Abstract:
:All herpesviruses encode a homolog of glycoprotein M (gM), which appears to function in virion morphogenesis. Despite its conservation, gM is inessential for the lytic replication of alphaherpesviruses. In order to address the importance of gM in gammaherpesviruses, we disrupted it in the murine gammaherpesvirus 68 (MHV-68). The mutant virus completely failed to propagate in normally permissive fibroblasts. The defective genome was rescued by either homologous recombination to restore the wild-type gM in situ or the insertion of an ectopic, intergenic expression cassette encoding gM into the viral genome. Thus, gM was essential for the lytic replication of MHV-68.
journal_name
J Viroljournal_title
Journal of virologyauthors
May JS,Colaco S,Stevenson PGdoi
10.1128/JVI.79.6.3459-3467.2005subject
Has Abstractpub_date
2005-03-01 00:00:00pages
3459-67issue
6eissn
0022-538Xissn
1098-5514pii
79/6/3459journal_volume
79pub_type
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