Abstract:
:The tegument of herpesviruses is a highly complex structural layer between the nucleocapsid and the envelope of virions. Tegument proteins play both structural and regulatory functions during replication and spread, but the interactions and functions of many of these proteins are poorly understood. Here we focus on two tegument proteins from herpes simplex virus 1 (HSV-1), pUL7 and pUL51, which have homologues in all other herpesviruses. We have now identified that HSV-1 pUL7 and pUL51 form a stable and direct protein-protein interaction, their expression levels rely on the presence of each other, and they function as a complex in infected cells. We demonstrate that expression of the pUL7-pUL51 complex is important for efficient HSV-1 assembly and plaque formation. Furthermore, we also discovered that the pUL7-pUL51 complex localizes to focal adhesions at the plasma membrane in both infected cells and in the absence of other viral proteins. The expression of pUL7-pUL51 is important to stabilize focal adhesions and maintain cell morphology in infected cells and cells infected with viruses lacking pUL7 and/or pUL51 round up more rapidly than cells infected with wild-type HSV-1. Our data suggest that, in addition to the previously reported functions in virus assembly and spread for pUL51, the pUL7-pUL51 complex is important for maintaining the attachment of infected cells to their surroundings through modulating the activity of focal adhesion complexes. IMPORTANCE:Herpesviridae is a large family of highly successful human and animal pathogens. Virions of these viruses are composed of many different proteins, most of which are contained within the tegument, a complex structural layer between the nucleocapsid and the envelope within virus particles. Tegument proteins have important roles in assembling virus particles as well as modifying host cells to promote virus replication and spread. However, little is known about the function of many tegument proteins during virus replication. Our study focuses on two tegument proteins from herpes simplex virus 1 that are conserved in all herpesviruses: pUL7 and pUL51. We demonstrate that these proteins directly interact and form a functional complex that is important for both virus assembly and modulation of host cell morphology. Further, we identify for the first time that these conserved herpesvirus tegument proteins localize to focal adhesions in addition to cytoplasmic juxtanuclear membranes within infected cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Albecka A,Owen DJ,Ivanova L,Brun J,Liman R,Davies L,Ahmed MF,Colaco S,Hollinshead M,Graham SC,Crump CMdoi
10.1128/JVI.02196-16subject
Has Abstractpub_date
2017-01-03 00:00:00issue
2eissn
0022-538Xissn
1098-5514pii
JVI.02196-16journal_volume
91pub_type
杂志文章abstract::Infectious bursal disease virus (IBDV) is a double-stranded RNA (dsRNA) virus of the Birnaviridae family. Its two genome segments are encapsidated together with multiple copies of the viral RNA-dependent RNA polymerase, VP1, in a single-shell capsid that is composed of VP2 and VP3. In this study we identified the doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11301-11311.2002
更新日期:2002-11-01 00:00:00
abstract::We molecularly cloned unintegrated viral DNA of the BALB/c endogenous N-tropic and B-tropic murine leukemia retroviruses and in vitro passaged N-tropic Gross (passage A) murine leukemia retroviruses. Recombinant genomes were constructed in vitro by exchanging homologous restriction enzyme fragments from N- or B-tropic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.110-119.1983
更新日期:1983-10-01 00:00:00
abstract::Moloney murine leukemia virus (M-MuLV) is capable of inducing promonocytic leukemia in 50% of adult BALB/c mice that have received peritoneal injections of pristane, but Friend MuLV strain 57 (F-MuLV) is nonleukemogenic under similar conditions. It was shown earlier that these differences could not be mapped to the U3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5100-5107.1994
更新日期:1994-08-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) establishes a persistent infection in the host despite a vigorous virus-specific immune response. Here we demonstrate that an HTLV-1-encoded protein, p12(I), resides in the endoplasmic reticulum (ER) and Golgi and physically binds to the free human major histocompatibility c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.6086-6094.2001
更新日期:2001-07-01 00:00:00
abstract::We have developed a high-throughput sequencing (HTS) workflow for investigating paramyxovirus transcription and replication. We show that sequencing of oligo(dT)-selected polyadenylated mRNAs, without considering the orientation of the RNAs from which they had been generated, cannot accurately be used to analyze the a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00571-19
更新日期:2019-08-13 00:00:00
abstract::We isolated a simian virus 40 mutant, dl2194, which carried deletions in both early genes. One deletion removed 234 base pairs in the 54/59 region within the small-t-antigen coding sequence and the large-T-antigen gene intron. The second deletion removed 57 base pairs at the C terminus of the large-T-antigen coding se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.625-634.1981
更新日期:1981-12-01 00:00:00
abstract::We investigated the use of infectious cDNA for the production of poliovirus type 1-type 3 recombinants. One such recombinant virus was produced, but a second construct involving the transfer of part of the capsid protein region was not infectious. Our results suggest that the approach may prove valuable but that not a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.1187-1190.1986
更新日期:1986-03-01 00:00:00
abstract::A putative transcription factor, C/EBP, isolated from rat liver nuclei, has been shown to bind to at least two different sequence motifs: the CCAAT promoter domain and a core sequence [GTGG(T/A)(T/A)(T/A)G] common to many viral enhancers, including simian virus 40 and human hepatitis B virus. It has been proposed that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.4.1517-1522.1990
更新日期:1990-04-01 00:00:00
abstract::The regulatory proteins Nef, Rev, and Tat of human immunodeficiency virus type 1 (HIV-1) are attractive targets for vaccine development, since induction of effective immune responses targeting these early proteins may best control virus replication. Here we investigated whether vaccination with biologically active Tat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3800-3809.2002
更新日期:2002-04-01 00:00:00
abstract::For efficient and versatile use of adenovirus (Ad) as an in vivo gene therapy vector, modulation of the viral tropism is highly desirable. In this study, a novel method to genetically alter the Ad fiber tropism is described. The knob and the last 15 shaft repeats of the fiber gene were deleted and replaced with an ext...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7280-7289.2001
更新日期:2001-08-01 00:00:00
abstract::Antisera were prepared against the amino acid sequences encoded within the N-terminal half of the adenovirus 12 (Ad12) early region 1A (E1A) gene. This was accomplished by construction of a plasmid vector which encoded the N-terminal 131 amino acids of Ad12 E1A joined in frame to the coding sequence of beta-galactosid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.895-903.1984
更新日期:1984-06-01 00:00:00
abstract::An effective vaccine for AIDS may require development of novel vectors capable of eliciting long-lasting immune responses. Here we report the development and use of replication-competent and replication-defective strains of recombinant herpes simplex virus (HSV) that express envelope and Nef antigens of simian immunod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7745-7754.2000
更新日期:2000-09-01 00:00:00
abstract::A human endogenous retrovirus-like element (HERV), flanked by long terminal repeats of 502 and 495 nucleotides is inserted into the human pleiotrophin (PTN) gene upstream of the open reading frame. Based on its Glu-tRNA primer binding site specificity and the location within the PTN gene, we named this element HERV-E....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.6065-6072.1998
更新日期:1998-07-01 00:00:00
abstract::Nine virulent bacteriophages of the anaerobe Corynebacterium (Propionibacterium) acnes, the P-a series, are DNA phages, with long, curved nonretractile tails (130 nm) without tail plates or fibers. They have isometric heads (420 by 460 nm), and are placed in Bradley's group B-1. There is permanent plaque suppression a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1268-1273.1974
更新日期:1974-11-01 00:00:00
abstract::BALB/c mice treated with pristane and Abelson virus have been used as an animal model system for the rapid induction of plasmacytomas. Myelomonocytic tumors with helper Moloney murine leukemia virus clonally inserted into the c-myb locus were observed in about 10% of pristane-primed BALB/c mice infected with Abelson v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3721-3725.1987
更新日期:1987-12-01 00:00:00
abstract::Recombinant viruses possessing reporter proteins have been generated for virus research. In the case of the family Flaviviridae, we recently generated recombinant viruses, including the hepatitis C virus of the genus Hepacivirus, Japanese encephalitis virus (JEV) of the genus Flavivirus, and bovine viral diarrhea viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01191-19
更新日期:2019-10-29 00:00:00
abstract::Resistance to ectromelia (mousepox) virus-induced mortality was examined in crosses between susceptible DBA/2J, A/J, and BALB/cByJ mice and resistant C57BL/6J and AKR/J mice. Depending on the cross, resistance to mousepox virus was shown to be determined by one or more independently assorting autosomal loci with domin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.890-891.1985
更新日期:1985-09-01 00:00:00
abstract::Pancreatic islets from DBA/2 mice infected with the D variant of encephalomyocarditis (EMC-D) virus revealed lymphocytic infiltration with moderate to severe destruction of pancreatic beta cells. Our previous studies showed that the major population of infiltrating cells at the early stages of infection is macrophages...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.4024-4031.1997
更新日期:1997-05-01 00:00:00
abstract::Myeloblastosis-associated virus (MAV)-2(0), a virus which was derived from avian myeloblastosis virus and induced a high incidence of osteopetrosis, was compared with avian lymphomatosis virus 5938, a recent field isolate which induced a high incidence of lymphomatosis. The following information was obtained. (i) MAV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.160-167.1976
更新日期:1975-01-01 00:00:00
abstract::RNA-dependent protein kinase PKR is an important regulator of gene expression in interferon (IFN)-treated and virus-infected cells. The 50-kb gene encoding human PKR kinase (pkr) is inducible by IFN. Transfection analyses, using chloramphenicol acetyltransferase (CAT) as the reporter in constructs possessing various 5...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9934-9939.1998
更新日期:1998-12-01 00:00:00
abstract::Repeated passages of vaccinia virus in increasing concentrations of hydroxyurea followed by plaque purification resulted in the isolation of variants capable of growth in 5 mM hydroxyurea, a drug concentration which inhibited the reproduction of wild-type vaccinia virus 1,000-fold. Analyses of viral protein synthesis ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.506-514.1986
更新日期:1986-11-01 00:00:00
abstract::Nucleotide substitution mutagenesis was conducted to investigate the importance of the inverted repeats (palindrome) at the origin of DNA replication (Ori) of porcine circovirus type 1 (PCV1). Viral genomes with engineered mutations on either arm or both arms of the palindrome were not impaired in protein synthesis an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.8.4268-4277.2004
更新日期:2004-04-01 00:00:00
abstract::Recently, we showed that the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) VP80 protein is essential for the formation of both virion types, budded virus (BV) and occlusion-derived virus (ODV). Deletion of the vp80 gene did not affect assembly of nucleocapsids. However, these nucleocapsids were not ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00035-11
更新日期:2011-06-01 00:00:00
abstract:UNLABELLED:Since the 1960s, simian hemorrhagic fever virus (SHFV; Nidovirales, Arteriviridae) has caused highly fatal outbreaks of viral hemorrhagic fever in captive Asian macaque colonies. However, the source(s) of these outbreaks and the natural reservoir(s) of this virus remain obscure. Here we report the identifica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02203-14
更新日期:2014-11-01 00:00:00
abstract::Hamster cells are completely nonpermissive for the replication of human adenovirus type 12 (Ad12), whereas types 2 and 5 can replicate in hamster cells. The Ad5-transformed hamster cell line BHK297-C131, which carries the left terminal 18.7% of the Ad5 genome and expresses at least the viral E1A region, can somehow co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5476-5482.1994
更新日期:1994-09-01 00:00:00
abstract::The activity and epithelial tropism of the human papillomavirus type 18 P105 early promoter, which directs the synthesis of the E6 and E7 transforming genes, are controlled by cis elements included in the viral long control region. To identify potential cellular regulators of this promoter, we mutagenized one or both ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3740-3748.1992
更新日期:1992-06-01 00:00:00
abstract::GP64, the major envelope glycoprotein of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) budded virion, is important for host cell receptor binding and mediates low-pH-triggered membrane fusion during entry by endocytosis. In the current study, we examined the functional role of the AcMNPV GP64 tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02104-07
更新日期:2008-04-01 00:00:00
abstract::Adeno-associated virus (AAV) integrates very efficiently into a specific site (AAVS1) of human chromosome 19. Two elements of the AAV genome are sufficient: the inverted terminal repeats (ITRs) and the Rep78 or Rep68 protein. The incorporation of the AAV integration machinery in nonviral delivery systems is of great i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7653-7658.1998
更新日期:1998-09-01 00:00:00
abstract:UNLABELLED:We searched The Cancer Genome Atlas (TCGA) database for viruses by comparing non-human reads present in transcriptome sequencing (RNA-Seq) and whole-exome sequencing (WXS) data to viral sequence databases. Human papillomavirus 18 (HPV18) is an etiologic agent of cervical cancer, and as expected, we found rob...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03365-14
更新日期:2015-04-01 00:00:00
abstract::The fundamental question of whether a primed immune system is capable of preventing latent gammaherpesvirus infection remains unanswered. Recent studies showing that vaccination can reduce acute replication and short-term latency but cannot alter long-term latency further call into question the possibility of achievin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2522-2529.2003
更新日期:2003-02-01 00:00:00