Abstract:
:After entering a host cell, retroviruses such as simian immunodeficiency virus (SIV) uncoat, disassembling the viral capsid. Rates of uncoating that are too high and too low can be detrimental to the efficiency of infection. Rapid uncoating typically leads to blocks in reverse transcription, but the basis for replication defects associated with slow uncoating is less clear. Here we characterize the phenotypes of two SIVmac239 mutants with changes, A87E and A87D, in the helix 4/5 loop of the capsid protein. These mutant viruses exhibited normal capsid morphology but were significantly attenuated for infectivity. The infectivity of wild-type and mutant SIVmac239 was not decreased by aphidicolin-induced growth arrest of the target cells. In the cytosol of infected cells, the A87E and A87D capsids remained in particulate form longer than the wild-type SIVmac239 capsid, suggesting that the mutants uncoat more slowly than the wild-type capsid. Both mutants exhibited much higher levels of autointegrated DNA forms than wild-type SIVmac239. Thus, some changes in the helix 4/5 loop of the SIVmac239 capsid protein result in capsid hyperstability and an increase in autointegration.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tipper C,Sodroski Jdoi
10.1128/JVI.03239-12subject
Has Abstractpub_date
2013-04-01 00:00:00pages
3628-39issue
7eissn
0022-538Xissn
1098-5514pii
JVI.03239-12journal_volume
87pub_type
杂志文章abstract::CTCF and the cohesin complex modify chromatin by binding to DNA and interacting with each other and with other cellular proteins. Both proteins regulate transcription by a variety of local effects on transcription and by long-range topological effects. CTCF and cohesin also bind to herpesvirus genomes at specific site...
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1976-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-04-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.68.1.548-554.1994
更新日期:1994-01-01 00:00:00