Abstract:
:We tested the ability of human monoclonal antibodies (immunoglobulin G1b12 [IgG1b12] and 19b) and CD4-based molecules (CD4-IgG2 and soluble CD4 [sCD4]) to neutralize human immunodeficiency virus type 1 directly from the plasma of seropositive donors in an ex vivo neutralization assay. IgG1b12 and CD4-IgG2, at concentrations from 1 to 25 micrograms/ml, were found to be effective at reducing the HIV-1 titer in most plasma samples. When viruses recovered from plasma samples were expanded to produce virus stocks, no correlation between the neutralization sensitivities to IgG1b12 and CD4-IgG2 of the in vitro passaged stocks and those of the ex vivo neutralizations performed directly on the plasma was observed. These differences could be due to changes in neutralization sensitivity that occur after one passage of the virus in vitro, or they could be related to the presence of complement or antibodies in the plasma. Furthermore, differences in expression of adhesion molecules on plasma-derived and phytohemagglutinin-activated peripheral blood mononuclear cell-derived viruses could be involved. These studies suggest that IgG1b12 and CD4-IgG2 have broad and potent neutralizing activity in both in vitro and ex vivo neutralization assays and should be considered for use as potential immunoprophylactic or therapeutic agents.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gauduin MC,Allaway GP,Maddon PJ,Barbas CF 3rd,Burton DR,Koup RAdoi
10.1128/JVI.70.4.2586-2592.1996subject
Has Abstractpub_date
1996-04-01 00:00:00pages
2586-92issue
4eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::The host cell regulators and substrates of the Rous sarcoma virus transforming protein pp60v-src remain largely unknown. Viral mutants which induce a host-dependent phenotype may result from mutations which affect the interaction of pp60v-src with host cell components. To isolate such mutants and to examine the role o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.542-554.1989
更新日期:1989-02-01 00:00:00
abstract::T cells are central players in the immune response to infectious disease, with the specificity of their responses controlled by the T-cell receptor (TCR)/CD3 complex on the cell surface. Impairment of TCR/CD3-directed CD4(+) T-cell immune responses is frequently observed in individuals infected with human immunodefici...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.5207-5213.1999
更新日期:1999-06-01 00:00:00
abstract::A mouse model of high-risk human papillomavirus infection was developed in which human papillomavirus (HPV) type 16 DNA was inoculated into human foreskin grafted to the skin of severe combined immunodeficient (scid) mice. Grafted skin contained human epidermis and dermis and, like normal human skin, expressed involuc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2716-2721.1995
更新日期:1995-04-01 00:00:00
abstract::The genomes of influenza A viruses consist of eight negative-strand RNA segments. Recent studies suggest that influenza viruses are able to specifically package their segmented genomes into the progeny virions. Segment-specific packaging signals of influenza virus RNAs (vRNAs) are located in the 5' and 3' noncoding re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00662-12
更新日期:2012-07-01 00:00:00
abstract::Borna disease virus infection of neonatal rats results in a characteristic behavioral syndrome and apoptosis of subsets of neurons in the hippocampus and cerebellum (neonatal Borna disease [NBD]). The cellular mechanisms leading to neurodevelopmental damage in NBD have not been fully elucidated. Insights into this mod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00836-06
更新日期:2006-09-01 00:00:00
abstract::The hepadnavirus reverse transcriptase is a multifunction enzyme. In addition to its role in DNA synthesis, the polymerase is required for RNA packaging and also functions as the primer for minus-strand DNA synthesis. Previously, we demonstrated that the protein-priming activity of the polymerase requires a viral RNA ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.8437-8442.1994
更新日期:1994-12-01 00:00:00
abstract::Nonhuman primates are widely used to study correlates of protective immunity in AIDS research. Successful cellular immune responses have been difficult to identify because heterogeneity within macaque major histocompatibility complex (MHC) genes results in quantitative and qualitative differences in immune responses. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01841-06
更新日期:2007-01-01 00:00:00
abstract::A nucleotide phosphohydrolase is firmly associated with a purified polyhedral cytoplasmic deoxyvirus, frog virus 3. This adenosine triphosphatase is distinguishable from known mammalian cell adenosine triphosphatases and from adenosine triphosphatase of an unrelated cytoplasmic replicating virus grown in the same host...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.5.619-624.1971
更新日期:1971-05-01 00:00:00
abstract::RNA viruses within a host exist as dynamic distributions of closely related mutants and recombinant genomes. These closely related mutants and recombinant genomes, which are subjected to a continuous process of genetic variation, competition, and selection, act as a unit of selection, termed viral quasispecies. Charac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00950-09
更新日期:2009-12-01 00:00:00
abstract::Many viral fusion proteins are primed by proteolytic cleavage near their fusion peptides. While the coronavirus (CoV) spike (S) protein is known to be cleaved at the S1/S2 boundary, this cleavage site is not closely linked to a fusion peptide. However, a second cleavage site has been identified in the severe acute res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00079-09
更新日期:2009-08-01 00:00:00
abstract::There is an incomplete understanding of the differences between neonatal immune responses that contribute to the increased susceptibility of neonates to some viral infections. We tested the hypothesis that neonates are more susceptible than adults to mouse adenovirus type 1 (MAV-1) respiratory infection and are impair...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06967-11
更新日期:2012-04-01 00:00:00
abstract:UNLABELLED:SSV-type integrases, encoded by fuselloviruses which infect the hyperthermophilic archaea of the Sulfolobales, are archaeal members of the tyrosine recombinase family. These integrases catalyze viral integration into and excision from a specific site on the host genome. In the present study, we have establis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01637-15
更新日期:2015-11-01 00:00:00
abstract::Adeno-associated virus (AAV) is a single-stranded DNA virus with a unique biphasic lifestyle consisting of both a productive and a latent phase. Typically, the productive phase requires coinfection with a helper virus, for instance adenovirus, while the latent phase dominates in healthy cells. In the latent state, AAV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.7874-7882.2004
更新日期:2004-08-01 00:00:00
abstract::The tegument of herpesviruses is a highly complex structural layer between the nucleocapsid and the envelope of virions. Tegument proteins play both structural and regulatory functions during replication and spread, but the interactions and functions of many of these proteins are poorly understood. Here we focus on tw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02196-16
更新日期:2017-01-03 00:00:00
abstract::Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. In order to systematically investigate the role of the membrane-proximal residues in the M2 cytoplasmic tail in virus replication, we utilized scanning and directed alanine mutagenesis in combination wit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05970-11
更新日期:2011-12-01 00:00:00
abstract::The core gene of hepatitis B virus contains two in-phase AUG codons which may both be used in the viral life cycle. By in vitro translation of transcripts produced in vitro, we investigated the corresponding core gene products and their counterparts in vivo. Depending on the location of the 5' end of the transcripts, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3109-3113.1987
更新日期:1987-10-01 00:00:00
abstract::We have cloned several prototypic members of the family of human endogenous retroviruslike elements having a histidine tRNA primer-binding site (RTVL-H) and have determined the nucleotide sequence of one of these clones (RTVL-H2). The RTVL-H2 sequence is 5,813 nucleotides long, with long terminal repeats of 450 nucleo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4060-4066.1987
更新日期:1987-12-01 00:00:00
abstract::Human erythroid progenitor cells are the main target cells of the human parvovirus B19 (B19), and B19 infection induces a transient erythroid aplastic crisis. Several authors have reported that the nonstructural protein 1 (NS-1) encoded by this virus has a cytotoxic effect, but the underlying mechanism of NS-1-induced...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8762-8770.1999
更新日期:1999-10-01 00:00:00
abstract::SARS coronavirus (SARS-CoV) encodes several unique group-specific open reading frames (ORFs) relative to other known coronaviruses. To determine the significance of the SARS-CoV group-specific ORFs in virus replication in vitro and in mice, we systematically deleted five of the eight group-specific ORFs, ORF3a, OF3b, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14909-14922.2005
更新日期:2005-12-01 00:00:00
abstract:UNLABELLED:Foreskin is the principal site of heterosexual HIV-1 infection in men. However, little is known about HIV-1-specific immune responses or inflammation in foreskin. To the best of our knowledge, no previous studies have assessed immune responses to candidate HIV-1 vaccines in foreskin. Using the rhesus monkey ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03771-13
更新日期:2014-04-01 00:00:00
abstract::A uniform nomenclature has been agreed upon for monoclonal antibodies directed against virus-coded proteins of simian virus 40 and polyoma virus. Blocks of numbers from PAb1 to PAb999 have been allocated to workers involved in the isolation of monoclonal antibodies of this type. The correspondence between PAb numbers ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.709-709.1982
更新日期:1982-02-01 00:00:00
abstract::A series of contiguous 30-bp deletions were introduced into the regions upstream of the p19 and p40 promoters of adeno-associated virus (AAV), and the effects of these deletions on induction of AAV transcription by the rep gene products was evaluated. A novel complementation system was devised for supplying wild-type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.2936-2945.1991
更新日期:1991-06-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a cluster of 12 microRNAs (miRNAs) that are processed from a transcript that is embedded within the major latency control region. We have generated a deletion mutation that eliminates 10 of the 12 viral miRNAs from the KSHV bacmid by using recombineering methods. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-09
更新日期:2010-03-01 00:00:00
abstract::Temperature-sensitive (ts) mutants of vesicular stomatitis virus belonging to complementation groups I, II and IV inhibited the replication of wild-type vesicular stomatitis virus when mixed infections were carried out in BHK21 cells at 32, 37, and 39.5 C. The group IV mutant (ts G 41) was most effective in this regar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.1.102-107.1976
更新日期:1976-07-01 00:00:00
abstract::V3-glycan-targeting broadly neutralizing antibodies (bNAbs) are a focus of HIV-1 vaccine development. Understanding the viral dynamics that stimulate the development of these antibodies can provide insights for immunogen design. We used a deep-sequencing approach, together with neutralization phenotyping, to investiga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00828-17
更新日期:2017-08-24 00:00:00
abstract:UNLABELLED:In vitro, infection of polarized human intestinal epithelial cells by coxsackievirus B3 (CVB3) depends on virus interaction with decay-accelerating factor (DAF), a receptor expressed on the apical cell surface. Although mice are highly susceptible to CVB3 infection when virus is delivered by intraperitoneal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03468-14
更新日期:2015-04-01 00:00:00
abstract::The complete nucleotide sequences of the vesicular stomatitis virus mRNA's encoding the glycoprotein (G) and the matrix protein (M) have been determined from cDNA clones that contain the complete coding sequences from each mRNA. The G protein mRNA is 1,665 nucleotides long, excluding polyadenylic acid, and encodes a p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.519-528.1981
更新日期:1981-08-01 00:00:00
abstract::Adeno-associated virus (AAV) is a member of the Parvoviridae, belonging to the Dependovirus genus. Currently, several distinct isolates of AAV are in development for use in human gene therapy applications due to their ability to transduce different target cells. The need to manipulate AAV capsids for specific tissue d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.5047-5058.2005
更新日期:2005-04-01 00:00:00
abstract::We report the identification of a functional nuclear localization signal (NLS) in the human cytomegalovirus (HCMV) large tegument protein pUL48 that is required for nuclear localization in transfected cells and is essential for viral growth. The NLS was mapped to pUL48 amino acid residues 284 to 302. This sequence con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03558-12
更新日期:2013-05-01 00:00:00
abstract::Poliovirus inactivation by ammonia causes a slight reduction in the sedimentation coefficients of viral particles, but has no detectable effect on either the electrophoretic pattern of viral capsid proteins or the isoelectric points of inactivated particles. These virions still attach to cells, but are unable to repre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.299-305.1978
更新日期:1978-05-01 00:00:00