Abstract:
:The nodavirus flock house virus (FHV) has a bipartite, positive-sense, RNA genome that encodes the catalytic subunit of the RNA replicase and the viral capsid protein precursor on separate genomic segments (RNA1 and RNA2, respectively). RNA1 can replicate autonomously when transfected into permissive cells, allowing study of the kinetics of RNA1 replication in the absence of either RNA2 or capsid proteins. However, RNA1 replication ceases ca. 3 days after transfection despite the presence of replication-competent RNA. We examined this inhibition by inducing the expression of RNA1 in cells from a cDNA copy that was under the control of a hormone-regulated RNA polymerase II promoter. This system reproduced the shutoff of RNA replication when DNA-templated primary transcription was turned off. Continued primary transcription partially alleviated the shutoff and maintained the rate of RNA replication for several days at a steady-state level approximately one-third that of the peak rate. After shutoff, RNA replication could be restored by transferring the resulting intracellular RNA to fresh cells or by reinducing primary transcription, indicating that cessation of replication occurred despite the competence of both the viral RNA and the cytoplasmic environment. These data suggest that there is a mechanism by which replication is shut off at late times after transfection, which may reflect the natural endpoint of the replicative cycle.
journal_name
J Viroljournal_title
Journal of virologyauthors
Johnson KL,Ball LAdoi
10.1128/JVI.73.10.7933-7942.1999subject
Has Abstractpub_date
1999-10-01 00:00:00pages
7933-42issue
10eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::The matrix (M) protein of vesicular stomatitis virus (VSV) is a potent inhibitor in vivo of transcription by all three host RNA polymerases (RNAP). In the case of host RNA polymerase II (RNAPII), the inhibition is due to lack of activity of the TATA-binding protein (TBP), which is a subunit of the basal transcription ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4453-4458.2001
更新日期:2001-05-01 00:00:00
abstract::In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02339-07
更新日期:2008-02-01 00:00:00
abstract::A series of plasmids containing hepatitis A virus (HAV) cDNA was constructed such that positive-strand HAV RNA could be transcribed with T7 RNA polymerase. The plasmids differed in the number of 5'-terminal nucleotides representing the junctions between vectors and HAV sequences that were present in the transcripts. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2757-2760.1991
更新日期:1991-05-01 00:00:00
abstract::The human immunodeficiency virus type 1 Vpu protein is a 16-kDa phosphoprotein which enhances the efficiency of virion production and induces rapid degradation of CD4, the cellular receptor for human immunodeficiency virus. The topology of membrane-inserted Vpu was investigated by using in vitro-synthesized Vpu cotran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.5056-5061.1993
更新日期:1993-08-01 00:00:00
abstract::Virus-infected cells secrete a broad range of interferons (IFN) which confer resistance to yet uninfected cells by triggering the synthesis of antiviral factors. The relative contributions of the various IFN subtypes to innate immunity against virus infections remain elusive. IFN-alpha, IFN-beta, and other type I IFN ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00272-10
更新日期:2010-06-01 00:00:00
abstract::Replication of flock house virus (FHV) RNA1 and production of subgenomic RNA3 in the yeast Saccharomyces cerevisiae provide a useful tool for the dissection of FHV molecular biology and host-encoded functions involved in RNA replication. The replication template activity of RNA1 can be separated from its coding potent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3905-3919.2002
更新日期:2002-04-01 00:00:00
abstract::A cloned DNA copy of simian rotavirus SA11 genomic segment 10 was used to confirm the assignment of the nonstructural glycoprotein NCVP5 to this gene. Determination of the nucleotide sequence for gene 10 indicated that NCVP5 is 175 amino acids in length and has an N-terminal hydrophobic region with the characteristics...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.2.335-339.1983
更新日期:1983-11-01 00:00:00
abstract::Rfv3 is an autosomal dominant gene that influences the recovery of resistant mice from Friend retrovirus (FV) infection by limiting viremia and promoting a more potent neutralizing antibody response. We previously reported that Rfv3 is encoded by Apobec3, an innate retrovirus restriction factor. However, it was recent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01838-10
更新日期:2011-01-01 00:00:00
abstract::During viral infection, inflammation and recovery are tightly controlled by competing proinflammatory and regulatory immune pathways. Respiratory syncytial virus (RSV) is the leading global cause of infantile bronchiolitis, which is associated with recurrent wheeze and asthma diagnosis in later life. Th2-driven diseas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01295-13
更新日期:2013-10-01 00:00:00
abstract::RNA plant viruses use various translational regulatory mechanisms to control their gene expression. Translational enhancement of viral mRNAs that leads to higher levels of protein synthesis from specific genes may be essential for the virus to successfully compete for cellular translational machinery. The control elem...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1144-1153.2002
更新日期:2002-02-01 00:00:00
abstract::The complement pathway is involved in eliminating antigen immune complexes. However, the role of the C3 complement system remains largely unknown in influenza virus M2 extracellular (M2e) domain or hemagglutinin (HA) vaccine-mediated protection after vaccination. Using a C3 knockout (C3 KO) mouse model, we found that ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00969-18
更新日期:2018-09-26 00:00:00
abstract::The need to replace rabies immune globulin (RIG) as an essential component of rabies postexposure prophylaxis is widely acknowledged. We set out to discover a unique combination of human monoclonal antibodies (MAbs) able to replace RIG. Stringent criteria concerning neutralizing potency, affinity, breadth of neutraliz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.9062-9068.2005
更新日期:2005-07-01 00:00:00
abstract::Murine norovirus (MNV), a prevalent pathogen of laboratory mice, shares many characteristics with human noroviruses. Previous results indicated that passage of MNV1 in the macrophage cell line RAW 264.7 results in attenuation in STAT1-deficient mice (C. E. Wobus, S. M. Karst, L. B. Thackray, K. O. Chang, S. V. Sosnovt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00237-08
更新日期:2008-08-01 00:00:00
abstract::The affinity of human immunodeficiency virus (HIV) envelope for CD4 and CCR5 appears to be associated with aspects of R5 virus (virus using the CCR5 coreceptor) pathogenicity. However, entry efficiency results from complex interactions between the viral envelope glycoprotein and both CD4 and CCR5, which limits attempt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01242-09
更新日期:2009-11-01 00:00:00
abstract::Activation of the interferon-inducible, double-stranded RNA-dependent protein kinase was monitored in monolayer cultures of control and interferon-treated HeLa cells infected with encephalomyocarditis virus. The extent of phosphorylation in the intact cell of the alpha-subunit of eucaryotic protein synthesis initiatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.3.894-898.1985
更新日期:1985-06-01 00:00:00
abstract::We have developed a system in which vesicular stomatitis virus (VSV) minigenomes encoding viral structural proteins can be expressed from plasmids. These RNAs can be replicated, transcribed, and packaged into infectious particles when coexpressed with the other VSV proteins. The minigenomes contain either the glycopro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.2946-2953.1995
更新日期:1995-05-01 00:00:00
abstract::Synthesis of cytoplasmic viral RNA was studied during infection of cultured human (KB) cells with adenovirus 2. At 6 h, before viral DNA synthesis began 5% of the poly(A)-containing RNA hybridized to viral DNA; by 12 h and at later times more than 80% was virus specified. At 18 h after infection, four major size class...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.137-144.1975
更新日期:1975-01-01 00:00:00
abstract::Infection of newborn Lewis rats with Borna disease virus (neonatal Borna disease [NBD]) results in cerebellar damage without the cellular inflammation associated with infections in later life. Purkinje cell (PC) damage has been reported for several models of early-life viral infection, including NBD; however, the time...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02245-06
更新日期:2007-03-01 00:00:00
abstract::The vast majority of studies with candidate immunogens based on the human immunodeficiency virus envelope (Env) have been conducted with Env proteins derived from clade B viruses isolated during chronic infection. Whether non-clade B Env protein immunogens will elicit antibodies with epitope specificities that are sim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00389-08
更新日期:2008-06-01 00:00:00
abstract::We have used a replication-competent shuttle vector based on the genome of Rous sarcoma virus to characterize genomic rearrangements that occur during retrovirus replication. The strategy involved cloning circular DNA that was generated during an acute infection. While analyzing a class of retroviral DNA clones that a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5735-5743.1992
更新日期:1992-10-01 00:00:00
abstract::Human cytomegalovirus (HCMV) UL84 is a phosphoprotein that shuttles from the nucleus to the cytoplasm and is required for oriLyt-dependent DNA replication and viral growth. UL84 was previously shown to interact with IE2 (IE86) in infected cells, and this interaction down-regulates IE2-mediated transcriptional activati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-07
更新日期:2008-01-01 00:00:00
abstract::Mice that express the viral oncoprotein simian virus 40 (SV40) large T antigen (T-Ag) as a transgene provide useful models for the assessment of the state of the host immune response in the face of spontaneous tumor progression. Line SV11 (H2(b)) mice develop rapidly progressing choroid plexus tumors due to expression...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1751-1762.2004
更新日期:2004-02-01 00:00:00
abstract::We have used immunofluorescence in parallel with transmission and scanning electron microscopy to characterize the unusual cytoplasmic and nucleolar accumulation of Simian virus 40 (SV40) virion protein (C antigen) at restrictive temperatures (39 to 41 C) in monkey cells infected with a temperature-sensitive mutant of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.6.1530-1546.1974
更新日期:1974-12-01 00:00:00
abstract::The presence of a high number of activated T cells in the bloodstream and spontaneous proliferation of peripheral blood mononuclear cells in vitro are striking characteristics of human T-cell leukemia virus type I (HTLV-I) infection. The HTLV-I regulatory protein Tax and the envelope protein gp46 have been implicated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3134-3141.1993
更新日期:1993-06-01 00:00:00
abstract::NYVAC has been engineered as a safe, attenuated vaccinia virus (VV) vector for use in vaccination against a broad spectrum of pathogens and tumors. Due to the interest in NYVAC-based vectors as vaccines and current phase I/II clinical trials with this vector, there is a need to analyze the human host response to NYVAC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.985-998.2006
更新日期:2006-01-01 00:00:00
abstract:UNLABELLED:Identification and characterization of CD8(+) T cells effectively controlling HIV-1 variants are necessary for the development of AIDS vaccines and for studies of AIDS pathogenesis, although such CD8(+) T cells have been only partially identified. In this study, we sought to identify CD8(+) T cells controlli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00020-15
更新日期:2015-05-01 00:00:00
abstract::The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a possible influence of these proteins on replication and pathogenesis and about the mechanism of virus formation could not...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.4008-4021.2002
更新日期:2002-04-01 00:00:00
abstract::A chimeric poliovirus type 1 (PV1) genome was constructed in which the 3D RNA polymerase (3D(pol)) coding sequences were replaced with those from coxsackievirus B3 (CVB3). No infectious virus was produced from HeLa cells transfected with the chimeric RNA. Processing of the PV1 capsid protein precursor was incomplete, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9413-9421.1999
更新日期:1999-11-01 00:00:00
abstract::Langat virus (LGT), the naturally attenuated member of the tick-borne encephalitis virus (TBEV) complex, was tested extensively in clinical trials as a live TBEV vaccine and was found to induce a protective, durable immune response; however, it retained a low residual neuroinvasiveness in mice and humans. In order to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1427-1439.2006
更新日期:2006-02-01 00:00:00
abstract::Some paramyxovirus V proteins induce STAT protein degradation, and the amino acids essential for this process in the human parainfluenza virus type 2 (hPIV2) V protein have been studied. Various recombinant hPIV2s and cell lines constitutively expressing various mutant V proteins were generated. We found that V protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8591-8601.2005
更新日期:2005-07-01 00:00:00