Abstract:
:Previously we have described a stepwise, energy-dependent pathway for human immunodeficiency virus type 1 (HIV-1) capsid assembly in a cell-free system. In this pathway, Gag polypeptides utilize the cellular factor HP68 and assemble into immature capsids by way of assembly intermediates that have defined biochemical characteristics. Here we address whether this pathway is universally conserved among primate lentiviruses and can be observed in mammalian cells. We demonstrate that HIV-2 Gag associates with human HP68 in a cell-free system and that Gag proteins of HIV-2, simian immunodeficiency virus SIVmac239, and SIVagm associate with endogenous HP68 in primate cells, as is seen for HIV-1. Analysis of primate cells expressing lentivirus Gag proteins revealed Gag-containing complexes with the same sedimentation values as seen for previously described HIV-1 assembly intermediates in the cell-free system (10S, 80-150S, and 500S). These complexes fit criteria for assembly intermediates as judged by energy sensitivity, pattern of HP68 association, and the failure of specific complexes to be formed by assembly-incompetent Gag mutants. We also demonstrate that virus-like particles released from cells do not appear to contain HP68, suggesting that HP68 is released from Gag upon completion of capsid assembly in cells, as was observed previously in the cell-free system. Together these findings support a model in which all primate lentivirus capsids assemble by a conserved pathway of HP68-containing, energy-dependent assembly intermediates that have specific biochemical features.
journal_name
J Viroljournal_title
Journal of virologyauthors
Dooher JE,Lingappa JRdoi
10.1128/jvi.78.4.1645-1656.2004subject
Has Abstractpub_date
2004-02-01 00:00:00pages
1645-56issue
4eissn
0022-538Xissn
1098-5514journal_volume
78pub_type
杂志文章abstract::Infection with human T-cell leukemia virus type I (HTLV-I) is associated with adult T-cell lymphoma/leukemia. This disease occurs in only a small minority of people infected with HTLV-I and manifests itself many years after infection. Therefore, it appears that a fine balance exists between HTLV-I and the host T-cell ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5522-5528.1993
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abstract::Kaposi's sarcoma-associated herpesvirus (KSHV/human herpesvirus 8 [HHV-8]) is a gamma-2-herpesvirus responsible for Kaposi's sarcoma as well as primary effusion lymphoma (PEL). KSHV is a lymphotropic virus that has pirated many mammalian genes involved in inflammation, cell cycle control, and angiogenesis. Among these...
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pub_type: 杂志文章
doi:10.1128/jvi.77.1.57-67.2003
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doi:10.1128/JVI.62.10.3718-3728.1988
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journal_title:Journal of virology
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doi:10.1128/JVI.66.6.3330-3338.1992
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doi:10.1128/JVI.50.1.22-29.1984
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doi:10.1128/JVI.13.3.706-711.1974
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/JVI.33.2.866-876.1980
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02589-13
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.02080-07
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pub_type: 杂志文章
doi:10.1128/JVI.68.5.3397-3400.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7505-7514.1999
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pub_type: 杂志文章
doi:10.1128/JVI.73.9.7399-7409.1999
更新日期:1999-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.536-541.1986
更新日期:1986-05-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.43.2.503-510.1982
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doi:10.1128/JVI.4.3.244-251.1969
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3740-3748.1992
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2922-2927.1993
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1987-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.389-392.1981
更新日期:1981-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1991-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7181-7189.1993
更新日期:1993-12-01 00:00:00