Abstract:
:Extracts from poliovirus-infected HeLa cells are unable to translate vesicular stomatitis virus or cellular mRNAs in vitro, probably reflecting the poliovirus-induced inhibition of host cell protein synthesis which occurs in vivo. Crude initiation factors from uninfected HeLa cells are able to restore translation of vesicular stomatitis virus mRNA in infected cell lysates. This restoring activity separates into the 0 to 40% ammonium sulfate fractional precipitate of ribosomal salt wash. Restoring activity is completely lacking in the analogous fractions prepared from poliovirus-infected cells. The 0 to 40% ammonium sulfate precipitates from both uninfected and infected cells contain eucaryotic initiation factor 3 (eIF-3), eIf-4B, and the cap-binding protein (CBP), which is detected by means of a cross-linking assay, as well as other proteins. The association of eIF-3 and cap binding protein was examined. The 0 to 40% ammonium sulfate precipitate of ribosomal salt wash from uninfected and infected cells was sedimented in sucrose gradients. Each fraction was examined for the presence of eIF-3 antigens by an antibody blot technique and for the presence of the CBP by cross-linking to cap-labeled mRNAs. From uninfected cells, a major proportion of the CBP cosedimented with eIF-3; however, none of the CBP from infected cells sedimented with eIF-3. The results suggest that the association of the CBP with eIF-3 into a functional complex may have been disrupted during the course of poliovirus infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hansen J,Etchison D,Hershey JW,Ehrenfeld Edoi
10.1128/JVI.42.1.200-207.1982subject
Has Abstractpub_date
1982-04-01 00:00:00pages
200-7issue
1eissn
0022-538Xissn
1098-5514journal_volume
42pub_type
杂志文章abstract::Equine infectious anemia virus (EIAV) is a lentivirus with in vivo cell tropism primarily for tissue macrophages; however, in vitro the virus can be adapted to fibroblasts and other cell types. Tropism adaptation is associated with both envelope and long terminal repeat (LTR) changes, and findings strongly suggest tha...
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.52.3.913-918.1984
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.1.28-32.1970
更新日期:1970-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1678-1681.1987
更新日期:1987-05-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.66.9.5553-5560.1992
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00