Abstract:
:In order to study cell tropism and attenuation of hepatitis A virus (HAV), the genome of HAV wild-type GBM and two cell culture-adapted variants, GBM/FRhK and GBM/HFS, were cloned and sequenced after amplification by reverse transcriptase-PCR. During virus cultivation, the HAV variant GBM/FRhK had a strict host range for FRhK-4 cells, in contrast to GBM/HFS, which can be grown in HFS and FRhK-4 cells. The HAV variant GBM/HFS was shown to be attenuated when inoculated into chimpanzees (B. Flehmig, R. F. Mauler, G. Noll, E. Weinmann, and J. P. Gregerson, p. 87-90, in A. Zuckerman, ed., Viral Hepatitis and Liver Disease, 1988). On the basis of this biological background, the comparison of the nucleotide sequences of these three HAV GBM variants should elucidate differences which may be of importance for cell tropism and attenuation. The comparison of the genome between the GBM wild type and HAV wild types HM175 (J. I. Cohen, J. R. Ticehurst, R. H. Purcell, A. Buckler-White, and B. M. Baroudy, J. Virol. 61:50-59, 1987) and HAV-LA (R. Najarian, O. Caput, W. Gee, S. J. Potter, A. Renard, J. Merryweather, G. Van Nest, and D. Dina, Proc. Natl. Acad. Sci. USA 82:2627-2631, 1985) showed a 92 to 96.3% identity, whereas the identity was 99.3 to 99.6% between the GBM variants. Nucleotide differences between the wild-type and the cell culture-adapted variants, which were identical in both cell culture-adapted GBM variants, were localized in the 5' noncoding region; in 2B, 3B, and 3D; and in the 3' noncoding region. Our result concerning the 2B/2C region confirms a mutation at position 3889 (C-->T, alanine to valine), which had been shown to be of importance for cell culture adaptation (S. U. Emerson, C. McRill, B. Rosenblum, S. M. Feinstone, and R. H. Purcell, J. Virol. 65:4882-4886, 1991; S. U. Emerson, Y. K. Huang, C. McRill, M. Lewis, and R. H. Purcell, J. Virol. 66:650-654, 1992), whereas other mutations differ from published HAV sequence data and may be cell specific. Further comparison of the two cell culture-adapted GBM variants showed cell-specific mutations resulting in deletions of six amino acids in the VP1 region and three amino acids in the 3A region of the GBM variant GBM/FRhK.
journal_name
J Viroljournal_title
Journal of virologyauthors
Graff J,Normann A,Feinstone SM,Flehmig Bdoi
10.1128/JVI.68.1.548-554.1994subject
Has Abstractpub_date
1994-01-01 00:00:00pages
548-54issue
1eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::The structure and replication of a cold-adapted, temperature-sensitive (TS) mutant of an Asian (H2N2) influenza virus was compared with that of its wild-type (WT) parent. Viruses were grown in a chicken kidney cell system, and at the nonpermissive temperature of 40 C, production of infectious TS virus was about 100,00...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1503-1511.1973
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abstract:UNLABELLED:In the last decade, novel tick-borne pathogenic phleboviruses in the family Bunyaviridae, all closely related to Uukuniemi virus (UUKV), have emerged on different continents. To reproduce the tick-mammal switch in vitro, we first established a reverse genetics system to rescue UUKV with a genome close to tha...
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doi:10.1128/JVI.63.8.3353-3361.1989
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abstract::The DNAs of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses contain overlapping segments of the early region of wild-type SV40 DNA. The complementary DNA strands of these five viruses have been separated with synthetic polyribonucleotides in isopycnic cesium chloride gradients. The relat...
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pub_type: 杂志文章
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abstract::Rift Valley fever virus (RVFV), a phlebovirus of the family Bunyaviridae, is a major public health threat in Egypt and sub-Saharan Africa. The viral and host cellular factors that contribute to RVFV virulence and pathogenicity are still poorly understood. All pathogenic RVFV strains direct the synthesis of a nonstruct...
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abstract::Longitudinal studies of T cell immune responses during viral infections in humans are essential for our understanding of how effector T cell responses develop, clear infection, and provide long-lasting immunity. Here, following an outbreak of a Puumala hantavirus infection in the human population, we longitudinally an...
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pub_type: 杂志文章
doi:10.1128/JVI.05548-11
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pub_type: 杂志文章
doi:10.1128/JVI.63.4.1803-1807.1989
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pub_type: 杂志文章
doi:10.1128/JVI.61.4.1244-1247.1987
更新日期:1987-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.25.1.60-72.1978
更新日期:1978-01-01 00:00:00
abstract:UNLABELLED:Hepatitis C virus (HCV) infects 180 million people worldwide and is a leading cause of liver diseases such as fibrosis, cirrhosis, and hepatocellular carcinoma. It has been shown that HCV can spread to naive cells using two distinct entry mechanisms, "cell-free" entry of infectious extracellular virions that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03241-13
更新日期:2014-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00040-09
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00272-10
更新日期:2010-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3470-3477.2000
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7868-7873.2002
更新日期:2002-08-01 00:00:00
abstract::The RNA genome of human immunodeficiency virus type 1 (HIV-1) is converted into DNA after infection in order to integrate into the host cell DNA. However, a large number of these reverse-transcribed genomes remain unintegrated in the nucleus of infected cells. Currently, there are no data available about the intranucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7683-7691.2001
更新日期:2001-08-01 00:00:00
abstract::The use of different viral promoters for the expression of the EBNA1 gene product appears to be a critical step in the regulation of Epstein-Barr virus latent gene expression and may reflect the extent of differentiation of B-cell hosts. Low-passage Burkitt lymphoma cell lines resemble immature B cells in that they ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6421-6431.1994
更新日期:1994-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.7956-7967.2002
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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doi:10.1128/JVI.28.1.344-351.1978
更新日期:1978-10-01 00:00:00
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journal_title:Journal of virology
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更新日期:2007-09-01 00:00:00
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更新日期:1977-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.78.23.12987-12995.2004
更新日期:2004-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1268-1273.1974
更新日期:1974-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2755-2761.1988
更新日期:1988-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1986-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.1.17-24.1969
更新日期:1969-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4351-4360.2000
更新日期:2000-05-01 00:00:00
abstract::We report the use of a cDNA microarray to monitor global transcriptional responses of the chestnut blight fungus, Cryphonectria parasitica, to infection by mild and severe isolates of virulence-attenuating hypoviruses that share 87 to 93% and 90 to 98% identity at the nucleotide and amino acid levels, respectively. In...
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pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00