Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus.

abstract：:Human MxA protein accumulates in the cytoplasm of interferon-treated cells and inhibits the multiplication of several RNA viruses, including Thogoto virus (THOV), a tick-borne orthomyxovirus that transcribes and replicates its genome in the cell nucleus. The antiviral mechanism of MxA was investigated by using two alt...

journal_title：Journal of virology

authors： Weber F,Haller O,Kochs G

更新日期：2000-01-01 00:00:00

abstract：:The ascomycete Botrytis porri causes clove rot and leaf blight of garlic worldwide. We report here the biological and molecular features of a novel bipartite double-stranded RNA (dsRNA) mycovirus named Botrytis porri RNA virus 1 (BpRV1) from the hypovirulent strain GarlicBc-72 of B. porri. The BpRV1 genome comprises t...

journal_title：Journal of virology

authors： Wu M,Jin F,Zhang J,Yang L,Jiang D,Li G

更新日期：2012-06-01 00:00:00

abstract：:Signal peptidase cleavage at the C-prM junction in the flavivirus structural polyprotein is inefficient in the absence of the cytoplasmic viral protease, which catalyzes cleavage at the COOH terminus of the C protein. The signal peptidase cleavage occurs efficiently in circumstances where the C protein is deleted or i...

journal_title：Journal of virology

authors： Stocks CE,Lobigs M

更新日期：1998-03-01 00:00:00

abstract：:Deoxyribonucleoside triphosphates are incorporated into T4 DNA in infected cells treated with toluene. Under the proper conditions the incorporation is controlled by the known T4 DNA polymerase and proceeds by a semiconservative mechanism. Both strands of the phage DNA are replicated into a high molecular weight proge...

journal_title：Journal of virology

authors： Miller RC Jr,Taylor DM,MacKay K,Smith HW

更新日期：1973-12-01 00:00:00

abstract：:Some picornaviruses, for example, poliovirus, increase bidirectional permeability of the nuclear envelope and suppress active nucleocytoplasmic transport. These activities require the viral protease 2A(pro). Here, we studied nucleocytoplasmic traffic in cells infected with encephalomyocarditis virus (EMCV; a cardiovir...

journal_title：Journal of virology

authors： Lidsky PV,Hato S,Bardina MV,Aminev AG,Palmenberg AC,Sheval EV,Polyakov VY,van Kuppeveld FJ,Agol VI

更新日期：2006-03-01 00:00:00

abstract：:The majority of double-stranded DNA (dsDNA) viruses infecting eukaryotic organisms use host- or virus-expressed histones or protamine-like proteins to condense their genomes. In contrast, members of the Baculoviridae family use a protamine-like protein named P6.9. The dephosphorylated form of P6.9 binds to DNA in a no...

journal_title：Journal of virology

authors： Wang M,Tuladhar E,Shen S,Wang H,van Oers MM,Vlak JM,Westenberg M

更新日期：2010-09-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) ICP34.5 gene is a neurovirulence gene in mice. In addition, some ICP34.5 mutants have been reported to have a reduced efficiency of induced reactivation as measured by in vitro explantation of latently infected mouse ganglia. However, since spontaneous reactivation is almost non...

journal_title：Journal of virology

authors： Perng GC,Thompson RL,Sawtell NM,Taylor WE,Slanina SM,Ghiasi H,Kaiwar R,Nesburn AB,Wechsler SL

更新日期：1995-05-01 00:00:00

abstract：:Innate immunity provides an immediate defense against infection after host cells sense danger signals from microbes. Endoplasmic reticulum (ER) stress arises from accumulation of misfolded/unfolded proteins when protein load overwhelms the ER folding capacity, which activates the unfolded protein response (UPR) to res...

journal_title：Journal of virology

authors： Frabutt DA,Wang B,Riaz S,Schwartz RC,Zheng YH

更新日期：2017-12-14 00:00:00

abstract：:The human papillomavirus (HPV) E6 oncoproteins recruit the cellular ubiquitin ligase E6AP/UBE3A to target cellular substrates for proteasome-mediated degradation, and one consequence of this activity is the E6 stimulation of E6AP autoubiquitination and degradation. Recent studies identified an autism-linked mutation w...

journal_title：Journal of virology

authors： Thatte J,Banks L

更新日期：2017-10-27 00:00:00

abstract：:A pediatric human immunodeficiency virus type 1 (HIV-1) vaccine would be desirable to protect infants against HIV-1 transmission from breast-feeding. Such a vaccine would need to induce protective immunity at mucosal surfaces in neonates as soon as possible after birth. Recombinant adenovirus (rAd) vectors have been s...

journal_title：Journal of virology

authors： Liu J,Li H,Iampietro MJ,Barouch DH

更新日期：2012-08-01 00:00:00

abstract：:Domains required late in the virus budding process (L domains) have been identified in the Gag proteins of a number of retroviruses. Here we show that the human T-cell leukemia virus type 1 candidate L domain motif PPPY is indeed required for virus production. Strikingly, however, mutation of this motif arrested virus...

journal_title：Journal of virology

authors： Le Blanc I,Prévost MC,Dokhélar MC,Rosenberg AR

更新日期：2002-10-01 00:00:00

abstract：:Efforts to develop adenovirus vectors suitable for genetic interventions in humans have identified three major limitations of the most frequently used vector prototype, human adenovirus serotype 5 (Ad5). These limitations--widespread preexisting anti-Ad5 immunity in humans, the high rate of transduction of normal nont...

journal_title：Journal of virology

authors： Belousova N,Mikheeva G,Xiong C,Soghomonian S,Young D,Le Roux L,Naff K,Bidaut L,Wei W,Li C,Gelovani J,Krasnykh V

更新日期：2010-10-01 00:00:00

abstract：:We have identified a spliced transcript that contains sequences from the HCMV UL29 and UL28 open reading frames. It contains amino-terminal UL29 sequences followed by UL28 sequences, and it includes a poly(A) signal derived from the 3'-untranslated region following the UL26 open reading frame. UL29/28 RNA is expressed...

journal_title：Journal of virology

authors： Mitchell DP,Savaryn JP,Moorman NJ,Shenk T,Terhune SS

更新日期：2009-10-01 00:00:00

abstract：:The barley stripe mosaic virus (BSMV) beta(b) gene product is the major viral nonstructural protein synthesized during early stages of the infection cycle and is required for systemic movement of the virus. To examine the biochemical properties of beta(b), a histidine tag was engineered at the amino terminus and the p...

journal_title：Journal of virology

authors： Donald RG,Lawrence DM,Jackson AO

更新日期：1997-02-01 00:00:00

abstract：:Barley stripe mosaic virus (BSMV) encodes three movement proteins in an overlapping triple gene block (TGB), but little is known about the physical interactions of these proteins. We have characterized a ribonucleoprotein (RNP) complex consisting of the TGB1 protein and plus-sense BSMV RNAs from infected barley plants...

journal_title：Journal of virology

authors： Lim HS,Bragg JN,Ganesan U,Lawrence DM,Yu J,Isogai M,Hammond J,Jackson AO

更新日期：2008-05-01 00:00:00

abstract：:We have recently reported the isolation and molecular cloning of a human immunodeficiency virus type 1 isolate (HIV-1 DH125) that exhibits rapid replication kinetics and marked cytopathicity in both human and chimpanzee peripheral blood mononuclear cells (PBMC). To identify the viral determinants responsible for infec...

journal_title：Journal of virology

authors： Cho MW,Shibata R,Martin MA

更新日期：1996-10-01 00:00:00

abstract：:Proteolytic processing of the picornaviral polyprotein mediated by the differential action of virus-encoded proteinase(s) is pivotal to both RNA genome replication and capsid formation. Possibly to enlarge the array of viral proteins, picornaviral polyprotein processing results in intermediate and mature products whic...

journal_title：Journal of virology

authors： Probst C,Jecht M,Gauss-Müller V

更新日期：1998-10-01 00:00:00

abstract：:The objective of this investigation was to examine by electron microscopy the replicative ribonucleoprotein (RNP) structures synthesized in vesicular stomatitis virus-infected HeLa cells. Pulse-labeled in vivo products of vesicular stomatitis virus replication and transcription can be separated by centrifugation in Re...

journal_title：Journal of virology

authors： Naeve CW,Summers DF

更新日期：1980-06-01 00:00:00

abstract：:Type 1 human immunodeficiency viruses encoding mutated nef reading frames are 10- to 30-fold less infectious than are isogenic viruses in which the nef gene is intact. This defect in infectivity causes nef-negative viruses to grow at an attenuated rate in vitro. To investigate the mechanism of Nef-mediated enhancement...

journal_title：Journal of virology

authors： Pandori MW,Fitch NJ,Craig HM,Richman DD,Spina CA,Guatelli JC

更新日期：1996-07-01 00:00:00

abstract：:It is well established that chronic viral infections can cause immune suppression, resulting in increased susceptibility to other infectious diseases. However, the effects of chronic viral infection on T-cell responses and vaccination against highly pathogenic viruses are not well understood. We have recently shown th...

journal_title：Journal of virology

authors： Alves-Peixoto P,Férez M,Knudson CJ,Melo-Silva CR,Stotesbury C,Wong EB,Correia-Neves M,Sigal LJ

更新日期：2020-02-14 00:00:00

abstract：:In cytomegalovirus-infected cells, the rate of protein synthesis was detected as two peaks. One occurred during the early phase of infection, 0 to 36 h postinfection, and the other occurred during the late phase, after the initiation of viral DNA synthesis. Double-isotopic-label difference analysis demonstrated that h...

journal_title：Journal of virology

authors： Stinski MF

更新日期：1977-09-01 00:00:00

abstract：:Herpes simplex virus (HSV) fusion with cells requires the gD, gB, and gH/gL glycoprotein quartet. gD serves as a receptor binding glycoprotein. gB and gH/gL execute fusion in an as-yet-unclear manner. To better understand the role of gH/gL in HSV entry, we produced a soluble version of gH/gL carrying a One-STrEP tag (...

journal_title：Journal of virology

authors： Gianni T,Cerretani A,Dubois R,Salvioli S,Blystone SS,Rey F,Campadelli-Fiume G

更新日期：2010-04-01 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) latently infects KS tumors, primary effusion lymphomas (PELs), and PEL cell lines. K12 (T0.7) is the most abundant transcript expressed in latent KSHV infection. We characterize here the K12 transcript from a PEL tumor prior to passage in cell culture. The PEL tumor KSHV ...

journal_title：Journal of virology

authors： Li H,Komatsu T,Dezube BJ,Kaye KM

更新日期：2002-12-01 00:00:00

abstract：:Geminiviruses are single-stranded DNA (ssDNA) viruses that infect a wide range of plants. To promote viral replication, geminiviruses manipulate the host cell cycle. The viral protein Rep is essential to reprogram the cell cycle and then initiate viral DNA replication by interacting with a plethora of nuclear host fac...

journal_title：Journal of virology

authors： Maio F,Arroyo-Mateos M,Bobay BG,Bejarano ER,Prins M,van den Burg HA

更新日期：2019-05-01 00:00:00

abstract：:Chromatography of RNA polymerase purified from vaccinia virions and from vaccinia virus-infected HeLa cells resulted in the separation of populations active for early and late transcription. An RNA polymerase population immunodepleted for the vaccinia virus H4 gene peptide could support late transcription. ...

journal_title：Journal of virology

authors： Wright CF,Coroneos AM

更新日期：1995-04-01 00:00:00

abstract：:Glycoprotein B (gB) is the most highly conserved of the envelope glycoproteins of human herpesviruses. The gB protein of human cytomegalovirus (CMV) serves multiple roles in the life cycle of the virus. To investigate structural properties of gB that give rise to its function, we sought to determine the disulfide bond...

journal_title：Journal of virology

authors： Lopper M,Compton T

更新日期：2002-06-01 00:00:00

abstract：:CM2 is the second membrane protein of the influenza C virus and has been demonstrated to play a role in the uncoating and genome packaging processes in influenza C virus replication. Although the effects of N-linked glycosylation, disulfide-linked oligomerization, and palmitoylation of CM2 on virus replication have be...

journal_title：Journal of virology

authors： Goto T,Shimotai Y,Matsuzaki Y,Muraki Y,Sho R,Sugawara K,Hongo S

更新日期：2017-10-27 00:00:00

abstract：:Cytomegalovirus (CMV) retinitis is an important ocular complication in human immunodeficiency virus-infected individuals and the leading cause of blindness in those not undergoing highly active antiretroviral therapy. Murine CMV (MCMV) infection of mice has been shown to be a useful small-animal model for the study of...

journal_title：Journal of virology

authors： Kercher L,Mitchell BM

更新日期：2002-09-01 00:00:00

abstract：:PEPSCAN analysis has been used to characterize the immunogenic regions of the capsid protein (CP) in virions of plum pox potyvirus (PPV). In addition to the well-known highly immunogenic N- and C-terminal domains of CP, regions within the core domain of the protein have also shown high immunogenicity. Moreover, the N ...

journal_title：Journal of virology

authors： Fernández-Fernández MR,Martínez-Torrecuadrada JL,Roncal F,Domínguez E,García JA

更新日期：2002-12-01 00:00:00

abstract：:The E7 oncoprotein of human papillomavirus 16 (HPV16) transforms basal and suprabasal cervical epithelial cells and is a tumor-specific antigen in cervical carcinoma, to which immunotherapeutic strategies aimed at cytotoxic T-lymphocyte (CTL) induction are currently directed. By quantifying major histocompatibility co...

journal_title：Journal of virology

authors： Tindle RW,Herd K,Doan T,Bryson G,Leggatt GR,Lambert P,Frazer IH,Street M

更新日期：2001-07-01 00:00:00