Abstract:
:Human immunodeficiency virus type 1 uses ribosomal frameshifting for translation of the Gag-Pol polyprotein. Frameshift activities are thought to be tightly regulated. Analysis of gag p1 sequences from 270 plasma virions identified in 64% of the samples the occurrence of polymorphism that could lead to changes in thermodynamic stability of the stem-loop. Expression in Saccharomyces cerevisiae of p1-beta-galactosidase fusion proteins from 10 representative natural stem-loop variants and three laboratory mutant constructs (predicted the thermodynamic stability [Delta G degrees] ranging from -23.0 to -4.3 kcal/mol) identified a reduction in frameshift activity of 13 to 67% compared with constructs with the wild-type stem-loop (Delta G degrees, -23.5 kcal/mol). Viruses carrying stem-loops associated with greater than 60% reductions in frameshift activity presented profound defects in viral replication. In contrast, viruses with stem-loop structures associated with 16 to 42% reductions in frameshift efficiency displayed no significant viral replication deficit.
journal_name
J Viroljournal_title
Journal of virologyauthors
Telenti A,Martinez R,Munoz M,Bleiber G,Greub G,Sanglard D,Peters Sdoi
10.1128/jvi.76.15.7868-7873.2002subject
Has Abstractpub_date
2002-08-01 00:00:00pages
7868-73issue
15eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
杂志文章abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) K1 gene encodes a polypeptide bearing an immunoreceptor tyrosine-based activation motif (ITAM) that is constitutively active for ITAM-based signal transduction. Although ectopic overexpression of K1 in cultured fibroblasts can lead to growth transformation, in vivo th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5891-5898.2001
更新日期:2001-07-01 00:00:00
abstract::Using synthetic peptides, we characterized the B-lymphocyte (antibody) and T-lymphocyte (proliferation) responses to an immunodominant epitope of human immunodeficiency virus type 1 (HIV-1) located near the amino-terminal end of the transmembrane glycoprotein (env amino acids 598 to 609). Both immunoglobulin M (IgM) a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2531-2536.1988
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doi:10.1128/JVI.66.2.1040-1049.1992
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journal_title:Journal of virology
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doi:10.1128/JVI.01078-07
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journal_title:Journal of virology
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doi:10.1128/JVI.62.2.552-557.1988
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.2754-2762.1992
更新日期:1992-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2020-02-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.415-423.1991
更新日期:1991-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.14.2.349-365.1974
更新日期:1974-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.906-913.1992
更新日期:1992-02-01 00:00:00
abstract:UNLABELLED:Bluetongue is one of the major infectious diseases of ruminants and is caused by bluetongue virus (BTV), an arbovirus existing in nature in at least 26 distinct serotypes. Here, we describe the development of a vaccine platform for BTV. The advent of synthetic biology approaches and the development of revers...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02183-14
更新日期:2014-11-01 00:00:00
abstract::Rio Bravo virus (RBV) is a member of the family Flaviviridae, genus Flavivirus. It belongs to a group of viruses in the genus with no known vector. In this report, we analyze the complete genome of the prototype RBV, strain M64. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00331-12
更新日期:2012-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1977-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3198-3206.1996
更新日期:1996-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.328-334.1986
更新日期:1986-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.974-981.1975
更新日期:1975-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8182-8186.1996
更新日期:1996-11-01 00:00:00
abstract::Infectious pancreatic necrosis virus (IPNV) is a bisegmented, double-stranded RNA (dsRNA) virus of the Birnaviridae family that causes widespread disease in salmonids. Its two genomic segments are encapsulated together with the viral RNA-dependent RNA polymerase, VP1, and the assumed internal protein, VP3, in a single...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02831-06
更新日期:2007-06-01 00:00:00
abstract::A phylogenetic analysis of 52 published and 37 new nucleoprotein (NP) gene sequences addressed the evolution and origin of human and swine influenza A viruses. H1N1 human and classical swine viruses (i.e., those related to Swine/Iowa/15/30) share a single common ancestor, which was estimated to have occurred in 1912 t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3704-3714.1991
更新日期:1991-07-01 00:00:00