Abstract:
:Two mutant strains (vi12 and vi13) of herpes simplex virus that contain insertion mutations in the sequences that encode the DNA-binding domain of viral regulatory protein ICP4 were generated. Both mutations disrupted specific DNA binding and resulted in transcriptionally inactive molecules; however, the ability of the mutant proteins to form dimers was retained. The mutant proteins formed heterodimers with an ICP4 deletion mutant (X25) that is nonfunctional but retains the ability to bind to consensus sites. Significantly elevated levels of early (E or beta) and "leaky late" (beta gamma or gamma 1) gene expression were observed upon coexpression of the insertion mutant and X25 ICP4 polypeptides. While the heterodimers composed of the vi13 and X25 peptides possessed DNA-binding activity, those composed of vi12 and X25 did not, indicating that DNA binding by the heterodimers may not be required for restored activity. Despite significant levels of early gene expression and viral DNA synthesis in vi12-infected X25 cells, true late (gamma 2) mRNA was not synthesized. This indicates that the structural requirements for ICP4 induction of different classes of viral genes may be different.
journal_name
J Viroljournal_title
Journal of virologyauthors
Shepard AA,DeLuca NAdoi
10.1128/JVI.65.1.299-307.1991subject
Has Abstractpub_date
1991-01-01 00:00:00pages
299-307issue
1eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
杂志文章abstract::Deletion mutants of simian virus 40 (SV40) with lesions at the three DdeI sites near the 3' end of the early region were constructed. Mutants with deletions at 0.203 and 0.219 map units (mu) which did not change the large T antigen reading frame were viable. This extends slightly the upstream boundary for the location...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.3.487-494.1983
更新日期:1983-09-01 00:00:00
abstract::The NS3 protein of hepatitis C virus (HCV) possesses protease activity responsible for the proteolytic cleavage of the viral polyprotein at the junctions of nonstructural proteins downstream of NS3. The NS3 protein was also found to be internally cleaved. In this study, we demonstrated that internal cleavages occurred...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00348-07
更新日期:2007-08-01 00:00:00
abstract::The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2494-2501.2004
更新日期:2004-03-01 00:00:00
abstract::Seasonal influenza viruses are a major cause of human disease worldwide. Most neutralizing antibodies (Abs) elicited by influenza viruses target the head domain of the hemagglutinin (HA) protein. Anti-HA head Abs can be highly potent, but they have limited breadth since the HA head is variable. There is great interest...
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pub_type: 临床试验,杂志文章,多中心研究
doi:10.1128/JVI.02134-18
更新日期:2019-04-03 00:00:00
abstract::The retroviral Gag protein plays the central role in the assembly process and can form membrane-enclosed, virus-like particles in the absence of any other viral products. These particles are similar to authentic virions in density and size. Three small domains of the human immunodeficiency virus type 1 (HIV-1) Gag pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4667-4677.1998
更新日期:1998-06-01 00:00:00
abstract::Human papillomavirus (HPV) is a strongly conserved DNA virus, high-risk types of which can cause cervical cancer in persistent infections. The most common type found in HPV-attributable cancer is HPV16, which can be subdivided into four lineages (A to D) with different carcinogenic properties. Studies have shown HPV16...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00844-17
更新日期:2017-09-12 00:00:00
abstract:UNLABELLED:Baculovirus DNAs are synthesized and inserted into preformed capsids to form nucleocapsids at a site in the infected cell nucleus, termed the virogenic stroma. Nucleocapsid assembly ofAutographa californicamultiple nucleopolyhedrovirus (AcMNPV) requires the major capsid protein VP39 and nine minor capsid pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02885-15
更新日期:2016-03-28 00:00:00
abstract::The induction of an interferon-induced antiviral state is a powerful cellular response against viral infection that limits viral spread. Here, we show that a preexisting antiviral state inhibits the replication of influenza A viruses in human A549 cells by preventing transport of the viral genome to the nucleus and th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02220-13
更新日期:2013-12-01 00:00:00
abstract::The UL16 protein of herpes simplex virus is capsid associated and was previously identified as a binding partner of the membrane-associated UL11 tegument protein (J. S. Loomis, R. J. Courtney, and J. W. Wills, J. Virol. 77:11417-11424, 2003). In those studies, a less-prominent, approximately 65-kDa binding partner of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02015-09
更新日期:2010-03-01 00:00:00
abstract:UNLABELLED:The APOBEC3 family of DNA cytosine deaminases has important roles in innate immunity and cancer. It is unclear how DNA tumor viruses regulate these enzymes and how these interactions, in turn, impact the integrity of both the viral and cellular genomes. Polyomavirus (PyVs) are small DNA pathogens that contai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00771-16
更新日期:2016-06-24 00:00:00
abstract::Epstein-Barr virus is associated with several human malignancies including Burkitt's lymphoma, nasopharyngeal carcinoma, and Hodgkin's disease (HD). To examine the effect of Epstein-Barr virus nuclear antigen 1 (EBNA-1) in the pathogenesis of HD, we transfected the gene into the HD cell line L428. EBNA-1 expression wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1630-1636.1999
更新日期:1999-02-01 00:00:00
abstract::Inhibition of hepatitis B virus (HBV) replication and viral clearance from an infected host requires both the innate and adaptive immune responses. Expression of interferon (IFN)-inducible proteasome catalytic and regulatory subunits correlates with the IFN-alpha/beta- and IFN-gamma-mediated noncytopathic inhibition o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01779-06
更新日期:2007-01-01 00:00:00
abstract::The infected cell polypeptide 4 (ICP4) of herpes simplex virus 1 (HSV-1) is a regulator of viral transcription that is required for productive infection. Since viral genes are transcribed by cellular RNA polymerase II (RNA pol II), ICP4 must interact with components of the pol II machinery to regulate viral gene expre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00385-11
更新日期:2011-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03039-13
更新日期:2014-04-01 00:00:00
abstract::Envelope glycoprotein E(rns) of classical swine fever virus (CSFV) has been shown to contain RNase activity and is involved in virus infection. Two short regions of amino acids in the sequence of E(rns) are responsible for RNase activity. In both regions, histidine residues appear to be essential for catalysis. They w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.151-157.1998
更新日期:1998-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3664-3674.2005
更新日期:2005-03-01 00:00:00
abstract::The polyomavirus proteins VP1, VP2, and VP3 move from their cytoplasmic site of synthesis into the nucleus, where virus assembly occurs. To identify cellular or viral components which might control this process, we determined the distribution of VP1, VP2, and VP3 in a soluble fraction, a cytoplasmic cytoskeleton fract...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.516-525.1987
更新日期:1987-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.9.4498-4507.2004
更新日期:2004-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4731-4738.2003
更新日期:2003-04-01 00:00:00
abstract::In order to study retroviral variation, selection, and viral correlates of in vivo pathogenicity, we documented the evolution of feline leukemia virus (FeLV) variants in cats that died with thymic lymphoma after infection with molecularly cloned subgroup A FeLV. Using genomic DNA from cat necropsy samples, we employed...
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pub_type: 杂志文章
doi:10.1128/JVI.68.4.2458-2467.1994
更新日期:1994-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-06-13 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.673-684.2003
更新日期:2003-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12140-12146.2004
更新日期:2004-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.1983-1991.2005
更新日期:2005-02-01 00:00:00
abstract::The alphaherpesvirus proteins UL31 and UL34 and their homologues in other herpesvirus subfamilies cooperate at the nuclear membrane in the export of nascent herpesvirus capsids. We studied the respective betaherpesvirus proteins M53 and M50 in mouse cytomegalovirus (MCMV). Recently, we established a random approach to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00681-10
更新日期:2010-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.6.1164-1167.1967
更新日期:1967-12-01 00:00:00
abstract:UNLABELLED:Previously, we reported that a mutant of Tat referred to as Nullbasic inhibits HIV-1 reverse transcription although the mechanism of action is unknown. Here we show that Nullbasic is a reverse transcriptase (RT) binding protein that targets the reverse transcription complex rather than directly inhibiting RT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03440-14
更新日期:2015-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00110-10
更新日期:2010-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.02104-06
更新日期:2007-05-01 00:00:00