Abstract:
:Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detail. The initiation of RCR of the bipartite genome of a geminivirus, Indian mung bean yellow mosaic virus (IMYMV), is mainly controlled by viral protein Rep (or AL1 or AC1). The role of host PCNA in RCR of IMYMV was revealed by studying the physical and functional interactions between recombinant PCNA and recombinant IMYMV Rep. Pea nuclear PCNA as well as recombinant pea PCNA showed binding to recombinant Rep in experiments involving both affinity chromatography and yeast two-hybrid approaches. The contacting amino acid residues of PCNA seemed to be present throughout a wide region of the trimeric protein, while those of Rep appeared to be localized only in the middle part of the protein. The site-specific nicking-closing activity and the ATPase function of IMYMV Rep were impaired by PCNA. These observations lead to interesting speculations about the control of viral RCR and dynamic profiles of protein-protein interactions at the RCR origin of the geminiviruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bagewadi B,Chen S,Lal SK,Choudhury NR,Mukherjee SKdoi
10.1128/JVI.78.21.11890-11903.2004subject
Has Abstractpub_date
2004-11-01 00:00:00pages
11890-903issue
21eissn
0022-538Xissn
1098-5514pii
78/21/11890journal_volume
78pub_type
杂志文章abstract::Adeno-associated virus (AAV) is a human parvovirus currently being developed as a vector for gene therapy applications. Because the gene transfer vector commonly retains only the AAV terminal repeats, propagation of recombinant AAV (rAAV) requires that the viral replication (Rep) and capsid (Cap) proteins be supplied ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1897-1905.1997
更新日期:1997-03-01 00:00:00
abstract::Gene transfer to differentiated airway epithelia with existing viral vectors is very inefficient when they are applied to the apical surface. This largely reflects the polarized distribution of receptors on the basolateral surface. To identify new receptor-ligand interactions that might be used to redirect vectors to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9234-9239.2000
更新日期:2000-10-01 00:00:00
abstract::A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus 569 was isolated from soil. All auxotrophic mutants tested, those requiring tryptophan, histidine, leucine, isoleucine, methionine, or phenylalanine, were transduced to prototrophy. The phage was extremely unstable when stored at 2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.7.657-662.1968
更新日期:1968-07-01 00:00:00
abstract::Expression of the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax is correlated with cellular transformation contributing to the development of adult T-cell leukemia. Tax has been shown to modulate the activities of several cellular promoters. Existing evidence suggests that Tax need not directly bind to D...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.5.2161-2173.2001
更新日期:2001-03-01 00:00:00
abstract::The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) produces multiple mRNAs through differential splicing and polyadenylation. Reverse transcriptase PCR was used to characterize transcripts from exons 1, 2, 3, and 4 (immediate-early 1 [IE1]). The expected IE72 and IE19 mRNAs were detected, as well as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.8191-8200.2004
更新日期:2004-08-01 00:00:00
abstract:UNLABELLED:Human T-cell lymphotropic virus type 1 (HTLV-1) and HTLV-2 encode auxiliary proteins that play important roles in viral replication, viral latency, and immune escape. The presence of auxiliary protein-encoding open reading frames (ORFs) in HTLV-3, the latest HTLV to be discovered, is unknown. Simian T-cell l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02150-14
更新日期:2015-01-15 00:00:00
abstract::Murine monoclonal antibodies directed against the structural proteins p17 and p24 of human immunodeficiency virus type 1 were investigated in an epitope mapping system. Overlapping peptides consisting of 15 amino acids of the p17 and p24 protein, respectively, were used as competitors in an enzyme-linked immunosorbent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3525-3528.1989
更新日期:1989-08-01 00:00:00
abstract::A virus (M-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus RD-114. Immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between M-7 and RD-114. Anti-RD-114 DNA polymerase IgG inhibits M-7 polymerase by 57% compared to 97% for RD-114. M-7 vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.133-138.1974
更新日期:1974-07-01 00:00:00
abstract::3-O-(3',3'-Dimethysuccinyl) betulinic acid (PA-457) has been shown to potently inhibit human immunodeficiency virus (HIV) replication in culture. In contrast to inhibitors that act upon the viral proteinase, PA-457 appears to block only the final maturational cleavage of p25CA-p2 to p24CA. However, attempts to replica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02743-05
更新日期:2006-06-01 00:00:00
abstract::The relative contribution of measles virus hemagglutinin (H)- or fusion protein (F)-specific antibodies to virus neutralization (VN) has not been demonstrated. We have depleted these specific antibodies from sera collected from young adults, who had been vaccinated during childhood, by prolonged incubation with viable...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11547-11551.2005
更新日期:2005-09-01 00:00:00
abstract::We have proposed a general algorithm for identification of potential immunoprotective domains (cassettes) on the envelope E2 polypeptide of alphaviruses (H. Grosfeld, B. Velan, M. Leitner, S. Cohen, S. Lustig, B.E. Lachmi, and A. Shafferman, J. Virol. 63:3416-3422, 1989). To assess the generality of our approach, we c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.1084-1090.1992
更新日期:1992-02-01 00:00:00
abstract::HIV circumvents HLA class I-restricted CD8+ T-cell responses through selection of escape mutations that leave characteristic mutational "footprints," also known as HLA-associated polymorphisms (HAPs), on HIV sequences at the population level. While many HLA footprints are universal across HIV subtypes and human popula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01128-17
更新日期:2018-01-02 00:00:00
abstract::The HIV-1 Vpr protein participates in the early steps of the virus life cycle by influencing the accuracy of reverse transcription. This role of Vpr was related to the recruitment of the nuclear form of the uracil DNA glycosylase (UNG2) enzyme into virus particles, but several conflicting findings have been reported r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05163-11
更新日期:2012-03-01 00:00:00
abstract::GLUT1 has recently been suggested to be a binding receptor for human T-cell leukemia virus type 1 (HTLV-1). We used a novel, short-term assay to define the role of GLUT1 in cell-to-cell transmission. Although increasing cell surface levels of GLUT1 enhanced HTLV-I transfer, efficient virus spread correlated largely wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01522-06
更新日期:2007-02-01 00:00:00
abstract::Satellite RNAs are the smallest infectious agents whose replication is thought to be completely dependent on their helper virus (HV). Here we report that, when expressed autonomously in the absence of HV, a variant of satellite RNA (satRNA) associated with Cucumber mosaic virus strain Q (Q-satRNA) has a propensity to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00018-12
更新日期:2012-05-01 00:00:00
abstract:UNLABELLED:Equine influenza viruses (EIV) are responsible for rapidly spreading outbreaks of respiratory disease in horses. Although natural infections of humans with EIV have not been reported, experimental inoculation of humans with these viruses can lead to a productive infection and elicit a neutralizing antibody r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02449-14
更新日期:2015-02-01 00:00:00
abstract::The Chinese hamster cell lines E36 and CHOK1 dramatically differ in susceptibility to amphotropic murine leukemia virus (A-MuLV) and gibbon ape leukemia virus (GALV); E36 cells are highly susceptible to both viruses, CHOK1 cells are not. We have previously shown that GALV can infect E36 cells by using both its own rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2916-2920.1999
更新日期:1999-04-01 00:00:00
abstract::Latency-associated nuclear antigen 1 (LANA1) of Kaposi's sarcoma-associated herpesvirus (KSHV) is implicated in the persistence of the viral genome during latent infection. It has been suggested that LANA1 tethers the viral genome to the host chromosome and also participates actively in DNA replication from the termin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7248-7256.2004
更新日期:2004-07-01 00:00:00
abstract::We tested chemokine receptor subset usage by diverse, well-characterized primary viruses isolated from peripheral blood by monitoring viral replication with CCR1, CCR2b, CCR3, CCR5, and CXCR4 U87MG.CD4 transformed cell lines and STRL33/BONZO/TYMSTR and GPR15/BOB HOS.CD4 transformed cell lines. Primary viruses were iso...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9307-9312.1998
更新日期:1998-11-01 00:00:00
abstract::The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) initiates apoptosis in diverse insects through events triggered by virus DNA (vDNA) replication. To define the proapoptotic pathway and its role in antivirus defense, we investigated the link between the host's DNA damage response (DDR) a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02246-12
更新日期:2012-12-01 00:00:00
abstract::Paired immunoglobulin (Ig)-like type 2 receptor alpha (PILRalpha) and PILRbeta are paired receptors that are highly homologous to each other. When engaged by ligand, PILRalpha is inhibitory whereas PILRbeta is activating. PILRalpha is a newly identified herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) receptor ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01039-10
更新日期:2010-09-01 00:00:00
abstract::Nonstructural protein 5A (NS5A) is a membrane-associated essential component of the hepatitis C virus (HCV) replication complex. An N-terminal amphipathic alpha helix mediates in-plane membrane association of HCV NS5A and at the same time is likely involved in specific protein-protein interactions required for the ass...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01279-06
更新日期:2007-03-01 00:00:00
abstract::RNA interference (RNAi) is a conserved antiviral immune defense in eukaryotes, and numerous viruses have been found to encode viral suppressors of RNAi (VSRs) to counteract antiviral RNAi. Alphaviruses are a large group of positive-stranded RNA viruses that maintain their transmission and life cycles in both mosquitoe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01233-19
更新日期:2020-01-17 00:00:00
abstract::The present study was designed to investigate the endocytic pathway involved in canine parvovirus (CPV) infection. Reduced temperature (18 degrees C) or the microtubule-depolymerizing drug nocodazole was found to inhibit productive infection of canine A72 cells by CPV and caused CPV to be retained in cytoplasmic vesic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.802-806.1998
更新日期:1998-01-01 00:00:00
abstract::CD8(+) cytotoxic T lymphocytes (CTL) play a key role in the control of many virus infections, and the need for vaccines to elicit strong CD8(+) T-cell responses in order to provide optimal protection in such infections is increasingly apparent. However, the mechanisms involved in the induction and maintenance of CD8(+...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7440-7449.1998
更新日期:1998-09-01 00:00:00
abstract::Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome consisting of six negative-stranded RNA segments. To rescue a recombinant THOV, the viral structural proteins were produced from expression plasmids by means of a vaccinia virus expressing the T7 RNA polymerase. Genomic virus RNAs (vRNAs) were gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9282-9286.2001
更新日期:2001-10-01 00:00:00
abstract::The effect of NS1 protein on the efficiency of influenza virus mRNA translation was evaluated by determining the accumulation of nucleoprotein (NP) or M1 mRNAs in the cytoplasm of cells expressing either of these genes alone or in combination with the NS1 gene, as well as the total cell accumulation of NP or M1 protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2427-2433.1995
更新日期:1995-04-01 00:00:00
abstract::We report here the isolation by molecular cloning and the analysis by heteroduplex and restriction enzyme mapping of seven distinct DNA fragments containing hepatitis B virus (HBV) sequences from genomic DNA of the PLC/PRF/5 human liver carcinoma cell line (the Alexander cell). No intact full-length HBV genomes were p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.776-787.1984
更新日期:1984-09-01 00:00:00
abstract::Polypeptides synthesized in Newcastle disease virus (NDV)-infected CHO cells in the absence of glycosylation were characterized. Incorporation of either [3H]mannose of [3H]glucosamine into NDV polypeptides was inhibited to greater than 99% by the antibiotic tunicamycin. Under these conditions, infected cells synthesiz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.1.171-180.1980
更新日期:1980-10-01 00:00:00
abstract::Long interspersed nuclear element 1 (LINE-1; L1) retrotransposons are the most common retroelements in mammalian genomes. Unlike individual families of endogenous retroviruses (ERVs), they have remained active throughout the mammalian radiation and are responsible for most of the retroelement movement and much genome ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05180-11
更新日期:2011-12-01 00:00:00