Abstract:
:We examined cellular components which associate with p40tax, the viral transactivation molecule of human T-cell leukemia virus type I. Such molecules were searched by immunoprecipitation with polyclonal and monoclonal antibodies specific for p40tax. Two cellular proteins with molecular masses of 95 kDa (p95) and 60 kDa (p60) were specifically coprecipitated with p40tax from extracts of all p40tax-producing cell lines but not from p40tax-negative cell lines. The p60 component was also shown to associate with p40tax in vitro, by using radiolabel-chase experiments. Rabbit antisera specific for p60 and p95 were prepared by immunization with electrophoretically purified molecules. While anti-p95 antiserum coprecipitated p40tax, no p40tax could be identified in immunoprecipitates by using a polyclonal anti-p60 antiserum. The partial amino acid sequence of p60 demonstrated that p60 is identical to the human 60-kDa heat shock protein (a member of the chaperonin family of proteins). Although the biological significance of the complex formation of p40tax with p95 and p60 has yet to be determined, it may be that the complex formation is one of the mechanisms by which the biological activity of p40tax can be regulated.
journal_name
J Viroljournal_title
Journal of virologyauthors
Nagata K,Ide Y,Takagi T,Ohtani K,Aoshima M,Tozawa H,Nakamura M,Sugamura Kdoi
10.1128/JVI.66.2.1040-1049.1992subject
Has Abstractpub_date
1992-02-01 00:00:00pages
1040-9issue
2eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::The majority of HIV-1 infections around the world result from non-B clade HIV-1 strains. The CRF01_AE (AE) strain is seen principally in Southeast Asia. AE protease differs by approximately 10% in amino acid sequence from clade B protease and carries several naturally occurring polymorphisms that are associated with d...
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pub_type: 杂志文章
doi:10.1128/JVI.44.2.603-609.1982
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1128/JVI.19.1.61-81.1976
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pub_type: 杂志文章
doi:10.1128/JVI.63.12.5354-5363.1989
更新日期:1989-12-01 00:00:00
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更新日期:1975-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
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