Abstract:
:Senecavirus A (SVA) is a picornavirus that causes acute vesicular disease (VD), that is clinically indistinguishable from foot-and-mouth disease (FMD), in pigs. Notably, SVA RNA has been detected in lymphoid tissues of infected animals several weeks following resolution of the clinical disease, suggesting that the virus may persist in select host tissues. Here, we investigated the occurrence of persistent SVA infection and the contribution of stressors (transportation, immunosuppression, or parturition) to acute disease and recrudescence from persistent SVA infection. Our results show that transportation stress leads to a slight increase in disease severity following infection. During persistence, transportation, immunosuppression, and parturition stressors did not lead to overt/recrudescent clinical disease, but intermittent viremia and virus shedding were detected up to day 60 postinfection (p.i.) in all treatment groups following stress stimulation. Notably, real-time PCR and in situ hybridization (ISH) assays confirmed that the tonsil harbors SVA RNA during the persistent phase of infection. Immunofluorescence assays (IFA) specific for double-stranded RNA (dsRNA) demonstrated the presence of double-stranded viral RNA in tonsillar cells. Most importantly, infectious SVA was isolated from the tonsil of two animals on day 60 p.i., confirming the occurrence of carrier animals following SVA infection. These findings were supported by the fact that contact piglets (11/44) born to persistently infected sows were infected by SVA, demonstrating successful transmission of the virus from carrier sows to contact piglets. Results here confirm the establishment of persistent infection by SVA and demonstrate successful transmission of the virus from persistently infected animals.IMPORTANCE Persistent viral infections have significant implications for disease control strategies. Previous studies demonstrated the persistence of SVA RNA in the tonsil of experimentally or naturally infected animals long after resolution of the clinical disease. Here, we showed that SVA establishes persistent infection in SVA-infected animals, with the tonsil serving as one of the sites of virus persistence. Importantly, persistently infected carrier animals shedding SVA in oral and nasal secretions or feces can serve as sources of infection to other susceptible animals, as evidenced by successful transmission of SVA from persistently infected sows to contact piglets. These findings unveil an important aspect of SVA infection biology, suggesting that persistently infected pigs may function as reservoirs for SVA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Maggioli MF,Fernandes MHV,Joshi LR,Sharma B,Tweet MM,Noll JCG,Bauermann FV,Diel DGdoi
10.1128/JVI.00819-19subject
Has Abstractpub_date
2019-10-15 00:00:00issue
21eissn
0022-538Xissn
1098-5514pii
JVI.00819-19journal_volume
93pub_type
杂志文章abstract::Some paramyxovirus V proteins induce STAT protein degradation, and the amino acids essential for this process in the human parainfluenza virus type 2 (hPIV2) V protein have been studied. Various recombinant hPIV2s and cell lines constitutively expressing various mutant V proteins were generated. We found that V protei...
journal_title:Journal of virology
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journal_title:Journal of virology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1972-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00959-06
更新日期:2006-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4115-4122.1990
更新日期:1990-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.4705-4709.1992
更新日期:1992-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2395-2406.1987
更新日期:1987-08-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
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更新日期:1994-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1984-08-01 00:00:00
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journal_title:Journal of virology
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更新日期:2019-05-01 00:00:00
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journal_title:Journal of virology
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更新日期:2003-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,收录出版
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更新日期:2002-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.2.697-706.2002
更新日期:2002-01-01 00:00:00
abstract::The antigenic binding sites of two monoclonal antibodies are located in the COOH-terminal region (clone 412) and probably in an internal region (clone 7) of simian virus 40 large T antigen. A third monoclonal antibody (clone 122), which has been shown to bind nonviral T antigen, does not react with HeLa cells infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.478-482.1981
更新日期:1981-01-01 00:00:00