Induction and maintenance of autonomous flock house virus RNA1 replication.


:The nodavirus flock house virus (FHV) has a bipartite, positive-sense, RNA genome that encodes the catalytic subunit of the RNA replicase and the viral capsid protein precursor on separate genomic segments (RNA1 and RNA2, respectively). RNA1 can replicate autonomously when transfected into permissive cells, allowing study of the kinetics of RNA1 replication in the absence of either RNA2 or capsid proteins. However, RNA1 replication ceases ca. 3 days after transfection despite the presence of replication-competent RNA. We examined this inhibition by inducing the expression of RNA1 in cells from a cDNA copy that was under the control of a hormone-regulated RNA polymerase II promoter. This system reproduced the shutoff of RNA replication when DNA-templated primary transcription was turned off. Continued primary transcription partially alleviated the shutoff and maintained the rate of RNA replication for several days at a steady-state level approximately one-third that of the peak rate. After shutoff, RNA replication could be restored by transferring the resulting intracellular RNA to fresh cells or by reinducing primary transcription, indicating that cessation of replication occurred despite the competence of both the viral RNA and the cytoplasmic environment. These data suggest that there is a mechanism by which replication is shut off at late times after transfection, which may reflect the natural endpoint of the replicative cycle.


J Virol


Journal of virology


Johnson KL,Ball LA




Has Abstract


1999-10-01 00:00:00












  • Cross-reactivity between hepatitis C virus and Influenza A virus determinant-specific cytotoxic T cells.

    abstract::The cellular immune response contributes to viral clearance as well as to liver injury in acute and chronic hepatitis C virus (HCV) infection. An immunodominant determinant frequently recognized by liver-infiltrating and circulating CD8(+) T cells of HCV-infected patients is the HCV(NS3-1073) peptide CVNGVCWTV. Using ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wedemeyer H,Mizukoshi E,Davis AR,Bennink JR,Rehermann B

    更新日期:2001-12-01 00:00:00

  • Requirements for RNA replication of a poliovirus replicon by coxsackievirus B3 RNA polymerase.

    abstract::A chimeric poliovirus type 1 (PV1) genome was constructed in which the 3D RNA polymerase (3D(pol)) coding sequences were replaced with those from coxsackievirus B3 (CVB3). No infectious virus was produced from HeLa cells transfected with the chimeric RNA. Processing of the PV1 capsid protein precursor was incomplete, ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bell YC,Semler BL,Ehrenfeld E

    更新日期:1999-11-01 00:00:00

  • Baculovirus gene ME53, which contains a putative zinc finger motif, is one of the major early-transcribed genes.

    abstract::We have been investigating the first viral genes to be transcribed in Spodoptera frugiperda cells after the infection with Autographa california nuclear polyhedrosis virus in order to identify regulatory proteins required for the activation of early and/or late gene transcription. By screening a cDNA library from poly...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Knebel-Mörsdorf D,Kremer A,Jahnel F

    更新日期:1993-02-01 00:00:00

  • The majority of infiltrating CD8+ T cells in the central nervous system of susceptible SJL/J mice infected with Theiler's virus are virus specific and fully functional.

    abstract::Theiler's virus infection of the central nervous system (CNS) induces an immune-mediated demyelinating disease in susceptible mouse strains, such as SJL/J, and serves as a relevant infectious model for human multiple sclerosis. It has been previously suggested that susceptible SJL/J mice do not mount an efficient cyto...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kang BS,Lyman MA,Kim BS

    更新日期:2002-07-01 00:00:00

  • Polyamines contained by two densonucleosis viruses.

    abstract::Densonucleosis viruses 1 and 2 both contain the three polyamines putrescine, spermidine, and spermine in complete virus particles but not in their respective top components. The polyamines, with spermidine predominant, comprise 1.41% of the virus particle by weight, which is sufficient to neutralize 26% of the single-...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kelly DC,Elliott RM

    更新日期:1977-01-01 00:00:00

  • Variability of human immunodeficiency virus type 1 group O strains isolated from Cameroonian patients living in France.

    abstract::Human immunodeficiency virus type 1 (HIV-1) nucleotide sequences encoding p24Gag and the Env C2V3 region were obtained from seven patients who were selected on the basis of having paradoxical seronegativity on a subset of HIV enzyme-linked immunosorbent assay detection kits and having atypical Western blot (immunoblot...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Loussert-Ajaka I,Chaix ML,Korber B,Letourneur F,Gomas E,Allen E,Ly TD,Brun-Vézinet F,Simon F,Saragosti S

    更新日期:1995-09-01 00:00:00

  • RNA splicing in Borna disease virus, a nonsegmented, negative-strand RNA virus.

    abstract::Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus related to rhabdoviruses and paramyxoviruses. Unlike animal viruses of these two families, BDV transcribes RNAs in the nuclei of infected cells and produces high levels of transcripts containing multiple open reading frames. Previous Northern blot ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Schneider PA,Schneemann A,Lipkin WI

    更新日期:1994-08-01 00:00:00

  • Genetic mapping of reovirus virulence and organ tropism in severe combined immunodeficient mice: organ-specific virulence genes.

    abstract::We used reovirus reassortant genetics and severe combined immunodeficient (SCID) mice to define viral genes important for organ tropism and virulence in the absence of antigen-specific immunity. Adult SCID mice infected with reovirus serotype 1 strain Lang (T1L) died after 20 +/- 6 days, while infection with serotype ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Haller BL,Barkon ML,Vogler GP,Virgin HW 4th

    更新日期:1995-01-01 00:00:00

  • Circovirus transport proceeds via direct interaction of the cytoplasmic dynein IC1 subunit with the viral capsid protein.

    abstract:UNLABELLED:Microtubule transport of circovirus from the periphery of the cell to the nucleus is essential for viral replication in early infection. How the microtubule is recruited to the viral cargo remains unclear. In this study, we observed that circovirus trafficking is dependent on microtubule polymerization and t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Cao J,Lin C,Wang H,Wang L,Zhou N,Jin Y,Liao M,Zhou J

    更新日期:2015-03-01 00:00:00

  • Inhibition of p53-mediated transactivation by E6 of type 1, but not type 5, 8, or 47, human papillomavirus of cutaneous origin.

    abstract::Transient transfection experiments indicated (i) that E6 protein of non-cancer-associated cutaneous human papillomavirus type 1 (HPV-1) can inhibit p53-mediated transcriptional transactivation in both p53-deficient human cells (H358) and normal rat cells (3Y1), but those of cancer-associated cutaneous HPV-5, -8, and -...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kiyono T,Hiraiwa A,Ishii S,Takahashi T,Ishibashi M

    更新日期:1994-07-01 00:00:00

  • Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli.

    abstract::The 3C region of human rhinovirus 14 was expressed in Escherichia coli. The microbially synthesized protease was functional, since the expressed precursor underwent autoproteolytic processing to generate mature molecules of the expected molecular weight and antigenicity. Mutation of the putative active-site Cys-146 re...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Cordingley MG,Register RB,Callahan PL,Garsky VM,Colonno RJ

    更新日期:1989-12-01 00:00:00

  • Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus.

    abstract::We describe a modification of the polymerase chain reaction technique which allows amplification of cellular DNA adjacent to an integrated provirus given sequence information for the provirus only. The modified technique should be generally useful for studies of insertional mutagenesis and other situations in which on...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Silver J,Keerikatte V

    更新日期:1989-05-01 00:00:00

  • Mutational analysis of the cysteine residues in the hepatitis B virus small envelope protein.

    abstract::The small envelope protein of hepatitis B virus is the major component of the viral coat and is also secreted from cells as a 20-nm subviral particle, even in the absence of other viral proteins. Such empty envelope particles are composed of approximately 100 copies of this polypeptide and host-derived lipids and are ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mangold CM,Streeck RE

    更新日期:1993-08-01 00:00:00

  • Evidence that less-than-full-length pol gene products are functional in hepadnavirus DNA synthesis.

    abstract::Duck hepatitis B virus mutants containing frameshift or stop codon mutations in a portion of the viral pol gene separating the terminal protein and reverse transcriptase domains had a leaky phenotype and, depending on the location and type of mutation, synthesized up to 10% as much viral DNA as did the wild type. This...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wu TT,Condreay LD,Coates L,Aldrich C,Mason W

    更新日期:1991-05-01 00:00:00

  • Two signals mediate nuclear localization of influenza virus (A/WSN/33) polymerase basic protein 2.

    abstract::Polymerase basic protein 2 (PB2), a component of the influenza virus polymerase complex, when expressed alone from cloned cDNA in the absence of other influenza virus proteins, is transported into the nucleus. In this study, we have examined the nuclear translocation signal of PB2 by making deletions and mutations in ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mukaigawa J,Nayak DP

    更新日期:1991-01-01 00:00:00

  • NF-κB-Interacting Long Noncoding RNA Regulates HIV-1 Replication and Latency by Repressing NF-κB Signaling.

    abstract::NF-κB-interacting long noncoding RNA (NKILA) was recently identified as a negative regulator of NF-κB signaling and plays an important role in the development of various cancers. It is well known that NF-κB-mediated activation of human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR)-driven gene expres...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wang H,Liu Y,Huan C,Yang J,Li Z,Zheng B,Wang Y,Zhang W

    更新日期:2020-08-17 00:00:00

  • The human immunodeficiency virus type 1 capsid p2 domain confers sensitivity to the cyclophilin-binding drug SDZ NIM 811.

    abstract::Human immunodeficiency virus type 1 (HIV-1) specifically incorporates the host cell peptidyl-prolyl isomerase cyclophilin A into virions via contacts with the capsid (CA) domain of the Gag polyprotein Pr55gag. The immunosuppressant drug cyclosporin A and the nonimmunosuppressive cyclosporin A analog SDZ NIM 811 bind t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Dorfman T,Göttlinger HG

    更新日期:1996-09-01 00:00:00

  • Clearance of a productive lentivirus infection in calves experimentally inoculated with caprine arthritis-encephalitis virus.

    abstract::Lentiviruses have long been considered host-specific pathogens, but several recent observations demonstrated their capacity to conquer new hosts from different species, genera, and families. From these cross-species infections emerged new animal and human infectious diseases. The successful colonization and adaptation...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Morin T,Guiguen F,Bouzar BA,Villet S,Greenland T,Grezel D,Gounel F,Gallay K,Garnier C,Durand J,Alogninouwa T,Mselli-Lakhal L,Mornex JF,Chebloune Y

    更新日期:2003-06-01 00:00:00

  • Biochemical characterization of the equine arteritis virus helicase suggests a close functional relationship between arterivirus and coronavirus helicases.

    abstract::The arterivirus equine arteritis virus nonstructural protein 10 (nsp10) has previously been predicted to contain a Zn finger structure linked to a superfamily 1 (SF1) helicase domain. A recombinant form of nsp10, MBP-nsp10, was produced in Escherichia coli as a fusion protein with the maltose-binding protein. The prot...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Seybert A,van Dinten LC,Snijder EJ,Ziebuhr J

    更新日期:2000-10-01 00:00:00

  • Expression of the hepatitis B virus core gene in vitro and in vivo.

    abstract::The core gene of hepatitis B virus contains two in-phase AUG codons which may both be used in the viral life cycle. By in vitro translation of transcripts produced in vitro, we investigated the corresponding core gene products and their counterparts in vivo. Depending on the location of the 5' end of the transcripts, ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Weimer T,Salfeld J,Will H

    更新日期:1987-10-01 00:00:00

  • Strain variation of R5 direct repeats in the right-hand portion of the long unique segment of varicella-zoster virus DNA.

    abstract::We located a region of interstrain size variability in a short segment in an area at the right-hand end of the long unique sequence of the varicella-zoster viral genome. Varicella-zoster virus strains isolated in a district of Japan were classified into three groups on the basis of the size of this segment. Sequence c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hondo R,Yogo Y

    更新日期:1988-08-01 00:00:00

  • Evolution-Guided Structural and Functional Analyses of the HERC Family Reveal an Ancient Marine Origin and Determinants of Antiviral Activity.

    abstract::In humans, homologous to the E6-AP carboxyl terminus (HECT) and regulator of chromosome condensation 1 (RCC1)-like domain-containing protein 5 (HERC5) is an interferon-induced protein that inhibits replication of evolutionarily diverse viruses, including human immunodeficiency virus type 1 (HIV-1). To better understan...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Paparisto E,Woods MW,Coleman MD,Moghadasi SA,Kochar DS,Tom SK,Kohio HP,Gibson RM,Rohringer TJ,Hunt NR,Di Gravio EJ,Zhang JY,Tian M,Gao Y,Arts EJ,Barr SD

    更新日期:2018-06-13 00:00:00

  • Antagonism of the Protein Kinase R Pathway in Human Cells by Rhesus Cytomegalovirus.

    abstract::While cytomegalovirus (CMV) infections are often limited in host range by lengthy coevolution with a single host species, a few CMVs are known to deviate from this rule. For example, rhesus macaque CMV (RhCMV), a model for human CMV (HCMV) pathogenesis and vaccine development, can replicate in human cells, as well as ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Child SJ,Hickson SE,Bayer A,Malouli D,Früh K,Geballe AP

    更新日期:2018-02-26 00:00:00

  • Chromosomal locations and gonadal dependence of genes that mediate resistance to ectromelia (mousepox) virus-induced mortality.

    abstract::Four genetic loci were tested for linkage with loci that control genetic resistance to lethal ectromelia virus infection in mice. Three of the loci were selected because of concordance with genotypes assigned to recombinant inbred (RI) strains of mice derived from resistant C57BL/6 and susceptible DBA/2 (BXD) mice on ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Brownstein DG,Bhatt PN,Gras L,Jacoby RO

    更新日期:1991-04-01 00:00:00

  • Virion Structure of Iflavirus Slow Bee Paralysis Virus at 2.6-Angstrom Resolution.

    abstract:UNLABELLED:The western honeybee (Apis mellifera) is the most important commercial insect pollinator. However, bees are under pressure from habitat loss, environmental stress, and pathogens, including viruses that can cause lethal epidemics. Slow bee paralysis virus (SBPV) belongs to the Iflaviridae family of nonenvelop...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kalynych S,Přidal A,Pálková L,Levdansky Y,de Miranda JR,Plevka P

    更新日期:2016-07-27 00:00:00

  • Characterization of a bacteriophage related to R-type pyocins.

    abstract::A bacteriophage with a contractile tail which shows very similar features to R-type pyocins was isolated and characterized. This phage, named PS17,was purified by DEAE-cellulose chromatography and CsCl density gradient centrifugation. It was a DNA-containing phage, and the density of the purified particles in CsCl was...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kageyama M,Shinomiya T,Aihara Y,Kobayashi M

    更新日期:1979-12-01 00:00:00

  • Specific inhibition of the synthesis of human lysyl-tRNA synthetase results in decreases in tRNA(Lys) incorporation, tRNA(3)(Lys) annealing to viral RNA, and viral infectivity in human immunodeficiency virus type 1.

    abstract::The major human tRNA(Lys) isoacceptors, and, are selectively packaged into human immunodeficiency virus type 1 (HIV-1) during assembly, where acts as a primer for reverse transcription. Lysyl-tRNA synthetase (LysRS) is also incorporated into HIV-1, independently of tRNA(Lys), via its interaction with Gag, and is a str...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Guo F,Cen S,Niu M,Javanbakht H,Kleiman L

    更新日期:2003-09-01 00:00:00

  • Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1.

    abstract::The Zaire ebolavirus protein VP24 was previously demonstrated to inhibit alpha/beta interferon (IFN-alpha/beta)- and IFN-gamma-induced nuclear accumulation of tyrosine-phosphorylated STAT1 (PY-STAT1) and to inhibit IFN-alpha/beta- and IFN-gamma-induced gene expression. These properties correlated with the ability of V...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Reid SP,Valmas C,Martinez O,Sanchez FM,Basler CF

    更新日期:2007-12-01 00:00:00

  • Protein phosphatase 2A dephosphorylates simian virus 40 large T antigen specifically at residues involved in regulation of DNA-binding activity.

    abstract::Treatment of purified simian virus 40 large T antigen (LT) with protein phosphatase 2A stimulates LT-dependent DNA unwinding and replication (D. M. Virshup, M. G. Kauffman, and T. J. Kelly, EMBO J. 8: 3891-3898, 1989). The specificity of the catalytic subunit of protein phosphatase 2A toward LT was investigated by two...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Scheidtmann KH,Virshup DM,Kelly TJ

    更新日期:1991-04-01 00:00:00

  • Marker rescue of temperature-sensitive mutations of vaccinia virus WR: correlation of genetic and physical maps.

    abstract::The physical map locations of 62 temperature-sensitive mutations of vaccinia virus WR have been determined by marker rescue experiments, using cloned HindIII fragments of wild-type DNA. Since vaccinia virus DNA is not infectious, marker rescue was performed by infecting monolayers of cells at the nonpermissive tempera...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ensinger MJ,Rovinsky M

    更新日期:1983-11-01 00:00:00