Abstract:
:Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome consisting of six negative-stranded RNA segments. To rescue a recombinant THOV, the viral structural proteins were produced from expression plasmids by means of a vaccinia virus expressing the T7 RNA polymerase. Genomic virus RNAs (vRNAs) were generated from plasmids under the control of the RNA polymerase I promoter. Using this system, we could efficiently recover recombinant THOV following transfection of 12 plasmids into 293T cells. To verify the recombinant nature of the rescued virus, specific genetic tags were introduced into two vRNA segments. The availability of this efficient reverse genetics system will allow us to address hitherto-unanswered questions regarding the biology of THOV by manipulating viral genes in the context of infectious virus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wagner E,Engelhardt OG,Gruber S,Haller O,Kochs Gdoi
10.1128/JVI.75.19.9282-9286.2001subject
Has Abstractpub_date
2001-10-01 00:00:00pages
9282-6issue
19eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
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