Abstract:
:Efforts to develop adenovirus vectors suitable for genetic interventions in humans have identified three major limitations of the most frequently used vector prototype, human adenovirus serotype 5 (Ad5). These limitations--widespread preexisting anti-Ad5 immunity in humans, the high rate of transduction of normal nontarget tissues, and the lack of target-specific gene delivery--justify the exploration of other Ad serotypes as vector prototypes. In this paper, we describe the development of an alternative vector platform using simian Ad serotype 24 (sAd24). We found that sAd24 virions formed unstable complexes with blood coagulation factor X and, because of that, transduced the liver and other organs at low levels when administered intravenously. The overall pattern of biodistribution of sAd24 particles was similar, however, to that of Ad5, and the intravenously injected sAd24 was cleared by Kupffer cells, leading to their depletion. We modified the virus's fiber protein to design a Her2-specific derivative of sAd24 capable of infecting target human tumor cells in vitro. In the presence of neutralizing anti-Ad5 antibodies, Her2-mediated infection with targeted sAd24 compared favorably to that with the Ad5-derived vector. When used to target Her2-expressing tumors in animals, this fiber-modified vector achieved a higher level of gene transfer to metastasis-containing murine lungs than to tumor-free lungs. In aggregate, these studies provide important insights into sAd24 biology, identify its advantages and limitations as a vector prototype, and are thus essential for further development of an sAd24-based gene delivery platform.
journal_name
J Viroljournal_title
Journal of virologyauthors
Belousova N,Mikheeva G,Xiong C,Soghomonian S,Young D,Le Roux L,Naff K,Bidaut L,Wei W,Li C,Gelovani J,Krasnykh Vdoi
10.1128/JVI.02425-09subject
Has Abstractpub_date
2010-10-01 00:00:00pages
10087-101issue
19eissn
0022-538Xissn
1098-5514pii
JVI.02425-09journal_volume
84pub_type
杂志文章abstract::Broad, multispecific CD4(+) and CD8(+) T-cell responses to the hepatitis C virus (HCV), as well as virus-cross-neutralizing antibodies, are associated with recovery from acute infection and may also be associated in chronic HCV patients with a favorable response to antiviral treatment. In order to recapitulate all of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02743-07
更新日期:2008-08-01 00:00:00
abstract::Interferon-induced transmembrane protein 3 (IFITM3) is a cellular factor that reduces HIV-1 infectivity by an incompletely understood mechanism. We show here that viruses differing only in the envelope glycoprotein (Env) expressed on their surface have different sensitivities to IFITM3. Measurements of the sensitivity...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01994-20
更新日期:2020-12-09 00:00:00
abstract::During the course of experiments designed to isolate deletion mutants of herpes simplex virus type 1 in the gene encoding the major DNA-binding protein, ICP8, a mutant, d61, that grew efficiently in ICP8-expressing Vero cells but not in normal Vero cells was isolated (P. K. Orberg and P. A. Schaffer, J. Virol. 61:1136...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.11.3528-3535.1987
更新日期:1987-11-01 00:00:00
abstract::The time course of murine leukemia virus production after chemical induction was determined in hamster-mouse somatic cell hybrids containing the xenotropic murine leukemia virus induction locus Bxv-1 or the ecotropic locus Akv-2. By using these hybrids, induction could be studied in the absence of secondary virus spre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.876-879.1984
更新日期:1984-09-01 00:00:00
abstract::Hepatitis E virus (HEV) replication is not well understood, mainly because the virus does not infect cultured cells efficiently. However, Huh-7 cells transfected with full-length genomes produce open reading frame 2 protein, indicative of genome replication (6). To investigate the role of 3'-terminal sequences in RNA ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1017-1026.2005
更新日期:2005-01-01 00:00:00
abstract::A herpes simplex virus type 1 (HSV-1) strain, B3, was found to have a short duplication on the left junction between the unique sequence (US) and the inverted repeat sequence (RS) in the S component of the genome DNA. A short region of RS contiguous to the left US-RS junction was duplicated in B3. Based on the nucleot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.1877-1883.1989
更新日期:1989-05-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) lytic infection increases the expression of viral and human interleukin-6 (vIL-6 and hIL-6, respectively), an important factor for cell growth and pathogenesis. Here, we report genome-wide analysis of viral RNA targets of KSHV ORF57 by a novel UV-cross-linking and immunop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02144-10
更新日期:2011-03-01 00:00:00
abstract::Foxp3(+) CD4(+) regulatory T cells (Tregs) represent a highly suppressive T cell subset with well-characterized immunosuppressive effects during immune homeostasis and chronic infections, although the role of these cells in acute viral infections is poorly understood. The present study sought to examine the induction ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05685-11
更新日期:2012-03-01 00:00:00
abstract::Human trophoblast cells were permissively infected by human cytomegalovirus. The kinetics of viral immediate-early, early, and late gene expression was clearly delayed compared to that in fibroblasts. Productive infection was unequivocally proven by the detection of virion particles, infectious virus in trophoblast cu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7598-7602.1998
更新日期:1998-09-01 00:00:00
abstract::Persistent human immunodeficiency virus (HIV) infection of human monocytes and macrophages increases I kappa B alpha degradation, resulting in the activation of NF-kappa B, a key transcription factor in the regulation of the HIV long terminal repeat. The signal transduction pathways leading to NF-kappa B activation in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2332-2338.1996
更新日期:1996-04-01 00:00:00
abstract:UNLABELLED:Viruses that spread systemically from a peripheral site of infection cause morbidity and mortality in the human population. Innate myeloid cells, including monocytes, macrophages, monocyte-derived dendritic cells (mo-DC), and dendritic cells (DC), respond early during viral infection to control viral replica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01024-15
更新日期:2015-10-01 00:00:00
abstract::Minimal transformants of rat F111 fibroblasts were established after infection with the large T antigen (large T)-encoding retroviral expression vector pZIPTEX (M. Brown, M. McCormack, K. Zinn, M. Farrell, I. Bikel, and D. Livingston, J. Virol. 60:290-293, 1986). Coexpression of small t antigen (small t) in these cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1555-1563.1993
更新日期:1993-03-01 00:00:00
abstract::Hypoxia-inducible factor 1 (HIF-1) is a ubiquitously expressed transcriptional regulator involved in induction of numerous genes associated with angiogenesis and tumor growth. Kaposi's sarcoma, associated with increased angiogenesis, is a highly vascularized, endothelial cell-derived tumor. Previously, we have shown t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00611-07
更新日期:2007-10-01 00:00:00
abstract::T cells are central players in the immune response to infectious disease, with the specificity of their responses controlled by the T-cell receptor (TCR)/CD3 complex on the cell surface. Impairment of TCR/CD3-directed CD4(+) T-cell immune responses is frequently observed in individuals infected with human immunodefici...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.5207-5213.1999
更新日期:1999-06-01 00:00:00
abstract::Kaposi sarcoma-associated herpesvirus (KSHV) is necessary but not sufficient for primary effusion lymphoma (PEL) development. Alterations in cellular signaling pathways are also a characteristic of PEL. Other B cell lymphomas have acquired an oncogenic mutation in the myeloid differentiation primary response 88 (MYD88...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02123-19
更新日期:2020-05-04 00:00:00
abstract::Nef, an accessory protein of human and simian immunodeficiency viruses, is a critical determinant of pathogenesis that promotes the progression from infection to AIDS. The pathogenic effects of Nef are in large part dependent on its ability to downregulate the macrophage and T-cell coreceptor, CD4. It has been propose...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02725-06
更新日期:2007-04-01 00:00:00
abstract::Different tombusviruses were able to support the replication of either homologous or heterologous defective interfering (DI) RNAs, and those infected plants usually developed typical attenuated symptoms. However, in some helper virus-DI RNA combinations the inoculated plants were necrotized, although they contained a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.6251-6256.1998
更新日期:1998-07-01 00:00:00
abstract:UNLABELLED:Despite the nutritional and health benefits of breast milk, breast milk can serve as a vector for mother-to-child HIV transmission. Most HIV-infected infants acquire HIV through breastfeeding. Paradoxically, most infants breastfed by HIV-positive women do not become infected. This is potentially attributed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01702-15
更新日期:2015-11-01 00:00:00
abstract::Recent studies have established the presence of human T-cell lymphoma/leukemia virus type I (HTLV-I) in Melanesia. An HTLV-I strain, PNG-1, has now been isolated from a healthy member of the Hagahai, a remote, recently contacted group in Papua New Guinea. To further characterize PNG-1, we employed polymerase chain rea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2556-2563.1992
更新日期:1992-04-01 00:00:00
abstract::The human cytomegalovirus UL47 open reading frame encodes a 110-kDa protein that is a component of the virion tegument. We have constructed a cytomegalovirus mutant, ADsubUL47, in which the central portion of the UL47 open reading frame has been replaced by two marker genes. The mutant replicated to titers 100-fold lo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1043-1050.2002
更新日期:2002-02-01 00:00:00
abstract::Massive infection of memory CD4 T cells is a hallmark of early simian immunodeficiency virus (SIV) infection, with viral infection peaking at day 10 postinfection (p.i.), when a majority of memory CD4 T cells in mucosal and peripheral tissues are infected. It is not clear if mononuclear cells from the monocyte and mac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06372-11
更新日期:2012-01-01 00:00:00
abstract::Mumps is a highly contagious human disease, characterized by lateral or bilateral nonsuppurative swelling of the parotid glands and neurological complications that can result in aseptic meningitis or encephalitis. A mumps vaccination program implemented since the 1960s reduced mumps incidence by more than 99% and kept...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02778-13
更新日期:2014-03-01 00:00:00
abstract::The full repertoire of hepatitis B virus (HBV) peptides that bind to the common HLA class I molecules found in areas with a high prevalence of chronic HBV infection has not been determined. This information may be useful for designing immunotherapies for chronic hepatitis B. We identified amino acid residues under pos...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01471-09
更新日期:2010-01-01 00:00:00
abstract::Autoradiographic analyses of deoxyribonucleic acid (DNA) synthesis in randomly growing KB cell cultures infected with equine abortion virus (EAV) suggested that viral DNA synthesis was initiated only at times that coincided with the entry of noninfected control cells into the S phase of the cell cycle. Synchronized cu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.736-748.1971
更新日期:1971-06-01 00:00:00
abstract::The majority of double-stranded DNA (dsDNA) viruses infecting eukaryotic organisms use host- or virus-expressed histones or protamine-like proteins to condense their genomes. In contrast, members of the Baculoviridae family use a protamine-like protein named P6.9. The dephosphorylated form of P6.9 binds to DNA in a no...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00072-10
更新日期:2010-09-01 00:00:00
abstract::The synthesis of Sendai virus RNA species was examined after UV irradiation of cells late in infection. Compared with the inactivation of 50S genomic RNA synthesis, the synthesis of the group of mRNA species that sediments at 18S was inactivated at an average rate consistent with a process of sequential transcription ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.863-871.1977
更新日期:1977-03-01 00:00:00
abstract::We isolated, purified, and characterized the hemagglutinin-neuraminidase (HN) of human parainfluenza virus type 1, with the ultimate goal of producing crystals suitable for three-dimensional X-ray structure analysis. Pronase was used to cleave the globular head of the HN molecule directly from virus particles, forming...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7597-7600.1992
更新日期:1992-12-01 00:00:00
abstract::Most poxviruses express multiple proteins containing ankyrin (ANK) repeats accounting for a large superfamily of related but unique determinants of poxviral tropism. Recently, select members of this novel family of poxvirus proteins have drawn considerable attention for their potential roles in modulating intracellula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00963-09
更新日期:2009-12-01 00:00:00
abstract::We evaluated the ability of human coronaviruses to infect primary cultures of human neural cells. Double immunofluorescence with antibodies to virus and cell markers showed infection of fetal astrocytes and of adult microglia and astrocytes by strain OC43. RNA amplification revealed infection of fetal astrocytes, adul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.800-806.1997
更新日期:1997-01-01 00:00:00
abstract:UNLABELLED:Japanese encephalitis virus (JEV) membrane (M) protein plays important structural roles in the processes of fusion and maturation of progeny virus during cellular infection. The M protein is anchored in the viral membrane, and its ectodomain is composed of a flexible N-terminal loop and a perimembrane helix....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01176-15
更新日期:2015-12-09 00:00:00