Abstract:
:The papillomavirus E2 gene product plays a pivotal role in viral replication. E2 has multiple functions, including (i) transcriptional activation and repression of viral promoters and (ii) the enhancement of viral DNA replication. It was previously reported that E2 suppressed the growth of papillomavirus-positive cervical carcinoma cell lines. In the present study, we investigated the mechanisms of E2 growth inhibition. We found that the transcriptional activation function of E2 is required for inhibition of the growth of HeLa cells as well as for transcriptional repression of the viral E6/E7 promoter. It had been previously postulated that transcriptional repression of the E6/E7 promoter results from E2 binding its cognate sites proximal to the E6/E7 promoter and displacing other cellular transcriptional factors. In this study, we report a requirement for the transcription activation function for the binding of E2 to transcriptionally active templates.
journal_name
J Viroljournal_title
Journal of virologyauthors
Nishimura A,Ono T,Ishimoto A,Dowhanick JJ,Frizzell MA,Howley PM,Sakai Hdoi
10.1128/jvi.74.8.3752-3760.2000subject
Has Abstractpub_date
2000-04-01 00:00:00pages
3752-60issue
8eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
杂志文章abstract::Viruses efficiently block the host antiviral response in order to replicate and spread before host intervention. The mechanism initiating antiviral immunity during stealth viral replication is unknown, but recent data demonstrate that defective viral genomes generated at peak virus replication are critical for this pr...
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journal_title:Journal of virology
pub_type: 杂志文章
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abstract::The amino-terminal region of the Vif molecule in human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus (SIV) contains a conserved SLV/Ix4Yx9Y motif that was first described in 1992, but the importance of this motif for Vif function has not yet been examined. Our characterization of the ...
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doi:10.1128/JVI.00653-09
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abstract::We have used immunofluorescence in parallel with transmission and scanning electron microscopy to characterize the unusual cytoplasmic and nucleolar accumulation of Simian virus 40 (SV40) virion protein (C antigen) at restrictive temperatures (39 to 41 C) in monkey cells infected with a temperature-sensitive mutant of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.6.1530-1546.1974
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abstract::Klebsiella pneumoniae is a member of the family Enterobacteriaceae, opportunistic pathogens that are among the eight most prevalent infectious agents in hospitals. The emergence of multidrug-resistant strains of K. pneumoniae has became a public health problem globally. To develop an effective antimicrobial agent, we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02435-12
更新日期:2012-12-01 00:00:00
abstract::In this report, we describe some phenotypic properties of a temperature-sensitive mutant of herpes simplex type 1 (HSV-1) and present data concerning the physical location and nucleotide sequence of the genomic region harboring the mutation. The effect of shifts from the permissive to the nonpermissive temperature on ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2169-2179.1989
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abstract::African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal viral disease of domestic pigs that has significant economic consequences for the swine industry. The control of African swine fever (ASF) has been hampered by the unavailability of vaccines. Successful experimental vaccines hav...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.180-185.1983
更新日期:1983-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.13.2.411-418.1974
更新日期:1974-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.186-193.1985
更新日期:1985-04-01 00:00:00
abstract::Infectious bursal disease virus (IBDV) is a double-stranded RNA (dsRNA) virus of the Birnaviridae family. Its two genome segments are encapsidated together with multiple copies of the viral RNA-dependent RNA polymerase, VP1, in a single-shell capsid that is composed of VP2 and VP3. In this study we identified the doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11301-11311.2002
更新日期:2002-11-01 00:00:00
abstract::We have characterized by T1 fingerprint analysis several defective interfering (DI) double-stranded RNAs of the simple yeast virus ScV. A common sequence of about 0.5 to 0.6 kilobase pairs, including both 3' termini of the parental RNA, was present in each DI RNA. Several DI RNAs had novel T1 oligonucleotides not pres...
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pub_type: 杂志文章
doi:10.1128/JVI.32.2.692-696.1979
更新日期:1979-11-01 00:00:00
abstract::The parameters governing the activity of the cloned T4 gene 23, which codes for the major T4 head protein, were analyzed. Suppressor-negative bacteria carrying wild-type T4 gene 23 cloned into plasmid pCR1 or pBR322 were infected with T4 gene 23 amber phage also carrying mutations in the following genes: (i) denA and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.46-59.1981
更新日期:1981-07-01 00:00:00
abstract::Since 2005, a large poliomyelitis outbreak associated with type 2 circulating vaccine-derived poliovirus (cVDPV2) has occurred in northern Nigeria, where immunization coverage with trivalent oral poliovirus vaccine (tOPV) has been low. Phylogenetic analysis of P1/capsid region sequences of isolates from each of the 40...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02954-12
更新日期:2013-05-01 00:00:00
abstract::Integrated retroviruses or retroviral vectors may be transcriptionally inactive although their promoter-enhancer regions are able to direct transcription in the host cell. We have used single-gene retroviral vectors with a long terminal repeat-directed neo marker gene to determine if the level of transcription relates...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5596-5601.1994
更新日期:1994-09-01 00:00:00
abstract::Polypeptide synthesis has been studied in cell-free systems prepared from vaccinia virus-infected and uninfected HeLa cells. Cytoplasmic extracts containing endogenous messenger ribonucleic acid were used. Amino acid incorporation into hot trichloroacetic acid-precipitable material was linear for 15 to 20 min at 37 C....
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pub_type: 杂志文章
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更新日期:1969-10-01 00:00:00
abstract::Two foot-and-mouth disease virus (FMDV) genome sequences have been determined for isolates collected from recent field outbreaks in North Africa (Egypt) and the Middle East (Palestinian Autonomous Territories). These data represent the first examples of complete genomic sequences for the FMDV SAT 2 topotype VII, which...
journal_title:Journal of virology
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更新日期:2012-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3103-3110.1993
更新日期:1993-06-01 00:00:00
abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) nsp1 protein has unique biological functions that have not been described in the viral proteins of any RNA viruses; expressed SARS-CoV nsp1 protein has been found to suppress host gene expression by promoting host mRNA degradation and inhibiting translation....
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
abstract:UNLABELLED:Susceptibility to alphavirus infection is age dependent, and host maturation is associated with decreased virus replication and less severe encephalitis. To identify factors associated with maturation-dependent restriction of virus replication, we studied AP-7 rat olfactory bulb neuronal cells, which can dif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02394-14
更新日期:2015-01-01 00:00:00
abstract::Expression of the selectable drug resistance gene in retroviral vectors used for gene therapy can lead to a decreased expression of the gene of interest and may induce a host immune response, resulting in a decreased efficiency of gene therapy. In this study, we demonstrate that high-frequency deletion of direct repea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8837-8842.1999
更新日期:1999-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.7217-7226.2005
更新日期:2005-06-01 00:00:00
abstract::The nuclear egress complex (NEC) is required for efficient translocation of newly synthesized herpesvirus nucleocapsids from the nucleus to the cytosol. It consists of the type II membrane protein pUL34 which interacts with pUL31 at the inner nuclear membrane (INM). To map regions within pUL34 required for nuclear mem...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00021-13
更新日期:2013-04-01 00:00:00
abstract:UNLABELLED:Little is known about the antiviral response in mollusks. As in other invertebrates, the interferon signaling pathways have not been identified, and in fact, there is a debate about whether invertebrates possess antiviral immunity similar to that of vertebrates. In marine bivalves, due to their filtering act...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00591-16
更新日期:2016-08-12 00:00:00
abstract::Previous reports (H. Delius and J. B. Clements, J. Gen. Virol. 33:125-134, 1976; G. S. Hayward, R. J. Jacob, S. C. Wadsworth, and B. Roizman, Proc. Natl. Acad. Sci. U.S.A. 72:4243-4247, 1975; B. Roizman, G. S. Hayward, R. Jacob, S. W. Wadsworth, and R. W. Honess, Excerpta Med. Int. Congr. Ser. 2:188-198, 1974) have sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.448-457.1979
更新日期:1979-02-01 00:00:00
abstract::Polyomavirus large T antigen has an N-terminal domain of approximately 260 amino acids which can immortalize primary cells but lacks sequences known to be required for DNA binding and replication. Treatment of full-length large T with either V8 protease or chymotrypsin yields an N-terminal fragment of 36 to 40 kDa and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.668-673.1994
更新日期:1994-02-01 00:00:00
abstract::As a subunit of both the P-L polymerase complex and the P-N assembly complex, the vesicular stomatitis virus (VSV) P protein plays a pivotal role in transcription and replication of the viral genome. Constitutive phosphorylation of this protein is currently thought to be essential for formation of the P-L complex. We ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4538-4548.1996
更新日期:1996-07-01 00:00:00