Abstract:
:Beta interferon (IFN-beta) expression is triggered by double-stranded RNA, a common intermediate in the replication of many viruses including hepatitis C virus (HCV). The recent development of cell culture-grown HCV allowed us to analyze the IFN signaling pathway following virus infection. In this study, we have examined the IFN-beta signaling pathway following infection of immortalized human hepatocytes (IHH) with HCV genotype 1a (clone H77) or 2a (clone JFH1). We observed that IHH possesses a functional Toll-like receptor 3 pathway. HCV infection in IHH enhanced IFN-beta and IFN-stimulated gene 56 (ISG56) promoter activities; however, poly(I-C)-induced IFN-beta and ISG56 expression levels were modestly inhibited upon HCV infection. IHH infected with HCV (genotype 1a or 2a) exhibited various levels of translocation of IRF-3 into the nucleus. The upregulation of endogenous IFN-beta and 2',5'-oligoadenylate synthetase 1 mRNA expression was also observed in HCV-infected IHH. Subsequent studies suggested that HCV infection in IHH enhanced STAT1 and ISG56 protein expression. A functional antiviral response of HCV-infected IHH was observed by the growth-inhibitory role in vesicular stomatitis virus. Together, our results suggested that HCV infection in IHH induces the IFN signaling pathway, which corroborates observations from natural HCV infection in humans.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kanda T,Steele R,Ray R,Ray RBdoi
10.1128/JVI.01695-07subject
Has Abstractpub_date
2007-11-01 00:00:00pages
12375-81issue
22eissn
0022-538Xissn
1098-5514pii
JVI.01695-07journal_volume
81pub_type
杂志文章abstract::BALB/c mice infected on the scarified cornea with herpes simplex virus type 1 strain 35 [HSV-1(35)] rarely developed ocular disease even at challenge doses as high as 10(7) PFU per eye. In contrast, HSV-1(RE) consistently induced stromal keratitis at an inoculum of 2 x 10(4) PFU. The goal of this study was to determin...
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pub_type: 杂志文章
doi:10.1128/JVI.64.5.2187-2192.1990
更新日期:1990-05-01 00:00:00
abstract::The Tax protein of human T-cell leukemia virus type 1 (HTLV-1) is a potent activator of viral transcription. Tax also activates the expression of specific cellular genes involved in the control of T-lymphocyte growth via effects on cellular transcription factors, including members of the NF-kappaB/cRel family. Immunoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3484-3497.1997
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abstract::Intrarectal simian immunodeficiency virus (SIV) infection in rhesus macaques is a model for sexual transmission of primate retroviruses. Phylogenetic studies on envelope gene sequences that were present in blood following intrarectal SIV inoculation provided evidence for selective amplification of a subset of viruses ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6876-6883.1996
更新日期:1996-10-01 00:00:00
abstract::The papillomavirus capsid mediates binding to the cell surface and passage of the virion to the perinuclear region during infection. To better understand how the virus traffics across the cell, we sought to identify cellular proteins that bind to the minor capsid protein L2. We have identified syntaxin 18 as a protein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.6723-6731.2005
更新日期:2005-06-01 00:00:00
abstract::Reverse genetic analyses of negative-strand RNA (NSR) viruses have provided enormous advances in our understanding of animal viruses over the past 20 years, but technical difficulties have hampered application to plant NSR viruses. To develop a reverse genetic approach for analysis of plant NSR viruses, we have engine...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
abstract::Recent studies of varicella-zoster virus (VZV) DNA sequence variation, involving large numbers of globally distributed clinical isolates, suggest that this virus has diverged into at least three distinct genotypes designated European (E), Japanese (J), and mosaic (M). In the present study, we determined and analyzed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00835-06
更新日期:2006-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.380-382.1981
更新日期:1981-04-01 00:00:00
abstract::Molecular clones of the subgroup A feline leukemia virus FeLV-A/Glasgow-1 have been obtained. Nucleotide sequence analysis of the 3' end of the proviral genome and comparison with the published sequence of FeLV-B/Gardner-Arnstein showed that the most extensive differences are located within the 5' domain of the env ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.825-834.1986
更新日期:1986-06-01 00:00:00
abstract::Amphiphilic fatty acid derivatives having a 14- to 20-carbon cis-unsaturated alkyl chain were found to be potent inhibitors of the entry process of the lipid-containing bacteriophage PR4. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.413-416.1979
更新日期:1979-01-01 00:00:00
abstract::Human papillomavirus type 16 (HPV16) is the primary etiologic agent for cervical cancer. The infectious entry of HPV16 into cells occurs via a so-far poorly characterized clathrin- and caveolin-independent endocytic pathway, which involves tetraspanin proteins and actin. In this study, we investigated the specific rol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02906-12
更新日期:2013-03-01 00:00:00
abstract::An R638A mutation of the polymerase acidic protein (PA) subunit of the RNA polymerase of influenza A/WSN/33 virus results in severe attenuation of viral growth in cell culture by promoting the synthesis of defective interfering RNAs. We propose that R638A is an "elongation" mutant that destabilizes PA-RNA template int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.5017-5020.2003
更新日期:2003-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.6953-6959.1992
更新日期:1992-12-01 00:00:00
abstract::The Zaire ebolavirus (EBOV) glycoprotein (GP) is cleaved into two subunits (GP1 and GP2) that are both required for virus attachment and entry into cells. Sequence changes in the GP have been proposed to increase pathogenesis and to alter virus growth properties. Mutations in GP acquired during EBOV tissue culture pas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00820-18
更新日期:2018-09-12 00:00:00
abstract::The cellular transcription factors Sp1 and NF-kappaB were upregulated shortly after the binding of purified live or UV-inactivated human cytomegalovirus (HCMV) to the cell surface. The rapid time frame of transcription factor induction is similar to that seen in other systems in which cellular factors are induced foll...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5051-5059.1997
更新日期:1997-07-01 00:00:00
abstract::Influenza A viruses (IAV) are lytic viruses that have recently been found to activate necroptosis in many of the cell types they infect. Necroptotic cell death is potently immunogenic and limits IAV spread by directly eliminating infected cells and by mobilizing both innate and adaptive immune responses. The benefits ...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01101-19
更新日期:2020-04-16 00:00:00
abstract:UNLABELLED:Influenza virus transcription requires functional coupling with cellular transcription for the cap-snatching process. Despite this fact, RNA polymerase II (RNAP II) is degraded during infection in a process triggered by the viral polymerase. Reassortant viruses from the A/PR/8/34 (PR8) strain that induce (hv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02263-13
更新日期:2014-03-01 00:00:00
abstract::Rigorous investigation of many functions encoded by cytomegaloviruses (CMVs) requires analysis in the context of virus-host interactions. To facilitate the construction of rhesus CMV (RhCMV) mutants for in vivo studies, a bacterial artificial chromosome (BAC) containing an enhanced green fluorescent protein (EGFP) cas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.9.5073-5083.2003
更新日期:2003-05-01 00:00:00
abstract::While RNA silencing is a potent antiviral defense in plants, well-adapted plant viruses are known to encode suppressors of RNA silencing (VSR) that can neutralize the effectiveness of RNA silencing. As a result, most plant genes involved in antiviral silencing were identified by using debilitated viruses lacking silen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00497-12
更新日期:2012-06-01 00:00:00
abstract::We have studied the unintegrated infectious DNA of Harvey sarcoma virus (Ha-SV) and Moloney leukemia virus (Mo-MuLV). The source of infectious viral DNA was the Hirt supernatant fraction from cells acutely infected with Ha-SV and Mo-MuLV. To obtain a direct quantitative assay for infectious viral DNA, recipient mouse ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.291-298.1978
更新日期:1978-05-01 00:00:00
abstract::Extracts from poliovirus-infected HeLa cells are unable to translate vesicular stomatitis virus or cellular mRNAs in vitro, probably reflecting the poliovirus-induced inhibition of host cell protein synthesis which occurs in vivo. Crude initiation factors from uninfected HeLa cells are able to restore translation of v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.200-207.1982
更新日期:1982-04-01 00:00:00
abstract::The spindles of Anomala cuprea entomopoxvirus (AncuEPV), which are composed of glycoprotein fusolin, are known to enhance the peroral infectivity of AncuEPV itself and of nucleopolyhedroviruses. This has been demonstrated to involve the disruption of intestinal peritrophic membrane (PM), composed of chitin matrix, gly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01605-08
更新日期:2008-12-01 00:00:00
abstract::Numerous RNA viruses generate subgenomic mRNAs (sgRNAs) for expression of their 3'-proximal genes. A major step in control of viral gene expression is the regulation of sgRNA synthesis by specific promoter elements. We used barley yellow dwarf virus (BYDV) as a model system to study transcriptional control in a virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.5988-5996.2000
更新日期:2000-07-01 00:00:00
abstract::A mutation that disrupts the interaction between the NS2 protein of minute virus of mice and the nuclear export factor Crm1 results in a block to egress of mutant-generated full virions from the nucleus of infected murine cells. These mutants produce wild-type levels of monomer and dimer replicative DNA forms but are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3257-3266.2002
更新日期:2002-04-01 00:00:00
abstract::Harvard Medical School convened a meeting of biomedical and clinical experts on 5 March 2015 on the topic of "Rethinking the Response to Emerging Microbes: Vaccines and Therapeutics in the Ebola Era," with the goals of discussing the lessons from the recent Ebola outbreak and using those lessons as a case study to aid...
journal_title:Journal of virology
pub_type: 共识发展会议,杂志文章
doi:10.1128/JVI.01251-15
更新日期:2015-08-01 00:00:00
abstract::We located a region of interstrain size variability in a short segment in an area at the right-hand end of the long unique sequence of the varicella-zoster viral genome. Varicella-zoster virus strains isolated in a district of Japan were classified into three groups on the basis of the size of this segment. Sequence c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2916-2921.1988
更新日期:1988-08-01 00:00:00
abstract::Recent in vitro proteomics screens revealed that many host proteins could interact with the replication proteins of Tomato bushy stunt virus (TBSV), which is a small, plus-stranded RNA virus (Z. Li, D. Barajas, T. Panavas, D. A. Herbst, and P. D. Nagy, J. Virol. 82:6911-6926, 2008). To further our understanding of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00789-09
更新日期:2009-11-01 00:00:00
abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 57 (ORF57)-encoded protein (Mta) is a multifunctional regulator of viral gene expression. ORF57 is essential for viral replication, so elucidation of its molecular mechanisms is important for understanding KSHV infection. ORF57 has been implicated i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03459-12
更新日期:2013-04-01 00:00:00
abstract:UNLABELLED:Human cytomegalovirus (hCMV) infection is characterized by a vast expansion of resting effector-type virus-specific T cells in the circulation. In mice, interleukin-7 receptor α (IL-7Rα)-expressing cells contain the precursors for long-lived antigen-experienced CD8(+) T cells, but it is unclear if similar me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02003-14
更新日期:2015-01-01 00:00:00
abstract::Most eukaryotic initiation factors (eIFs) are required for internal translation initiation at the internal ribosome entry site (IRES) of picornaviruses. eIF4B is incorporated into ribosomal 48S initiation complexes with the IRES RNA of foot-and-mouth disease virus (FMDV). In contrast to the weak interaction of eIF4B w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7505-7514.1999
更新日期:1999-09-01 00:00:00
abstract::In an effort to develop a useful AIDS vaccine or vaccine component, we have generated a combinatorial library of chimeric viruses in which the sequence IGPGRAFYTTKN from the V3 loop of the MN strain of human immunodeficiency virus type 1 (HIV-1) is displayed in many conformations on the surface of human rhinovirus 14 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.651-659.1998
更新日期:1998-01-01 00:00:00