Abstract:
:In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted transcriptions of the group I RNA-ts mutants. NS protein activity, but not L protein activity, was purified from the group I ts mutants; this NS fraction always displayed the wt phenotype in reconstitution assays. Neither the L nor the NS protein was capable of restoring the defective transcriptive activity of the group IV vesicular stomatitis virus mutant ts W16B.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hunt DM,Emerson SU,Wagner RRdoi
10.1128/JVI.18.2.596-603.1976subject
Has Abstractpub_date
1976-05-01 00:00:00pages
596-603issue
2eissn
0022-538Xissn
1098-5514journal_volume
18pub_type
杂志文章abstract::The binding of tail parts to the head of phage P22 is normally irreversible, but after adsorption to host cells sonication releases many of the tail parts intact. This release is dependent on the power of sonication, but is independent of the length of sonication from 2 to 32 s. This phenomenon has been used to show t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.2.675-677.1976
更新日期:1976-02-01 00:00:00
abstract::The human genome encodes over 500 microRNAs (miRNAs), small RNAs (19 to 26 nucleotides [nt]) that regulate the expressions of diverse cellular genes. Many cellular processes are altered through a variety of mechanisms by human cytomegalovirus (HCMV) infection. We asked whether HCMV infection leads to changes in the ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00961-08
更新日期:2008-09-01 00:00:00
abstract::RNA structures play key roles in the replication of RNA viruses. Sequence alignment software, thermodynamic RNA folding programs, and classical comparative phylogenetic analysis were used to build models of six RNA elements in the coding region of the hepatitis C virus (HCV) RNA-dependent RNA polymerase, NS5B. The imp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1352-1366.2004
更新日期:2004-02-01 00:00:00
abstract::We identified the transcriptional coactivator FHL2 as a novel target of the human papillomavirus type 16 (HPV-16) E7 oncoprotein, which plays a major role in cell transformation. The interaction with FHL2 is abolished by mutations in conserved regions 1 and 2 and in the C-terminal zinc finger domain of E7, all require...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01699-06
更新日期:2007-01-01 00:00:00
abstract::The gene encoding glycoprotein B (gB) of murine cytomegalovirus (MCMV) strain Smith was identified, sequenced, and expressed by recombinant vaccinia virus. The gB gene was found adjacent to the polymerase gene, as it is in the genome of human cytomegalovirus (HCMV). The open reading frame consists of 2,784 nucleotides...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4399-4406.1992
更新日期:1992-07-01 00:00:00
abstract::We describe the use of herpesvirus promoters to regulate the expression of a Sindbis virus replicon (SINrep/LacZ). We isolated cell lines that contain the cDNA of SINrep/LacZ under the control of a promoter from a herpesvirus early gene which requires regulatory proteins encoded by immediate-early genes for expression...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1998-2005.1999
更新日期:1999-03-01 00:00:00
abstract::Mosquito-borne alphaviruses, which replicate alternately and obligately in mosquitoes and vertebrates, appear to experience lower rates of evolution than do many RNA viruses that replicate solely in vertebrates. This genetic stability is hypothesized to result from the alternating host cycle, which constrains evolutio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14253-14260.2005
更新日期:2005-11-01 00:00:00
abstract::We previously reported that human cytomegalovirus (HCMV) infection induces endoplasmic reticulum (ER) stress, resulting in activation of the unfolded protein response (UPR). Although some normal consequences of UPR activation (e.g., translation attenuation) are detrimental to viral infection, we have previously shown ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15388-15397.2005
更新日期:2005-12-01 00:00:00
abstract:UNLABELLED:Hepatitis C virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma in humans. We showed previously that HCV induces autophagy for viral persistence by preventing the innate immune response. Knockdown of autophagy reduces extracellular HCV release, although the precis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02383-15
更新日期:2015-11-18 00:00:00
abstract::The proline-rich tandem repeat domain of human mucin MUC1 forms an extended structure containing large repeating loops that are crested by a turn. We show that the repeating-loop structure of MUC1 can be replaced by an antibody complementarity-determining region loop of a human immunodeficiency virus type 1 (HIV-1)-sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6557-6562.1996
更新日期:1996-10-01 00:00:00
abstract::We tested the ability of human monoclonal antibodies (immunoglobulin G1b12 [IgG1b12] and 19b) and CD4-based molecules (CD4-IgG2 and soluble CD4 [sCD4]) to neutralize human immunodeficiency virus type 1 directly from the plasma of seropositive donors in an ex vivo neutralization assay. IgG1b12 and CD4-IgG2, at concentr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2586-2592.1996
更新日期:1996-04-01 00:00:00
abstract::Viruses are considered to use vesicular trafficking in infected cells, but the details of assembly/release pathways of hepatitis B virus (HBV) are still unknown. To identify key regulators of HBV production, we performed short interfering RNA (siRNA) screening for Rab proteins, which are considered to act as molecular...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00621-19
更新日期:2019-07-17 00:00:00
abstract::The Kaposi's sarcoma-associated herpesvirus nuclear egress complex is composed of two proteins, ORF67 and ORF69. In this study, we have recapitulated the KSHV complex by coexpression of these two proteins in insect cells using expression from recombinant baculoviruses. The proteins form a complex at the nuclear membra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05988-11
更新日期:2012-01-01 00:00:00
abstract::The association between the level of class I major histocompatibility (MHC) antigen expression and the tumorigenic phenotype was determined for cells from a series of 15 lines of adenovirus type 2 (Ad2)-, Ad12-, and simian virus 40 (SV40)-transformed hamster cells and 16 lines of cells established from hamster tumors ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2755-2761.1988
更新日期:1988-08-01 00:00:00
abstract::We determined the sequence of the gene for the virion transactivator protein Vmw65 of herpes simplex virus type 2 (HSV-2), strain 333. An analysis of the coding sequence revealed an overall high degree of primary sequence conservation (86%) relative to the HSV-1 protein, although the carboxy-terminal region which enco...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6705-6713.1991
更新日期:1991-12-01 00:00:00
abstract::The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.995-998.2004
更新日期:2004-01-01 00:00:00
abstract::Treatment of patients with lamivudine (3TC) results in loss of detectable levels of hepatitis B virus (HBV) DNA from serum; however, the relapse rate, with regard to both reappearance of virus in the bloodstream and hepatic inflammation, is high when therapy is terminated. Although the rebound observed in patients has...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8148-8160.2002
更新日期:2002-08-01 00:00:00
abstract::The varicella-zoster virus (VZV) open reading frame 54 (ORF54) gene encodes an 87-kDa monomer that oligomerizes to form the VZV portal protein, pORF54. pORF54 was hypothesized to perform a function similar to that of a previously described herpes simplex virus 1 (HSV-1) homolog, pUL6. pUL6 and the associated viral ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00376-14
更新日期:2014-07-01 00:00:00
abstract::Treatment of human immunodeficiency virus type 1 (HIV-1)-infected CEM cell cultures with escalating concentrations of the quinoxaline S-2720 resulted in an ordered appearance of single and multiple mutant virus strains that gradually became resistant to the quinoxaline and other nonnucleoside reverse transcriptase (RT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8195-8203.1997
更新日期:1997-11-01 00:00:00
abstract::The Epstein-Barr virus (EBV) is an oncogenic human herpesvirus. EBV latent membrane protein 1 (LMP-1) is a viral oncogene that manifests its oncogenic phenotype through activation of cellular signaling pathways involved in cell growth, survival, differentiation, and transformation. Lytic LMP-1 (lyLMP-1) is a related E...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00180-06
更新日期:2006-08-01 00:00:00
abstract::MVL2, an enveloped double-stranded DNA mycoplasmavirus, causes a nonlytic infection of Acholeplasma laidlawii leading to the establishment of a persistent infection. Persistently infected clones were found to be resistant to superinfection by homologous virus, but could be infected by heterologous virus. Cells in a pe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.254-261.1978
更新日期:1978-10-01 00:00:00
abstract::The affinity of human immunodeficiency virus (HIV) envelope for CD4 and CCR5 appears to be associated with aspects of R5 virus (virus using the CCR5 coreceptor) pathogenicity. However, entry efficiency results from complex interactions between the viral envelope glycoprotein and both CD4 and CCR5, which limits attempt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01242-09
更新日期:2009-11-01 00:00:00
abstract::Initiation of herpesvirus infection requires attachment of virions to the host cell followed by fusion of virion envelope and cellular cytoplasmic membrane during penetration. In several alphaherpesviruses, glycoprotein C (gC) is the primary attachment protein, interacting with cell-surface heparan sulfate proteoglyca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7341-7348.1998
更新日期:1998-09-01 00:00:00
abstract::Human papillomavirus type 1 (HPV1) E4 protein is associated with cytoplasmic and nuclear inclusions in productively infected keratinocytes. Here we have used transient expression of HPV1 E4 (also known as E1E4) protein in keratinocytes to reproduce formation of E4 inclusions. Immunofluorescence analysis showed that pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.673-684.2003
更新日期:2003-01-01 00:00:00
abstract::The herpes simplex virus type 1 genome contains three origins of DNA replication: two copies of oriS and one copy of oriL. Although oriS has been characterized extensively, characterization of oriL has been severely limited by the inability to amplify oriL sequences in an undeleted form in Escherichia coli. We report ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1377-1388.1995
更新日期:1995-03-01 00:00:00
abstract::Some picornaviruses, for example, poliovirus, increase bidirectional permeability of the nuclear envelope and suppress active nucleocytoplasmic transport. These activities require the viral protease 2A(pro). Here, we studied nucleocytoplasmic traffic in cells infected with encephalomyocarditis virus (EMCV; a cardiovir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.6.2705-2717.2006
更新日期:2006-03-01 00:00:00
abstract::Infection of HeLa cells with adenovirus serotype 2 causes rearrangements in nuclear matrix morphology which can best be seen by gentle cell extraction and embedment-free section electron microscopy. We used these techniques to examine the nuclear matrices and cytoskeletons of cells at 6, 13, 28, and 44 h after infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.1007-1018.1987
更新日期:1987-04-01 00:00:00
abstract::A substantial number of viruses have been demonstrated to subvert autophagy to promote their own replication. Recent publications have reported the proviral effect of autophagy induction on hepatitis B virus (HBV) replication. Hepatitis delta virus (HDV) is a defective virus and an occasional obligate satellite of HBV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01936-19
更新日期:2020-01-31 00:00:00
abstract::Viruses encode proteins that disrupt chemokine responses. The murine gammaherpesvirus 68 gene M3 encodes a chemokine binding protein (vCKBP-3) which has no sequence similarity to chemokine receptors but inhibits chemokine receptor binding and activity. We have used a panel of CXCL8 analogs to identify the structural r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8588-8592.2003
更新日期:2003-08-01 00:00:00
abstract:UNLABELLED:Herpes simplex virus 1 (HSV-1) most commonly causes recrudescent labial ulcers; however, it is also the leading cause of infectious blindness in developed countries. Previous research in animal models has demonstrated that the severity of HSV-1 ocular disease is influenced by three main factors: host innate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00821-16
更新日期:2016-08-26 00:00:00