Abstract:
:Polyoma virus particles were purified by equilibrium centrifugation in CsCl. Particles from three regions of the density gradient were examined for infectivity, for their ability to induce expanded pools of deoxyribonucleic acid (DNA) precursors, and for their ability to stimulate the synthesis of DNA. The most infectious population of particles, the virions, having a buoyant density of 1.33 g/ml, gave the greatest stimulation of the DNA-synthesizing apparatus of mouse embryo cells. Empty particles at density 1.29 g/ml had no DNA stimulatory activity. A population of particles of intermediate density, referred to as pseudovirions, was also much less active than virions in stimulating DNA synthesis, and the limited stimulatory activity of the latter fraction may be accounted for by its measured contamination with infective particles.
journal_name
J Viroljournal_title
Journal of virologyauthors
Skoog L,Nordenskjöld BA,Lindberg Udoi
10.1128/JVI.6.1.28-32.1970subject
Has Abstractpub_date
1970-07-01 00:00:00pages
28-32issue
1eissn
0022-538Xissn
1098-5514journal_volume
6pub_type
杂志文章abstract::Enteroviral persistence has been implicated in the pathogenesis of several chronic human diseases, including dilated cardiomyopathy, insulin-dependent diabetes mellitus, and chronic inflammatory myopathy. However, these viruses are considered highly cytolytic, and it is unclear what mechanisms might permit their long-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4430-4440.2002
更新日期:2002-05-01 00:00:00
abstract::Earlier studies have shown that herpes simplex viruses adsorb to but do not penetrate permissive baby hamster kidney clonal cell lines designated the BJ series and constitutively expressing the herpes simplex virus 1 (HSV-1) glycoprotein D (gD). To investigate the mechanism of the restriction, the following steps were...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.6070-6079.1990
更新日期:1990-12-01 00:00:00
abstract::The fusion (F) protein of simian virus 5 (strain W3A) induced extensive cell fusion in BHK cells when expressed alone, while that of strain WR did not. Mutational analysis demonstrated that the fusing activity can be transferred to the WR F protein by a proline residue at position 22 of subunit W3A F2. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9855-9858.1997
更新日期:1997-12-01 00:00:00
abstract:UNLABELLED:The HIV-1 envelope protein (Env) is heavily glycosylated, with approximately 50% of the Env molecular mass being contributed by N-glycans. HIV-1 Env N-glycans shield the protein backbone and have been shown to play key roles in determining Env structure, surface exposure, and, consequently, antigenicity, inf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02164-14
更新日期:2014-12-01 00:00:00
abstract::Retroviral Gag proteins have the ability to induce budding and particle release from the plasma membrane when expressed in the absence of all of the other virus-encoded components; however, the locations of the functional domains within the Gag protein that are important for this process are poorly understood. It was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5550-5561.1993
更新日期:1993-09-01 00:00:00
abstract::A temperature-sensitive mutant of adenovirus 31 was defective in formation of the DNA replication complex, suggesting the existence of a virus-coded protein necessary for the complex-formation. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.538-540.1974
更新日期:1974-02-01 00:00:00
abstract::All hepadnaviruses known so far have a very limited host range, restricted to their natural hosts and a few closely related species. This is thought to be due mainly to sequence divergence in the large envelope protein and species-specific differences in host components essential for virus propagation. Here we report ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.1964-1976.2003
更新日期:2003-02-01 00:00:00
abstract::IgG1 b12 is a broadly neutralizing antibody against human immunodeficiency virus type 1 (HIV-1). The epitope recognized by b12 overlaps the CD4 receptor-binding site (CD4bs) on gp120 and has been a target for vaccine design. Determination of the three-dimensional structure of immunoglobulin G1 (IgG1) b12 allowed model...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5863-5876.2003
更新日期:2003-05-01 00:00:00
abstract::Eight nonoverlapping regions of the hemagglutinin (HA) molecule of influenza virus A/PR/8/34 (PR8), which serve as recognition sites for class II-restricted T cells (TH) from BALB/c mice, have been identified in the form of 10- to 15-amino-acid-long synthetic peptides. These TH determinants are located between residue...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.364-372.1991
更新日期:1991-01-01 00:00:00
abstract::A lysate from hypotonically swollen polyoma-infected BALB/3T3 cells incorporated labeled deoxynucleotide triphosphates into both viral and cellular DNAs. The incorporation was stimulated by the presence of ATP, deoxynucleotide triphosphates, thiols, and magnesium ions. Strong inhibition of incorporation was observed w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.125-139.1974
更新日期:1974-01-01 00:00:00
abstract::Human cytomegalovirus (HCMV) exhibits a highly restricted host range. In this study, we sought to examine the relative significance of host and viral factors in activating early gene expression of the HCMV UL54 (DNA polymerase) promoter in murine cells. Appropriate activation of the UL54 promoter at early times is ess...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.26-35.2001
更新日期:2001-01-01 00:00:00
abstract::Tryptic peptide maps were prepared for four purified structural proteins derived from several murine leukemia viruses (MuLV's). Analyses of these peptide maps reveal that the p30 proteins of Rauscher, Moloney, and Gross MuLV's are very similar to each other, as are the p10's obtained from these three viruses. In contr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.5.1148-1157.1975
更新日期:1975-05-01 00:00:00
abstract::An immediate-early protein of murine cytomegalovirus (MCMV), pp89, elicits an immunodominant and protective major histocompatibility complex (MHC) class I Ld-restricted CD8+ T-lymphocyte response. Remarkably, presentation of the naturally processed peptide of pp89, the nonapeptide YPHFMPTNL, is abolished during permis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.289-297.1994
更新日期:1994-01-01 00:00:00
abstract::Poxviruses express proteins that limit host immune responses to infection. For example, the molluscum contagiosum virus MC160 protein inhibits tumor necrosis factor alpha (TNF-alpha)-induced NF-kappaB activation. This event correlates with MC160-induced IKK1 protein degradation, suggesting a mechanism for the above-me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02009-08
更新日期:2009-04-01 00:00:00
abstract::Despite being able to use the Bonzo coreceptor as efficiently as CCR5 in transfected cells, pediatric human immunodeficiency virus type 1 isolate P6 was unable to replicate in peripheral blood mononuclear cells (PBMC) lacking the CCR5 receptor. Furthermore, its replication in wild-type PBMC was completely inhibited by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3443-3448.1999
更新日期:1999-04-01 00:00:00
abstract::Bacteriophage T4 gene 32 encodes a DNA unwinding protein required for DNA replication, repair, and recombination. Gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.490-494.1976
更新日期:1976-08-01 00:00:00
abstract::The L1 and L2 capsid genes of human papillomavirus type 31 (HPV-31) are expressed upon keratinocyte differentiation from a promoter located in the E7 open reading frame (ORF) of the early region. Late transcripts must therefore pass through and ignore the early polyadenylation sequences to use the downstream late AAUA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.17.8147-8157.2001
更新日期:2001-09-01 00:00:00
abstract::The gene encoding the precursor polypeptide of the Marek's disease herpesvirus (MDHV) secretory glycoprotein gp57-65 (formerly identified as A antigen) has been sequenced. Previous results had localized the gene to a 4.6-kilobase (kb) segment of the BamHI B fragment in the unique long region of the MDHV genome. S1 nuc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2373-2379.1988
更新日期:1988-07-01 00:00:00
abstract::RNA packaging signals (psi) from the 5' ends of murine and avian retroviral genomes have previously been shown to direct encapsidation of heterologous mRNA into the retroviral virion. The avian 5' packaging region has now been further characterized, and we have defined a 270-nucleotide sequence, A psi, which is suffic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.178-188.1993
更新日期:1993-01-01 00:00:00
abstract::Previously we have described a stepwise, energy-dependent pathway for human immunodeficiency virus type 1 (HIV-1) capsid assembly in a cell-free system. In this pathway, Gag polypeptides utilize the cellular factor HP68 and assemble into immature capsids by way of assembly intermediates that have defined biochemical c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1645-1656.2004
更新日期:2004-02-01 00:00:00
abstract::The Epstein-Barr virus (EBV) major envelope glycoprotein gp340 is the subject of current efforts to develop an EBV subunit vaccine. The importance of gp340-specific humoral immunity has been highlighted by studies of natural infection in humans and gp340 immunization of experimental animals. The former studies have de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.1246-1251.1992
更新日期:1992-02-01 00:00:00
abstract::Proteolytic processing of the picornaviral polyprotein mediated by the differential action of virus-encoded proteinase(s) is pivotal to both RNA genome replication and capsid formation. Possibly to enlarge the array of viral proteins, picornaviral polyprotein processing results in intermediate and mature products whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8013-8020.1998
更新日期:1998-10-01 00:00:00
abstract::Alphaherpesviruses are parasites of the peripheral nervous system in their natural hosts. After the initial infection of peripheral tissues such as mucosal cells, these neurotropic viruses will invade the peripheral nervous system that innervates the site of infection via long-distance axonal transport of the viral ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.10875-10889.2005
更新日期:2005-09-01 00:00:00
abstract:UNLABELLED:Japanese encephalitis (JE) is an arthropod-borne disease associated with the majority of viral encephalitis cases in the Asia-Pacific region. The causative agent, Japanese encephalitis virus (JEV), has been phylogenetically divided into five genotypes. Recent surveillance data indicate that genotype I (GI) i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02050-14
更新日期:2014-10-01 00:00:00
abstract::CaM kinase-Gr is a multifunctional Ca2+/calmodulin-dependent protein kinase which is enriched in neurons and T lymphocytes. The kinase is absent from primary human B lymphocytes but is expressed in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell lines, suggesting that expression of the kinase can be upregul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1697-1705.1994
更新日期:1994-03-01 00:00:00
abstract::DNAs from four wild chimpanzees (Pan troglodytes schweinfurthi) from eastern Africa were screened for 14 DNA viruses and retroviruses. Between two and three viruses were found in each animal. An entire hepatitis B virus (HBV) genome was amplified and sequenced from samples taken from one animal. This indicates that HB...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.11155-11158.2002
更新日期:2002-11-01 00:00:00
abstract::Hepatitis C virus (HCV) core protein is suggested to localize to the endoplasmic reticulum (ER) through a C-terminal hydrophobic region that acts as a membrane anchor for core protein and as a signal sequence for E1 protein. The signal sequence of core protein is further processed by signal peptide peptidase (SPP). We...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6370-6380.2004
更新日期:2004-06-01 00:00:00
abstract::The release of the ribonucleic acid (RNA)-containing phage MS2 from Escherichia coli is accompanied by cellular lysis at 37 C, whereas at 30 C phage are released from intact cells. Chloramphenicol or rifampin prevents the release of progeny phage particles at both temperatures. Neither drug causes an immediate cessati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.3.257-264.1971
更新日期:1971-09-01 00:00:00
abstract::Immune responses to vector-corrected cells have limited the application of gene therapy for treatment of chronic disorders such as inherited deficiency states. We have found that recombinant adeno-associated virus (AAV) efficiently transduces muscle fibers in vivo without activation of cellular and humoral immunity to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4212-4223.1998
更新日期:1998-05-01 00:00:00
abstract::Major neutralization antigenic sites have been previously mapped by us on VP1, the largest capsid protein of poliovirus type 1. Here we report the first identification of the primary sequence of a neutralization antigenic site on capsid protein VP2. Inspection of the amino acid sequence of VP2 led to the selection and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.719-721.1984
更新日期:1984-11-01 00:00:00