Abstract:
:A series of contiguous 30-bp deletions were introduced into the regions upstream of the p19 and p40 promoters of adeno-associated virus (AAV), and the effects of these deletions on induction of AAV transcription by the rep gene products was evaluated. A novel complementation system was devised for supplying wild-type Rep protein when mutations disrupted the trans activation activity of the Rep protein. Transcription from the p40 promoter was eliminated upon deletion of the TATA sequence located between -4 and -33 from the cap site. Deletions which removed sequences from -34 to -123 bp from the p40 mRNA start site substantially reduced Rep induction of p40 transcription. p19 transcription was also undetectable when the p19 TATA sequence between -4 and -33 was deleted. In contrast to the p40 region, two types of cis-active sequences were found associated with the p19 promoter. Sequences between -4 and -63 bp relative to the p19 cap site were essential for Rep induction only from the p19 promoter. Deletions between -94 and -153 bp relative to the p19 cap site reduced Rep induction of both the p19 and p40 promoters coordinately. These two noncontiguous regions were separated by a 30-bp sequence that was not essential for transcription control. Further deletion analysis delineated a second cis-active element, associated with the p5 promoter (AAV nucleotides 191 to 320), which was also necessary for coordinate Rep activation of both the p19 and p40 promoters. Finally, the dependence of p40 transcription on the Rep-responsive elements within the p5 and p19 regions could be overcome by the presence of the AAV terminal repeats, suggesting that the terminal repeats contained redundant Rep-responsive elements. These results implied an interdependence in cis between the three AAV promoters and suggested a novel mechanism for coordinate regulation of gene expression in response to the trans-activating Rep protein. Coordinate induction appeared to be the result of a simultaneous interaction between the Rep protein and sequence elements associated with two or all three of the AAV promoters.
journal_name
J Viroljournal_title
Journal of virologyauthors
McCarty DM,Christensen M,Muzyczka Ndoi
10.1128/JVI.65.6.2936-2945.1991subject
Has Abstractpub_date
1991-06-01 00:00:00pages
2936-45issue
6eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
杂志文章abstract::Particular interest in human T lymphocyte lymphoma/leukemia virus (HTLV) derives from the close association of HTLV with several types of human mature T lymphocyte malignancies and the strong possibility that HTLV is the causative agent of this group of leukemias and lymphomas. This is the first report to show that HT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.2.440-446.1985
更新日期:1985-02-01 00:00:00
abstract::Alpha/beta interferon (IFN-alpha/beta) protects the host from virus infection by inhibition of lytic virus replication in infected cells and modulation of the antiviral cell-mediated immune response. To determine whether IFN-alpha/beta also modulates the virus-host interaction during latent virus infection, we infecte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14149-14160.2005
更新日期:2005-11-01 00:00:00
abstract::We investigated the protective role of antibodies in vaginal secretions of mice that were immune to vaginal challenge with herpes simplex virus type 2 (HSV-2). Unfractionated vaginal immunoglobulins from immune and nonimmune mice and affinity-purified immunoglobulin G (IgG) and secretory IgA (S-IgA) from immune secret...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8109-8115.1997
更新日期:1997-11-01 00:00:00
abstract::It is shown that the plasmid prophage P1 can be displaced by a superinfecting P1 phage in Escherichia coli recA(P1) lysogens. Six widely separated phage markers were used to distinguish between residual recombination and total substitution. It is further shown that superinfection of recA lysogens can lead to loss of b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.2.277-280.1979
更新日期:1979-08-01 00:00:00
abstract::The mammalian tripartite motif protein, TRIM5α, recognizes retroviral capsids entering the cytoplasm and blocks virus infection. Depending on the particular TRIM5α protein and retrovirus, complete disruption of the TRIM5α RING domain decreases virus-restricting activity to various degrees. TRIM5α exhibits RING domain-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00341-11
更新日期:2011-08-01 00:00:00
abstract::Intramuscular administration of inactivated influenza virus vaccine is the main vaccine platform used for the prevention of seasonal influenza virus infection. In clinical trials, inactivated H5N1 vaccines have been shown to be safe and capable of eliciting immune correlates of protection. However, the H5N1 vaccines a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00596-10
更新日期:2010-09-01 00:00:00
abstract::Macrophage adherence, an important regulatory signal, has the potential to affect human immunodeficiency virus (HIV) production either directly or by priming monocytes to respond to other activating signals. We have investigated the role of adherence as an activator of HIV-1 transcription and release. The effects of a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3569-3575.1993
更新日期:1993-06-01 00:00:00
abstract::Primary human embryonic kidney (HEK) cells were transformed by a focus assay with BK virus (BKV) DNA molecularly cloned at its unique EcoRI site. Both viral DNA sequences and viral tumor antigens were present and expressed in all the foci that we examined. However, cells isolated from foci were incapable of growth in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.680-683.1986
更新日期:1986-05-01 00:00:00
abstract::Most patients infected with hepatitis C virus (HCV) become chronic carriers. Viruses that efficiently establish persistent infections must have effective ways of evading host defenses. In the case of HCV, little is known about how chronic infections are established or maintained. Besides hepatocytes, several reports s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.772-781.2001
更新日期:2001-01-01 00:00:00
abstract::Human immunodeficiency virus clade C (HIV-C) accounts for >56% of all HIV infections worldwide. To investigate vaccine safety and efficacy in nonhuman primates, a pathogenic, R5-tropic, neutralization-sensitive simian-human immunodeficiency virus (SHIV) carrying HIV-C env would be desirable. We have constructed SHIV-2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02066-08
更新日期:2009-02-01 00:00:00
abstract::We investigated properties of the rotavirus genome segment 11 protein. A rotavirus SA11 genome segment 11 cDNA which contains the entire coding region was sequenced and inserted into the baculovirus transfer vector pVL941. Recombinants containing gene 11 cDNA were selected, and the gene 11 product expressed in Spodopt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3974-3982.1989
更新日期:1989-09-01 00:00:00
abstract::The distribtuion of various amino acid tRNA's in the 4S RNA components of avian myeloblastosis virus (AMV) and in 4S RNA prepared from chicken cmbryo cells, chicken myeloblasts, and chicken livers was determined. This was done by aminoacylating the 4S RNA samples with a mixture of 17 radioactive amino acids and subseq...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.6.1608-1614.1975
更新日期:1975-12-01 00:00:00
abstract::Infection of cells with enveloped viruses is accomplished through membrane fusion. The binding and fusion processes are mediated by the spike proteins in the envelope of the virus particle and usually involve a series of conformational changes in these proteins. We have studied the low-pH-mediated fusion process of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7309-7318.1992
更新日期:1992-12-01 00:00:00
abstract::We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved;...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-15
更新日期:2015-07-01 00:00:00
abstract::Retinoic acid-inducible gene I (RIG-I) is a key sensor for recognizing nucleic acids derived from RNA viruses and triggers beta interferon (IFN-β) production. Because of its important role in antiviral innate immunity, the activity of RIG-I must be tightly controlled. Here, we used yeast two-hybrid screening to identi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01073-13
更新日期:2013-09-01 00:00:00
abstract::The inducible transcription factor NF-kappaB is commonly activated upon RNA virus infection and is a key player in the induction and regulation of the innate immune response. Borna disease virus (BDV) is a neurotropic negative-strand RNA virus, which replicates in the nucleus of the infected cell and causes a persiste...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6043-6051.2005
更新日期:2005-05-01 00:00:00
abstract::Picornaviruses rearrange cellular membranes to form cytosolic replication sites. In the case of poliovirus and several other picornaviruses, these membranes are derived from subversion of the cellular autophagy pathway. We also reported observation of autophagosome-like structures during infection by two human rhinovi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00316-11
更新日期:2011-09-01 00:00:00
abstract::The mammalian APOBEC3 proteins are an important component of the cellular innate immune response to retroviral infection. APOBEC3G can extinguish HIV-1 infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity that attacks the nascent viral cDNA during reverse transcription, caus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00056-10
更新日期:2010-07-01 00:00:00
abstract::The activity of human immunodeficiency virus Rev as a regulator of viral mRNA expression is tightly linked to its ability to shuttle between the nucleus and cytoplasm; these properties are conferred by a leucine-rich nuclear export signal (NES) and by an arginine-rich nuclear localization signal/RNA binding domain (NL...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11899-11910.2000
更新日期:2000-12-01 00:00:00
abstract::Treatment of purified simian virus 40 large T antigen (LT) with protein phosphatase 2A stimulates LT-dependent DNA unwinding and replication (D. M. Virshup, M. G. Kauffman, and T. J. Kelly, EMBO J. 8: 3891-3898, 1989). The specificity of the catalytic subunit of protein phosphatase 2A toward LT was investigated by two...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.2098-2101.1991
更新日期:1991-04-01 00:00:00
abstract::Moloney murine leukemia virus (M-MuLV) is capable of inducing promonocytic leukemia in 50% of adult BALB/c mice that have received peritoneal injections of pristane, but Friend MuLV strain 57 (F-MuLV) is nonleukemogenic under similar conditions. It was shown earlier that these differences could not be mapped to the U3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5100-5107.1994
更新日期:1994-08-01 00:00:00
abstract::Understanding the role of host factors during lethal influenza virus infection is critical to deciphering the events that determine the fate of the host. One such factor is encoded by the Mx1 gene, which confers resistance to influenza virus infection. Here, we compared pathology and global gene expression profiles in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06156-11
更新日期:2012-03-01 00:00:00
abstract::Molluscum contagiosum virus (MCV), a member of the human poxvirus family, encodes the MC159 protein that inhibits Fas-, tumor necrosis factor (TNF)-, and TNF-related apoptosis-inducing ligant (TRAIL)-induced apoptosis. We used site-directed mutagenesis to change charged or hydrophobic amino acid residues to alanines t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.2.697-706.2002
更新日期:2002-01-01 00:00:00
abstract::We have devised an enrichment scheme for the isolation of export-competent derivatives of pseudorabies virus glycoprotein gIII signal peptide mutants. Enrichment is based upon a growth advantage imparted upon gIII-containing virions compared with virions lacking the glycoprotein. Each of identified derivatives suppres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.2787-2794.1994
更新日期:1994-05-01 00:00:00
abstract::Viral particles infecting some stocks of the protozoan parasite Leishmania braziliensis subsp. guyanensis contain a double-stranded RNA genome of ca. 5 kbp and are associated with an RNA-dependent RNA polymerase which synthesizes in vitro double-stranded and single-stranded, genome-length transcripts. The majority of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4211-4215.1991
更新日期:1991-08-01 00:00:00
abstract::A region in addition to and outside the long terminal repeats (LTRs) in the gag gene of the Prague A strain of Rous sarcoma virus was found to be essential in cis for efficient cell transformation by cloned viral DNA. Transformation in chicken embryo fibroblasts, which requires infectious virus production and reinfect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.11.3401-3409.1987
更新日期:1987-11-01 00:00:00
abstract::Adenovirus class B (Ad3 and Ad7) and class C (Ad1, Ad5, and Ad6) late r-strand mRNA's were found to have segmented 5' leaders. These leaders were very similar among serotype within a class but differed in sequence from the leaders on late mRNA's of a different class. However, the leader components of class B viruses m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.3.899-912.1979
更新日期:1979-06-01 00:00:00
abstract::Influenza virus hemagglutinin consists of a highly variable and immunodominant head domain and a more conserved but immunosubdominant stalk domain. We introduced seven N-linked glycosylation sites in the hemagglutinin head domain to shield the immunodominant antigenic sites. The hyperglycosylated hemagglutinin enhance...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02608-13
更新日期:2014-01-01 00:00:00
abstract:UNLABELLED:Susceptibility to alphavirus infection is age dependent, and host maturation is associated with decreased virus replication and less severe encephalitis. To identify factors associated with maturation-dependent restriction of virus replication, we studied AP-7 rat olfactory bulb neuronal cells, which can dif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02394-14
更新日期:2015-01-01 00:00:00
abstract::Nonstructural protein 5A (NS5A) is a membrane-associated essential component of the hepatitis C virus (HCV) replication complex. An N-terminal amphipathic alpha helix mediates in-plane membrane association of HCV NS5A and at the same time is likely involved in specific protein-protein interactions required for the ass...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01279-06
更新日期:2007-03-01 00:00:00