Abstract:
:The UL16 protein of herpes simplex virus is capsid associated and was previously identified as a binding partner of the membrane-associated UL11 tegument protein (J. S. Loomis, R. J. Courtney, and J. W. Wills, J. Virol. 77:11417-11424, 2003). In those studies, a less-prominent, approximately 65-kDa binding partner of unknown identity was also observed. Mass spectrometry studies have now revealed this species to be UL21, a tegument protein that has been implicated in the transport of capsids in the cytoplasm. The validity of the mass spectrometry results was tested in a variety of coimmunoprecipitation and glutathione S-transferase pull-down experiments. The data revealed that UL21 and UL16 can form a complex in the absence of other viral proteins, even when the assays used proteins purified from Escherichia coli. Moreover, UL11 was able to pull down UL21 only when UL16 was present, suggesting that all three proteins can form a complex. Deletion analyses revealed that the second half of UL21 (residues 268 to 535) is sufficient for the UL16 interaction and packaging into virions; however, attempts to map a subdomain of UL16 were largely unsuccessful, with only the first 40 (of 373) residues being found to be dispensable. Nevertheless, it is clear that UL16 must have two distinct binding sites, because covalent modification of its free cysteines with N-ethylmaleimide blocked binding to UL11 but not UL21. These findings should prove useful for elucidating the molecular machinery used to transmit a signal into a virion when it attaches to cells, a recently discovered mechanism in which UL16 is a central player.
journal_name
J Viroljournal_title
Journal of virologyauthors
Harper AL,Meckes DG Jr,Marsh JA,Ward MD,Yeh PC,Baird NL,Wilson CB,Semmes OJ,Wills JWdoi
10.1128/JVI.02015-09subject
Has Abstractpub_date
2010-03-01 00:00:00pages
2963-71issue
6eissn
0022-538Xissn
1098-5514pii
JVI.02015-09journal_volume
84pub_type
杂志文章abstract::Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
abstract::We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05809-11
更新日期:2012-04-01 00:00:00
abstract::The 5'-terminal nucleotide sequence from positions 50 to 130 of vesicular stomatitis virus RNA was determined indirectly by using a defective interfering particle RNA which contains covalently linked genomic minus and antigenomic plus sense RNAs. The last 18 nucleotides of the L gene coding for in the viral polymerase...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.2.550-559.1980
更新日期:1980-05-01 00:00:00
abstract::The human papillomavirus 1 (HPV-1) virion is composed of two virally encoded proteins: a 57,000-molecular-weight polypeptide (57K polypeptide), which is the product of the L1 open reading frame (ORF), and a 78K polypeptide, which is derived from the L2 ORF. The 57K (L1) product, which represents the major structural c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2793-2799.1987
更新日期:1987-09-01 00:00:00
abstract:UNLABELLED:Broadly neutralizing antibodies (bNAbs) specific for conserved epitopes on the HIV-1 envelope (Env) are believed to be essential for protection against multiple HIV-1 clades. However, vaccines capable of stimulating the production of bNAbs remain a major challenge. Given that polyreactivity and autoreactivit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02904-14
更新日期:2015-04-01 00:00:00
abstract::Viral recombination has been postulated to play two roles in the development of human immunodeficiency virus (HIV) resistance to antiretroviral drugs. First, recombination has the capacity to associate resistance mutations expressed by distinct viruses, thereby contributing to the development of viruses with improved ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00083-07
更新日期:2007-07-01 00:00:00
abstract::We have investigated the effect of Xenopus laevis p53 (Xp53) on transcription from a variety of promoters which are regulated by mouse p53 using a chloramphenicol acetyltransferase reporter system. Although Xp53 transactivated promoters that are up-regulated by mouse p53, it was unable to cause repression. This abilit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7178-7187.1994
更新日期:1994-11-01 00:00:00
abstract:UNLABELLED:In vitro, infection of polarized human intestinal epithelial cells by coxsackievirus B3 (CVB3) depends on virus interaction with decay-accelerating factor (DAF), a receptor expressed on the apical cell surface. Although mice are highly susceptible to CVB3 infection when virus is delivered by intraperitoneal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03468-14
更新日期:2015-04-01 00:00:00
abstract::Epstein-Barr virus (EBV), a member of human gammaherpesvirus, infects mainly B cells. EBV has two alternative life cycles, latent and lytic, and is reactivated occasionally from the latent stage to the lytic cycle. To combat EBV-associated disorders, understanding the molecular mechanisms of the EBV lytic replication ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00975-17
更新日期:2017-11-14 00:00:00
abstract::Passive administration of porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing antibodies (NAbs) can effectively protect pigs against PRRSV infection. However, after PRRSV infection, pigs typically develop a weak and deferred NAb response. One major reason for such a meager NAb response is the phen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00189-11
更新日期:2011-06-01 00:00:00
abstract::Retinoid (vitamin A) is an essential diet constituent that governs a broad range of biological processes. Its biologically active metabolite, all-trans retinoic acid (ATRA), exhibits a potent antiviral property by enhancing both innate and adaptive antiviral immunity against a variety of viral pathogens, such as, but ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02302-18
更新日期:2019-04-03 00:00:00
abstract::The experimental infection of newborn calves with bovine norovirus was used as a homologous large animal model to study the pathogenesis of norovirus infection and to determine target cells for viral replication. Six newborn calves were inoculated orally with Jena virus (JV), a bovine norovirus GIII.1 strain, and six ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05342-11
更新日期:2011-11-01 00:00:00
abstract::Long interspersed nuclear element 1 (LINE-1; L1) retrotransposons are the most common retroelements in mammalian genomes. Unlike individual families of endogenous retroviruses (ERVs), they have remained active throughout the mammalian radiation and are responsible for most of the retroelement movement and much genome ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05180-11
更新日期:2011-12-01 00:00:00
abstract::In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.596-603.1976
更新日期:1976-05-01 00:00:00
abstract::We have devised an enrichment scheme for the isolation of export-competent derivatives of pseudorabies virus glycoprotein gIII signal peptide mutants. Enrichment is based upon a growth advantage imparted upon gIII-containing virions compared with virions lacking the glycoprotein. Each of identified derivatives suppres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.2787-2794.1994
更新日期:1994-05-01 00:00:00
abstract:UNLABELLED:Ebola virus (EBOV) belongs to the group of nonsegmented negative-sense RNA viruses. The seven EBOV genes are separated by variable gene borders, including short (4- or 5-nucleotide) intergenic regions (IRs), a single long (144-nucleotide) IR, and gene overlaps, where the neighboring gene end and start signal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01863-14
更新日期:2014-11-01 00:00:00
abstract::Regulatory T (T(R)) cells maintain tolerance to self-antigens and control immune responses to alloantigens after organ transplantation. Here, we show that CD4(+) CD25(+) human T(R) cells suppress virus-specific T-cell responses. Depletion of T(R) cells from peripheral blood mononuclear cells enhances T-cell responses ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2454-2459.2004
更新日期:2004-03-01 00:00:00
abstract::Mutations at amino acids 143, 148, and 155 in HIV-1 integrase (IN) define primary resistance pathways in subjects failing raltegravir (RAL)-containing treatments. Although each pathway appears to be genetically distinct, shifts in the predominant resistant virus population have been reported under continued drug press...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06618-11
更新日期:2012-07-01 00:00:00
abstract:UNLABELLED:Intron-containing mRNAs are subject to restricted nuclear export in higher eukaryotes. Retroviral replication requires the nucleocytoplasmic transport of both spliced and unspliced RNA transcripts, and RNA export mechanisms of gammaretroviruses are poorly characterized. Here, we report the involvement of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03584-13
更新日期:2014-04-01 00:00:00
abstract::We have examined the attachment and penetration phenotypes of several glycoprotein gIII mutants of pseudorabies virus (PRV) and have identified the first one-third of gIII as a region that mediates efficient virus attachment to PK15 and Vero cells. This portion of gIII, amino acids 25 through 157 of the wild-type sequ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2646-2654.1993
更新日期:1993-05-01 00:00:00
abstract::The human herpes simplex virus (HSV) protein VP16 induces formation of a transcriptional regulatory complex with two cellular factors-the POU homeodomain transcription factor Oct-1 and the cell proliferation factor HCF-1-to activate viral immediate-early-gene transcription. Although the cellular role of Oct-1 in trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.24.12402-12411.2001
更新日期:2001-12-01 00:00:00
abstract::The major envelope glycoproteins gp120 and gp41 of human immunodeficiency virus type 1, the causative agent for human AIDS, contain numerous N-linked oligosaccharides. We report here our discovery that N-acetylglucosamine residues within the complex-type N-linked oligosaccharides of both gp120 and its precursor, gp160...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.943-952.1993
更新日期:1993-02-01 00:00:00
abstract::Rotavirus plus-strand RNAs not only direct protein synthesis but also serve as templates for the synthesis of the segmented double-stranded RNA (dsRNA) genome. In this study, we identified short-interfering RNAs (siRNAs) for viral genes 5, 8, and 9 that suppressed the expression of NSP1, a nonessential protein; NSP2, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7763-7774.2004
更新日期:2004-07-01 00:00:00
abstract:UNLABELLED:Foreskin is the principal site of heterosexual HIV-1 infection in men. However, little is known about HIV-1-specific immune responses or inflammation in foreskin. To the best of our knowledge, no previous studies have assessed immune responses to candidate HIV-1 vaccines in foreskin. Using the rhesus monkey ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03771-13
更新日期:2014-04-01 00:00:00
abstract::Autophagy is an intracellular degradation pathway that provides a host defense mechanism against intracellular pathogens. However, many viruses exploit this mechanism to promote their replication. This study shows that lytic induction of Epstein-Barr virus (EBV) increases the membrane-bound form of LC3 (LC3-II) and LC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02033-14
更新日期:2014-10-01 00:00:00
abstract::We describe here the neurovirulence properties of a herpes simplex virus type 1 gamma34.5 second-site suppressor mutant. gamma34.5 mutants are nonneurovirulent in animals and fail to grow in a variety of cultured cells due to a block at the level of protein synthesis. Extragenic suppressors with restored capacity to r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5189-5196.2001
更新日期:2001-06-01 00:00:00
abstract:UNLABELLED:Uracil DNA glycosylases (UNG) are highly conserved proteins that preserve DNA fidelity by catalyzing the removal of mutagenic uracils. All herpesviruses encode a viral UNG (vUNG), and yet the role of the vUNG in a pathogenic course of gammaherpesvirus infection is not known. First, we demonstrated that the v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03111-14
更新日期:2015-03-01 00:00:00
abstract::In most prion diseases, infectivity accumulates in lymphoreticular organs early after infection. Defects in hematopoietic compartments, such as impaired B-cell maturation, or in stromal compartments, such as abrogation of follicular dendritic cells, can delay or prevent lymphoreticular prion colonization. However, the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.7097-7106.2001
更新日期:2001-08-01 00:00:00
abstract::The mutational alterations in polyoma virus mutants ts-a and ts-25E which cause their large T-antigens to be thermolabile have been identified. In ts-a, a G leads to A transition at nucleotide 2193 causes the replacement of Ala (GCT) by Thr (ACT). In ts-25E, a G leads to T transversion at nucleotide 2883 causes the re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.1094-1098.1981
更新日期:1981-03-01 00:00:00
abstract::We have developed a means to identify genes associated with particular aspects of virulence. By beginning with an avirulent deletion mutant of rabbitpox virus and systematically reintroducing overlapping segments of the deleted region, we have identified two regions of the viral genome associated with increased virule...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1530-1542.1991
更新日期:1991-03-01 00:00:00