Abstract:
:Immune responses to vector-corrected cells have limited the application of gene therapy for treatment of chronic disorders such as inherited deficiency states. We have found that recombinant adeno-associated virus (AAV) efficiently transduces muscle fibers in vivo without activation of cellular and humoral immunity to neoantigenic transgene products such as beta-galactosidase, which differs from the experience with recombinant adenovirus, where vibrant T-cell responses to the transgene product destroy the targeted muscle fibers. T cells activated following intramuscular administration of adenovirus expressing lacZ (AdlacZ) can destroy AAVlacZ-transduced muscle fibers, indicating a prior state of immunologic nonresponsiveness in the context of AAV gene therapy. Adoptive transfer of dendritic cells infected with AdlacZ leads to immune mediated elimination of AAVlacZ-transduced muscle fibers. AAVlacZ-transduced antigen-presenting cells fail to demonstrate beta-galactosidase activity and are unable to elicit transgene immunity in adoptive transfer experiments. These studies indicate that vector-mediated transduction of dendritic cells is necessary for cellular immune responses to muscle gene therapy, a step which AAV avoids, providing a useful biological niche for its use in gene therapy.
journal_name
J Viroljournal_title
Journal of virologyauthors
Jooss K,Yang Y,Fisher KJ,Wilson JMdoi
10.1128/JVI.72.5.4212-4223.1998subject
Has Abstractpub_date
1998-05-01 00:00:00pages
4212-23issue
5eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::Adeno-associated virus (AAV) integrates very efficiently into a specific site (AAVS1) of human chromosome 19. Two elements of the AAV genome are sufficient: the inverted terminal repeats (ITRs) and the Rep78 or Rep68 protein. The incorporation of the AAV integration machinery in nonviral delivery systems is of great i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7653-7658.1998
更新日期:1998-09-01 00:00:00
abstract::H5N1 highly pathogenic avian influenza virus (HPAIV) has continued to spread and poses a significant threat to both animal and human health. Current influenza vaccine strategies have limitations that prevent their effective use for widespread inoculation of animals in the field. Vaccine strains of Newcastle disease vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01514-06
更新日期:2007-01-01 00:00:00
abstract::Minimal transformants of rat F111 fibroblasts were established after infection with the large T antigen (large T)-encoding retroviral expression vector pZIPTEX (M. Brown, M. McCormack, K. Zinn, M. Farrell, I. Bikel, and D. Livingston, J. Virol. 60:290-293, 1986). Coexpression of small t antigen (small t) in these cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1555-1563.1993
更新日期:1993-03-01 00:00:00
abstract::Reactivations of persistent viral infections pose a significant medical problem in immunocompromised cancer, transplant, and AIDS patients, yet little is known about how persistent viral infections are immunologically controlled. Here we describe a mouse model for investigating the role of the immune response in contr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6559-6564.1998
更新日期:1998-08-01 00:00:00
abstract::Suppression of interferon signaling is of paramount importance to a virus. Interferon signaling significantly reduces or halts the ability of a virus to replicate; therefore, viruses have evolved sophisticated mechanisms that suppress activation of the interferon pathway or responsiveness of the infected cell to inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-16
更新日期:2017-03-29 00:00:00
abstract::We used reovirus reassortant genetics and severe combined immunodeficient (SCID) mice to define viral genes important for organ tropism and virulence in the absence of antigen-specific immunity. Adult SCID mice infected with reovirus serotype 1 strain Lang (T1L) died after 20 +/- 6 days, while infection with serotype ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.357-364.1995
更新日期:1995-01-01 00:00:00
abstract::Two different proteins with high affinities for the pteridine ring of folic acid have been used to determine the location of this portion of the folate molecule in the tail plate of T4D and other T-even bacteriophage particles. The two proteins used were (i) antibody specific for folic acid and (ii) the folate-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.6.1391-1400.1975
更新日期:1975-12-01 00:00:00
abstract::Adeno-associated virus (AAV) is gaining momentum as a gene therapy vector for human applications. However, there remain impediments to the development of this virus as a vector. One of these is the incomplete understanding of the biology of the virus, including nuclear targeting of the incoming virion during initial i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02723-05
更新日期:2006-06-01 00:00:00
abstract::Two chimpanzees immunized with woodchuck hepatitis virus (WHV) surface antigen (WHsAg) developed antibodies cross-reactive with hepatitis B virus (HBV) surface antigen (HBsAg). After challenge with HBV, one animal was completely protected and the other experienced a subclinical infection, without evidence of liver dis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.895-901.1986
更新日期:1986-12-01 00:00:00
abstract::Oligonucleotide-directed mutagenesis was used to construct a nonsense mutation in open reading frame (ORF) E2 of bovine papillomavirus DNA. A single base substitution mutation was constructed which converted a TAC codon into a TAG amber stop codon at a position in the ORF that did not overlap with any other viral ORFs...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.2.475-480.1986
更新日期:1986-02-01 00:00:00
abstract::The majority of HIV-1 infections around the world result from non-B clade HIV-1 strains. The CRF01_AE (AE) strain is seen principally in Southeast Asia. AE protease differs by approximately 10% in amino acid sequence from clade B protease and carries several naturally occurring polymorphisms that are associated with d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00505-10
更新日期:2010-10-01 00:00:00
abstract::Dengue virus (DENV) is a major global pathogen that causes significant morbidity and mortality in tropical and subtropical areas worldwide. An improved understanding of the regions within the DENV genome and its encoded proteins that are required for the virus replication cycle will expedite the development of urgentl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01455-17
更新日期:2017-11-14 00:00:00
abstract::The structural proteins of mature LPP-1 particles and the patterns of protein synthesis after LPP-1 infection have been examined by electrophoresis on sodium dodecyl sulfate polyacrylamide gels. Structural proteins account for 35% of the LPP-1 genome, and proteins that would require about 65% of the total coding capac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.6.841-846.1970
更新日期:1970-12-01 00:00:00
abstract::Simian virus 40 (SV40)-transformed human cells (LN-SV) were fused with BALB/c peritoneal macrophages (BALB/c X LN-SV) and with C57BL peritoneal macrophages (C57BL X LN-SV) and hybrid clones, all of which had segregated human chromosomes and contained the entire complement of mouse chromosomes, were isolated. All 15 BA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.1143-1146.1976
更新日期:1976-06-01 00:00:00
abstract::Two herpes simplex virus type 1 glycoproteins, gE and gI, have been shown to form a complex that binds the Fc domain of immunoglobulin G (IgG). We demonstrate that this complex is required for the binding of monomeric nonimmune IgG but that gE alone is sufficient for binding polymeric IgG in the form of IgG complexes....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2725-2731.1990
更新日期:1990-06-01 00:00:00
abstract::Cauliflower mosaic virus (CaMV) forms two types of inclusion bodies within infected plant cells: numerous virus factories, which are the sites for viral replication and virion assembly, and a single transmission body (TB), which is specialized for virus transmission by aphid vectors. The TB reacts within seconds to ap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01883-13
更新日期:2013-11-01 00:00:00
abstract::We report here the complete genomic sequence of the duck Tembusu virus (DTMUV) WJ-1 strain, isolated from Muscovy ducks. This is the first complete genome sequence of DTMUV reported in southern China. Compared with the other strains (TA, GH-2, YY5, and ZJ-407) that were previously found in eastern China, WJ-1 bears a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02361-12
更新日期:2012-12-01 00:00:00
abstract::The E4orf6 protein of group C adenovirus is an oncoprotein that, in association with the E1B 55-kDa protein and by E1B-independent means, promotes virus replication. An arginine-faced amphipathic alpha-helix in the E4orf6 protein is required for the E4orf6 protein to direct nuclear localization of the E1B 55-kDa prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1475-1487.2002
更新日期:2002-02-01 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus (HIV) seizes control of cellular cullin-RING E3 ubiquitin ligases (CRLs) to promote viral replication. HIV-1 Vpr and HIV-2/simian immunodeficiency virus (SIV) Vpr and Vpx engage the cullin4 (CUL4)-containing ubiquitin ligase complex (CRL4) to cause polyubiquitination and proteaso...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00241-14
更新日期:2014-06-01 00:00:00
abstract::The initiation of RNA transcription by the virion-bound RNA transcriptase of vesicular stomatitis virus has been examined. Multiple initiation sequences have been observed, two of which have been characterized (pppApCpGp... and pppGpCp...) suggestive of a transcription process which can start at different sites along ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.4.487-501.1973
更新日期:1973-04-01 00:00:00
abstract::Initiation of herpesvirus infection requires attachment of virions to the host cell followed by fusion of virion envelope and cellular cytoplasmic membrane during penetration. In several alphaherpesviruses, glycoprotein C (gC) is the primary attachment protein, interacting with cell-surface heparan sulfate proteoglyca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7341-7348.1998
更新日期:1998-09-01 00:00:00
abstract::To examine the pathway of the coreceptor switching of CCR5-using (R5) virus to CXCR4-using (X4) virus in simian-human immunodeficiency virus SHIV(SF162P3N)-infected rhesus macaque BR24, analysis was performed on variants present at 20 weeks postinfection, the time when the signature gp120 V3 loop sequence of the X4 sw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00570-08
更新日期:2008-07-01 00:00:00
abstract::While RNA silencing is a potent antiviral defense in plants, well-adapted plant viruses are known to encode suppressors of RNA silencing (VSR) that can neutralize the effectiveness of RNA silencing. As a result, most plant genes involved in antiviral silencing were identified by using debilitated viruses lacking silen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00497-12
更新日期:2012-06-01 00:00:00
abstract::The adenovirus fiber protein is used for attachment of the virus to a specific receptor on the cell surface. Structurally, the protein consists of a long, thin shaft that protrudes from the vertex of the virus capsid and terminates in a globular domain termed the knob. To verify that the knob is the domain which inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5239-5246.1994
更新日期:1994-08-01 00:00:00
abstract::Infectious poxvirus particles are unusual in that they are brick shaped and lack symmetry. Nevertheless, an external honeycomb lattice comprised of a capsid-like protein dictates the spherical shape and size of immature poxvirus particles. In the case of vaccinia virus, trimers of 63-kDa D13 polypeptides form the buil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00875-09
更新日期:2009-09-01 00:00:00
abstract::Purified Friend viruses obtained from chronically infected tissue cultures were studied under the electron microscope in an effort to correlate the fine structure of the particles to their leukemogenic activity under varied experimental conditions, i.e., temperature treatments and exposure to Tween 80, amyl acetate, o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.4.365-375.1968
更新日期:1968-04-01 00:00:00
abstract::The production and selection of infectious vaccinia virus recombinants expressing foreign genes was facilitated by the construction of plasmid vectors. These vectors contain all or part of the vaccinia virus thymidine kinase (TK) gene interrupted by multiple unique restriction endonuclease sites placed adjacent to the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.857-864.1984
更新日期:1984-03-01 00:00:00
abstract::In order to explore the potential of retrovirus vectors for efficiently transferring foreign genes into mouse embryos, a replication-competent recombinant Moloney murine leukemia virus (Mo-MLV) vector carrying a mutant dihydrofolate reductase (DHFR) cDNA insert in the U3 region of the viral long terminal repeat was us...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4857-4865.1989
更新日期:1989-11-01 00:00:00
abstract::The second of three short upstream open reading frames (uORF2) in the transcript leader of the human cytomegalovirus gp48 (gpUL4) virion glycoprotein gene inhibits downstream translation approximately 10-fold. Remarkably, this inhibition depends on the amino acid coding information of uORF2. In the current studies we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1030-1036.1995
更新日期:1995-02-01 00:00:00
abstract::Human norovirus (HuNoV) is a major causative agent of food-borne gastroenteritis worldwide. Currently, there are no vaccines or effective therapeutic interventions for this virus. Development of an attenuated vaccine for HuNoV has been hampered by the inability to grow the virus in cell culture. Thus, a vector-based v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02332-10
更新日期:2011-03-01 00:00:00