期:2006-07-01 00:00:00
abstract::Assembly and maturation of retroviral particles requires the aggregation and controlled proteolytic cleavage of polyprotein core precursors by a precursor-encoded protease (PR). Active, mature retroviral PR is a dimer, and the accumulation of precursors at sites of assembly may facilitate subunit interaction and subse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6165-6172.1991
更新日期:1991-11-01 00:00:00
abstract::During studies of APOBEC3 (A3) anti-human immunodeficiency virus type 1 (anti-HIV-1) mechanisms, we identified a single cysteine at position 320 (C320) that disrupts A3DE activity. This residue is located in the recently identified DNA binding domain in A3G. Replacing C320 with a corresponding tyrosine from A3F (Y307)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00243-11
更新日期:2011-06-01 00:00:00
abstract::The viral membrane proteins M and E are the minimal requirements for the budding of coronavirus particles. Since the E protein occurs in particles only in trace amounts, the lateral interactions between the M proteins apparently generate the major driving force for envelope formation. By using coimmunoprecipitation an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.4967-4978.2000
更新日期:2000-06-01 00:00:00
abstract::The therapeutic efficacy of direct drug infusion into the brain, the target organ of transmissible spongiform encephalopathies, was assessed in transgenic mice intracerebrally infected with 263K scrapie agent. Pentosan polysulfate (PPS) gave the most dramatic prolongation of the incubation period, and amphotericin B h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.10.4999-5006.2004
更新日期:2004-05-01 00:00:00
abstract::Marek's disease virus DNA appears to be wound around a central structure connecting the two inner poles of the capsid. Based on electron micrographs of Marek's disease virions, a diagram of spatial configuration of virus DNA is presented. This diagram may explain the pleomorphic character of the herpesvirus core obser...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1148-1150.1974
更新日期:1974-05-01 00:00:00
abstract::Antibody was raised against purified vaccinia virus RNA polymerase and used to screen a recombinant vaccinia virus-lambda gt11 library. The DNA from several immunopositive clones was shown by Southern hybridization to originate from the vaccinia virus HindIII E fragment. The nucleotide sequence of the RNA polymerase s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5376-5382.1990
更新日期:1990-11-01 00:00:00
abstract::The mechanism of cellular src (c-src) transduction by a transformation-defective deletion mutant, td109, of Rous sarcoma virus was studied by sequence analysis of the recombinational junctions in three td109-derived recovered sarcoma viruses (rASVs). Our results show that two rASVs have been generated by recombination...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.3.556-563.1986
更新日期:1986-09-01 00:00:00
abstract::A DNA vaccine plasmid containing the Japanese encephalitis (JE) virus premembrane (prM) and envelope (E) genes (designated pcDNA3JEME) was evaluated for immunogenicity and protective efficacy in mice. Two immunizations of 4-week-old female ICR mice with pcDNA3JEME by intramuscular or intradermal injections at a dose o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4925-4930.1998
更新日期:1998-06-01 00:00:00
abstract::The coronavirus, mouse hepatitis virus strain JHM, causes acute and chronic neurological diseases in rodents. Here we demonstrate that two closely related virus variants, both of which cause acute encephalitis in susceptible strains of mice, cause markedly different diseases if mice are protected with a suboptimal amo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10260-10269.2003
更新日期:2003-10-01 00:00:00
abstract::After rabies virus glycoprotein was treated with CNBr, the peptide mixture was fractionated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CNBr-cleaved peptide fragments were resolved into seven peptide bands under reducing conditions and six peptide bands under nonreducing conditions. The i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.595-602.1982
更新日期:1982-11-01 00:00:00
abstract::Substitution of a methionine residue at position 79 in poliovirus protein 3A with valine or threonine caused defective viral RNA synthesis, manifested as delayed onset and reduced yield of viral RNA, in HeLa cells transfected with a luciferase-containing replicon. Viruses containing these same mutations produced small...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12679-12691.2003
更新日期:2003-12-01 00:00:00