Inhibition of viral RNA methylation in herpes simplex virus type 1-infected cells by 5' S-isobutyl-adenosine.


:5' S-isobutyl-adenosine (SIBA), a structural analogue of S-adenosylhomocysteine, reversibly blocks the multiplication of herpes simplex type 1 virus. In the presence of SIBA, viral protein synthesis is inhibited. After removing SIBA the synthesis of proteins starts rapidly again. The new polypeptides are mainly alpha proteins (Honess and Roizman, J. Virol. 14:8-19, 1974,), normally the first to be synthesized after infection. The rapid synthesis of proteins after release of inhibition seems to be directed by mRNA formed in the presence of SIBA as indicated by experiments using actinomycin D but which was undermethylated as shown by analysis of methyl groups on RNA. SIBA inhibits the methylation of mRNA and especially that of the 5' cap. Capping of mRNA thus seems to be essential for efficient translation. The analogue affected various methylations to different extents.


J Virol


Journal of virology


Jacquemont B,Huppert J




Has Abstract


1977-04-01 00:00:00












  • Molecular characterization of proteolytic processing of the Gag proteins of human spumavirus.

    abstract::Spumaviruses, or foamy viruses, express Gag proteins that are incompletely processed by the viral protease in cell cultures. To delineate the proteolytic cleavage sites between potential Gag subdomains, recombinant human spumaretrovirus (HSRV) Gag proteins of different lengths were expressed, purified by affinity chro...

    journal_title:Journal of virology

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    authors: Pfrepper KI,Löchelt M,Rackwitz HR,Schnölzer M,Heid H,Flügel RM

    更新日期:1999-09-01 00:00:00

  • The C-terminal proline-rich tail of human immunodeficiency virus type 2 Vpx is necessary for nuclear localization of the viral preintegration complex in nondividing cells.

    abstract::Human immunodeficiency virus type 2 (HIV-2), like other lentiviruses, is capable of infecting nondividing T cells and macrophages. The present work shows that in HIV-2-infected cells, Vpx is necessary for efficient nuclear import of the preintegration complex. In agreement with this finding, the subcellular localizati...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pancio HA,Vander Heyden N,Ratner L

    更新日期:2000-07-01 00:00:00

  • Structural definition of an antibody-dependent cellular cytotoxicity response implicated in reduced risk for HIV-1 infection.

    abstract:UNLABELLED:The RV144 vaccine trial implicated epitopes in the C1 region of gp120 (A32-like epitopes) as targets of potentially protective antibody-dependent cellular cytotoxicity (ADCC) responses. A32-like epitopes are highly immunogenic, as infected or vaccinated individuals frequently produce antibodies specific for ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Acharya P,Tolbert WD,Gohain N,Wu X,Yu L,Liu T,Huang W,Huang CC,Kwon YD,Louder RK,Luongo TS,McLellan JS,Pancera M,Yang Y,Zhang B,Flinko R,Foulke JS Jr,Sajadi MM,Kamin-Lewis R,Robinson JE,Martin L,Kwong PD,Guan

    更新日期:2014-11-01 00:00:00

  • Role of naturally occurring basic amino acid substitutions in the human immunodeficiency virus type 1 subtype E envelope V3 loop on viral coreceptor usage and cell tropism.

    abstract::To assess the role of naturally occurring basic amino acid substitutions in the V3 loop of human immunodeficiency virus type 1 (HIV-1) subtype E on viral coreceptor usage and cell tropism, we have constructed a panel of chimeric viruses with mutant V3 loops of HIV-1 subtype E in the genetic background of HIV-1LAI. The...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kato K,Sato H,Takebe Y

    更新日期:1999-07-01 00:00:00

  • L protein requirement for in vitro RNA synthesis by vesicular stomatitis virus.

    abstract::The endogenous transcriptase present in purified vesicular stomatitis (VS) virions was solubilized with a Triton X-100 high-salt solution. The polymerase activity was purified on glycerol gradients and by phosphocellulose column chromatography; the viral proteins present in the active enzyme fractions were identified ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Emerson SU,Wagner RR

    更新日期:1973-12-01 00:00:00

  • Increased immunogenicity of human immunodeficiency virus gp120 engineered to express Galalpha1-3Galbeta1-4GlcNAc-R epitopes.

    abstract::The glycan shield comprised of multiple carbohydrate chains on the human immunodeficiency virus (HIV) envelope glycoprotein gp120 helps the virus to evade neutralizing antibodies. The present study describes a novel method for increasing immunogenicity of gp120 vaccine by enzymatic replacement of sialic acid on these ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Abdel-Motal U,Wang S,Lu S,Wigglesworth K,Galili U

    更新日期:2006-07-01 00:00:00

  • Differentiation-specific transcriptional regulation of the hepatitis B virus large surface antigen gene in human hepatoma cell lines.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Raney AK,Milich DR,Easton AJ,McLachlan A

    更新日期:1990-05-01 00:00:00

  • Dissecting the Role of DDX21 in Regulating Human Cytomegalovirus Replication.

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  • Influenza Virus and Chromatin: Role of the CHD1 Chromatin Remodeler in the Virus Life Cycle.

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    authors: Marcos-Villar L,Pazo A,Nieto A

    更新日期:2016-01-20 00:00:00

  • Insertion mutation of the int-1 and int-2 loci by mouse mammary tumor virus in premalignant and malignant neoplasms from the GR mouse strain.

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    更新日期:1990-04-01 00:00:00

  • Imparting temperature sensitivity and attenuation in ferrets to A/Puerto Rico/8/34 influenza virus by transferring the genetic signature for temperature sensitivity from cold-adapted A/Ann Arbor/6/60.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Jin H,Zhou H,Lu B,Kemble G

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  • Nef-induced CD4 endocytosis in human immunodeficiency virus type 1 host cells: role of p56lck kinase.

    abstract::Human immunodeficiency virus type 1 (HIV-1) Nef interferes with the endocytic machinery to modulate the cell surface expression of CD4. However, the basal trafficking of CD4 is governed by different rules in the target cells of HIV-1: whereas CD4 is rapidly internalized from the cell surface in myeloid cells, CD4 is s...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Laguette N,Brégnard C,Bouchet J,Benmerah A,Benichou S,Basmaciogullari S

    更新日期:2009-07-01 00:00:00

  • The laminin receptor is a cellular attachment receptor for classical Swine Fever virus.

    abstract:UNLABELLED:Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious, economically important viral disease in many countries. The E(rns) and E2 envelope glycoproteins are responsible for the binding to and entry into the host cell by CSFV. To date, only one cellular r...

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    pub_type: 杂志文章


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    更新日期:2015-05-01 00:00:00

  • Association of 4S ribonucleic acid with oncornavirus ribonucleic acids.

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    journal_title:Journal of virology

    pub_type: 杂志文章


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  • Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Li D,Li S,Sun Y,Dong H,Li Y,Zhao B,Guo D,Weng C,Qiu HJ

    更新日期:2013-02-01 00:00:00

  • Induction of necrotic-like cell death by tumor necrosis factor alpha and caspase inhibitors: novel mechanism for killing virus-infected cells.

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    pub_type: 杂志文章


    authors: Li M,Beg AA

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  • Formation and possible functions of alpha-putrescinylthymine in bacteriophage phi W-14 DNA: analysis of bacteriophage mutants with decreased levels of alpha-putrescinylthymine in their DNAs.

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    pub_type: 杂志文章


    authors: Miller PB,Scraba DG,Leyritz-Wills M,Maltman KL,Warren RA

    更新日期:1983-09-01 00:00:00

  • Infection of primary cultures of human neural cells by human coronaviruses 229E and OC43.

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    pub_type: 杂志文章


    authors: Bonavia A,Arbour N,Yong VW,Talbot PJ

    更新日期:1997-01-01 00:00:00

  • Four EBNA2 domains are important for EBNALP coactivation.

    abstract::EBNA2 transcriptional activation and regulated EBNALP coactivation are critical for Epstein-Barr virus-infected primary B-lymphocyte growth transformation. EBNALP coactivation requires the EBNA2 acidic activation domain (E2AD); EBNALP can bind to E2AD. EBNALP has now been found to bind less well to EBNA2 amino acids 1...

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    pub_type: 杂志文章


    authors: Peng CW,Zhao B,Kieff E

    更新日期:2004-10-01 00:00:00

  • Epstein-Barr virus infection of human gastric carcinoma cells: implication of the existence of a new virus receptor different from CD21.

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    pub_type: 杂志文章


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  • Ongoing viral replication is required for gammaherpesvirus 68-induced vascular damage.

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    pub_type: 杂志文章


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    journal_title:Journal of virology

    pub_type: 杂志文章


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  • Second-site mutations selected in transcriptional regulatory sequences compensate for engineered mutations in the vesicular stomatitis virus nucleocapsid protein.

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    journal_title:Journal of virology

    pub_type: 杂志文章


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  • Innate Sensing of Influenza A Virus Hemagglutinin Glycoproteins by the Host Endoplasmic Reticulum (ER) Stress Pathway Triggers a Potent Antiviral Response via ER-Associated Protein Degradation.

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    journal_title:Journal of virology

    pub_type: 杂志文章


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  • Interviral Recombination between Plant, Insect, and Fungal RNA Viruses: Role of the Intracellular Ca2+/Mn2+ Pump.

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    pub_type: 杂志文章


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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Platt TH,Tcherepanova IY,Schildkraut CL

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hecht TT,Summers DF

    更新日期:1972-10-01 00:00:00

  • Presentation of native epitopes in the V1/V2 and V3 regions of human immunodeficiency virus type 1 gp120 by fusion glycoproteins containing isolated gp120 domains.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kayman SC,Wu Z,Revesz K,Chen H,Kopelman R,Pinter A

    更新日期:1994-01-01 00:00:00