Abstract:
:During studies of APOBEC3 (A3) anti-human immunodeficiency virus type 1 (anti-HIV-1) mechanisms, we identified a single cysteine at position 320 (C320) that disrupts A3DE activity. This residue is located in the recently identified DNA binding domain in A3G. Replacing C320 with a corresponding tyrosine from A3F (Y307) increased A3DE antiviral activity more than 20-fold. Conversely, replacing A3F Y307 with a cysteine or inserting a similar cysteine into A3B or A3G disrupted the anti-HIV activity of A3. Further investigation uncovered that C320 significantly reduces A3DE catalytic activity.
journal_name
J Viroljournal_title
Journal of virologyauthors
Dang Y,Abudu A,Son S,Harjes E,Spearman P,Matsuo H,Zheng YHdoi
10.1128/JVI.00243-11subject
Has Abstractpub_date
2011-06-01 00:00:00pages
5691-5issue
11eissn
0022-538Xissn
1098-5514pii
JVI.00243-11journal_volume
85pub_type
杂志文章abstract::The assembly and budding of Sindbis virus, a prototypic member of the alphavirus subgroup in the family Togaviridae, requires a specific interaction between the nucleocapsid core and the membrane-embedded glycoproteins E1 and E2. These glycoproteins are modified posttranslationally by the addition of palmitic acid, an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2546-2551.1993
更新日期:1993-05-01 00:00:00
abstract::After uptake into target cells, many nonenveloped viruses undergo conformational changes in the low-pH environment of the endocytic compartment. This results in exposure of amphipathic viral peptides and/or hydrophobic protein domains that are inserted into and either disrupt or perforate the vesicular membranes. The ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00329-11
更新日期:2011-08-01 00:00:00
abstract::Six phages were isolated from sewage and from a lysogenic strain. Three of them, belonging to a new morphological group, had contractile tails and elongated heads with axial ratios of 2.4:1. Two phages, possessing short tails and very long heads with axial ratios of 3.5:1, were new isolates of an extremely rare group....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.706-711.1974
更新日期:1974-03-01 00:00:00
abstract::Nectin-1 and nectin-2 are related molecules that can function with different specificities as entry receptors for mammalian alphaherpesviruses through interaction with viral glycoprotein D (gD). The normal function of members of the nectin family is to mediate cell-cell adhesion through homotypic and heterotypic necti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.12940-12950.2002
更新日期:2002-12-01 00:00:00
abstract::Infection of macaque monkeys with simian immunodeficiency virus (SIV) is probably the best animal model currently available for studying acquired immunodeficiency syndrome. In this report, we describe three infectious molecular clones of SIVmac and one of human immunodeficiency virus type 2 (HIV-2) and their use in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4691-4696.1988
更新日期:1988-12-01 00:00:00
abstract::We have amplified by the polymerase chain reaction, cloned, and sequenced genomic segments of 118 human papillomavirus type 16 (HPV-16) isolates from 76 cervical biopsy, 14 cervical smear, 3 vulval biopsy, 2 penile biopsy, 2 anal biopsy, and 1 vaginal biopsy sample and two cell lines. The specimens were taken from pat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2057-2066.1992
更新日期:1992-04-01 00:00:00
abstract:UNLABELLED:During hepatitis C virus (HCV) infection, broadly neutralizing antibody (bNAb) responses targeting E1E2 envelope glycoproteins are generated in many individuals. It is unclear if these antibodies play a protective or a pathogenic role during chronic infection. In this study, we investigated whether bNAb resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02669-15
更新日期:2016-04-14 00:00:00
abstract::The retroviral primary transcription product is a multifunctional RNA that is utilized as pre-mRNA, mRNA, and genomic RNA. The relationship between human immunodeficiency virus type 1 (HIV-1) unspliced transcripts used as mRNA for viral protein synthesis and as virion precursor RNA (vpRNA) for encapsidation remains an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11531-11537.2000
更新日期:2000-12-01 00:00:00
abstract::Mucosal surfaces are the primary portals for human immunodeficiency virus (HIV) transmission. Because systemic immunization, in general, does not induce effective mucosal immune responses, a mucosal HIV vaccine is urgently needed. For this study, we developed papillomavirus pseudoviruses that express HIV-1 Gag. The ps...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10249-10257.2004
更新日期:2004-10-01 00:00:00
abstract::The foot-and-mouth disease virus (FMDV) capsid is highly acid labile, but introduction of amino acid replacements, including an N17D change in VP1, can increase its acid resistance. Using mutant VP1 N17D as a starting point, we isolated a virus with higher acid resistance carrying an additional replacement, VP2 H145Y,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03222-13
更新日期:2014-03-01 00:00:00
abstract::Tankyrase (TNKS) is a telomere-associated poly-ADP ribose polymerase (PARP) that has been implicated along with several telomere repeat binding factors in the regulation of Epstein-Barr virus origin of plasmid replication (OriP). We now show that TNKS1 can bind to the family of repeats (FR) and dyad symmetry regions o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.4640-4650.2005
更新日期:2005-04-01 00:00:00
abstract::Dendritic cells (DCs) play a central role in instructing antiviral immune responses. DCs, however, can become targeted by different viruses themselves. We recently demonstrated that human DCs can be productively infected with echoviruses (EVs), but not coxsackie B viruses (CVBs), both of which are RNA viruses belongin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02376-07
更新日期:2008-03-01 00:00:00
abstract::Previous analyses have demonstrated that adenovirus DNA is packaged into virions in vivo in a polar, left-to-right fashion. The packaging of viral DNA is dependent on cis-acting elements at the left end of the genome. In this report, we describe a genetic analysis of the sequences that are required for efficient packa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2047-2056.1990
更新日期:1990-05-01 00:00:00
abstract::Hepatitis C virus (HCV) has two envelope proteins, E1 and E2, which form a heterooligomer. During dissection of interacting regions of HCV E1 and E2, we found the presence of an interfering compound or compounds in skim milk. Here we report that human as well as bovine lactoferrin, a multifunctional immunomodulator, b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.5997-6002.1997
更新日期:1997-08-01 00:00:00
abstract::The active site serine of the acetylesterase of influenza C virus was localized to amino acid 71 of the hemagglutinin-esterase protein by affinity labeling with 3H-labeled diisopropylfluorophosphate. This serine and the adjacent amino acids (Phe-Gly-Asp-Ser) are part of a consensus sequence motif found in serine hydro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2056-2062.1989
更新日期:1989-05-01 00:00:00
abstract::The nodavirus flock house virus (FHV) has a bipartite, positive-sense, RNA genome that encodes the catalytic subunit of the RNA replicase and the viral capsid protein precursor on separate genomic segments (RNA1 and RNA2, respectively). RNA1 can replicate autonomously when transfected into permissive cells, allowing s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.7933-7942.1999
更新日期:1999-10-01 00:00:00
abstract::Incubation of highly purified adeno-associated virus (AAV) capsids in vitro at pH 5.5 induced significant autocleavage of capsid proteins at several amino acid positions. No autocleavage was seen at pH 7.5. Examination of other AAV serotypes showed at least two different pH-induced cleavage patterns, suggesting that d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01717-12
更新日期:2012-11-01 00:00:00
abstract::Interleukin-1 receptor antagonist (IL-1ra) gene polymorphisms in 83 human immunodeficiency virus (HIV)-seropositive women were evaluated. Fourteen of the subjects (16.9%) were homozygous for IL-1ra allele 2 (IL-1RN*2). These women had a lower median level of HIV RNA than did women homozygous for allele 1 (IL-1RN*1) (P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.6242-6244.2001
更新日期:2001-07-01 00:00:00
abstract::The Middle East respiratory syndrome coronavirus (MERS-CoV) nonstructural protein 16 (nsp16) is an S-adenosyl-l-methionine (SAM)-dependent 2'-O-methyltransferase (2'-O-MTase) that is thought to methylate the ribose 2'-OH of the first transcribed nucleotide (N1) of viral RNA cap structures. This 2'-O-MTase activity is ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02217-16
更新日期:2017-02-14 00:00:00
abstract::The buoyant density of hepatitis C virus (HCV), with high in vivo infectivity (strain H) or low in vivo infectivity (strain F), was determined by sucrose gradient equilibrium centrifugation. Viral RNA of strain H was detected in fractions with densities of < or = 1.09 g/ml (principally approximately 1.06 g/ml), while ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.1953-1958.1993
更新日期:1993-04-01 00:00:00
abstract::Arenaviruses are rodent-borne viruses, with five members of the family capable of causing severe hemorrhagic fevers if transmitted to humans. To date, two distinct cellular receptors have been identified that are used by different pathogenic viruses, alpha-dystroglycan by Lassa fever virus and transferrin receptor 1 (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01397-07
更新日期:2008-01-01 00:00:00
abstract::Viruses with spindle-shaped virions are abundant in diverse environments. Over the years, such viruses have been isolated from a wide range of archaeal hosts. Evolutionary relationships between them remained enigmatic, however. Here, using structural proteins as markers, we define familial ties among these "dark horse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02941-13
更新日期:2014-02-01 00:00:00
abstract::AV3 cells (continuous human amnion) infected with the Onderstepoort strain of canine distemper virus produced cell fusion within 2 to 5 hr when added to AV3 cell monolayers. An apparent requirement for intact, infected cells was demonstrated by showing that (i) frozen-and-thawed infected cells failed to induce fusion,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.6.1179-1183.1972
更新日期:1972-12-01 00:00:00
abstract::Type C RNA virus(es) was readily released from primary lymphosarcoma cell cultures of WH/J rabbits after induction with halogenated pyrimidines. The virus contained an RNA-directed DNA polymerase and the p30 structural protein. The rabbit virus RNA-directed DNA polymerase and p30 protein shared antigenic homologies wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.2.313-319.1978
更新日期:1978-08-01 00:00:00
abstract::While cytomegalovirus (CMV) infections are often limited in host range by lengthy coevolution with a single host species, a few CMVs are known to deviate from this rule. For example, rhesus macaque CMV (RhCMV), a model for human CMV (HCMV) pathogenesis and vaccine development, can replicate in human cells, as well as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01793-17
更新日期:2018-02-26 00:00:00
abstract::Intracisternal-A-particle-related envelope-encoding (IAPE) proviral elements in the mouse genome encode and express an envelope-like protein that may allow transmission of IAPEs as infectious agents. To test IAPE mobility and potential transmission in mice, we have analyzed the distribution of IAPE elements in the gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6450-6454.1996
更新日期:1996-09-01 00:00:00
abstract:UNLABELLED:Little is known about the antiviral response in mollusks. As in other invertebrates, the interferon signaling pathways have not been identified, and in fact, there is a debate about whether invertebrates possess antiviral immunity similar to that of vertebrates. In marine bivalves, due to their filtering act...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00591-16
更新日期:2016-08-12 00:00:00
abstract::Human coronavirus NL63 (HCoV-NL63), a common human respiratory pathogen, is associated with both upper and lower respiratory tract disease in children and adults. Currently, no antiviral drugs are available to treat CoV infections; thus, potential drug targets need to be identified and characterized. Here, we identify...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02747-06
更新日期:2007-06-01 00:00:00
abstract::The vaccinia virus (VV) A10L gene codes for a major core protein, P4a. This polypeptide is synthesized at late times during viral infection and is proteolytically cleaved during virion assembly. To investigate the role of P4a in the virus life cycle and morphogenesis, we have generated an inducer-dependent conditional...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5778-5795.2001
更新日期:2001-07-01 00:00:00
abstract::Borna disease virus infection is diagnosed by the presence of serum antibodies reactive with the major viral proteins, p40 and p23. Although p40 and p23 are unrelated in amino acid sequence structure, cross-reactive antibodies are described. Protein fragments and synthetic peptides were analyzed to characterize the sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8133-8137.1996
更新日期:1996-11-01 00:00:00