Abstract:
:PEPSCAN analysis has been used to characterize the immunogenic regions of the capsid protein (CP) in virions of plum pox potyvirus (PPV). In addition to the well-known highly immunogenic N- and C-terminal domains of CP, regions within the core domain of the protein have also shown high immunogenicity. Moreover, the N terminus of CP is not homogeneously immunogenic, alternatively showing regions frequently recognized by antibodies and others that are not recognized at all. These results have helped us to design efficient antigen presentation vectors based on PPV. As predicted by PEPSCAN analysis, a small displacement of the insertion site in a previously constructed vector, PPV-gamma, turned the derived chimeras into efficient immunogens. Vectors expressing foreign peptides at different positions within a highly immunogenic region (amino acids 43 to 52) in the N-terminal domain of CP were the most effective at inducing specific antibody responses against the foreign sequence.
journal_name
J Viroljournal_title
Journal of virologyauthors
Fernández-Fernández MR,Martínez-Torrecuadrada JL,Roncal F,Domínguez E,García JAdoi
10.1128/jvi.76.24.12646-12653.2002subject
Has Abstractpub_date
2002-12-01 00:00:00pages
12646-53issue
24eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
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pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of virology
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更新日期:1982-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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pub_type: 杂志文章,收录出版
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更新日期:2004-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-02-01 00:00:00