Abstract:
:A versatile green fluorescent protein (GFP) expression cassette containing the replication origins of the monopartite begomovirus Tomato yellow leaf curl Sardinia virus (TYLCSV) is described. Transgenic Nicotiana benthamiana plants containing one copy of the cassette stably integrated into their genome were superinfected with TYLCSV, which mobilized and replicated the cassette as an episomal replicon. The expression of the reporter gene (the GFP gene) was thereby modified. Whereas GFP fluorescence was dimmed in the intercostal areas, an increase of green fluorescence in veins of all leaves placed above the inoculation site, as well as in transport tissues of roots and stems, was observed. The release of episomal trans replicons from the transgene and the increase in GFP expression were dependent on the cognate geminiviral replication-associated protein (Rep) and required interaction between Rep and the intergenic region of TYLCSV. This expression system is able to monitor the replication status of TYLCSV in plants, as induction of GFP expression is only produced in those tissues where Rep is present. To further confirm this notion, the expression of a host factor required for geminivirus replication, the proliferating cellular nuclear antigen (PCNA) was transiently silenced. Inhibition of PCNA prevented GFP induction in veins and reduced viral DNA. We propose that these plants could be widely used to easily identify host factors required for geminivirus replication by virus-induced gene silencing.
journal_name
J Viroljournal_title
Journal of virologyauthors
Morilla G,Castillo AG,Preiss W,Jeske H,Bejarano ERdoi
10.1128/JVI.80.7.3624-3633.2006subject
Has Abstractpub_date
2006-04-01 00:00:00pages
3624-33issue
7eissn
0022-538Xissn
1098-5514pii
80/7/3624journal_volume
80pub_type
杂志文章abstract:UNLABELLED:Hepatitis C virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma in humans. We showed previously that HCV induces autophagy for viral persistence by preventing the innate immune response. Knockdown of autophagy reduces extracellular HCV release, although the precis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02383-15
更新日期:2015-11-18 00:00:00
abstract::The cocultivation of a lung cell line from the Southeast Asian mouse Mus cervicolor with cells from heterologous species has resulted in the isolation of two new distinct type C viruses. Both viruses are endogenous to M. cervicolor and are present in multiple copies in the cellular DNA of these mice. One of the viruse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.849-862.1977
更新日期:1977-03-01 00:00:00
abstract::Human herpesvirus 8 (HHV8) open reading frame (ORF) 21 is predicted to encode a protein similar to the thymidine kinase (TK) enzyme of other herpesviruses. Expressed in mammalian cells, ORF 21 was found to have low TK activity, based on poor growth in media containing hypoxanthine-aminopterin-thymidine (HAT) and low i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.684-692.2000
更新日期:2000-01-01 00:00:00
abstract::Expression of the human ADA cDNA encoded by the Moloney murine leukemia virus spliced RNA form is enhanced by intron-contained sequences. The presence of sequences corresponding to the viral gag gene in a Moloney murine leukemia virus-based vector results in the generation of 10- to 40-fold higher titers of virus. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1647-1650.1987
更新日期:1987-05-01 00:00:00
abstract::We analyzed capsomeres of human papillomavirus type 16 (HPV16) consisting of the L1 major structural protein for their ability to trigger a cytotoxic T-cell (CTL) response. To this end, we immunized C57BL/6 mice and used the L1(165-173) peptide for ex vivo restimulation of splenocytes prior to analysis ((51)Cr release...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4635-4645.2003
更新日期:2003-04-01 00:00:00
abstract::It is shown that gene 5 protein of bacteriophage M13 is one of the major proteins synthesized in vitro under the direction of M13 replicative-form DNA. By means of DNA-cellulose chromatography, this protein has been purified to homogeneity and its biological characteristics have been compared with those of its native ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1466-1472.1973
更新日期:1973-12-01 00:00:00
abstract::The retroviral Gag precursor plays an important role in the assembly of virion particles. The capsid (CA) protein of the Gag molecule makes a major contribution to this process. In the crystal structure of the free CA protein of the human immunodeficiency virus type 1 (HIV-1), 11 residues of the C terminus were found ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9675-9688.2004
更新日期:2004-09-01 00:00:00
abstract::Arenaviruses are rodent-borne viruses, with five members of the family capable of causing severe hemorrhagic fevers if transmitted to humans. To date, two distinct cellular receptors have been identified that are used by different pathogenic viruses, alpha-dystroglycan by Lassa fever virus and transferrin receptor 1 (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01397-07
更新日期:2008-01-01 00:00:00
abstract::We have examined the pathway of rhinovirus interaction with soluble intercellular adhesion molecule 1 (sICAM-1). Binding of sICAM-1 to rhinovirus serotypes 3 and 14 gives particles with sedimentation coefficients from 145 to 120S, depending on the amount of sICAM-1 bound. The formation of 120S particles is faster and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5882-5889.1994
更新日期:1994-09-01 00:00:00
abstract::Persistent human immunodeficiency virus (HIV) infection of human monocytes and macrophages increases I kappa B alpha degradation, resulting in the activation of NF-kappa B, a key transcription factor in the regulation of the HIV long terminal repeat. The signal transduction pathways leading to NF-kappa B activation in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2332-2338.1996
更新日期:1996-04-01 00:00:00
abstract::In a previous study (S. Zheng, G. N. Woode, D. R. Melendy, and R. F. Ramig, J. Clin. Microbiol. 27:1939-1945, 1989), it was predicted that the VP7 serotype 6 bovine rotavirus strains NCDV and B641 do not share antigenically similar VP4s. In this study, gene 4 and the VP7 gene of B641 were sequenced, and the amino acid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5535-5538.1991
更新日期:1991-10-01 00:00:00
abstract::Injection of conventional or axenic weanling mice with potent sheep or goat antibody to mouse interferon alpha/beta resulted in a decrease in the basal level of 2-5A synthetase in resting peritoneal macrophages and rendered these cells permissive for vesicular stomatitis virus. There was a good inverse correlation bet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.1.221-227.1985
更新日期:1985-01-01 00:00:00
abstract::Currently there is limited information about the quality of immune responses elicited by candidate human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env)-based immunogens in primates. Here we describe a comprehensive analysis of neutralizing antibody and T-cell responses obtained in cynomolgus macaque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01102-08
更新日期:2009-01-01 00:00:00
abstract::The conversion of simian virus 40 (SV40) component II deoxyribonucleic acid to component I has been used to assay polynucleotide ligase in extracts of tissue culture cells. All cell types examined, including chicken, hamster, mouse, monkey, and human cells, contained adenosine triphosphate-dependent ligase. After infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.5.719-726.1969
更新日期:1969-11-01 00:00:00
abstract::Retroviruses are believed to induce tumors by acting as insertional mutagens that activate expression of cellular protooncogenes. Indeed, almost 90% of mouse mammary tumor virus (MMTV)-induced mammary tumors in C3H/He mice show upregulation of Int protooncogenes. We have analyzed three different MMTV variants [MMTV(C3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.8876-8883.2000
更新日期:2000-10-01 00:00:00
abstract::We have used linker scanning and site-directed mutagenesis in an attempt to distinguish among the known functions of the duck hepatitis B virus large envelope protein, p36. We found that linker-encoded amino acid substitutions in at least one region of the pre-S envelope protein p36 produced defects in both the produc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4565-4571.1994
更新日期:1994-07-01 00:00:00
abstract::Theiler's virus-induced demyelinating disease results from a chronic infection in the white matter of the central nervous system and provides an excellent model for human multiple sclerosis. Like multiple sclerosis, there are genetic risk factors in disease development, including genes associated with the major histoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.686-690.1990
更新日期:1990-02-01 00:00:00
abstract::The recent finding that the E1 glycoproteins of murine coronaviruses contain only O-linked oligosaccharides suggested that this unusual modification might be a distinguishing feature of coronaviruses and might play an essential role in the life cycle of this family of viruses. To examine these possibilities, we analyz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.804-812.1982
更新日期:1982-12-01 00:00:00
abstract::The coronavirus nucleocapsid (N) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. These functions are likely to be facilitated by interactions with host cell proteins. The potential interactome of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00321-13
更新日期:2013-09-01 00:00:00
abstract::The 5'-untranslated regions (5' UTRs) of picornavirus genomes contain an internal ribosomal entry site (IRES) that promotes the end-independent initiation of translation. Picornavirus IRESs are classified into four structurally distinct groups, each with different initiation factor requirements. Here, we identify a fi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05862-11
更新日期:2012-02-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 are closely related but pathogenically distinct human retroviruses. The antisense strand of the HTLV-1 genome encodes HTLV-1 basic leucine zipper (b-ZIP) protein (HBZ), a protein that inhibits Tax-mediated viral transcription, enhances T-cell proliferation, and pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00717-12
更新日期:2012-08-01 00:00:00
abstract::CaM kinase-Gr is a multifunctional Ca2+/calmodulin-dependent protein kinase which is enriched in neurons and T lymphocytes. The kinase is absent from primary human B lymphocytes but is expressed in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell lines, suggesting that expression of the kinase can be upregul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1697-1705.1994
更新日期:1994-03-01 00:00:00
abstract::Protective immunity to rotavirus (RV) is primarily mediated by antibodies produced by RV-specific memory B cells (RV-mBc). Of note, most of these cells express IgM, but the function of this subset is poorly understood. Here, using limiting dilution assays of highly sort-purified human IgM(+) mBc, we found that 62% and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01466-12
更新日期:2012-10-01 00:00:00
abstract::Vesicular stomatitis virus (VSV) vectors that express heterologous antigens have shown promise as vaccines in preclinical studies. The efficacy of VSV-based vaccines can be improved by engineering vectors that enhance innate immune responses. We previously generated a VSV vaccine vector that incorporates two enhancing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02898-13
更新日期:2014-01-01 00:00:00
abstract::Monoclonal antibodies (MAb) reactive with the glycoprotein of vesicular stomatitis virus (VSV) serotypes Indiana (VSV-Ind) and New Jersey (VSV-NJ) were used to protect mice against lethal infection. MAb which reacted with a number of distinct epitopes and which could neutralize the virus in vitro could also protect ag...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.1.208-214.1984
更新日期:1984-07-01 00:00:00
abstract::Three plaque isolates of SV40 strain 777 and 1 plaque isolate of strain 776 were grown to high-titer stocks and serially passaged, undiluted, in monkey BS-C-1 cells. In each case, the serial passaging procedure resulted in the accumulation of closed-circular SV40 DNA molecules containing covalently linked sequences ho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.492-500.1973
更新日期:1973-09-01 00:00:00
abstract::A vigorous expansion of antigen-specific CD8(+) T cells lacking apparent effector function was observed in a rhesus macaque acutely infected with the simian immunodeficiency virus (SIV) strain SIVmac239. Antigen-specific CD8(+) T cells were identified using antigenic-peptide class I major histocompatibility complex te...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.6.3028-3033.2001
更新日期:2001-03-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) envelope glycoprotein D (gD) plays an essential role in viral entry. The functional regions of gD responsible for viral entry have been mapped to its extracellular domain, whereas the gD cytoplasmic domain plays no obvious role in viral entry. Thus far, the role(s) of the gD cytoplasmic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01396-16
更新日期:2016-10-28 00:00:00
abstract::We isolated revertants of a polyomavirus whose origin of DNA replication contains a point mutation in the palindrome to which large T antigen binds. Four independent second-site revertants contain an Asp-286----Asn-286 substitution in large T antigen. This mutant large T antigen activates replication of DNAs containin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.242-249.1989
更新日期:1989-01-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) immediate-early (IE) regulatory protein infected-cell protein 0 (ICP0) is a strong and global transactivator of both viral and cellular genes. In a previous study, we reported that ICP0 is highly phosphorylated and contains at least seven distinct phosphorylation signals as dete...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1232-1243.2005
更新日期:2005-01-01 00:00:00