当前位置： SCI文献检索 > JOURNAL OF VIROLOGY期刊下所有文献 > Comparison of genotypes I and III in Japanese encephalitis virus reveals distinct differences in their genetic and host diversity.

Comparison of genotypes I and III in Japanese encephalitis virus reveals distinct differences in their genetic and host diversity.

Abstract：

UNLABELLED:Japanese encephalitis (JE) is an arthropod-borne disease associated with the majority of viral encephalitis cases in the Asia-Pacific region. The causative agent, Japanese encephalitis virus (JEV), has been phylogenetically divided into five genotypes. Recent surveillance data indicate that genotype I (GI) is gradually replacing genotype III (GIII) as the dominant genotype. To investigate the mechanism behind the genotype shift and the potential consequences in terms of vaccine efficacy, human cases, and virus dissemination, we collected (i) all full-length and partial JEV molecular sequences and (ii) associated genotype and host information comprising a data set of 873 sequences. We then examined differences between the two genotypes at the genetic and epidemiological level by investigating amino acid mutations, positive selection, and host range. We found that although GI is dominant, it has fewer sites predicted to be under positive selection, a narrower host range, and significantly fewer human isolates. For the E protein, the sites under positive selection define a haplotype set for each genotype that shows striking differences in their composition and diversity, with GIII showing significantly more variety than GI. Our results suggest that GI has displaced GIII by achieving a replication cycle that is more efficient but is also more restricted in its host range. IMPORTANCE:Japanese encephalitis is an arthropod-borne disease associated with the majority of viral encephalitis cases in the Asia-Pacific region. The causative agent, Japanese encephalitis virus (JEV), has been divided into five genotypes based on sequence similarity. Recent data indicate that genotype I (GI) is gradually replacing genotype III (GIII) as the dominant genotype. Understanding the reasons behind this shift and the potential consequences in terms of vaccine efficacy, human cases, and virus dissemination is important for controlling the spread of the virus and reducing human fatalities. We collected all available full-length and partial JEV molecular sequences and associated genotype and host information. We then examined differences between the two genotypes at the genetic and epidemiological levels by investigating amino acid mutations, positive selection, and host range. Our results suggest that GI has displaced GIII by achieving a replication cycle that is more efficient but more restricted in host range.

journal_name

J Virol

journal_title

Journal of virology

authors

Han N,Adams J,Chen P,Guo ZY,Zhong XF,Fang W,Li N,Wen L,Tao XY,Yuan ZM,Rayner S

doi

10.1128/JVI.02050-14

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

11469-79

issue

eissn

0022-538X

issn

1098-5514

pii

JVI.02050-14

journal_volume

pub_type

杂志文章

在线工具

Comparison of genotypes I and III in Japanese encephalitis virus reveals distinct differences in their genetic and host diversity.